(Circular) (Six-base) MAPSORT of: pFN21ASDA0758.seq Check: 9935 from: 1 to: 8767 pFN21ASDA0758 8767 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3047 5186 3047 Size: 2139 6628 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2454 3702 6577 278 Size: 53 83 186 936 918 1248 2875 2468 Fragments arranged by size: 2875 2468 1248 936 918 186 83 53 AccI GT'mk_AC Cuts at: 1937 2108 6110 1937 Size: 171 4002 4594 Fragments arranged by size: 4594 4002 171 Acc65I G'GTAC_C Cuts at: 6088 6088 Size: 8767 AclI AA'CG_TT Cuts at: 1563 4095 6894 7267 1563 Size: 2532 2799 373 3063 Fragments arranged by size: 3063 2799 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2504 4289 4848 4882 5855 6830 8214 Size: 591 281 1785 559 34 973 975 1384 Cuts at: 8214 8482 1632 Size: 268 1917 Fragments arranged by size: 1917 1785 1384 975 973 591 559 281 268 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8570 AflIII A'CryG_T Cuts at: 1203 7666 8643 1203 Size: 6463 977 1327 Fragments arranged by size: 6463 1327 977 AgeI A'CCGG_T Cuts at: 7598 7598 Size: 8767 AhdI GACnn_n'nnGTC Cuts at: 7496 7496 Size: 8767 AleI CACnn'nnGTG Cuts at: 1166 5594 5735 5924 1166 Size: 4428 141 189 4009 Fragments arranged by size: 4428 4009 189 141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8767 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2576 2608 2618 2650 2576 Size: 32 10 32 8693 Fragments arranged by size: 8693 32 32 10 AlwNI CAG_nnn'CTG Cuts at: 6510 8082 6510 Size: 1572 7195 ApaLI G'TGCA_C Cuts at: 6824 7980 6824 Size: 1156 7611 ApoI r'AATT_y Cuts at: 1493 2652 3135 3673 4384 6076 6302 6454 8563 Size: 1159 483 538 711 1692 226 152 2109 Cuts at: 8563 1493 Size: 1697 Fragments arranged by size: 2109 1697 1692 1159 711 538 483 226 152 AseI AT'TA_AT Cuts at: 160 1032 7321 160 Size: 872 6289 1606 Fragments arranged by size: 6289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8767 AvaI C'yCGr_G Cuts at: 1870 1943 2409 5478 6092 1870 Size: 73 466 3069 614 4545 Fragments arranged by size: 4545 3069 614 466 73 BamHI G'GATC_C Cuts at: 2254 4033 6097 6464 2254 Size: 1779 2064 367 4557 Fragments arranged by size: 4557 2064 1779 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2466 6088 7543 618 Size: 325 217 627 679 3622 1455 1842 Fragments arranged by size: 3622 1842 1455 679 627 325 217 BanII G_rGCy'C Cuts at: 1391 3986 4238 6086 1391 Size: 2595 252 1848 4072 Fragments arranged by size: 4072 2595 1848 252 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 2414 2446 2580 4879 5314 5782 Size: 971 221 294 32 134 2299 435 468 Cuts at: 5782 5874 928 Size: 92 3821 Fragments arranged by size: 3821 2299 971 468 435 294 221 134 92 32 BbvCI CC'TCA_GC Cuts at: 5315 5315 Size: 8767 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6958 6992 1903 Size: 34 5021 34 3678 Fragments arranged by size: 5021 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6659 7869 6659 Size: 1210 7557 BclI T'GATC_A Cuts at: 1548 4227 5562 1548 Size: 2679 1335 4753 Fragments arranged by size: 4753 2679 1335 BfrBI ATG'CAT Cuts at: 2243 3498 2243 Size: 1255 7512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4866 5282 5540 Size: 107 122 71 631 605 3194 416 258 Cuts at: 5540 7378 136 Size: 1838 1525 Fragments arranged by size: 3194 1838 1525 631 605 416 258 122 107 71 BglII A'GATC_T Cuts at: 8762 8762 Size: 8767 BlpI GC'TnA_GC Cuts at: 6184 6184 Size: 8767 Bme1580I G_kGCm'C Cuts at: 1790 2469 3332 6828 7984 1790 Size: 679 863 3496 1156 2573 Fragments arranged by size: 3496 2573 1156 863 679 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3319 4150 5351 Size: 416 53 83 399 24 1902 831 1201 Cuts at: 5351 5527 6498 7456 442 Size: 176 971 958 1753 Fragments arranged by size: 1902 1753 1201 971 958 831 416 399 176 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4257 4257 Size: 8767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3030 4193 7427 1239 Size: 1791 1163 3234 2579 Fragments arranged by size: 3234 2579 1791 1163 Bpu10I CC'TnA_GC Cuts at: 4218 4752 5315 4218 Size: 534 563 7670 Fragments arranged by size: 7670 563 534 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2765 3293 5116 6892 7757 8055 2765 Size: 528 1823 1776 865 298 3477 Fragments arranged by size: 3477 1823 1776 865 528 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5732 7430 882 Size: 617 4233 1698 2219 Fragments arranged by size: 4233 2219 1698 617 BsaAI yAC'GTr Cuts at: 493 1204 4405 6064 6558 493 Size: 711 3201 1659 494 2702 Fragments arranged by size: 3201 2702 1659 711 494 BsaBI GATnn'nnATC Cuts at: 8761 8761 Size: 8767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2451 3699 6574 6956 Size: 53 83 186 936 918 1248 2875 382 Cuts at: 6956 7591 275 Size: 635 1451 Fragments arranged by size: 2875 1451 1248 936 918 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2637 7200 7598 7872 8019 8430 2637 Size: 4563 398 274 147 411 2974 Fragments arranged by size: 4563 2974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2576 2606 2798 2828 1178 Size: 30 165 30 1173 30 192 30 7117 Fragments arranged by size: 7117 1173 192 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3595 3815 4178 1186 Size: 2409 220 363 5775 Fragments arranged by size: 5775 2409 363 220 BseYI C'CCAG_C Cuts at: 4100 5461 5472 5554 7970 8466 4100 Size: 1361 11 82 2416 496 4401 Fragments arranged by size: 4401 2416 1361 496 82 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2070 4595 1163 Size: 573 334 2525 5335 Fragments arranged by size: 5335 2525 573 334 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6217 6978 8006 1540 Size: 275 186 4216 761 1028 2301 Fragments arranged by size: 4216 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2384 4410 6828 6913 7984 2384 Size: 2026 2418 85 1071 3167 Fragments arranged by size: 3167 2418 2026 1071 85 BsmI GAATG_Cn' Cuts at: 6278 6371 6278 Size: 93 8674 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2384 2469 3332 3986 4238 4410 6086 Size: 399 594 85 863 654 252 172 1676 Cuts at: 6086 6828 6913 7984 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1676 1071 863 742 654 594 399 252 172 85 85 BspEI T'CCGG_A Cuts at: 2637 8430 2637 Size: 5793 2974 BspHI T'CATG_A Cuts at: 2131 2580 6656 8386 2131 Size: 449 4076 1730 2512 Fragments arranged by size: 4076 2512 1730 449 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2368 3047 5186 5382 6122 844 Size: 1524 679 2139 196 740 3489 Fragments arranged by size: 3489 2139 1524 740 679 196 BsrBI CCG'CTC Cuts at: 1394 1823 6654 1394 Size: 429 4831 3507 Fragments arranged by size: 4831 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2470 4478 4666 5409 7262 7436 65 Size: 1183 1222 2008 188 743 1853 174 1396 Fragments arranged by size: 2008 1853 1396 1222 1183 743 188 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4083 7411 7598 1700 Size: 110 2273 3328 187 2869 Fragments arranged by size: 3328 2869 2273 187 110 BsrGI T'GTAC_A Cuts at: 96 2776 96 Size: 2680 6087 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8767 BssSI C'ACGA_G Cuts at: 3577 6827 7839 3577 Size: 3250 1012 4505 Fragments arranged by size: 4505 3250 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4938 794 Size: 4144 4623 BstBI TT'CG_AA Cuts at: 2007 7571 2007 Size: 5564 3203 BstEII G'GTnAC_C Cuts at: 1184 5143 1184 Size: 3959 4808 BstYI r'GATC_y Cuts at: 1972 2254 4033 4273 5029 5610 5830 6097 6464 Size: 282 1779 240 756 581 220 267 367 Cuts at: 6464 6849 6866 8318 8404 8416 8762 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1779 1452 756 581 385 367 346 282 267 240 220 86 17 12 Bsu36I CC'TnA_GG Cuts at: 5615 5615 Size: 8767 BtgI C'CryG_G Cuts at: 513 1065 4719 513 Size: 552 3654 4561 Fragments arranged by size: 4561 3654 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8028 Fragments arranged by size: 8028 683 56 BtsI GCAGTG_nn' Cuts at: 3406 3652 3775 4939 6283 7077 7097 3406 Size: 246 123 1164 1344 794 20 5076 Fragments arranged by size: 5076 1344 1164 794 246 123 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8732 DraI TTT'AAA Cuts at: 6071 6071 Size: 8767 DraIII CAC_nnn'GTG Cuts at: 2049 2049 Size: 8767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3016 7774 809 Size: 507 1700 4758 1802 Fragments arranged by size: 4758 1802 1700 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3432 6214 7103 Size: 54 1425 50 275 18 1602 2782 889 Cuts at: 7103 8732 8 Size: 1629 43 Fragments arranged by size: 2782 1629 1602 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6214 1537 Size: 275 4402 4090 Fragments arranged by size: 4402 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 4163 4321 5648 6697 1353 Size: 795 2015 158 1327 1049 3423 Fragments arranged by size: 3423 2015 1327 1049 795 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2584 7354 7728 7874 387 Size: 1064 138 995 4770 374 146 1280 Fragments arranged by size: 4770 1280 1064 995 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2504 3030 4193 4289 4848 4882 Size: 393 591 281 526 1163 96 559 34 Cuts at: 4882 5855 6830 7427 8214 8482 1239 Size: 973 975 597 787 268 1524 Fragments arranged by size: 1524 1163 975 973 787 597 591 559 526 393 281 268 96 34 EcoNI CCTnn'n_nnAGG Cuts at: 2519 3628 4751 2519 Size: 1109 1123 6535 Fragments arranged by size: 6535 1123 1109 EcoO109I rG'GnC_Cy Cuts at: 2607 5533 5689 2607 Size: 2926 156 5685 Fragments arranged by size: 5685 2926 156 EcoRI G'AATT_C Cuts at: 3673 6076 6454 3673 Size: 2403 378 5986 Fragments arranged by size: 5986 2403 378 EcoRV GAT'ATC Cuts at: 4759 4759 Size: 8767 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 4459 4763 4903 2638 Size: 1821 304 140 6502 Fragments arranged by size: 6502 1821 304 140 FspI TGC'GCA Cuts at: 7273 7273 Size: 8767 HaeII r_GCGC'y Cuts at: 1678 2938 7914 1678 Size: 1260 4976 2531 Fragments arranged by size: 4976 2531 1260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2773 2798 2805 2830 3291 Size: 32 135 32 1201 25 7 25 461 Cuts at: 3291 3323 3571 3603 4169 4201 4346 4378 5137 Size: 32 248 32 566 32 145 32 759 Cuts at: 5137 5169 7408 7440 7482 7514 1373 Size: 32 2239 32 42 32 2626 Fragments arranged by size: 2626 2239 1201 759 566 461 248 145 135 42 32 32 32 32 32 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 3868 5901 6111 6357 669 Size: 988 281 1930 2033 210 246 3079 Fragments arranged by size: 3079 2033 1930 988 281 246 210 HindIII A'AGCT_T Cuts at: 748 5847 748 Size: 5099 3668 HpaI GTT'AAC Cuts at: 3868 6357 3868 Size: 2489 6278 KpnI G_GTAC'C Cuts at: 6092 6092 Size: 8767 MfeI C'AATT_G Cuts at: 6366 6366 Size: 8767 MluI A'CGCG_T Cuts at: 8643 8643 Size: 8767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2934 4030 4397 4680 7881 8065 1305 Size: 1629 1096 367 283 3201 184 2007 Fragments arranged by size: 3201 2007 1629 1096 367 283 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3434 10 Size: 54 1425 343 1602 5343 Fragments arranged by size: 5343 1602 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2136 2339 3413 3428 5009 5570 5594 Size: 648 970 203 1074 15 1581 561 24 Cuts at: 5594 5735 5924 6725 7084 7243 518 Size: 141 189 801 359 159 2042 Fragments arranged by size: 2042 1581 1074 970 801 648 561 359 203 189 159 141 24 15 MspA1I CmG'CkG Cuts at: 1606 2147 3715 6183 6860 8008 8253 1606 Size: 541 1568 2468 677 1148 245 2120 Fragments arranged by size: 2468 2120 1568 1148 677 541 245 NcoI C'CATG_G Cuts at: 513 1065 4719 513 Size: 552 3654 4561 Fragments arranged by size: 4561 3654 552 NdeI CA'TA_TG Cuts at: 387 2716 3831 387 Size: 2329 1115 5323 Fragments arranged by size: 5323 2329 1115 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8767 NotI GC'GGCC_GC Cuts at: 6214 6214 Size: 8767 NsiI A_TGCA'T Cuts at: 2245 3500 2245 Size: 1255 7512 NspI r_CATG'y Cuts at: 1132 1229 4029 6125 7670 1132 Size: 97 2800 2096 1545 2229 Fragments arranged by size: 2800 2229 2096 1545 97 PasI CC'CwG_GG Cuts at: 1348 2462 5744 5908 1348 Size: 1114 3282 164 4207 Fragments arranged by size: 4207 3282 1114 164 PciI A'CATG_T Cuts at: 7666 7666 Size: 8767 PflMI CCAn_nnn'nTGG Cuts at: 1108 2297 3139 3636 5220 1108 Size: 1189 842 497 1584 4655 Fragments arranged by size: 4655 1584 1189 842 497 PfoI T'CCnGG_A Cuts at: 2250 3259 4761 5055 5244 2250 Size: 1009 1502 294 189 5773 Fragments arranged by size: 5773 1502 1009 294 189 PmeI GTTT'AAAC Cuts at: 6071 6071 Size: 8767 PmlI CAC'GTG Cuts at: 4405 4405 Size: 8767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2576 2608 6836 6868 8349 8381 2576 Size: 32 4228 32 1481 32 2962 Fragments arranged by size: 4228 2962 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2607 5689 2607 Size: 3082 5685 PshAI GACnn'nnGTC Cuts at: 1613 2348 1613 Size: 735 8032 PsiI TTA'TAA Cuts at: 6337 6337 Size: 8767 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2084 2116 2760 2792 2084 Size: 32 644 32 8059 Fragments arranged by size: 8059 644 32 32 PstI C_TGCA'G Cuts at: 830 2357 3200 3296 6119 830 Size: 1527 843 96 2823 3478 Fragments arranged by size: 3478 2823 1527 843 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8767 PvuII CAG'CTG Cuts at: 2147 2147 Size: 8767 SalI G'TCGA_C Cuts at: 1936 6109 1936 Size: 4173 4594 SapI GCTCTTCn'nnn_ Cuts at: 4321 5648 4321 Size: 1327 7440 SbfI CC_TGCA'GG Cuts at: 6119 6119 Size: 8767 ScaI AGT'ACT Cuts at: 3609 7015 8727 3609 Size: 3406 1712 3649 Fragments arranged by size: 3649 3406 1712 SexAI A'CCwGG_T Cuts at: 5219 5219 Size: 8767 SfcI C'TryA_G Cuts at: 826 1045 2353 2602 3196 3292 6115 7250 7931 Size: 219 1308 249 594 96 2823 1135 681 Cuts at: 7931 8122 826 Size: 191 1471 Fragments arranged by size: 2823 1471 1308 1135 681 594 249 219 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8767 SmaI CCC'GGG Cuts at: 1872 5480 6094 1872 Size: 3608 614 4545 Fragments arranged by size: 4545 3608 614 SmlI C'TyrA_G Cuts at: 820 1017 1943 2744 3308 5095 6871 7772 8034 Size: 197 926 801 564 1787 1776 901 262 Cuts at: 8034 820 Size: 1553 Fragments arranged by size: 1787 1776 1553 926 901 801 564 262 197 SnaBI TAC'GTA Cuts at: 493 6064 6558 493 Size: 5571 494 2702 Fragments arranged by size: 5571 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8767 SphI G_CATG'C Cuts at: 1132 6125 1132 Size: 4993 3774 SspI AAT'ATT Cuts at: 5 52 2221 3256 6691 5 Size: 47 2169 1035 3435 2081 Fragments arranged by size: 3435 2169 2081 1035 47 StuI AGG'CCT Cuts at: 1517 5620 1517 Size: 4103 4664 StyI C'CwwG_G Cuts at: 513 1065 2033 4429 4719 5529 6206 513 Size: 552 968 2396 290 810 677 3074 Fragments arranged by size: 3074 2396 968 810 677 552 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2391 2633 4623 4926 5440 5486 6794 Size: 1255 811 242 1990 303 514 46 1308 Cuts at: 6794 6811 6964 7149 7594 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1990 1498 1308 1255 811 514 445 303 242 185 153 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2776 3607 7013 Size: 274 80 33 51 1443 799 831 3406 Cuts at: 7013 8725 96 Size: 1712 138 Fragments arranged by size: 3406 1712 1443 831 799 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2176 2208 8727 8759 2176 Size: 32 6519 32 2184 Fragments arranged by size: 6519 2184 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2885 4967 1316 Size: 1569 2082 5116 Fragments arranged by size: 5116 2082 1569 XbaI T'CTAG_A Cuts at: 4684 6103 4684 Size: 1419 7348 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3136 5098 5536 5593 6022 3136 Size: 1962 438 57 429 5881 Fragments arranged by size: 5881 1962 438 429 57 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8767 XmaI C'CCGG_G Cuts at: 1870 5478 6092 1870 Size: 3608 614 4545 Fragments arranged by size: 4545 3608 614 XmnI GAAnn'nnTTC Cuts at: 6896 6896 Size: 8767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2452 3700 6575 276 Size: 53 83 186 936 918 1248 2875 2468 Fragments arranged by size: 2875 2468 1248 936 918 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI