(Circular) (Six-base) MAPSORT of: pFN21ASDA0736.seq Check: 6162 from: 1 to: 6955 pFN21ASDA0736 6955 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4228 4228 Size: 6955 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4765 278 Size: 53 83 186 936 3229 2468 Fragments arranged by size: 3229 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2655 3813 4298 1937 Size: 718 1158 485 4594 Fragments arranged by size: 4594 1158 718 485 Acc65I G'GTAC_C Cuts at: 3270 4276 3270 Size: 1006 5949 AclI AA'CG_TT Cuts at: 1563 5082 5455 1563 Size: 3519 373 3063 Fragments arranged by size: 3519 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3164 4235 5018 6402 6670 1632 Size: 1532 1071 783 1384 268 1917 Fragments arranged by size: 1917 1532 1384 1071 783 268 AfeI AGC'GCT Cuts at: 2599 3278 2599 Size: 679 6276 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6758 AflIII A'CryG_T Cuts at: 1203 2610 3651 4032 5854 6831 1203 Size: 1407 1041 381 1822 977 1327 Fragments arranged by size: 1822 1407 1327 1041 977 381 AgeI A'CCGG_T Cuts at: 5786 5786 Size: 6955 AhdI GACnn_n'nnGTC Cuts at: 5684 5684 Size: 6955 AleI CACnn'nnGTG Cuts at: 1166 3476 1166 Size: 2310 4645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6955 AlwNI CAG_nnn'CTG Cuts at: 2339 2725 4243 4698 6270 2339 Size: 386 1518 455 1572 3024 Fragments arranged by size: 3024 1572 1518 455 386 ApaLI G'TGCA_C Cuts at: 5012 6168 5012 Size: 1156 5799 ApoI r'AATT_y Cuts at: 1493 3322 4264 4490 4642 6751 1493 Size: 1829 942 226 152 2109 1697 Fragments arranged by size: 2109 1829 1697 942 226 152 AseI AT'TA_AT Cuts at: 160 1032 5509 160 Size: 872 4477 1606 Fragments arranged by size: 4477 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6955 AvaI C'yCGr_G Cuts at: 1870 1943 2328 3485 4230 4280 1870 Size: 73 385 1157 745 50 4545 Fragments arranged by size: 4545 1157 745 385 73 50 AvrII C'CTAG_G Cuts at: 3301 3301 Size: 6955 BamHI G'GATC_C Cuts at: 4285 4652 4285 Size: 367 6588 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2371 3270 3800 4276 5731 Size: 325 217 627 584 899 530 476 1455 Cuts at: 5731 618 Size: 1842 Fragments arranged by size: 1842 1455 899 627 584 530 476 325 217 BanII G_rGCy'C Cuts at: 1391 3073 3095 4274 1391 Size: 1682 22 1179 4072 Fragments arranged by size: 4072 1682 1179 22 BbeI G_GCGC'C Cuts at: 2375 2375 Size: 6955 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2746 2842 3019 928 Size: 971 847 96 177 4864 Fragments arranged by size: 4864 971 847 177 96 BbvCI CC'TCA_GC Cuts at: 4180 4180 Size: 6955 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5146 5180 1903 Size: 34 3209 34 3678 Fragments arranged by size: 3678 3209 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4847 6057 4847 Size: 1210 5745 BclI T'GATC_A Cuts at: 1548 1548 Size: 6955 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3068 3996 5566 Size: 107 122 71 631 605 1396 928 1570 Cuts at: 5566 136 Size: 1525 Fragments arranged by size: 1570 1525 1396 928 631 605 122 107 71 BglII A'GATC_T Cuts at: 2298 6950 2298 Size: 4652 2303 BlpI GC'TnA_GC Cuts at: 4372 4372 Size: 6955 Bme1580I G_kGCm'C Cuts at: 1790 5016 6172 1790 Size: 3226 1156 2573 Fragments arranged by size: 3226 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3781 4033 1318 Size: 2463 252 4240 Fragments arranged by size: 4240 2463 252 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2199 4686 5644 Size: 416 53 83 399 24 782 2487 958 Cuts at: 5644 442 Size: 1753 Fragments arranged by size: 2487 1753 958 782 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6955 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2699 2699 Size: 6955 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2403 3040 3431 3898 5615 1239 Size: 1164 637 391 467 1717 2579 Fragments arranged by size: 2579 1717 1164 637 467 391 Bpu10I CC'TnA_GC Cuts at: 2079 3186 4180 2079 Size: 1107 994 4854 Fragments arranged by size: 4854 1107 994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2419 3316 3569 5080 5945 6243 2419 Size: 897 253 1511 865 298 3131 Fragments arranged by size: 3131 1511 897 865 298 253 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2034 4220 5618 882 Size: 617 535 2186 1398 2219 Fragments arranged by size: 2219 2186 1398 617 535 BsaAI yAC'GTr Cuts at: 493 1204 4252 4746 493 Size: 711 3048 494 2702 Fragments arranged by size: 3048 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3245 4127 6949 3245 Size: 882 2822 3251 Fragments arranged by size: 3251 2822 882 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2372 4762 5144 5779 Size: 53 83 186 936 839 2390 382 635 Cuts at: 5779 275 Size: 1451 Fragments arranged by size: 2390 1451 936 839 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5388 5786 6060 6207 6618 5388 Size: 398 274 147 411 5725 Fragments arranged by size: 5725 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2707 2737 1178 Size: 30 165 30 1304 30 5396 Fragments arranged by size: 5396 1304 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2186 2189 2238 2258 1186 Size: 1000 3 49 20 5883 Fragments arranged by size: 5883 1000 49 20 3 BseYI C'CCAG_C Cuts at: 2594 2707 3279 4117 6158 6654 2594 Size: 113 572 838 2041 496 2895 Fragments arranged by size: 2895 2041 838 572 496 113 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3652 3737 4146 1163 Size: 573 1916 85 409 3972 Fragments arranged by size: 3972 1916 573 409 85 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2509 4405 5166 6194 1540 Size: 275 186 508 1896 761 1028 2301 Fragments arranged by size: 2301 1896 1028 761 508 275 186 BsiHKAI G_wGCw'C Cuts at: 5016 5101 6172 5016 Size: 85 1071 5799 Fragments arranged by size: 5799 1071 85 BsmI GAATG_Cn' Cuts at: 2322 3906 4027 4096 4466 4559 2322 Size: 1584 121 69 370 93 4718 Fragments arranged by size: 4718 1584 370 121 93 69 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3073 3095 4274 5016 5101 6172 1391 Size: 399 1283 22 1179 742 85 1071 2174 Fragments arranged by size: 2174 1283 1179 1071 742 399 85 22 BspEI T'CCGG_A Cuts at: 6618 6618 Size: 6955 BspHI T'CATG_A Cuts at: 4844 6574 4844 Size: 1730 5225 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3139 3301 4228 4310 844 Size: 2295 162 927 82 3489 Fragments arranged by size: 3489 2295 927 162 82 BsrBI CCG'CTC Cuts at: 1394 1823 2356 4842 1394 Size: 429 533 2486 3507 Fragments arranged by size: 3507 2486 533 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2073 4008 5450 5624 65 Size: 1183 825 1935 1442 174 1396 Fragments arranged by size: 1935 1442 1396 1183 825 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2703 5599 5786 1700 Size: 110 893 2896 187 2869 Fragments arranged by size: 2896 2869 893 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6955 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6955 BssSI C'ACGA_G Cuts at: 5015 6027 5015 Size: 1012 5943 BstAPI GCAn_nnn'nTGC Cuts at: 794 2725 4243 794 Size: 1931 1518 3506 Fragments arranged by size: 3506 1931 1518 BstBI TT'CG_AA Cuts at: 2007 5759 2007 Size: 3752 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6955 BstXI CCAn_nnnn'nTGG Cuts at: 2110 2110 Size: 6955 BstYI r'GATC_y Cuts at: 1972 2298 3246 3534 4285 4652 5037 5054 6506 Size: 326 948 288 751 367 385 17 1452 Cuts at: 6506 6592 6604 6950 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 948 751 385 367 346 326 288 86 17 12 BstZ17I GTA'TAC Cuts at: 3814 3814 Size: 6955 BtgI C'CryG_G Cuts at: 513 1065 2022 2059 2503 513 Size: 552 957 37 444 4965 Fragments arranged by size: 4965 957 552 444 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6216 Fragments arranged by size: 6216 683 56 BtsI GCAGTG_nn' Cuts at: 2726 3459 3849 4471 5265 5285 2726 Size: 733 390 622 794 20 4396 Fragments arranged by size: 4396 794 733 622 390 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6955 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4060 4095 529 Size: 35 3496 35 3389 Fragments arranged by size: 3496 3389 35 35 DraI TTT'AAA Cuts at: 4259 4259 Size: 6955 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5962 809 Size: 507 4646 1802 Fragments arranged by size: 4646 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2500 2506 Size: 54 1425 50 275 18 270 400 6 Cuts at: 2506 3969 4402 5291 6920 8 Size: 1463 433 889 1629 43 Fragments arranged by size: 1629 1463 1425 889 433 400 275 270 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2506 4402 1537 Size: 275 694 1896 4090 Fragments arranged by size: 4090 1896 694 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2022 2804 3612 4885 1353 Size: 669 782 808 1273 3423 Fragments arranged by size: 3423 1273 808 782 669 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3428 5542 5916 6062 387 Size: 1064 138 1839 2114 374 146 1280 Fragments arranged by size: 2114 1839 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 3040 3164 3431 3898 4235 5018 Size: 393 771 637 124 267 467 337 783 Cuts at: 5018 5615 6402 6670 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 783 771 637 597 467 393 337 268 267 124 EcoO109I rG'GnC_Cy Cuts at: 2119 2232 2414 3264 2119 Size: 113 182 850 5810 Fragments arranged by size: 5810 850 182 113 EcoRI G'AATT_C Cuts at: 4264 4642 4264 Size: 378 6577 FspI TGC'GCA Cuts at: 5461 5461 Size: 6955 HaeII r_GCGC'y Cuts at: 1678 2375 2549 2601 3280 6102 1678 Size: 697 174 52 679 2822 2531 Fragments arranged by size: 2822 2531 697 679 174 52 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2707 2739 2823 2855 3208 Size: 32 135 32 1135 32 84 32 353 Cuts at: 3208 3236 3240 3268 3427 3449 3459 3481 3626 Size: 28 4 28 159 22 10 22 145 Cuts at: 3626 3658 5596 5628 5670 5702 1373 Size: 32 1938 32 42 32 2626 Fragments arranged by size: 2626 1938 1135 353 159 145 135 84 42 32 32 32 32 32 32 32 28 28 22 22 10 4 HincII GTy'rAC Cuts at: 669 1657 1938 2746 4038 4299 4545 669 Size: 988 281 808 1292 261 246 3079 Fragments arranged by size: 3079 1292 988 808 281 261 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6955 HpaI GTT'AAC Cuts at: 4545 4545 Size: 6955 KasI G'GCGC_C Cuts at: 2371 2371 Size: 6955 KpnI G_GTAC'C Cuts at: 3274 4280 3274 Size: 1006 5949 MfeI C'AATT_G Cuts at: 4554 4554 Size: 6955 MluI A'CGCG_T Cuts at: 6831 6831 Size: 6955 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2646 2652 2693 3234 3504 6069 6253 1305 Size: 1341 6 41 541 270 2565 184 2007 Fragments arranged by size: 2565 2007 1341 541 270 184 41 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2502 10 Size: 54 1425 343 670 4463 Fragments arranged by size: 4463 1425 670 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2669 3365 3476 4913 5272 5431 518 Size: 648 1503 696 111 1437 359 159 2042 Fragments arranged by size: 2042 1503 1437 696 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2945 3018 4112 4184 4371 5048 6196 6441 Size: 1339 73 1094 72 187 677 1148 245 Cuts at: 6441 1606 Size: 2120 Fragments arranged by size: 2120 1339 1148 1094 677 245 187 73 72 NarI GG'CG_CC Cuts at: 2372 2372 Size: 6955 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5446 Fragments arranged by size: 5446 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6955 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6955 NotI GC'GGCC_GC Cuts at: 4402 4402 Size: 6955 NspI r_CATG'y Cuts at: 1132 1229 2099 2614 2641 2917 3169 3506 4313 Size: 97 870 515 27 276 252 337 807 Cuts at: 4313 5858 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 870 807 515 337 276 252 97 27 PasI CC'CwG_GG Cuts at: 1348 2420 1348 Size: 1072 5883 PciI A'CATG_T Cuts at: 2610 5854 2610 Size: 3244 3711 PflMI CCAn_nnn'nTGG Cuts at: 1108 3454 1108 Size: 2346 4609 PfoI T'CCnGG_A Cuts at: 2133 4016 2133 Size: 1883 5072 PmeI GTTT'AAAC Cuts at: 4259 4259 Size: 6955 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3179 3211 5024 5056 6537 6569 3179 Size: 32 1813 32 1481 32 3565 Fragments arranged by size: 3565 1813 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2232 2414 3264 2232 Size: 182 850 5923 Fragments arranged by size: 5923 850 182 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6955 PsiI TTA'TAA Cuts at: 4525 4525 Size: 6955 PstI C_TGCA'G Cuts at: 830 4248 4307 830 Size: 3418 59 3478 Fragments arranged by size: 3478 3418 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6955 PvuII CAG'CTG Cuts at: 2945 3018 4112 4184 2945 Size: 73 1094 72 5716 Fragments arranged by size: 5716 1094 73 72 SalI G'TCGA_C Cuts at: 1936 4297 1936 Size: 2361 4594 SanDI GG'GwC_CC Cuts at: 2232 2414 2232 Size: 182 6773 SbfI CC_TGCA'GG Cuts at: 4307 4307 Size: 6955 ScaI AGT'ACT Cuts at: 3554 5203 6915 3554 Size: 1649 1712 3594 Fragments arranged by size: 3594 1712 1649 SexAI A'CCwGG_T Cuts at: 3266 3266 Size: 6955 SfcI C'TryA_G Cuts at: 826 1045 4244 4303 5438 6119 6310 826 Size: 219 3199 59 1135 681 191 1471 Fragments arranged by size: 3199 1471 1135 681 219 191 59 SfoI GGC'GCC Cuts at: 2373 2373 Size: 6955 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6955 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6955 SmaI CCC'GGG Cuts at: 1872 2330 3487 4232 4282 1872 Size: 458 1157 745 50 4545 Fragments arranged by size: 4545 1157 745 458 50 SmlI C'TyrA_G Cuts at: 820 1017 1943 2398 3295 3584 5059 5960 6222 Size: 197 926 455 897 289 1475 901 262 Cuts at: 6222 820 Size: 1553 Fragments arranged by size: 1553 1475 926 901 897 455 289 262 197 SnaBI TAC'GTA Cuts at: 493 4252 4746 493 Size: 3759 494 2702 Fragments arranged by size: 3759 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6955 SphI G_CATG'C Cuts at: 1132 2099 2641 4313 1132 Size: 967 542 1672 3774 Fragments arranged by size: 3774 1672 967 542 SspI AAT'ATT Cuts at: 5 52 2144 4879 5 Size: 47 2092 2735 2081 Fragments arranged by size: 2735 2092 2081 47 StuI AGG'CCT Cuts at: 1517 2646 3131 1517 Size: 1129 485 5341 Fragments arranged by size: 5341 1129 485 StyI C'CwwG_G Cuts at: 513 1065 2022 3301 4155 4195 4394 513 Size: 552 957 1279 854 40 199 3074 Fragments arranged by size: 3074 1279 957 854 552 199 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2967 3365 4180 4982 4999 5152 5337 Size: 1255 1387 398 815 802 17 153 185 Cuts at: 5337 5782 325 Size: 445 1498 Fragments arranged by size: 1498 1387 1255 815 802 445 398 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3552 3711 3743 Size: 274 80 33 51 1443 1575 159 32 Cuts at: 3743 5201 6913 96 Size: 1458 1712 138 Fragments arranged by size: 1712 1575 1458 1443 274 159 138 80 51 33 32 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2914 2946 6915 6947 2914 Size: 32 3969 32 2922 Fragments arranged by size: 3969 2922 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2129 1316 Size: 813 6142 XbaI T'CTAG_A Cuts at: 4291 4291 Size: 6955 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6955 XmaI C'CCGG_G Cuts at: 1870 2328 3485 4230 4280 1870 Size: 458 1157 745 50 4545 Fragments arranged by size: 4545 1157 745 458 50 XmnI GAAnn'nnTTC Cuts at: 5084 5084 Size: 6955 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4763 276 Size: 53 83 186 936 3229 2468 Fragments arranged by size: 3229 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI XcmI