Restriction map for
pFN21ASDA0722
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AcsI AflIII
AgeI AhdI Alw44I ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BbrPI BciVI BclI
BglII BlnI BsaAI BsaBI BseAI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI Eam1105I
EcoRI EcoRV EcoT22I FbaI HincII
HindII HindIII HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SfuI SgrAI SnaBI SpeI
SphI SspI StuI SwaI TfiI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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Acc65I 509 GGTACC G^GTACC
AflII 2107 CTTAAG C^TTAAG
Aor51HI 2873 AGCGCT AGC^GCT
Asp718I 509 GGTACC G^GTACC
AspI 1066 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1469 TGGCCA TGG^CCA
BfaI 1936 CTAG C^TAG
BfrI 2107 CTTAAG C^TTAAG
BsaWI 1973 ACCGGA [AT]^CCGG[AT]
BsmI 2909 GCATTC GAATGC(1/-1)
BsrDI 2442 CATTGC GCAATG(2/0)
BstEII 1096 GGTGACC G^GT[ACGT]ACC
BstPI 1096 GGTGACC G^GT[ACGT]ACC
Eco47III 2873 AGCGCT AGC^GCT
EcoO65I 1096 GGTGACC G^GT[ACGT]ACC
FseI 1495 GGCCGGCC GGCCGG^CC
KpnI 509 GGTACC GGTAC^C
KspI 12 CCGCGG CCGC^GG
MaeI 1936 CTAG C^TAG
MluNI 1469 TGGCCA TGG^CCA
MscI 1469 TGGCCA TGG^CCA
NspI 3056 ACATGC [GA]CATG^[CT]
PflFI 1066 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 243 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 711 GACGTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 12 CCGCGG CCGC^GG
SbfI 2157 CCTGCAGG CCTGCA^GG
ScaI 278 AGTACT AGT^ACT
Sse8387I 2157 CCTGCAGG CCTGCA^GG
Tsp509I 256 AATT ^AATT
Tth111I 1066 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 243 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 262 GTCTAC GT^[AC][GT]AC
631 GTCTAC
AlwI 2526 GATCC GGATC(4/5)
2760 GATCC
AocI 1241 CCTCAGG CC^T[ACGT]AGG
2491 CCTGAGG
AviII 320 TGCGCA TGC^GCA
2807 TGCGCA
BbvCI 2186 CCTCAGC CCTCAGC(-5/-2)
1108 GCTGAGG
BcgI 2436 CGACCCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
797 GCATGGACTTCG
Bsp1407I 557 TGTACA T^GTACA
2825 TGTACA
BsrGI 557 TGTACA T^GTACA
2825 TGTACA
BstXI 554 CCATGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1403 CCAGCCCCCTGG
BstYI 2525 AGATCC [GA]^GATC[CT]
2759 AGATCC
Bsu36I 1241 CCTCAGG CC^T[ACGT]AGG
2491 CCTGAGG
DrdI 1063 GACGACTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1684 GACTTGCACGTC
Eco81I 1241 CCTCAGG CC^T[ACGT]AGG
2491 CCTGAGG
FspI 320 TGCGCA TGC^GCA
2807 TGCGCA
MflI 2525 AGATCC [GA]^GATC[CT]
2759 AGATCC
MseI 143 TTAA T^TAA
2108 TTAA
NcoI 2010 CCATGG C^CATGG
2171 CCATGG
SacI 1158 GAGCTC GAGCT^C
2401 GAGCTC
SapI 348 GCTCTTC GCTCTTC(1/4)
2348 GCTCTTC
SfiI 1246 GGCCGGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2059 GGCCCCTTTGGCC
SmlI 1517 CTCAAG C^T[CT][GA]AG
2107 CTTAAG
SspBI 557 TGTACA T^GTACA
2825 TGTACA
Tru9I 143 TTAA T^TAA
2108 TTAA
XhoII 2525 AGATCC [GA]^GATC[CT]
2759 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SexAI SfaNI SfcI SfoI SmaI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XmaCI XmaI