(Circular) (Six-base) MAPSORT of: pFN21ASDA0759.seq Check: 9684 from: 1 to: 6739 pFN21ASDA0759 6739 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3749 4549 278 Size: 53 83 186 936 2213 800 2468 Fragments arranged by size: 2468 2213 936 800 186 83 53 AccI GT'mk_AC Cuts at: 1937 4082 1937 Size: 2145 4594 Acc65I G'GTAC_C Cuts at: 3719 4060 3719 Size: 341 6398 AclI AA'CG_TT Cuts at: 1563 4866 5239 1563 Size: 3303 373 3063 Fragments arranged by size: 3303 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2319 2747 3954 3966 4802 6186 6454 1632 Size: 687 428 1207 12 836 1384 268 1917 Fragments arranged by size: 1917 1384 1207 836 687 428 268 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6542 AflIII A'CryG_T Cuts at: 1203 2099 5638 6615 1203 Size: 896 3539 977 1327 Fragments arranged by size: 3539 1327 977 896 AgeI A'CCGG_T Cuts at: 5570 5570 Size: 6739 AhdI GACnn_n'nnGTC Cuts at: 2347 5468 2347 Size: 3121 3618 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6739 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6739 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2117 2149 2117 Size: 32 6707 AlwNI CAG_nnn'CTG Cuts at: 2725 2791 3297 4482 6054 2725 Size: 66 506 1185 1572 3410 Fragments arranged by size: 3410 1572 1185 506 66 ApaI G_GGCC'C Cuts at: 2177 2262 2177 Size: 85 6654 ApaLI G'TGCA_C Cuts at: 4796 5952 4796 Size: 1156 5583 ApoI r'AATT_y Cuts at: 1493 2673 2876 3325 4048 4274 4426 6535 1493 Size: 1180 203 449 723 226 152 2109 1697 Fragments arranged by size: 2109 1697 1180 723 449 226 203 152 AseI AT'TA_AT Cuts at: 160 1032 5293 160 Size: 872 4261 1606 Fragments arranged by size: 4261 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 AvaI C'yCGr_G Cuts at: 1870 1943 2169 4064 1870 Size: 73 226 1895 4545 Fragments arranged by size: 4545 1895 226 73 BamHI G'GATC_C Cuts at: 4069 4436 4069 Size: 367 6372 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2522 3719 4060 5515 618 Size: 325 217 627 735 1197 341 1455 1842 Fragments arranged by size: 1842 1455 1197 735 627 341 325 217 BanII G_rGCy'C Cuts at: 1391 2177 2262 2456 2490 2807 2814 3375 4058 Size: 786 85 194 34 317 7 561 683 Cuts at: 4058 1391 Size: 4072 Fragments arranged by size: 4072 786 683 561 317 194 85 34 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3423 928 Size: 971 1524 4244 Fragments arranged by size: 4244 1524 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2999 3033 4930 4964 1903 Size: 34 1062 34 1897 34 3678 Fragments arranged by size: 3678 1897 1062 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4631 5841 4631 Size: 1210 5529 BclI T'GATC_A Cuts at: 1548 3874 1548 Size: 2326 4413 BfrBI ATG'CAT Cuts at: 3683 3683 Size: 6739 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3242 5350 136 Size: 107 122 71 631 605 1570 2108 1525 Fragments arranged by size: 2108 1570 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6734 6734 Size: 6739 BlpI GC'TnA_GC Cuts at: 4156 4156 Size: 6739 Bme1580I G_kGCm'C Cuts at: 1790 2177 2262 2527 2595 4800 5956 1790 Size: 387 85 265 68 2205 1156 2573 Fragments arranged by size: 2573 2205 1156 387 265 85 68 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6739 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2485 2510 2896 Size: 416 53 83 399 24 1068 25 386 Cuts at: 2896 2944 3006 4470 5428 442 Size: 48 62 1464 958 1753 Fragments arranged by size: 1753 1464 1068 958 416 399 386 83 62 53 48 25 24 BmtI G_CTAG'C Cuts at: 1056 3063 4004 1056 Size: 2007 941 3791 Fragments arranged by size: 3791 2007 941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2716 2716 Size: 6739 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 2453 2590 2894 3359 3691 5399 1239 Size: 1098 116 137 304 465 332 1708 2579 Fragments arranged by size: 2579 1708 1098 465 332 304 137 116 Bpu10I CC'TnA_GC Cuts at: 2178 2583 2718 2784 2178 Size: 405 135 66 6133 Fragments arranged by size: 6133 405 135 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3692 3713 3887 4864 5729 6027 3692 Size: 21 174 977 865 298 4404 Fragments arranged by size: 4404 977 865 298 174 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5402 882 Size: 617 3903 2219 Fragments arranged by size: 3903 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4036 4530 493 Size: 711 2832 494 2702 Fragments arranged by size: 2832 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6733 6733 Size: 6739 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3746 4546 4928 5563 Size: 53 83 186 936 2213 800 382 635 Cuts at: 5563 275 Size: 1451 Fragments arranged by size: 2213 1451 936 800 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5172 5570 5844 5991 6402 5172 Size: 398 274 147 411 5509 Fragments arranged by size: 5509 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 1178 Size: 30 165 30 1074 30 5410 Fragments arranged by size: 5410 1074 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 3004 1186 Size: 1249 569 4921 Fragments arranged by size: 4921 1249 569 BseYI C'CCAG_C Cuts at: 2554 2706 3467 3771 5942 6438 2554 Size: 152 761 304 2171 496 2855 Fragments arranged by size: 2855 2171 761 496 304 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6166 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4189 4950 5978 1540 Size: 275 186 2188 761 1028 2301 Fragments arranged by size: 2301 2188 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2241 2807 2814 3375 4800 4885 5956 2241 Size: 566 7 561 1425 85 1071 3024 Fragments arranged by size: 3024 1425 1071 566 561 85 7 BsmI GAATG_Cn' Cuts at: 2332 4250 4343 2332 Size: 1918 93 4728 Fragments arranged by size: 4728 1918 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3526 3923 3526 Size: 397 6342 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2241 2262 2456 2490 2527 2595 Size: 399 387 64 21 194 34 37 68 Cuts at: 2595 2807 2814 3375 4058 4800 4885 5956 1391 Size: 212 7 561 683 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 561 399 387 212 194 85 68 64 37 34 21 7 BspEI T'CCGG_A Cuts at: 6402 6402 Size: 6739 BspHI T'CATG_A Cuts at: 2668 4628 6358 2668 Size: 1960 1730 3049 Fragments arranged by size: 3049 1960 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2055 3655 4094 844 Size: 1211 1600 439 3489 Fragments arranged by size: 3489 1600 1211 439 BsrBI CCG'CTC Cuts at: 1394 1823 4626 1394 Size: 429 2803 3507 Fragments arranged by size: 3507 2803 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2821 5234 5408 65 Size: 1183 1573 2413 174 1396 Fragments arranged by size: 2413 1573 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2056 5383 5570 1700 Size: 110 246 3327 187 2869 Fragments arranged by size: 3327 2869 246 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6739 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6739 BssSI C'ACGA_G Cuts at: 4799 5811 4799 Size: 1012 5727 BstAPI GCAn_nnn'nTGC Cuts at: 794 2504 3297 794 Size: 1710 793 4236 Fragments arranged by size: 4236 1710 793 BstBI TT'CG_AA Cuts at: 2007 5543 2007 Size: 3536 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6739 BstXI CCAn_nnnn'nTGG Cuts at: 3253 3383 3475 3253 Size: 130 92 6517 Fragments arranged by size: 6517 130 92 BstYI r'GATC_y Cuts at: 1972 4069 4436 4821 4838 6290 6376 6388 6734 Size: 2097 367 385 17 1452 86 12 346 Cuts at: 6734 1972 Size: 1977 Fragments arranged by size: 2097 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2160 3382 513 Size: 552 1095 1222 3870 Fragments arranged by size: 3870 1222 1095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3237 3565 487 Size: 683 56 2011 328 3661 Fragments arranged by size: 3661 2011 683 328 56 BtsI GCAGTG_nn' Cuts at: 2216 2426 2546 2658 3295 3730 4255 5049 5069 Size: 210 120 112 637 435 525 794 20 Cuts at: 5069 2216 Size: 3886 Fragments arranged by size: 3886 794 637 525 435 210 120 112 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6739 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6704 DraI TTT'AAA Cuts at: 4043 4043 Size: 6739 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5746 809 Size: 507 4430 1802 Fragments arranged by size: 4430 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2529 3243 Size: 54 1425 50 275 18 228 471 714 Cuts at: 3243 3273 3544 3778 4186 5075 6704 8 Size: 30 271 234 408 889 1629 43 Fragments arranged by size: 1629 1425 889 714 471 408 275 271 234 228 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 4186 1537 Size: 275 2374 4090 Fragments arranged by size: 4090 2374 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2316 2541 2595 3930 3985 4669 1353 Size: 722 241 225 54 1335 55 684 3423 Fragments arranged by size: 3423 1335 722 684 241 225 55 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3035 3272 5326 5700 5846 387 Size: 1064 138 1446 237 2054 374 146 1280 Fragments arranged by size: 2054 1446 1280 1064 374 237 146 138 EcoICRI GAG'CTC Cuts at: 2805 2812 3373 2805 Size: 7 561 6171 Fragments arranged by size: 6171 561 7 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 2337 2453 2590 2747 2894 3359 Size: 393 687 18 116 137 157 147 465 Cuts at: 3359 3691 3954 3966 4802 5399 6186 6454 1239 Size: 332 263 12 836 597 787 268 1524 Fragments arranged by size: 1524 836 787 687 597 465 393 332 268 263 157 147 137 116 18 12 EcoNI CCTnn'n_nnAGG Cuts at: 2783 2870 3706 2783 Size: 87 836 5816 Fragments arranged by size: 5816 836 87 EcoO109I rG'GnC_Cy Cuts at: 2173 2174 2197 2258 2259 2551 2626 2722 2788 Size: 1 23 61 1 292 75 96 66 Cuts at: 2788 2890 3319 3454 2173 Size: 102 429 135 5458 Fragments arranged by size: 5458 429 292 135 102 96 75 66 61 23 1 1 EcoRI G'AATT_C Cuts at: 2876 4048 4426 2876 Size: 1172 378 5189 Fragments arranged by size: 5189 1172 378 EcoRV GAT'ATC Cuts at: 2899 2899 Size: 6739 FspI TGC'GCA Cuts at: 5245 5245 Size: 6739 HaeII r_GCGC'y Cuts at: 1678 5886 1678 Size: 4208 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2296 2328 2333 2365 5380 Size: 32 135 32 724 32 5 32 3015 Cuts at: 5380 5412 5454 5486 1373 Size: 32 42 32 2626 Fragments arranged by size: 3015 2626 724 135 42 32 32 32 32 32 32 5 HincII GTy'rAC Cuts at: 669 1657 1938 4083 4329 669 Size: 988 281 2145 246 3079 Fragments arranged by size: 3079 2145 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6739 HpaI GTT'AAC Cuts at: 4329 4329 Size: 6739 KpnI G_GTAC'C Cuts at: 3723 4064 3723 Size: 341 6398 MfeI C'AATT_G Cuts at: 2842 4338 2842 Size: 1496 5243 MluI A'CGCG_T Cuts at: 6615 6615 Size: 6739 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2463 5853 6037 1305 Size: 1158 3390 184 2007 Fragments arranged by size: 3390 2007 1158 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2531 3245 3275 10 Size: 54 1425 343 699 714 30 3474 Fragments arranged by size: 3474 1425 714 699 343 54 30 MslI CAynn'nnrTG Cuts at: 518 1166 2762 3797 4697 5056 5215 518 Size: 648 1596 1035 900 359 159 2042 Fragments arranged by size: 2042 1596 1035 900 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 2558 2944 3178 3845 4155 4832 5980 Size: 756 196 386 234 667 310 677 1148 Cuts at: 5980 6225 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 756 677 667 386 310 245 234 196 NaeI GCC'GGC Cuts at: 2058 2058 Size: 6739 NcoI C'CATG_G Cuts at: 513 1065 2160 3382 513 Size: 552 1095 1222 3870 Fragments arranged by size: 3870 1222 1095 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6739 NgoMIV G'CCGG_C Cuts at: 2056 2056 Size: 6739 NheI G'CTAG_C Cuts at: 1052 3059 4000 1052 Size: 2007 941 3791 Fragments arranged by size: 3791 2007 941 NotI GC'GGCC_GC Cuts at: 4186 4186 Size: 6739 NsiI A_TGCA'T Cuts at: 3685 3685 Size: 6739 NspI r_CATG'y Cuts at: 1132 1229 2103 4097 5642 1132 Size: 97 874 1994 1545 2229 Fragments arranged by size: 2229 1994 1545 874 97 PasI CC'CwG_GG Cuts at: 1348 3475 1348 Size: 2127 4612 PciI A'CATG_T Cuts at: 2099 5638 2099 Size: 3539 3200 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6739 PfoI T'CCnGG_A Cuts at: 2314 2910 2919 3707 2314 Size: 596 9 788 5346 Fragments arranged by size: 5346 788 596 9 PmeI GTTT'AAAC Cuts at: 4043 4043 Size: 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4808 4840 6321 6353 4808 Size: 32 1481 32 5194 Fragments arranged by size: 5194 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2551 2788 2890 3319 2551 Size: 237 102 429 5971 Fragments arranged by size: 5971 429 237 102 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6739 PsiI TTA'TAA Cuts at: 2772 4309 2772 Size: 1537 5202 PspOMI G'GGCC_C Cuts at: 2173 2258 2173 Size: 85 6654 PstI C_TGCA'G Cuts at: 830 2208 2383 3453 3578 4091 830 Size: 1378 175 1070 125 513 3478 Fragments arranged by size: 3478 1378 1070 513 175 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6739 PvuII CAG'CTG Cuts at: 2362 2944 3845 2362 Size: 582 901 5256 Fragments arranged by size: 5256 901 582 SacI G_AGCT'C Cuts at: 2807 2814 3375 2807 Size: 7 561 6171 Fragments arranged by size: 6171 561 7 SalI G'TCGA_C Cuts at: 1936 4081 1936 Size: 2145 4594 SanDI GG'GwC_CC Cuts at: 2890 2890 Size: 6739 SapI GCTCTTCn'nnn_ Cuts at: 3985 3985 Size: 6739 SbfI CC_TGCA'GG Cuts at: 4091 4091 Size: 6739 ScaI AGT'ACT Cuts at: 3074 4987 6699 3074 Size: 1913 1712 3114 Fragments arranged by size: 3114 1913 1712 SfcI C'TryA_G Cuts at: 826 1045 2204 2379 3449 3574 3714 4087 5222 Size: 219 1159 175 1070 125 140 373 1135 Cuts at: 5222 5903 6094 826 Size: 681 191 1471 Fragments arranged by size: 1471 1159 1135 1070 681 373 219 191 175 140 125 SfiI GGCCn_nnn'nGGCC Cuts at: 3242 3242 Size: 6739 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6739 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6739 SmaI CCC'GGG Cuts at: 1872 4066 1872 Size: 2194 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3671 3692 3902 4843 5744 6006 Size: 197 926 1728 21 210 941 901 262 Cuts at: 6006 820 Size: 1553 Fragments arranged by size: 1728 1553 941 926 901 262 210 197 21 SnaBI TAC'GTA Cuts at: 493 4036 4530 493 Size: 3543 494 2702 Fragments arranged by size: 3543 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6739 SphI G_CATG'C Cuts at: 1132 4097 1132 Size: 2965 3774 SspI AAT'ATT Cuts at: 5 52 4663 5 Size: 47 4611 2081 Fragments arranged by size: 4611 2081 47 StuI AGG'CCT Cuts at: 1517 3153 1517 Size: 1636 5103 StyI C'CwwG_G Cuts at: 513 1065 2160 3382 4178 513 Size: 552 1095 1222 796 3074 Fragments arranged by size: 3074 1222 1095 796 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2510 2720 2988 4766 4783 4936 5121 Size: 1255 930 210 268 1778 17 153 185 Cuts at: 5121 5566 325 Size: 445 1498 Fragments arranged by size: 1778 1498 1255 930 445 268 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3072 4985 6697 Size: 274 80 33 51 1443 1095 1913 1712 Cuts at: 6697 96 Size: 138 Fragments arranged by size: 1913 1712 1443 1095 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6699 6731 6699 Size: 32 6707 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6739 XbaI T'CTAG_A Cuts at: 4075 4075 Size: 6739 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4012 4012 Size: 6739 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6739 XmaI C'CCGG_G Cuts at: 1870 4064 1870 Size: 2194 4545 XmnI GAAnn'nnTTC Cuts at: 4868 4868 Size: 6739 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3747 4547 276 Size: 53 83 186 936 2213 800 2468 Fragments arranged by size: 2468 2213 936 800 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SacII SexAI SfoI SrfI SwaI