Restriction map for
pFN21ASDA0758
(Linear) (Six-base) MAPSORT of: KIAA0758.seq Check: 4449 from: 1 to: 4038
KIAA0758.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1024 3163 4038
Size: 1024 2139 875
Fragments arranged by size:
2139 1024 875
AatII G_ACGT'C
Cuts at: 0 431 1679 4038
Size: 431 1248 2359
Fragments arranged by size:
2359 1248 431
AccI GT'mk_AC
Cuts at: 0 85 4038
Size: 85 3953
AclI AA'CG_TT
Cuts at: 0 2072 4038
Size: 2072 1966
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 481 2266 2825 2859 3832 4038
Size: 200 281 1785 559 34 973 206
Fragments arranged by size:
1785 973 559 281 206 200 34
AleI CACnn'nnGTG
Cuts at: 0 3571 3712 3901 4038
Size: 3571 141 189 137
Fragments arranged by size:
3571 189 141 137
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 553 585 595 627 4038
Size: 553 32 10 32 3411
Fragments arranged by size:
3411 553 32 32 10
ApoI r'AATT_y
Cuts at: 0 629 1112 1650 2361 4038
Size: 629 483 538 711 1677
Fragments arranged by size:
1677 711 629 538 483
AvaI C'yCGr_G
Cuts at: 0 386 3455 4038
Size: 386 3069 583
Fragments arranged by size:
3069 583 386
BamHI G'GATC_C
Cuts at: 0 231 2010 4038
Size: 231 1779 2028
Fragments arranged by size:
2028 1779 231
BanI G'GyrC_C
Cuts at: 0 443 4038
Size: 443 3595
BanII G_rGCy'C
Cuts at: 0 1963 2215 4038
Size: 1963 252 1823
Fragments arranged by size:
1963 1823 252
BbsI GAAGACnn'nnnn_
Cuts at: 0 97 391 423 557 2856 3291 3759 3851
Size: 97 294 32 134 2299 435 468 92
Cuts at: 3851 4038
Size: 187
Fragments arranged by size:
2299 468 435 294 187 134 97 92
32
BbvCI CC'TCA_GC
Cuts at: 0 3292 4038
Size: 3292 746
BclI T'GATC_A
Cuts at: 0 2204 3539 4038
Size: 2204 1335 499
Fragments arranged by size:
2204 1335 499
BfrBI ATG'CAT
Cuts at: 0 220 1475 4038
Size: 220 1255 2563
Fragments arranged by size:
2563 1255 220
BglI GCCn_nnn'nGGC
Cuts at: 0 2843 3259 3517 4038
Size: 2843 416 258 521
Fragments arranged by size:
2843 521 416 258
Bme1580I G_kGCm'C
Cuts at: 0 446 1309 4038
Size: 446 863 2729
Fragments arranged by size:
2729 863 446
BmrI ACTGGGnnnn_n'
Cuts at: 0 1296 2127 3328 3504 4038
Size: 1296 831 1201 176 534
Fragments arranged by size:
1296 1201 831 534 176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2234 4038
Size: 2234 1804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1007 2170 4038
Size: 1007 1163 1868
Fragments arranged by size:
1868 1163 1007
Bpu10I CC'TnA_GC
Cuts at: 0 2195 2729 3292 4038
Size: 2195 534 563 746
Fragments arranged by size:
2195 746 563 534
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1270 3093 4038
Size: 742 528 1823 945
Fragments arranged by size:
1823 945 742 528
BsaI GGTCTCn'nnnn_
Cuts at: 0 3709 4038
Size: 3709 329
BsaAI yAC'GTr
Cuts at: 0 2382 4038
Size: 2382 1656
BsaHI Gr'CG_yC
Cuts at: 0 428 1676 4038
Size: 428 1248 2362
Fragments arranged by size:
2362 1248 428
BsaWI w'CCGG_w
Cuts at: 0 614 4038
Size: 614 3424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 553 583 775 805 4038
Size: 553 30 192 30 3233
Fragments arranged by size:
3233 553 192 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1572 1792 2155 4038
Size: 1572 220 363 1883
Fragments arranged by size:
1883 1572 363 220
BseYI C'CCAG_C
Cuts at: 0 2077 3438 3449 3531 4038
Size: 2077 1361 11 82 507
Fragments arranged by size:
2077 1361 507 82 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 2572 4038
Size: 47 2525 1466
Fragments arranged by size:
2525 1466 47
BsiHKAI G_wGCw'C
Cuts at: 0 361 2387 4038
Size: 361 2026 1651
Fragments arranged by size:
2026 1651 361
Bsp1286I G_dGCh'C
Cuts at: 0 361 446 1309 1963 2215 2387 4038
Size: 361 85 863 654 252 172 1651
Fragments arranged by size:
1651 863 654 361 252 172 85
BspEI T'CCGG_A
Cuts at: 0 614 4038
Size: 614 3424
BspHI T'CATG_A
Cuts at: 0 108 557 4038
Size: 108 449 3481
Fragments arranged by size:
3481 449 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 345 1024 3163 3359 4038
Size: 345 679 2139 196 679
Fragments arranged by size:
2139 679 679 345 196
BsrDI GCAATG_nn'
Cuts at: 0 447 2455 2643 3386 4038
Size: 447 2008 188 743 652
Fragments arranged by size:
2008 743 652 447 188
BsrFI r'CCGG_y
Cuts at: 0 2060 4038
Size: 2060 1978
BsrGI T'GTAC_A
Cuts at: 0 753 4038
Size: 753 3285
BssSI C'ACGA_G
Cuts at: 0 1554 4038
Size: 1554 2484
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2915 4038
Size: 2915 1123
BstEII G'GTnAC_C
Cuts at: 0 3120 4038
Size: 3120 918
BstYI r'GATC_y
Cuts at: 0 231 2010 2250 3006 3587 3807 4038
Size: 231 1779 240 756 581 220 231
Fragments arranged by size:
1779 756 581 240 231 231 220
Bsu36I CC'TnA_GG
Cuts at: 0 3592 4038
Size: 3592 446
BtgI C'CryG_G
Cuts at: 0 2696 4038
Size: 2696 1342
BtsI GCAGTG_nn'
Cuts at: 0 1383 1629 1752 2916 4038
Size: 1383 246 123 1164 1122
Fragments arranged by size:
1383 1164 1122 246 123
DraIII CAC_nnn'GTG
Cuts at: 0 26 4038
Size: 26 4012
DrdI GACnn_nn'nnGTC
Cuts at: 0 993 4038
Size: 993 3045
EaeI y'GGCC_r
Cuts at: 0 1409 4038
Size: 1409 2629
EarI CTCTTCn'nnn_
Cuts at: 0 125 2140 2298 3625 4038
Size: 125 2015 158 1327 413
Fragments arranged by size:
2015 1327 413 158 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 561 4038
Size: 561 3477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 481 1007 2170 2266 2825 2859 3832
Size: 200 281 526 1163 96 559 34 973
Cuts at: 3832 4038
Size: 206
Fragments arranged by size:
1163 973 559 526 281 206 200 96
34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 496 1605 2728 4038
Size: 496 1109 1123 1310
Fragments arranged by size:
1310 1123 1109 496
EcoO109I rG'GnC_Cy
Cuts at: 0 584 3510 3666 4038
Size: 584 2926 156 372
Fragments arranged by size:
2926 584 372 156
EcoRI G'AATT_C
Cuts at: 0 1650 4038
Size: 1650 2388
EcoRV GAT'ATC
Cuts at: 0 2736 4038
Size: 2736 1302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 615 2436 2740 2880 4038
Size: 615 1821 304 140 1158
Fragments arranged by size:
1821 1158 615 304 140
HaeII r_GCGC'y
Cuts at: 0 915 4038
Size: 915 3123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 750 775 782 807 1268 1300 1548 1580
Size: 750 25 7 25 461 32 248 32
Cuts at: 1580 2146 2178 2323 2355 3114 3146 4038
Size: 566 32 145 32 759 32 892
Fragments arranged by size:
892 759 750 566 461 248 145 32
32 32 32 32 25 25 7
HincII GTy'rAC
Cuts at: 0 1845 3878 4038
Size: 1845 2033 160
Fragments arranged by size:
2033 1845 160
HindIII A'AGCT_T
Cuts at: 0 3824 4038
Size: 3824 214
HpaI GTT'AAC
Cuts at: 0 1845 4038
Size: 1845 2193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 2007 2374 2657 4038
Size: 911 1096 367 283 1381
Fragments arranged by size:
1381 1096 911 367 283
MscI TGG'CCA
Cuts at: 0 1411 4038
Size: 1411 2627
MslI CAynn'nnrTG
Cuts at: 0 113 316 1390 1405 2986 3547 3571 3712
Size: 113 203 1074 15 1581 561 24 141
Cuts at: 3712 3901 4038
Size: 189 137
Fragments arranged by size:
1581 1074 561 203 189 141 137 113
24 15
MspA1I CmG'CkG
Cuts at: 0 124 1692 4038
Size: 124 1568 2346
Fragments arranged by size:
2346 1568 124
NcoI C'CATG_G
Cuts at: 0 2696 4038
Size: 2696 1342
NdeI CA'TA_TG
Cuts at: 0 693 1808 4038
Size: 693 1115 2230
Fragments arranged by size:
2230 1115 693
NsiI A_TGCA'T
Cuts at: 0 222 1477 4038
Size: 222 1255 2561
Fragments arranged by size:
2561 1255 222
NspI r_CATG'y
Cuts at: 0 2006 4038
Size: 2006 2032
PasI CC'CwG_GG
Cuts at: 0 439 3721 3885 4038
Size: 439 3282 164 153
Fragments arranged by size:
3282 439 164 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 274 1116 1613 3197 4038
Size: 274 842 497 1584 841
Fragments arranged by size:
1584 842 841 497 274
PfoI T'CCnGG_A
Cuts at: 0 227 1236 2738 3032 3221 4038
Size: 227 1009 1502 294 189 817
Fragments arranged by size:
1502 1009 817 294 227 189
PmlI CAC'GTG
Cuts at: 0 2382 4038
Size: 2382 1656
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 553 585 4038
Size: 553 32 3453
Fragments arranged by size:
3453 553 32
PpuMI rG'GwC_Cy
Cuts at: 0 584 3666 4038
Size: 584 3082 372
Fragments arranged by size:
3082 584 372
PshAI GACnn'nnGTC
Cuts at: 0 325 4038
Size: 325 3713
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 61 93 737 769 4038
Size: 61 32 644 32 3269
Fragments arranged by size:
3269 644 61 32 32
PstI C_TGCA'G
Cuts at: 0 334 1177 1273 4038
Size: 334 843 96 2765
Fragments arranged by size:
2765 843 334 96
PvuII CAG'CTG
Cuts at: 0 124 4038
Size: 124 3914
SapI GCTCTTCn'nnn_
Cuts at: 0 2298 3625 4038
Size: 2298 1327 413
Fragments arranged by size:
2298 1327 413
ScaI AGT'ACT
Cuts at: 0 1586 4038
Size: 1586 2452
SexAI A'CCwGG_T
Cuts at: 0 3196 4038
Size: 3196 842
SfcI C'TryA_G
Cuts at: 0 330 579 1173 1269 4038
Size: 330 249 594 96 2769
Fragments arranged by size:
2769 594 330 249 96
SmaI CCC'GGG
Cuts at: 0 3457 4038
Size: 3457 581
SmlI C'TyrA_G
Cuts at: 0 721 1285 3072 4038
Size: 721 564 1787 966
Fragments arranged by size:
1787 966 721 564
SspI AAT'ATT
Cuts at: 0 198 1233 4038
Size: 198 1035 2805
Fragments arranged by size:
2805 1035 198
StuI AGG'CCT
Cuts at: 0 3597 4038
Size: 3597 441
StyI C'CwwG_G
Cuts at: 0 10 2406 2696 3506 4038
Size: 10 2396 290 810 532
Fragments arranged by size:
2396 810 532 290 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 368 610 2600 2903 3417 3463 4038
Size: 368 242 1990 303 514 46 575
Fragments arranged by size:
1990 575 514 368 303 242 46
TatI w'GTAC_w
Cuts at: 0 753 1584 4038
Size: 753 831 2454
Fragments arranged by size:
2454 831 753
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 153 185 4038
Size: 153 32 3853
Fragments arranged by size:
3853 153 32
Tth111I GACn'n_nGTC
Cuts at: 0 862 2944 4038
Size: 862 2082 1094
Fragments arranged by size:
2082 1094 862
XbaI T'CTAG_A
Cuts at: 0 2661 4038
Size: 2661 1377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1113 3075 3513 3570 3999 4038
Size: 1113 1962 438 57 429 39
Fragments arranged by size:
1962 1113 438 429 57 39
XmaI C'CCGG_G
Cuts at: 0 3455 4038
Size: 3455 583
ZraI GAC'GTC
Cuts at: 0 429 1677 4038
Size: 429 1248 2361
Fragments arranged by size:
2361 1248 429
Enzymes that do cut:
AarI AatII AccI AclI AcuI AleI AloI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BclI BfrBI
BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII HpaI MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PsrI PstI PvuII
SapI ScaI SexAI SfcI SmaI SmlI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AhdI AlfI AlwNI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BciVI BglII BlpI BmgBI BmtI BsaBI BsiEI
BsiWI BsmI BsmBI BsrBI BssHII BstBI BstXI BstZ17I
BtgZI ClaI CspCI DraI EagI EcoICRI FseI FspI
FspAI KasI KpnI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PciI PmeI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SanDI SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SwaI XhoI XmnI