Restriction map for
pFN21ASDA0757
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI AspEI AspI
AvaII AviII AvrII BbrPI BcgI
BclI BfrI BglII BlnI BsaBI
BsaWI BseAI BsiWI BsmI BspEI
BspLU11I BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I CpoI DraI DrdI
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MamI MfeI MluI MroI
MseI MslI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tth111I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 976 GGTACC G^GTACC
AccI 369 GTCGAC GT^[AC][GT]AC
ApaI 345 GGGCCC GGGCC^C
Asp700I 315 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 976 GGTACC G^GTACC
AvaI 750 CTCGAG C^[CT]CG[GA]G
BamHI 165 GGATCC G^GATCC
BbsI 818 GTCTTC GAAGAC(2/6)
BbvCI 1449 CCTCAGC CCTCAGC(-5/-2)
BglI 1270 GCCCACGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 818 GTCTTC GAAGAC(2/6)
BsaAI 55 TACGTG [CT]AC^GT[GA]
BsmBI 277 GAGACG CGTCTC(1/5)
BsmFI 851 GGGAC GGGAC(10/14)
BsoBI 750 CTCGAG C^[CT]CG[GA]G
BspDI 176 ATCGAT AT^CGAT
BspHI 1257 TCATGA T^CATGA
BspMI 1411 ACCTGC ACCTGC(4/8)
BsrBI 706 GAGCGG CCGCTC(-3/-3)
BstXI 11 CCAGTTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 176 ATCGAT AT^CGAT
EagI 1060 CGGCCG C^GGCCG
EclXI 1060 CGGCCG C^GGCCG
Eco52I 1060 CGGCCG C^GGCCG
EcoNI 153 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 319 GAATTC G^AATTC
HincII 369 GTCGAC GT[CT]^[GA]AC
HindII 369 GTCGAC GT[CT]^[GA]AC
KpnI 976 GGTACC GGTAC^C
NcoI 899 CCATGG C^CATGG
NspI 269 GCATGC [GA]CATG^[CT]
PaeR7I 750 CTCGAG C^TCGAG
PstI 1167 CTGCAG CTGCA^G
PvuII 1516 CAGCTG CAG^CTG
RcaI 1257 TCATGA T^CATGA
SalI 369 GTCGAC G^TCGAC
SfcI 1167 CTGCAG C^T[GA][CT]AG
SphI 269 GCATGC GCATG^C
TfiI 179 GATTC G^A[AT]TC
XcmI 671 CCATGTCACGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 750 CTCGAG C^TCGAG
XmnI 315 GAAGGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 28 AAATTC [GA]^AATT[CT]
319 GAATTC
AlwNI 774 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1016 CAGACTCTG
ApoI 28 AAATTC [GA]^AATT[CT]
319 GAATTC
BalI 503 TGGCCA TGG^CCA
902 TGGCCA
BbeI 259 GGCGCC GGCGC^C
1399 GGCGCC
BciVI 21 GGATAC GTATCC(6/5)
81 GGATAC
BfaI 239 CTAG C^TAG
529 CTAG
BlpI 147 GCTCAGC GC^T[ACGT]AGC
228 GCTCAGC
Bpu1102I 147 GCTCAGC GC^T[ACGT]AGC
228 GCTCAGC
BsaI 463 GAGACC GGTCTC(1/5)
483 GGTCTC
BseRI 234 CTCCTC GAGGAG(10/8)
1173 CTCCTC
BsiEI 1060 CGGCCG CG[GA][CT]^CG
1320 CGACCG
Bsp1407I 1010 TGTACA T^GTACA
1553 TGTACA
BsrGI 1010 TGTACA T^GTACA
1553 TGTACA
BstYI 165 GGATCC [GA]^GATC[CT]
1005 GGATCT
CelII 147 GCTCAGC GC^T[ACGT]AGC
228 GCTCAGC
DraII 345 GGGCCCT [GA]G^G[ACGT]CC[CT]
1313 AGGGCCT
DraIII 157 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1416 CACCCAGTG
EarI 295 CTCTTC CTCTTC(1/4)
382 GAAGAG
EcoO109I 345 GGGCCCT [GA]G^G[ACGT]CC[CT]
1313 AGGGCCT
EcoT14I 716 CCAAGG C^C[AT][AT]GG
899 CCATGG
HgaI 625 GACGC GACGC(5/10)
1441 GACGC
HphI 474 GGTGA GGTGA(8/7)
1205 TCACC
KasI 259 GGCGCC G^GCGCC
1399 GGCGCC
Ksp632I 295 CTCTTC CTCTTC(1/4)
382 GAAGAG
MaeI 239 CTAG C^TAG
529 CTAG
MaeII 56 ACGT A^CGT
1001 ACGT
MflI 165 GGATCC [GA]^GATC[CT]
1005 GGATCT
MluNI 503 TGGCCA TGG^CCA
902 TGGCCA
MscI 503 TGGCCA TGG^CCA
902 TGGCCA
NarI 259 GGCGCC GG^CGCC
1399 GGCGCC
SfoI 259 GGCGCC GGC^GCC
1399 GGCGCC
SmlI 750 CTCGAG C^T[CT][GA]AG
859 CTCAAG
SspBI 1010 TGTACA T^GTACA
1553 TGTACA
StuI 806 AGGCCT AGG^CCT
1247 AGGCCT
StyI 716 CCAAGG C^C[AT][AT]GG
899 CCATGG
Tsp509I 29 AATT ^AATT
320 AATT
XhoII 165 GGATCC [GA]^GATC[CT]
1005 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI Aor51HI AspHI BanI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
Eco47III EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI Tsp45I TspRI TthHB8I