(Circular) (Six-base) MAPSORT of: pFN21ASDA0752.seq Check: 996 from: 1 to: 5659 pFN21ASDA0752 5659 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2617 2719 2827 2617 Size: 102 108 5449 Fragments arranged by size: 5449 108 102 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2313 2459 3722 5106 5374 1632 Size: 681 146 1263 1384 268 1917 Fragments arranged by size: 1917 1384 1263 681 268 146 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5659 AlwNI CAG_nnn'CTG Cuts at: 2225 2399 2482 2597 2699 3402 4974 2225 Size: 174 83 115 102 703 1572 2910 Fragments arranged by size: 2910 1572 703 174 115 102 83 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2968 3194 3346 5455 1493 Size: 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2195 2984 1870 Size: 73 252 789 4545 Fragments arranged by size: 4545 789 252 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2339 2980 4435 618 Size: 325 217 627 552 641 1455 1842 Fragments arranged by size: 1842 1455 641 627 552 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2978 1391 Size: 697 890 4072 Fragments arranged by size: 4072 890 697 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2135 2370 2531 2568 2670 2769 928 Size: 971 236 235 161 37 102 99 3818 Fragments arranged by size: 3818 971 236 235 161 102 99 37 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3850 3884 1903 Size: 34 1913 34 3678 Fragments arranged by size: 3678 1913 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 2460 1548 Size: 912 4747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2645 2747 4270 Size: 107 122 71 631 605 973 102 1523 Cuts at: 4270 136 Size: 1525 Fragments arranged by size: 1525 1523 973 631 605 122 107 102 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 3720 4876 1790 Size: 1930 1156 2573 Fragments arranged by size: 2573 1930 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2221 2221 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2390 2588 2690 4319 1239 Size: 1151 198 102 1629 2579 Fragments arranged by size: 2579 1629 1151 198 102 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3784 4649 4947 3784 Size: 865 298 4496 Fragments arranged by size: 4496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2956 3450 493 Size: 711 1752 494 2702 Fragments arranged by size: 2702 1752 711 494 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 3848 4483 275 Size: 53 83 186 936 1933 382 635 1451 Fragments arranged by size: 1933 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2070 2100 2153 2183 1178 Size: 30 165 30 667 30 53 30 4654 Fragments arranged by size: 4654 667 165 53 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 2303 2387 2516 2519 2531 2585 2687 Size: 1096 21 84 129 3 12 54 102 Cuts at: 2687 1186 Size: 4158 Fragments arranged by size: 4158 1096 129 102 84 54 21 12 3 BseYI C'CCAG_C Cuts at: 2635 2737 2897 4862 5358 2635 Size: 102 160 1965 496 2936 Fragments arranged by size: 2936 1965 496 160 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2799 1163 Size: 573 1063 4023 Fragments arranged by size: 4023 1063 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 3720 3805 4876 2088 Size: 1632 85 1071 2871 Fragments arranged by size: 2871 1632 1071 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 BsmBI CGTCTCn'nnnn_ Cuts at: 2204 2204 Size: 5659 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2978 3720 3805 4876 1391 Size: 399 298 890 742 85 1071 2174 Fragments arranged by size: 2174 1071 890 742 399 298 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2617 2719 2827 3014 844 Size: 1773 102 108 187 3489 Fragments arranged by size: 3489 1773 187 108 102 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4154 4328 65 Size: 1183 2906 174 1396 Fragments arranged by size: 2906 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2910 4303 4490 1700 Size: 110 1100 1393 187 2869 Fragments arranged by size: 2869 1393 1100 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 2007 4463 2007 Size: 2456 3203 BstEII G'GTnAC_C Cuts at: 1184 2354 2552 1184 Size: 1170 198 4291 Fragments arranged by size: 4291 1170 198 BstYI r'GATC_y Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654 Size: 1017 367 385 17 1452 86 12 346 Cuts at: 5654 1972 Size: 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4150 Fragments arranged by size: 4150 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2138 487 Size: 683 56 912 4008 Fragments arranged by size: 4008 912 683 56 BtsI GCAGTG_nn' Cuts at: 3175 3969 3989 3175 Size: 794 20 4845 Fragments arranged by size: 4845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DraIII CAC_nnn'GTG Cuts at: 2153 2549 2153 Size: 396 5263 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2448 3106 3995 Size: 54 1425 50 275 18 618 658 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 658 618 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2847 3589 1353 Size: 1494 742 3423 Fragments arranged by size: 3423 1494 742 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2086 2086 Size: 5659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2313 2390 2459 2588 2690 3722 4319 Size: 393 681 77 69 129 102 1032 597 Cuts at: 4319 5106 5374 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 787 681 597 393 268 129 102 77 69 EcoO109I rG'GnC_Cy Cuts at: 2434 2509 2434 Size: 75 5584 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2548 2152 Size: 396 5263 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 4806 1678 Size: 3128 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2185 4300 4332 4374 Size: 32 135 32 581 32 2115 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 2115 581 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 2756 3258 2756 Size: 502 5157 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2054 3617 3976 4135 518 Size: 648 888 1563 359 159 2042 Fragments arranged by size: 2042 1563 888 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3075 3752 4900 5145 1606 Size: 1469 677 1148 245 2120 Fragments arranged by size: 2120 1469 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4150 Fragments arranged by size: 4150 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2579 2681 3017 4562 1132 Size: 97 1350 102 336 1545 2229 Fragments arranged by size: 2229 1545 1350 336 102 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 2549 1108 Size: 1441 4218 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3728 3760 5241 5273 3728 Size: 32 1481 32 4114 Fragments arranged by size: 4114 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2434 2509 2434 Size: 75 5584 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PstI C_TGCA'G Cuts at: 830 2824 3011 830 Size: 1994 187 3478 Fragments arranged by size: 3478 1994 187 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 SacI G_AGCT'C Cuts at: 2088 2088 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SanDI GG'GwC_CC Cuts at: 2434 2434 Size: 5659 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 2820 3007 4142 4823 5014 826 Size: 219 1775 187 1135 681 191 1471 Fragments arranged by size: 1775 1471 1135 681 219 191 187 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3763 4664 4926 820 Size: 197 926 1820 901 262 1553 Fragments arranged by size: 1820 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2956 3450 493 Size: 2463 494 2702 Fragments arranged by size: 2702 2463 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 3017 1132 Size: 1885 3774 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5659 StyI C'CwwG_G Cuts at: 513 1065 2022 2136 2172 2532 3098 513 Size: 552 957 114 36 360 566 3074 Fragments arranged by size: 3074 957 566 552 360 114 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2357 3686 3703 3856 4041 4486 325 Size: 1255 777 1329 17 153 185 445 1498 Fragments arranged by size: 1498 1329 1255 777 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2470 3905 5617 Size: 274 80 33 51 1443 493 1435 1712 Cuts at: 5617 96 Size: 138 Fragments arranged by size: 1712 1443 1435 493 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5619 5651 5619 Size: 32 5627 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2263 2995 2263 Size: 732 4927 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2356 2356 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 3788 3788 Size: 5659 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI