Restriction map for
pFN21ASDA0757
(Linear) (Six-base) MAPSORT of: KIAA0757.seq Check: 7168 from: 1 to: 1560
KIAA0757.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1420 1560
Size: 1420 140
AccI GT'mk_AC
Cuts at: 0 370 1560
Size: 370 1190
Acc65I G'GTAC_C
Cuts at: 0 976 1560
Size: 976 584
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 823 1560
Size: 249 574 737
Fragments arranged by size:
737 574 249
AfeI AGC'GCT
Cuts at: 0 1111 1189 1455 1560
Size: 1111 78 266 105
Fragments arranged by size:
1111 266 105 78
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 29 1513 1545 1560
Size: 29 1484 32 15
Fragments arranged by size:
1484 32 29 15
AlwNI CAG_nnn'CTG
Cuts at: 0 779 1021 1560
Size: 779 242 539
Fragments arranged by size:
779 539 242
ApaI G_GGCC'C
Cuts at: 0 349 1560
Size: 349 1211
ApoI r'AATT_y
Cuts at: 0 28 319 1560
Size: 28 291 1241
Fragments arranged by size:
1241 291 28
AvaI C'yCGr_G
Cuts at: 0 750 1560
Size: 750 810
BamHI G'GATC_C
Cuts at: 0 165 1560
Size: 165 1395
BanI G'GyrC_C
Cuts at: 0 259 976 1399 1560
Size: 259 717 423 161
Fragments arranged by size:
717 423 259 161
BanII G_rGCy'C
Cuts at: 0 217 252 349 458 497 1560
Size: 217 35 97 109 39 1063
Fragments arranged by size:
1063 217 109 97 39 35
BbeI G_GCGC'C
Cuts at: 0 263 1403 1560
Size: 263 1140 157
Fragments arranged by size:
1140 263 157
BbsI GAAGACnn'nnnn_
Cuts at: 0 811 1560
Size: 811 749
BbvCI CC'TCA_GC
Cuts at: 0 1450 1560
Size: 1450 110
BciVI GTATCCnnnnn_n'
Cuts at: 0 15 75 1560
Size: 15 60 1485
Fragments arranged by size:
1485 60 15
BglI GCCn_nnn'nGGC
Cuts at: 0 1276 1560
Size: 1276 284
BlpI GC'TnA_GC
Cuts at: 0 148 229 1560
Size: 148 81 1331
Fragments arranged by size:
1331 148 81
Bme1580I G_kGCm'C
Cuts at: 0 349 436 1560
Size: 349 87 1124
Fragments arranged by size:
1124 349 87
BmgBI CAC'GTC
Cuts at: 0 1002 1560
Size: 1002 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 5 789 1413 1560
Size: 5 784 624 147
Fragments arranged by size:
784 624 147 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 703 1560
Size: 703 857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 454 673 1199 1560
Size: 295 159 219 526 361
Fragments arranged by size:
526 361 295 219 159
Bpu10I CC'TnA_GC
Cuts at: 0 403 425 1450 1560
Size: 403 22 1025 110
Fragments arranged by size:
1025 403 110 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1560
Size: 844 716
BsaI GGTCTCn'nnnn_
Cuts at: 0 457 489 1560
Size: 457 32 1071
Fragments arranged by size:
1071 457 32
BsaAI yAC'GTr
Cuts at: 0 57 1560
Size: 57 1503
BsaHI Gr'CG_yC
Cuts at: 0 260 626 1400 1442 1560
Size: 260 366 774 42 118
Fragments arranged by size:
774 366 260 118 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1197 1227 1324 1354 1560
Size: 1197 30 97 30 206
Fragments arranged by size:
1197 206 97 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 1164 1560
Size: 225 939 396
Fragments arranged by size:
939 396 225
BseYI C'CCAG_C
Cuts at: 0 429 949 1228 1240 1535 1560
Size: 429 520 279 12 295 25
Fragments arranged by size:
520 429 295 279 25 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 139 238 620 1398 1477 1560
Size: 79 60 99 382 778 79 83
Fragments arranged by size:
778 382 99 83 79 79 60
BsiEI CG_ry'CG
Cuts at: 0 1063 1323 1560
Size: 1063 260 237
Fragments arranged by size:
1063 260 237
BsiHKAI G_wGCw'C
Cuts at: 0 217 458 497 659 1560
Size: 217 241 39 162 901
Fragments arranged by size:
901 241 217 162 39
BsmBI CGTCTCn'nnnn_
Cuts at: 0 271 1560
Size: 271 1289
Bsp1286I G_dGCh'C
Cuts at: 0 217 252 349 436 458 497 659 1560
Size: 217 35 97 87 22 39 162 901
Fragments arranged by size:
901 217 162 97 87 39 35 22
BspHI T'CATG_A
Cuts at: 0 1257 1560
Size: 1257 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1420 1560
Size: 1420 140
BsrBI CCG'CTC
Cuts at: 0 708 1560
Size: 708 852
BsrGI T'GTAC_A
Cuts at: 0 1010 1553 1560
Size: 1010 543 7
Fragments arranged by size:
1010 543 7
BssSI C'ACGA_G
Cuts at: 0 457 677 1549 1560
Size: 457 220 872 11
Fragments arranged by size:
872 457 220 11
BstXI CCAn_nnnn'nTGG
Cuts at: 0 18 1560
Size: 18 1542
BstYI r'GATC_y
Cuts at: 0 165 1005 1560
Size: 165 840 555
Fragments arranged by size:
840 555 165
BtgI C'CryG_G
Cuts at: 0 307 899 915 1272 1560
Size: 307 592 16 357 288
Fragments arranged by size:
592 357 307 288 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 128 1560
Size: 128 1432
BtsI GCAGTG_nn'
Cuts at: 0 434 603 1560
Size: 434 169 957
Fragments arranged by size:
957 434 169
ClaI AT'CG_AT
Cuts at: 0 177 1560
Size: 177 1383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 827 862 1560
Size: 827 35 698
Fragments arranged by size:
827 698 35
DraIII CAC_nnn'GTG
Cuts at: 0 162 1421 1560
Size: 162 1259 139
Fragments arranged by size:
1259 162 139
EaeI y'GGCC_r
Cuts at: 0 503 698 713 902 1060 1560
Size: 503 195 15 189 158 500
Fragments arranged by size:
503 500 195 189 158 15
EagI C'GGCC_G
Cuts at: 0 1060 1560
Size: 1060 500
EarI CTCTTCn'nnn_
Cuts at: 0 301 377 1560
Size: 301 76 1183
Fragments arranged by size:
1183 301 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 865 1122 1560
Size: 865 257 438
Fragments arranged by size:
865 438 257
EcoICRI GAG'CTC
Cuts at: 0 215 456 495 1560
Size: 215 241 39 1065
Fragments arranged by size:
1065 241 215 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 295 454 673 823 1199 1560
Size: 249 46 159 219 150 376 361
Fragments arranged by size:
376 361 249 219 159 150 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 157 1560
Size: 157 1403
EcoO109I rG'GnC_Cy
Cuts at: 0 346 1314 1560
Size: 346 968 246
Fragments arranged by size:
968 346 246
EcoRI G'AATT_C
Cuts at: 0 319 1560
Size: 319 1241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 57 1560
Size: 57 1503
HaeII r_GCGC'y
Cuts at: 0 263 1113 1191 1235 1403 1457 1560
Size: 263 850 78 44 168 54 103
Fragments arranged by size:
850 263 168 103 78 54 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1078 1110 1560
Size: 1078 32 450
Fragments arranged by size:
1078 450 32
HincII GTy'rAC
Cuts at: 0 371 1560
Size: 371 1189
KasI G'GCGC_C
Cuts at: 0 259 1399 1560
Size: 259 1140 161
Fragments arranged by size:
1140 259 161
KpnI G_GTAC'C
Cuts at: 0 980 1560
Size: 980 580
MscI TGG'CCA
Cuts at: 0 505 904 1560
Size: 505 399 656
Fragments arranged by size:
656 505 399
MspA1I CmG'CkG
Cuts at: 0 102 1376 1518 1560
Size: 102 1274 142 42
Fragments arranged by size:
1274 142 102 42
NarI GG'CG_CC
Cuts at: 0 260 1400 1560
Size: 260 1140 160
Fragments arranged by size:
1140 260 160
NcoI C'CATG_G
Cuts at: 0 899 1560
Size: 899 661
NspI r_CATG'y
Cuts at: 0 273 1560
Size: 273 1287
PasI CC'CwG_GG
Cuts at: 0 1028 1560
Size: 1028 532
PspOMI G'GGCC_C
Cuts at: 0 345 1560
Size: 345 1215
PspXI vC'TCGA_Gb
Cuts at: 0 750 1560
Size: 750 810
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 46 78 1560
Size: 46 32 1482
Fragments arranged by size:
1482 46 32
PstI C_TGCA'G
Cuts at: 0 1171 1560
Size: 1171 389
PvuII CAG'CTG
Cuts at: 0 1518 1560
Size: 1518 42
SacI G_AGCT'C
Cuts at: 0 217 458 497 1560
Size: 217 241 39 1063
Fragments arranged by size:
1063 241 217 39
SalI G'TCGA_C
Cuts at: 0 369 1560
Size: 369 1191
SfcI C'TryA_G
Cuts at: 0 1167 1560
Size: 1167 393
SfoI GGC'GCC
Cuts at: 0 261 1401 1560
Size: 261 1140 159
Fragments arranged by size:
1140 261 159
SmlI C'TyrA_G
Cuts at: 0 750 859 1560
Size: 750 109 701
Fragments arranged by size:
750 701 109
SphI G_CATG'C
Cuts at: 0 273 1560
Size: 273 1287
StuI AGG'CCT
Cuts at: 0 808 1249 1560
Size: 808 441 311
Fragments arranged by size:
808 441 311
StyI C'CwwG_G
Cuts at: 0 716 899 1560
Size: 716 183 661
Fragments arranged by size:
716 661 183
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1432 1549 1560
Size: 1432 117 11
Fragments arranged by size:
1432 117 11
TatI w'GTAC_w
Cuts at: 0 1010 1553 1560
Size: 1010 543 7
Fragments arranged by size:
1010 543 7
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1197 1229 1324 1330 1356 1362 1560
Size: 1197 32 95 6 26 6 198
Fragments arranged by size:
1197 198 95 32 26 6 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 1560
Size: 678 882
XhoI C'TCGA_G
Cuts at: 0 750 1560
Size: 750 810
XmnI GAAnn'nnTTC
Cuts at: 0 319 1560
Size: 319 1241
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrGI BssSI BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI
KpnI MscI MspA1I NarI NcoI NspI PasI PspOMI
PspXI PsrI PstI PvuII SacI SalI SfcI SfoI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI
BfrBI BglII BmtI BsaBI BsaWI BsiWI BsmI BspEI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
DraI DrdI EcoRV FseI FspI FspAI HindIII HpaI
MfeI MluI MmeI MslI NaeI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PvuI RsrII SacII
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XmaI ZraI