(Circular) (Six-base) MAPSORT of: pFN21ASDA0733.seq Check: 3115 from: 1 to: 6808 pFN21ASDA0733 6808 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4618 278 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2233 2871 4151 1937 Size: 296 638 1280 4594 Fragments arranged by size: 4594 1280 638 296 Acc65I G'GTAC_C Cuts at: 4129 4129 Size: 6808 AclI AA'CG_TT Cuts at: 1563 4935 5308 1563 Size: 3372 373 3063 Fragments arranged by size: 3372 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2115 3066 4871 6255 6523 1632 Size: 474 9 951 1805 1384 268 1917 Fragments arranged by size: 1917 1805 1384 951 474 268 9 AfeI AGC'GCT Cuts at: 3901 3901 Size: 6808 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6611 AflIII A'CryG_T Cuts at: 1203 3285 3559 5707 6684 1203 Size: 2082 274 2148 977 1327 Fragments arranged by size: 2148 2082 1327 977 274 AgeI A'CCGG_T Cuts at: 5639 5639 Size: 6808 AhdI GACnn_n'nnGTC Cuts at: 2796 5537 2796 Size: 2741 4067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6808 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6808 AlwNI CAG_nnn'CTG Cuts at: 3805 4551 6123 3805 Size: 746 1572 4490 Fragments arranged by size: 4490 1572 746 ApaLI G'TGCA_C Cuts at: 4865 6021 4865 Size: 1156 5652 ApoI r'AATT_y Cuts at: 1493 2078 2203 2223 2470 2653 3763 4117 4343 Size: 585 125 20 247 183 1110 354 226 Cuts at: 4343 4495 6604 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1110 585 354 247 226 183 152 125 20 AseI AT'TA_AT Cuts at: 160 1032 5362 160 Size: 872 4330 1606 Fragments arranged by size: 4330 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 AvaI C'yCGr_G Cuts at: 1870 1943 3375 3876 4133 1870 Size: 73 1432 501 257 4545 Fragments arranged by size: 4545 1432 501 257 73 BamHI G'GATC_C Cuts at: 4138 4505 4138 Size: 367 6441 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3747 4129 5584 618 Size: 325 217 627 1960 382 1455 1842 Fragments arranged by size: 1960 1842 1455 627 382 325 217 BanII G_rGCy'C Cuts at: 1391 4021 4127 1391 Size: 2630 106 4072 Fragments arranged by size: 4072 2630 106 BbeI G_GCGC'C Cuts at: 3751 3751 Size: 6808 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2931 928 Size: 971 1032 4805 Fragments arranged by size: 4805 1032 971 BbvCI CC'TCA_GC Cuts at: 2754 2754 Size: 6808 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4999 5033 1903 Size: 34 3062 34 3678 Fragments arranged by size: 3678 3062 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2116 4700 5910 2116 Size: 2584 1210 3014 Fragments arranged by size: 3014 2584 1210 BclI T'GATC_A Cuts at: 1548 3505 1548 Size: 1957 4851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5419 136 Size: 107 122 71 631 605 3747 1525 Fragments arranged by size: 3747 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6803 6803 Size: 6808 BlpI GC'TnA_GC Cuts at: 4225 4225 Size: 6808 Bme1580I G_kGCm'C Cuts at: 1790 4869 6025 1790 Size: 3079 1156 2573 Fragments arranged by size: 3079 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6808 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4539 5497 442 Size: 416 53 83 399 24 3122 958 1753 Fragments arranged by size: 3122 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6808 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3052 3262 3282 3812 5468 1239 Size: 1813 210 20 530 1656 2579 Fragments arranged by size: 2579 1813 1656 530 210 20 Bpu10I CC'TnA_GC Cuts at: 2754 2754 Size: 6808 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2400 2409 3695 3978 4933 5798 6096 2400 Size: 9 1286 283 955 865 298 3112 Fragments arranged by size: 3112 1286 955 865 298 283 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5471 882 Size: 617 3972 2219 Fragments arranged by size: 3972 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4105 4599 493 Size: 711 2901 494 2702 Fragments arranged by size: 2901 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3002 6802 3002 Size: 3800 3008 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4615 4997 5632 Size: 53 83 186 936 2215 867 382 635 Cuts at: 5632 275 Size: 1451 Fragments arranged by size: 2215 1451 936 867 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5241 5639 5913 6060 6471 5241 Size: 398 274 147 411 5578 Fragments arranged by size: 5578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3412 3442 1178 Size: 30 165 30 2009 30 4544 Fragments arranged by size: 4544 2009 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3363 1186 Size: 2177 4631 BseYI C'CCAG_C Cuts at: 2724 3896 6011 6507 2724 Size: 1172 2115 496 3025 Fragments arranged by size: 3025 2115 1172 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6235 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4258 5019 6047 1540 Size: 275 186 2257 761 1028 2301 Fragments arranged by size: 2301 2257 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4021 4869 4954 6025 4021 Size: 848 85 1071 4804 Fragments arranged by size: 4804 1071 848 85 BsmI GAATG_Cn' Cuts at: 2716 3202 4319 4412 2716 Size: 486 1117 93 5112 Fragments arranged by size: 5112 1117 486 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4021 4127 4869 4954 6025 1391 Size: 399 2231 106 742 85 1071 2174 Fragments arranged by size: 2231 2174 1071 742 399 106 85 BspEI T'CCGG_A Cuts at: 6471 6471 Size: 6808 BspHI T'CATG_A Cuts at: 4697 6427 4697 Size: 1730 5078 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 4163 844 Size: 1232 2087 3489 Fragments arranged by size: 3489 2087 1232 BsrBI CCG'CTC Cuts at: 1394 1823 4695 1394 Size: 429 2872 3507 Fragments arranged by size: 3507 2872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5303 5477 65 Size: 1183 4055 174 1396 Fragments arranged by size: 4055 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5452 5639 1700 Size: 110 3642 187 2869 Fragments arranged by size: 3642 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6808 BssSI C'ACGA_G Cuts at: 4868 5880 4868 Size: 1012 5796 BstAPI GCAn_nnn'nTGC Cuts at: 794 3272 794 Size: 2478 4330 BstBI TT'CG_AA Cuts at: 2007 5612 2007 Size: 3605 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6808 BstYI r'GATC_y Cuts at: 1972 3599 4138 4505 4890 4907 6359 6445 6457 Size: 1627 539 367 385 17 1452 86 12 Cuts at: 6457 6803 1972 Size: 346 1977 Fragments arranged by size: 1977 1627 1452 539 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3177 3177 Size: 6808 BtgI C'CryG_G Cuts at: 513 1065 2022 2290 513 Size: 552 957 268 5031 Fragments arranged by size: 5031 957 552 268 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6069 Fragments arranged by size: 6069 683 56 BtsI GCAGTG_nn' Cuts at: 4324 5118 5138 4324 Size: 794 20 5994 Fragments arranged by size: 5994 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6808 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6773 DraI TTT'AAA Cuts at: 4112 4112 Size: 6808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5815 809 Size: 507 4499 1802 Fragments arranged by size: 4499 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2365 4255 5144 Size: 54 1425 50 275 18 535 1890 889 Cuts at: 5144 6773 8 Size: 1629 43 Fragments arranged by size: 1890 1629 1425 889 535 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4255 1537 Size: 275 2443 4090 Fragments arranged by size: 4090 2443 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2290 3085 3248 4738 1353 Size: 937 795 163 1490 3423 Fragments arranged by size: 3423 1490 937 795 163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5395 5769 5915 387 Size: 1064 138 3806 374 146 1280 Fragments arranged by size: 3806 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4019 4019 Size: 6808 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2115 3052 3066 3262 3282 3812 Size: 393 474 9 937 14 196 20 530 Cuts at: 3812 4871 5468 6255 6523 1239 Size: 1059 597 787 268 1524 Fragments arranged by size: 1524 1059 937 787 597 530 474 393 268 196 20 14 9 EcoO109I rG'GnC_Cy Cuts at: 2981 3308 3938 2981 Size: 327 630 5851 Fragments arranged by size: 5851 630 327 EcoRI G'AATT_C Cuts at: 4117 4495 4117 Size: 378 6430 EcoRV GAT'ATC Cuts at: 2180 2180 Size: 6808 FspI TGC'GCA Cuts at: 5314 5314 Size: 6808 HaeII r_GCGC'y Cuts at: 1678 3751 3903 5955 1678 Size: 2073 152 2052 2531 Fragments arranged by size: 2531 2073 2052 152 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2782 2814 3146 Size: 32 135 32 683 32 495 32 332 Cuts at: 3146 3178 3292 3324 3369 3401 3953 3985 5449 Size: 32 114 32 45 32 552 32 1464 Cuts at: 5449 5481 5523 5555 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1464 683 552 495 332 135 114 45 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3173 4152 4398 669 Size: 988 281 1235 979 246 3079 Fragments arranged by size: 3079 1235 988 979 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6808 HpaI GTT'AAC Cuts at: 4398 4398 Size: 6808 KasI G'GCGC_C Cuts at: 3747 3747 Size: 6808 KpnI G_GTAC'C Cuts at: 4133 4133 Size: 6808 MfeI C'AATT_G Cuts at: 4407 4407 Size: 6808 MluI A'CGCG_T Cuts at: 6684 6684 Size: 6808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5922 6106 1305 Size: 4617 184 2007 Fragments arranged by size: 4617 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2367 10 Size: 54 1425 343 535 4451 Fragments arranged by size: 4451 1425 535 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2613 4766 5125 5284 518 Size: 648 1447 2153 359 159 2042 Fragments arranged by size: 2153 2042 1447 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3805 4224 4901 6049 6294 1606 Size: 2199 419 677 1148 245 2120 Fragments arranged by size: 2199 2120 1148 677 419 245 NarI GG'CG_CC Cuts at: 3748 3748 Size: 6808 NcoI C'CATG_G Cuts at: 513 1065 2022 2290 513 Size: 552 957 268 5031 Fragments arranged by size: 5031 957 552 268 NdeI CA'TA_TG Cuts at: 387 387 Size: 6808 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6808 NotI GC'GGCC_GC Cuts at: 4255 4255 Size: 6808 NspI r_CATG'y Cuts at: 1132 1229 2119 2878 3289 3563 4166 5711 1132 Size: 97 890 759 411 274 603 1545 2229 Fragments arranged by size: 2229 1545 890 759 603 411 274 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6808 PciI A'CATG_T Cuts at: 3285 3559 5707 3285 Size: 274 2148 4386 Fragments arranged by size: 4386 2148 274 PflMI CCAn_nnn'nTGG Cuts at: 1108 2486 1108 Size: 1378 5430 PfoI T'CCnGG_A Cuts at: 3278 3278 Size: 6808 PmeI GTTT'AAAC Cuts at: 4112 4112 Size: 6808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4877 4909 6390 6422 4877 Size: 32 1481 32 5263 Fragments arranged by size: 5263 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2981 3308 3938 2981 Size: 327 630 5851 Fragments arranged by size: 5851 630 327 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6808 PsiI TTA'TAA Cuts at: 4378 4378 Size: 6808 PspXI vC'TCGA_Gb Cuts at: 3375 3375 Size: 6808 PstI C_TGCA'G Cuts at: 830 3448 4160 830 Size: 2618 712 3478 Fragments arranged by size: 3478 2618 712 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6808 PvuII CAG'CTG Cuts at: 3805 3805 Size: 6808 SacI G_AGCT'C Cuts at: 4021 4021 Size: 6808 SalI G'TCGA_C Cuts at: 1936 4150 1936 Size: 2214 4594 SbfI CC_TGCA'GG Cuts at: 4160 4160 Size: 6808 ScaI AGT'ACT Cuts at: 5056 6768 5056 Size: 1712 5096 SfcI C'TryA_G Cuts at: 826 1045 3444 4156 5291 5972 6163 826 Size: 219 2399 712 1135 681 191 1471 Fragments arranged by size: 2399 1471 1135 712 681 219 191 SfoI GGC'GCC Cuts at: 3749 3749 Size: 6808 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6808 SmaI CCC'GGG Cuts at: 1872 4135 1872 Size: 2263 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2415 2424 3375 3674 3993 4912 Size: 197 926 472 9 951 299 319 919 Cuts at: 4912 5813 6075 820 Size: 901 262 1553 Fragments arranged by size: 1553 951 926 919 901 472 319 299 262 197 9 SnaBI TAC'GTA Cuts at: 493 4105 4599 493 Size: 3612 494 2702 Fragments arranged by size: 3612 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6808 SphI G_CATG'C Cuts at: 1132 4166 1132 Size: 3034 3774 SspI AAT'ATT Cuts at: 5 52 2971 3529 3925 4732 5 Size: 47 2919 558 396 807 2081 Fragments arranged by size: 2919 2081 807 558 396 47 StuI AGG'CCT Cuts at: 1517 2671 1517 Size: 1154 5654 StyI C'CwwG_G Cuts at: 513 1065 2022 2029 2290 4091 4247 513 Size: 552 957 7 261 1801 156 3074 Fragments arranged by size: 3074 1801 957 552 261 156 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4835 4852 5005 5190 5635 325 Size: 1255 3255 17 153 185 445 1498 Fragments arranged by size: 3255 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2171 2632 4040 Size: 274 80 33 51 1443 194 461 1408 Cuts at: 4040 5054 6766 96 Size: 1014 1712 138 Fragments arranged by size: 1712 1443 1408 1014 461 274 194 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6768 6800 6768 Size: 32 6776 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6808 XbaI T'CTAG_A Cuts at: 4144 4144 Size: 6808 XhoI C'TCGA_G Cuts at: 1943 3375 1943 Size: 1432 5376 XmaI C'CCGG_G Cuts at: 1870 4133 1870 Size: 2263 4545 XmnI GAAnn'nnTTC Cuts at: 2385 3031 3660 4937 2385 Size: 646 629 1277 4256 Fragments arranged by size: 4256 1277 646 629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4616 276 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI