Restriction map for
pFN21ASDA0724
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# NO CUT ENZYMES ( 78 enzymes) #
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AccIII AclI AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AviII BamHI BbeI BbrPI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BsrDI BssHII BssSI BstBI
BstEII BstPI ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HindIII HpaI KasI KspI MluI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AatII 1441 GACGTC GACGT^C
Acc65I 210 GGTACC G^GTACC
AccII 2794 CGCG CG^CG
AcsI 1262 GAATTT [GA]^AATT[CT]
AcyI 1441 GACGTC G[GA]^CG[CT]C
AflII 1819 CTTAAG C^TTAAG
AhdI 2243 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 2344 GGGCCC GGGCC^C
ApoI 1262 GAATTT [GA]^AATT[CT]
Asp700I 2832 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 210 GGTACC G^GTACC
AspEI 2243 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1519 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 959 CCCGGG C^[CT]CG[GA]G
AvrII 1611 CCTAGG C^CTAGG
BcgI 2784 GCAGATCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2769 GGATAC GTATCC(6/5)
BfrI 1819 CTTAAG C^TTAAG
BglI 382 GCCTCACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1611 CCTAGG C^CTAGG
BsaAI 823 TACGTG [CT]AC^GT[GA]
BsaBI 1491 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1441 GACGTC G[GA]^CG[CT]C
BsiEI 2583 CGGTCG CG[GA][CT]^CG
BsmI 792 GAATGC GAATGC(1/-1)
BsoBI 959 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2201 TGTACA T^GTACA
BsrBI 4 GAGCGG CCGCTC(-3/-3)
BsrFI 1157 ACCGGC [GA]^CCGG[CT]
BsrGI 2201 TGTACA T^GTACA
Bst1107I 1141 GTATAC GTA^TAC
BstUI 2794 CGCG CG^CG
BstZ17I 1141 GTATAC GTA^TAC
Cfr10I 1157 ACCGGC [GA]^CCGG[CT]
DrdI 1564 GACCACCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2243 GACAGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2388 CCTGGCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2659 GATATC GAT^ATC
Hin1I 1441 GACGTC G[GA]^CG[CT]C
KpnI 210 GGTACC GGTAC^C
MamI 1491 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2288 CAATTG C^AATTG
MunI 2288 CAATTG C^AATTG
MvnI 2794 CGCG CG^CG
NruI 2793 TCGCGA TCG^CGA
PflFI 1519 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2016 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1954 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 224 AGTACT AGT^ACT
SexAI 2730 ACCTGGT A^CC[AT]GGT
SmaI 959 CCCGGG CCC^GGG
SphI 1605 GCATGC GCATG^C
SspBI 2201 TGTACA T^GTACA
Tth111I 1519 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 959 CCCGGG C^CCGGG
XmaI 959 CCCGGG C^CCGGG
XmnI 2832 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccI 1141 GTATAC GT^[AC][GT]AC
1165 GTCTAC
AocI 520 CCTGAGG CC^T[ACGT]AGG
2485 CCTGAGG
BbvCI 2751 CCTCAGC CCTCAGC(-5/-2)
454 GCTGAGG
BclI 1577 TGATCA T^GATCA
1974 TGATCA
BfaI 490 CTAG C^TAG
1612 CTAG
BglII 254 AGATCT A^GATCT
1658 AGATCT
BlpI 1326 GCTCAGC GC^T[ACGT]AGC
2073 GCTGAGC
Bpu1102I 1326 GCTCAGC GC^T[ACGT]AGC
2073 GCTGAGC
BstAPI 189 GCAGCTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
882 GCAGGCAGTGC
Bsu36I 520 CCTGAGG CC^T[ACGT]AGG
2485 CCTGAGG
CelII 1326 GCTCAGC GC^T[ACGT]AGC
2073 GCTGAGC
Eco81I 520 CCTGAGG CC^T[ACGT]AGG
2485 CCTGAGG
FbaI 1577 TGATCA T^GATCA
1974 TGATCA
HgaI 2142 GACGC GACGC(5/10)
654 GCGTC
HincII 1009 GTCAAC GT[CT]^[GA]AC
1444 GTCAAC
HindII 1009 GTCAAC GT[CT]^[GA]AC
1444 GTCAAC
MaeI 490 CTAG C^TAG
1612 CTAG
MseI 1820 TTAA T^TAA
2138 TTAA
SacI 760 GAGCTC GAGCT^C
1541 GAGCTC
TaqI 767 TCGA T^CGA
2241 TCGA
TfiI 1229 GATTC G^A[AT]TC
2371 GATTC
Tru9I 1820 TTAA T^TAA
2138 TTAA
Tsp509I 1263 AATT ^AATT
2289 AATT
TthHB8I 767 TCGA T^CGA
2241 TCGA
XcmI 575 CCAGCCTGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2021 CCAACCCCGTGGTGG
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
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AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanI BanII
BbsI BbvI BcnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspMI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RcaI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TseI Tsp45I
TspRI Van91I XhoII