Restriction map for
pFN21ASDA0753
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII BbrPI BbvCI BclI
BfrI BlpI Bpu1102I BsaAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstUI Bsu36I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV EcoT22I FauI FbaI FseI
FspI HpaI KpnI KspI MluI
MslI MvnI NaeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SwaI Tth111I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 1341 TCCGGA T^CCGGA
AcyI 1527 GGCGCC G[GA]^CG[CT]C
AvrII 43 CCTAGG C^CTAGG
BalI 1213 TGGCCA TGG^CCA
BbeI 1527 GGCGCC GGCGC^C
BglI 928 GCCATTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 43 CCTAGG C^CTAGG
BsaBI 1315 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1527 GGCGCC G[GA]^CG[CT]C
BsaI 1345 GAGACC GGTCTC(1/5)
BsaWI 1341 TCCGGA [AT]^CCGG[AT]
BseAI 1341 TCCGGA T^CCGGA
BsmBI 1700 CGTCTC CGTCTC(1/5)
BsmI 800 GAATGC GAATGC(1/-1)
BspEI 1341 TCCGGA T^CCGGA
BspMI 822 GCAGGT ACCTGC(4/8)
BsrDI 895 GCAATG GCAATG(2/0)
BsrFI 1637 ACCGGC [GA]^CCGG[CT]
Bst1107I 517 GTATAC GTA^TAC
BstEII 613 GGTGACC G^GT[ACGT]ACC
BstPI 613 GGTGACC G^GT[ACGT]ACC
BstXI 51 CCAACTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 517 GTATAC GTA^TAC
Cfr10I 1637 ACCGGC [GA]^CCGG[CT]
DraIII 424 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1213 TGGCCA [CT]^GGCC[GA]
EcoO65I 613 GGTGACC G^GT[ACGT]ACC
EcoRI 2881 GAATTC G^AATTC
HaeII 1527 GGCGCC [GA]GCGC^[CT]
HgaI 1699 GCGTC GACGC(5/10)
Hin1I 1527 GGCGCC G[GA]^CG[CT]C
HincII 1283 GTCGAC GT[CT]^[GA]AC
HindII 1283 GTCGAC GT[CT]^[GA]AC
KasI 1527 GGCGCC G^GCGCC
MamI 1315 GATAAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2002 CAATTG C^AATTG
MluNI 1213 TGGCCA TGG^CCA
MroI 1341 TCCGGA T^CCGGA
MscI 1213 TGGCCA TGG^CCA
MunI 2002 CAATTG C^AATTG
NarI 1527 GGCGCC GG^CGCC
NcoI 1666 CCATGG C^CATGG
NdeI 2399 CATATG CA^TATG
PflMI 423 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 46 AGGACCC [GA]G^G[AT]CC[CT]
PstI 100 CTGCAG CTGCA^G
SalI 1283 GTCGAC G^TCGAC
SapI 2794 GAAGAGC GCTCTTC(1/4)
ScaI 78 AGTACT AGT^ACT
SfoI 1527 GGCGCC GGC^GCC
SmlI 1781 CTCAAG C^T[CT][GA]AG
SphI 1904 GCATGC GCATG^C
SspI 2113 AATATT AAT^ATT
StuI 1199 AGGCCT AGG^CCT
Van91I 423 CCACAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwNI 1802 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
2861 CAGAAGCTG
BamHI 738 GGATCC G^GATCC
2517 GGATCC
BbsI 329 GAAGAC GAAGAC(2/6)
2499 GAAGAC
BciVI 1670 GGATAC GTATCC(6/5)
686 GTATCC
BcnI 586 CCGGG CC^[GC]GG
2677 CCGGG
BpuAI 329 GAAGAC GAAGAC(2/6)
2499 GAAGAC
BsgI 2162 CTGCAC GTGCAG(16/14)
1600 GTGCAG
BspHI 2330 TCATGA T^CATGA
2742 TCATGA
CfoI 1528 GCGC GCG^C
2337 GCGC
HhaI 1528 GCGC GCG^C
2337 GCGC
HinP1I 1528 GCGC G^CGC
2337 GCGC
MaeII 753 ACGT A^CGT
809 ACGT
MspA1I 97 CAGCTG C[AC]G^C[GT]G
2177 CAGCTG
NciI 586 CCGGG CC^[GC]GG
2677 CCGGG
NspI 162 ACATGC [GA]CATG^[CT]
1904 GCATGC
PvuII 97 CAGCTG CAG^CTG
2177 CAGCTG
RcaI 2330 TCATGA T^CATGA
2742 TCATGA
XbaI 1416 TCTAGA T^CTAGA
1945 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanI
BanII BbvI BcgI BfaI BglII
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII