(Circular) (Six-base) MAPSORT of: pFN21ASDA0753.seq Check: 9112 from: 1 to: 7630 pFN21ASDA0753 7630 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5440 278 Size: 53 83 186 936 3904 2468 Fragments arranged by size: 3904 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2541 3307 3592 4973 1937 Size: 604 766 285 1381 4594 Fragments arranged by size: 4594 1381 766 604 285 Acc65I G'GTAC_C Cuts at: 4951 4951 Size: 7630 AclI AA'CG_TT Cuts at: 1563 5757 6130 1563 Size: 4194 373 3063 Fragments arranged by size: 4194 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 2228 2600 4023 4844 5693 7077 7345 Size: 458 138 372 1423 821 849 1384 268 Cuts at: 7345 1632 Size: 1917 Fragments arranged by size: 1917 1423 1384 849 821 458 372 268 138 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7433 AflIII A'CryG_T Cuts at: 1203 6529 7506 1203 Size: 5326 977 1327 Fragments arranged by size: 5326 1327 977 AgeI A'CCGG_T Cuts at: 6461 6461 Size: 7630 AhdI GACnn_n'nnGTC Cuts at: 6359 6359 Size: 7630 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7630 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3348 1184 Size: 2164 5466 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2766 2798 2766 Size: 32 7598 AlwNI CAG_nnn'CTG Cuts at: 3830 4889 5373 6945 3830 Size: 1059 484 1572 4515 Fragments arranged by size: 4515 1572 1059 484 ApaLI G'TGCA_C Cuts at: 5687 6843 5687 Size: 1156 6474 ApoI r'AATT_y Cuts at: 1493 2587 3189 4904 4939 5165 5317 7426 1493 Size: 1094 602 1715 35 226 152 2109 1697 Fragments arranged by size: 2109 1715 1697 1094 602 226 152 35 AseI AT'TA_AT Cuts at: 160 1032 6184 160 Size: 872 5152 1606 Fragments arranged by size: 5152 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7630 AvaI C'yCGr_G Cuts at: 1870 1943 4955 1870 Size: 73 3012 4545 Fragments arranged by size: 4545 3012 73 AvrII C'CTAG_G Cuts at: 2066 2066 Size: 7630 BamHI G'GATC_C Cuts at: 2761 4540 4960 5327 2761 Size: 1779 420 367 5064 Fragments arranged by size: 5064 1779 420 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2531 3550 3685 4951 6406 Size: 325 217 627 744 1019 135 1266 1455 Cuts at: 6406 618 Size: 1842 Fragments arranged by size: 1842 1455 1266 1019 744 627 325 217 135 BanII G_rGCy'C Cuts at: 1391 3996 4269 4684 4949 1391 Size: 2605 273 415 265 4072 Fragments arranged by size: 4072 2605 415 273 265 BbeI G_GCGC'C Cuts at: 3554 3554 Size: 7630 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2359 4529 928 Size: 971 460 2170 4029 Fragments arranged by size: 4029 2170 971 460 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2160 2194 2792 2826 4733 4767 4862 Size: 34 223 34 598 34 1907 34 95 Cuts at: 4862 4896 5821 5855 1903 Size: 34 925 34 3678 Fragments arranged by size: 3678 1907 925 598 223 95 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2720 3687 5522 6732 2720 Size: 967 1835 1210 3618 Fragments arranged by size: 3618 1835 1210 967 BclI T'GATC_A Cuts at: 1548 1548 Size: 7630 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2957 6241 136 Size: 107 122 71 631 605 1285 3284 1525 Fragments arranged by size: 3284 1525 1285 631 605 122 107 71 BglII A'GATC_T Cuts at: 2572 4290 4342 7625 2572 Size: 1718 52 3283 2577 Fragments arranged by size: 3283 2577 1718 52 BlpI GC'TnA_GC Cuts at: 5047 5047 Size: 7630 Bme1580I G_kGCm'C Cuts at: 1790 5691 6847 1790 Size: 3901 1156 2573 Fragments arranged by size: 3901 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7630 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3038 5361 6319 Size: 416 53 83 399 24 1621 2323 958 Cuts at: 6319 442 Size: 1753 Fragments arranged by size: 2323 1753 1621 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7630 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4703 4703 Size: 7630 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2506 2672 2822 3773 4367 6290 1239 Size: 1267 166 150 951 594 1923 2579 Fragments arranged by size: 2579 1923 1267 951 594 166 150 Bpu10I CC'TnA_GC Cuts at: 4309 4309 Size: 7630 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3789 5755 6620 6918 3789 Size: 1966 865 298 4501 Fragments arranged by size: 4501 1966 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3362 6293 882 Size: 617 1863 2931 2219 Fragments arranged by size: 2931 2219 1863 617 BsaAI yAC'GTr Cuts at: 493 1204 4927 5421 493 Size: 711 3723 494 2702 Fragments arranged by size: 3723 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3342 7624 3342 Size: 4282 3348 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3551 5437 5819 6454 Size: 53 83 186 936 2018 1886 382 635 Cuts at: 6454 275 Size: 1451 Fragments arranged by size: 2018 1886 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3364 6063 6461 6735 6882 7293 3364 Size: 2699 398 274 147 411 3701 Fragments arranged by size: 3701 2699 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7405 Fragments arranged by size: 7405 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2101 2961 3277 4079 4491 1186 Size: 915 860 316 802 412 4325 Fragments arranged by size: 4325 915 860 802 412 316 BseYI C'CCAG_C Cuts at: 4179 6833 7329 4179 Size: 2654 496 4480 Fragments arranged by size: 4480 2654 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3644 4170 1163 Size: 573 1908 526 4623 Fragments arranged by size: 4623 1908 573 526 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5080 5841 6869 1540 Size: 275 186 3079 761 1028 2301 Fragments arranged by size: 3079 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3996 4269 4684 5691 5776 6847 3996 Size: 273 415 1007 85 1071 4779 Fragments arranged by size: 4779 1071 1007 415 273 85 BsmI GAATG_Cn' Cuts at: 2829 5141 5234 2829 Size: 2312 93 5225 Fragments arranged by size: 5225 2312 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3729 3729 Size: 7630 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3996 4269 4684 4949 5691 5776 6847 Size: 399 2206 273 415 265 742 85 1071 Cuts at: 6847 1391 Size: 2174 Fragments arranged by size: 2206 2174 1071 742 415 399 273 265 85 BspEI T'CCGG_A Cuts at: 3364 7293 3364 Size: 3929 3701 BspHI T'CATG_A Cuts at: 4353 4765 5519 7249 4353 Size: 412 754 1730 4734 Fragments arranged by size: 4734 1730 754 412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2836 4985 844 Size: 1992 2149 3489 Fragments arranged by size: 3489 2149 1992 BsrBI CCG'CTC Cuts at: 1394 1823 5517 1394 Size: 429 3694 3507 Fragments arranged by size: 3694 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2925 6125 6299 65 Size: 1183 1677 3200 174 1396 Fragments arranged by size: 3200 1677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3660 6274 6461 1700 Size: 110 1850 2614 187 2869 Fragments arranged by size: 2869 2614 1850 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7630 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7630 BssSI C'ACGA_G Cuts at: 5690 6702 5690 Size: 1012 6618 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7630 BstBI TT'CG_AA Cuts at: 2007 6434 2007 Size: 4427 3203 BstEII G'GTnAC_C Cuts at: 1184 2636 1184 Size: 1452 6178 BstXI CCAn_nnnn'nTGG Cuts at: 2081 2081 Size: 7630 BstYI r'GATC_y Cuts at: 1972 2572 2761 3799 4290 4342 4540 4960 5327 Size: 600 189 1038 491 52 198 420 367 Cuts at: 5327 5712 5729 7181 7267 7279 7625 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1038 600 491 420 385 367 346 198 189 86 52 17 12 BstZ17I GTA'TAC Cuts at: 2542 2542 Size: 7630 BtgI C'CryG_G Cuts at: 513 1065 2022 3477 3689 4790 513 Size: 552 957 1455 212 1101 3353 Fragments arranged by size: 3353 1455 1101 957 552 212 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6891 Fragments arranged by size: 6891 683 56 BtsI GCAGTG_nn' Cuts at: 5146 5940 5960 5146 Size: 794 20 6816 Fragments arranged by size: 6816 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7630 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7595 DraI TTT'AAA Cuts at: 4934 4934 Size: 7630 DraIII CAC_nnn'GTG Cuts at: 2452 2452 Size: 7630 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6637 809 Size: 507 5321 1802 Fragments arranged by size: 5321 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3236 5077 5966 Size: 54 1425 50 275 18 1406 1841 889 Cuts at: 5966 7595 8 Size: 1629 43 Fragments arranged by size: 1841 1629 1425 1406 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5077 1537 Size: 275 3265 4090 Fragments arranged by size: 4090 3265 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2332 2718 3894 4365 4644 4812 5560 1353 Size: 979 386 1176 471 279 168 748 3423 Fragments arranged by size: 3423 1176 979 748 471 386 279 168 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6217 6591 6737 387 Size: 1064 138 4628 374 146 1280 Fragments arranged by size: 4628 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3994 4267 4682 3994 Size: 273 415 6942 Fragments arranged by size: 6942 415 273 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2228 2506 2600 2672 2822 3773 Size: 393 458 138 278 94 72 150 951 Cuts at: 3773 4023 4367 4844 5693 6290 7077 7345 1239 Size: 250 344 477 849 597 787 268 1524 Fragments arranged by size: 1524 951 849 787 597 477 458 393 344 278 268 250 150 138 94 72 EcoO109I rG'GnC_Cy Cuts at: 2070 3267 4569 4633 2070 Size: 1197 1302 64 5067 Fragments arranged by size: 5067 1302 1197 64 EcoRI G'AATT_C Cuts at: 4904 4939 5317 4904 Size: 35 378 7217 Fragments arranged by size: 7217 378 35 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2327 2561 3327 4111 2327 Size: 234 766 784 5846 Fragments arranged by size: 5846 784 766 234 FspI TGC'GCA Cuts at: 6136 6136 Size: 7630 HaeII r_GCGC'y Cuts at: 1678 3554 6777 1678 Size: 1876 3223 2531 Fragments arranged by size: 3223 2531 1876 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3192 3224 4142 4174 4199 Size: 32 135 32 1620 32 918 32 25 Cuts at: 4199 4231 4516 4548 4601 4633 4747 4779 6271 Size: 32 285 32 53 32 114 32 1492 Cuts at: 6271 6303 6345 6377 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1620 1492 918 285 135 114 53 42 32 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 3308 4974 5220 669 Size: 988 281 1370 1666 246 3079 Fragments arranged by size: 3079 1666 1370 988 281 246 HindIII A'AGCT_T Cuts at: 748 2754 3454 4807 748 Size: 2006 700 1353 3571 Fragments arranged by size: 3571 2006 1353 700 HpaI GTT'AAC Cuts at: 5220 5220 Size: 7630 KasI G'GCGC_C Cuts at: 3550 3550 Size: 7630 KpnI G_GTAC'C Cuts at: 4955 4955 Size: 7630 MfeI C'AATT_G Cuts at: 4025 5229 4025 Size: 1204 6426 MluI A'CGCG_T Cuts at: 7506 7506 Size: 7630 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4045 4242 6744 6928 1305 Size: 2740 197 2502 184 2007 Fragments arranged by size: 2740 2502 2007 197 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3238 10 Size: 54 1425 343 1406 4402 Fragments arranged by size: 4402 1425 1406 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5588 5947 6106 518 Size: 648 4422 359 159 2042 Fragments arranged by size: 4422 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2122 4202 5046 5723 6871 7116 1606 Size: 516 2080 844 677 1148 245 2120 Fragments arranged by size: 2120 2080 1148 844 677 516 245 NarI GG'CG_CC Cuts at: 3551 3551 Size: 7630 NcoI C'CATG_G Cuts at: 513 1065 2022 3689 513 Size: 552 957 1667 4454 Fragments arranged by size: 4454 1667 957 552 NdeI CA'TA_TG Cuts at: 387 4423 387 Size: 4036 3594 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7630 NotI GC'GGCC_GC Cuts at: 5077 5077 Size: 7630 NspI r_CATG'y Cuts at: 1132 1229 2189 3931 4988 6533 1132 Size: 97 960 1742 1057 1545 2229 Fragments arranged by size: 2229 1742 1545 1057 960 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7630 PciI A'CATG_T Cuts at: 6529 6529 Size: 7630 PflMI CCAn_nnn'nTGG Cuts at: 1108 2452 1108 Size: 1344 6286 PfoI T'CCnGG_A Cuts at: 2608 2859 3141 2608 Size: 251 282 7097 Fragments arranged by size: 7097 282 251 PmeI GTTT'AAAC Cuts at: 4934 4934 Size: 7630 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2370 2402 5699 5731 7212 7244 2370 Size: 32 3297 32 1481 32 2756 Fragments arranged by size: 3297 2756 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2070 2070 Size: 7630 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7630 PsiI TTA'TAA Cuts at: 5200 5200 Size: 7630 PstI C_TGCA'G Cuts at: 830 2127 4982 830 Size: 1297 2855 3478 Fragments arranged by size: 3478 2855 1297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7630 PvuII CAG'CTG Cuts at: 2122 4202 2122 Size: 2080 5550 SacI G_AGCT'C Cuts at: 3996 4269 4684 3996 Size: 273 415 6942 Fragments arranged by size: 6942 415 273 SalI G'TCGA_C Cuts at: 1936 3306 4972 1936 Size: 1370 1666 4594 Fragments arranged by size: 4594 1666 1370 SapI GCTCTTCn'nnn_ Cuts at: 4812 4812 Size: 7630 SbfI CC_TGCA'GG Cuts at: 4982 4982 Size: 7630 ScaI AGT'ACT Cuts at: 2103 5878 7590 2103 Size: 3775 1712 2143 Fragments arranged by size: 3775 2143 1712 SfcI C'TryA_G Cuts at: 826 1045 2123 3315 4125 4729 4773 4978 6113 Size: 219 1078 1192 810 604 44 205 1135 Cuts at: 6113 6794 6985 826 Size: 681 191 1471 Fragments arranged by size: 1471 1192 1135 1078 810 681 604 219 205 191 44 SfoI GGC'GCC Cuts at: 3552 3552 Size: 7630 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7630 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7630 SmaI CCC'GGG Cuts at: 1872 4957 1872 Size: 3085 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3804 5734 6635 6897 820 Size: 197 926 1861 1930 901 262 1553 Fragments arranged by size: 1930 1861 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4927 5421 493 Size: 4434 494 2702 Fragments arranged by size: 4434 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7630 SphI G_CATG'C Cuts at: 1132 3931 4988 1132 Size: 2799 1057 3774 Fragments arranged by size: 3774 2799 1057 SspI AAT'ATT Cuts at: 5 52 4138 5554 5 Size: 47 4086 1416 2081 Fragments arranged by size: 4086 2081 1416 47 StuI AGG'CCT Cuts at: 1517 3224 1517 Size: 1707 5923 StyI C'CwwG_G Cuts at: 513 1065 2022 2066 3225 3689 3912 5069 513 Size: 552 957 44 1159 464 223 1157 3074 Fragments arranged by size: 3074 1159 1157 957 552 464 223 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 2615 3006 4058 4829 5657 5674 Size: 1255 1031 4 391 1052 771 828 17 Cuts at: 5674 5827 6012 6457 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1052 1031 828 771 445 391 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2101 3242 5876 Size: 274 80 33 51 1443 124 1141 2634 Cuts at: 5876 7588 96 Size: 1712 138 Fragments arranged by size: 2634 1712 1443 1141 274 138 124 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2489 2521 2590 2622 7590 7622 2489 Size: 32 69 32 4968 32 2497 Fragments arranged by size: 4968 2497 69 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7630 XbaI T'CTAG_A Cuts at: 3439 3968 4966 3439 Size: 529 998 6103 Fragments arranged by size: 6103 998 529 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7630 XmaI C'CCGG_G Cuts at: 1870 4955 1870 Size: 3085 4545 XmnI GAAnn'nnTTC Cuts at: 5759 5759 Size: 7630 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5438 276 Size: 53 83 186 936 3904 2468 Fragments arranged by size: 3904 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbvCI BfrBI BsiWI Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI