Restriction map for
pFN21ASDA0722
(Linear) (Six-base) MAPSORT of: KIAA0722.seq Check: 126 from: 1 to: 3150
KIAA0722.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1262 1299 2790 3150
Size: 1262 37 1491 360
Fragments arranged by size:
1491 1262 360 37
AccI GT'mk_AC
Cuts at: 0 263 632 3150
Size: 263 369 2518
Fragments arranged by size:
2518 369 263
Acc65I G'GTAC_C
Cuts at: 0 509 3150
Size: 509 2641
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 2608 2731 2925 3150
Size: 220 2388 123 194 225
Fragments arranged by size:
2388 225 220 194 123
AfeI AGC'GCT
Cuts at: 0 2875 3150
Size: 2875 275
AflII C'TTAA_G
Cuts at: 0 2107 3150
Size: 2107 1043
AleI CACnn'nnGTG
Cuts at: 0 1309 3150
Size: 1309 1841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2987 3150
Size: 2987 163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2971 3003 3150
Size: 2971 32 147
Fragments arranged by size:
2971 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 174 642 684 969 1923 2398 2856 3150
Size: 174 468 42 285 954 475 458 294
Fragments arranged by size:
954 475 468 458 294 285 174 42
ApaI G_GGCC'C
Cuts at: 0 2029 2062 2305 2332 2378 2620 3150
Size: 2029 33 243 27 46 242 530
Fragments arranged by size:
2029 530 243 242 46 33 27
AvaI C'yCGr_G
Cuts at: 0 568 731 891 1022 1621 2139 2277 3150
Size: 568 163 160 131 599 518 138 873
Fragments arranged by size:
873 599 568 518 163 160 138 131
BanI G'GyrC_C
Cuts at: 0 73 370 509 622 654 1113 1279 2233
Size: 73 297 139 113 32 459 166 954
Cuts at: 2233 2367 3150
Size: 134 783
Fragments arranged by size:
954 783 459 297 166 139 134 113
73 32
BanII G_rGCy'C
Cuts at: 0 8 1162 1813 1867 2029 2062 2223 2272
Size: 8 1154 651 54 162 33 161 49
Cuts at: 2272 2305 2332 2378 2405 2620 3150
Size: 33 27 46 27 215 530
Fragments arranged by size:
1154 651 530 215 162 161 54 49
46 33 33 27 27 8
BbeI G_GCGC'C
Cuts at: 0 77 374 658 1117 1283 3150
Size: 77 297 284 459 166 1867
Fragments arranged by size:
1867 459 297 284 166 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 81 982 1909 2250 3150
Size: 81 901 927 341 900
Fragments arranged by size:
927 901 900 341 81
BbvCI CC'TCA_GC
Cuts at: 0 1109 2187 3150
Size: 1109 1078 963
Fragments arranged by size:
1109 1078 963
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 786 820 2425 2459 3150
Size: 786 34 1605 34 691
Fragments arranged by size:
1605 786 691 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 17 991 1253 1412 2066 2336 2605 3150
Size: 17 974 262 159 654 270 269 545
Fragments arranged by size:
974 654 545 270 269 262 159 17
BlpI GC'TnA_GC
Cuts at: 0 328 1451 2668 3150
Size: 328 1123 1217 482
Fragments arranged by size:
1217 1123 482 328
Bme1580I G_kGCm'C
Cuts at: 0 878 1886 2029 2062 2236 2305 2332 2378
Size: 878 1008 143 33 174 69 27 46
Cuts at: 2378 2620 3150
Size: 242 530
Fragments arranged by size:
1008 878 530 242 174 143 69 46
33 27
BmgBI CAC'GTC
Cuts at: 0 1692 2568 3150
Size: 1692 876 582
Fragments arranged by size:
1692 876 582
BmrI ACTGGGnnnn_n'
Cuts at: 0 1112 1229 1291 1350 3150
Size: 1112 117 62 59 1800
Fragments arranged by size:
1800 1112 117 62 59
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 894 1248 1569 2396 2476 2593 3061
Size: 499 395 354 321 827 80 117 468
Cuts at: 3061 3150
Size: 89
Fragments arranged by size:
827 499 468 395 354 321 117 89
80
Bpu10I CC'TnA_GC
Cuts at: 0 1109 1535 2187 2399 2857 3150
Size: 1109 426 652 212 458 293
Fragments arranged by size:
1109 652 458 426 293 212
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1502 3150
Size: 1502 1648
BsaI GGTCTCn'nnnn_
Cuts at: 0 19 730 2491 3150
Size: 19 711 1761 659
Fragments arranged by size:
1761 711 659 19
BsaHI Gr'CG_yC
Cuts at: 0 74 371 655 1114 1280 1463 1558 1960
Size: 74 297 284 459 166 183 95 402
Cuts at: 1960 3150
Size: 1190
Fragments arranged by size:
1190 459 402 297 284 183 166 95
74
BsaWI w'CCGG_w
Cuts at: 0 1973 3150
Size: 1973 1177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 922 952 1191 1221 1497 1527 3150
Size: 922 30 239 30 276 30 1623
Fragments arranged by size:
1623 922 276 239 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1505 1602 2509 3150
Size: 1505 97 907 641
Fragments arranged by size:
1505 907 641 97
BseYI C'CCAG_C
Cuts at: 0 177 584 1647 1769 1909 2227 2391 3150
Size: 177 407 1063 122 140 318 164 759
Fragments arranged by size:
1063 759 407 318 177 164 140 122
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 370 997 1170 1333 1601 1645 2194
Size: 295 75 627 173 163 268 44 549
Cuts at: 2194 2581 2974 2983 3150
Size: 387 393 9 167
Fragments arranged by size:
627 549 393 387 295 268 173 167
163 75 44 9
BsiEI CG_ry'CG
Cuts at: 0 12 453 1197 3150
Size: 12 441 744 1953
Fragments arranged by size:
1953 744 441 12
BsiHKAI G_wGCw'C
Cuts at: 0 1162 1628 2290 2405 2519 3150
Size: 1162 466 662 115 114 631
Fragments arranged by size:
1162 662 631 466 115 114
BsmI GAATG_Cn'
Cuts at: 0 2909 3150
Size: 2909 241
Bsp1286I G_dGCh'C
Cuts at: 0 8 878 1162 1628 1813 1867 1886 2029
Size: 8 870 284 466 185 54 19 143
Cuts at: 2029 2062 2223 2236 2272 2290 2305 2332 2378
Size: 33 161 13 36 18 15 27 46
Cuts at: 2378 2405 2519 2620 3150
Size: 27 114 101 530
Fragments arranged by size:
870 530 466 284 185 161 143 114
101 54 46 36 33 27 27 19
18 15 13 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 692 1262 1299 1349 1379 1583 1811
Size: 317 375 570 37 50 30 204 228
Cuts at: 1811 1931 2108 2790 3150
Size: 120 177 682 360
Fragments arranged by size:
682 570 375 360 317 228 204 177
120 50 37 30
BsrBI CCG'CTC
Cuts at: 0 1539 1553 2282 3114 3150
Size: 1539 14 729 832 36
Fragments arranged by size:
1539 832 729 36 14
BsrDI GCAATG_nn'
Cuts at: 0 2441 3150
Size: 2441 709
BsrFI r'CCGG_y
Cuts at: 0 448 1496 2069 2767 3150
Size: 448 1048 573 698 383
Fragments arranged by size:
1048 698 573 448 383
BsrGI T'GTAC_A
Cuts at: 0 557 2825 3150
Size: 557 2268 325
Fragments arranged by size:
2268 557 325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 318 969 1274 2777 2805 2961 3057 3104
Size: 318 651 305 1503 28 156 96 47
Cuts at: 3104 3150
Size: 46
Fragments arranged by size:
1503 651 318 305 156 96 47 46
28
BstEII G'GTnAC_C
Cuts at: 0 1096 3150
Size: 1096 2054
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 1410 3150
Size: 561 849 1740
Fragments arranged by size:
1740 849 561
BstYI r'GATC_y
Cuts at: 0 2525 2759 3150
Size: 2525 234 391
Fragments arranged by size:
2525 391 234
Bsu36I CC'TnA_GG
Cuts at: 0 1242 2492 3150
Size: 1242 1250 658
Fragments arranged by size:
1250 1242 658
BtgI C'CryG_G
Cuts at: 0 12 29 69 1191 1721 1779 2010 2171
Size: 12 17 40 1122 530 58 231 161
Cuts at: 2171 2264 3150
Size: 93 886
Fragments arranged by size:
1122 886 530 231 161 93 58 40
17 12
BtsI GCAGTG_nn'
Cuts at: 0 1158 2921 3150
Size: 1158 1763 229
Fragments arranged by size:
1763 1158 229
DraIII CAC_nnn'GTG
Cuts at: 0 2367 2952 3095 3150
Size: 2367 585 143 55
Fragments arranged by size:
2367 585 143 55
DrdI GACnn_nn'nnGTC
Cuts at: 0 1069 1690 3150
Size: 1069 621 1460
Fragments arranged by size:
1460 1069 621
EaeI y'GGCC_r
Cuts at: 0 9 66 299 450 533 1194 1469 1540
Size: 9 57 233 151 83 661 275 71
Cuts at: 1540 2038 2067 2648 2717 3150
Size: 498 29 581 69 433
Fragments arranged by size:
661 581 498 433 275 233 151 83
71 69 57 29 9
EagI C'GGCC_G
Cuts at: 0 9 450 1194 3150
Size: 9 441 744 1956
Fragments arranged by size:
1956 744 441 9
EarI CTCTTCn'nnn_
Cuts at: 0 355 1053 2355 3150
Size: 355 698 1302 795
Fragments arranged by size:
1302 795 698 355
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 619 1687 3150
Size: 619 1068 1463
Fragments arranged by size:
1463 1068 619
EcoICRI GAG'CTC
Cuts at: 0 1160 2403 3150
Size: 1160 1243 747
Fragments arranged by size:
1243 1160 747
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 499 894 1248 1569 2396 2476 2593
Size: 220 279 395 354 321 827 80 117
Cuts at: 2593 2608 2731 2925 3061 3150
Size: 15 123 194 136 89
Fragments arranged by size:
827 395 354 321 279 220 194 136
123 117 89 80 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 849 1604 1993 2411 2491 3150
Size: 849 755 389 418 80 659
Fragments arranged by size:
849 755 659 418 389 80
EcoO109I rG'GnC_Cy
Cuts at: 0 57 294 681 1595 2002 2026 2049 2058
Size: 57 237 387 914 407 24 23 9
Cuts at: 2058 2059 2301 2302 2329 2375 2388 2478 2616
Size: 1 242 1 27 46 13 90 138
Cuts at: 2616 2617 2853 3027 3150
Size: 1 236 174 123
Fragments arranged by size:
914 407 387 242 237 236 174 138
123 90 57 46 27 24 23 13
9 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 675 2079 3150
Size: 675 1404 1071
Fragments arranged by size:
1404 1071 675
FseI GG_CCGG'CC
Cuts at: 0 1500 3150
Size: 1500 1650
FspI TGC'GCA
Cuts at: 0 322 2809 3150
Size: 322 2487 341
Fragments arranged by size:
2487 341 322
FspAI rTGC'GCAy
Cuts at: 0 322 3150
Size: 322 2828
HaeII r_GCGC'y
Cuts at: 0 77 374 658 1117 1283 1670 2600 2877
Size: 77 297 284 459 166 387 930 277
Cuts at: 2877 3130 3150
Size: 253 20
Fragments arranged by size:
930 459 387 297 284 277 253 166
77 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1191 1223 1950 1982 2713 2745 3150
Size: 1191 32 727 32 731 32 405
Fragments arranged by size:
1191 731 727 405 32 32 32
KasI G'GCGC_C
Cuts at: 0 73 370 654 1113 1279 3150
Size: 73 297 284 459 166 1871
Fragments arranged by size:
1871 459 297 284 166 73
KpnI G_GTAC'C
Cuts at: 0 513 3150
Size: 513 2637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1503 3150
Size: 464 1039 1647
Fragments arranged by size:
1647 1039 464
MscI TGG'CCA
Cuts at: 0 1471 3150
Size: 1471 1679
MslI CAynn'nnrTG
Cuts at: 0 559 1309 2314 3150
Size: 559 750 1005 836
Fragments arranged by size:
1005 836 750 559
MspA1I CmG'CkG
Cuts at: 0 14 750 759 969 1003 2191 2326 2631
Size: 14 736 9 210 34 1188 135 305
Cuts at: 2631 2697 2895 3150
Size: 66 198 255
Fragments arranged by size:
1188 736 305 255 210 198 135 66
34 14 9
NaeI GCC'GGC
Cuts at: 0 450 1498 2769 3150
Size: 450 1048 1271 381
Fragments arranged by size:
1271 1048 450 381
NarI GG'CG_CC
Cuts at: 0 74 371 655 1114 1280 3150
Size: 74 297 284 459 166 1870
Fragments arranged by size:
1870 459 297 284 166 74
NcoI C'CATG_G
Cuts at: 0 2010 2171 3150
Size: 2010 161 979
Fragments arranged by size:
2010 979 161
NgoMIV G'CCGG_C
Cuts at: 0 448 1496 2767 3150
Size: 448 1048 1271 383
Fragments arranged by size:
1271 1048 448 383
NspI r_CATG'y
Cuts at: 0 3060 3150
Size: 3060 90
PasI CC'CwG_GG
Cuts at: 0 951 1410 1731 2298 3150
Size: 951 459 321 567 852
Fragments arranged by size:
951 852 567 459 321
PflMI CCAn_nnn'nTGG
Cuts at: 0 249 3150
Size: 249 2901
PfoI T'CCnGG_A
Cuts at: 0 242 2412 2570 2882 2900 3038 3150
Size: 242 2170 158 312 18 138 112
Fragments arranged by size:
2170 312 242 158 138 112 18
PpuMI rG'GwC_Cy
Cuts at: 0 57 294 681 1595 2002 2049 3150
Size: 57 237 387 914 407 47 1101
Fragments arranged by size:
1101 914 407 387 237 57 47
PshAI GACnn'nnGTC
Cuts at: 0 715 3150
Size: 715 2435
PspOMI G'GGCC_C
Cuts at: 0 2025 2058 2301 2328 2374 2616 3150
Size: 2025 33 243 27 46 242 534
Fragments arranged by size:
2025 534 243 242 46 33 27
PstI C_TGCA'G
Cuts at: 0 359 974 1225 1346 1790 1838 2162 2383
Size: 359 615 251 121 444 48 324 221
Cuts at: 2383 2428 2636 2873 3053 3150
Size: 45 208 237 180 97
Fragments arranged by size:
615 444 359 324 251 237 221 208
180 121 97 48 45
PvuII CAG'CTG
Cuts at: 0 759 969 2191 2631 2697 3150
Size: 759 210 1222 440 66 453
Fragments arranged by size:
1222 759 453 440 210 66
SacI G_AGCT'C
Cuts at: 0 1162 2405 3150
Size: 1162 1243 745
Fragments arranged by size:
1243 1162 745
SacII CC_GC'GG
Cuts at: 0 15 3150
Size: 15 3135
SanDI GG'GwC_CC
Cuts at: 0 2002 3150
Size: 2002 1148
SapI GCTCTTCn'nnn_
Cuts at: 0 355 2355 3150
Size: 355 2000 795
Fragments arranged by size:
2000 795 355
SbfI CC_TGCA'GG
Cuts at: 0 2162 3150
Size: 2162 988
ScaI AGT'ACT
Cuts at: 0 280 3150
Size: 280 2870
SexAI A'CCwGG_T
Cuts at: 0 266 1100 2363 3150
Size: 266 834 1263 787
Fragments arranged by size:
1263 834 787 266
SfcI C'TryA_G
Cuts at: 0 355 970 1221 1342 1786 1834 2158 2379
Size: 355 615 251 121 444 48 324 221
Cuts at: 2379 2424 2632 2869 3049 3150
Size: 45 208 237 180 101
Fragments arranged by size:
615 444 355 324 251 237 221 208
180 121 101 48 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1253 2066 3150
Size: 1253 813 1084
Fragments arranged by size:
1253 1084 813
SfoI GGC'GCC
Cuts at: 0 75 372 656 1115 1281 3150
Size: 75 297 284 459 166 1869
Fragments arranged by size:
1869 459 297 284 166 75
SmaI CCC'GGG
Cuts at: 0 733 1024 2141 3150
Size: 733 291 1117 1009
Fragments arranged by size:
1117 1009 733 291
SmlI C'TyrA_G
Cuts at: 0 1517 2107 3150
Size: 1517 590 1043
Fragments arranged by size:
1517 1043 590
StyI C'CwwG_G
Cuts at: 0 1874 2010 2054 2171 3150
Size: 1874 136 44 117 979
Fragments arranged by size:
1874 979 136 117 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 837 1361 1365 1369 1477 1830 2228 3150
Size: 837 524 4 4 108 353 398 922
Fragments arranged by size:
922 837 524 398 353 108 4 4
TatI w'GTAC_w
Cuts at: 0 278 557 2825 3150
Size: 278 279 2268 325
Fragments arranged by size:
2268 325 279 278
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 922 954 1497 1529 3150
Size: 922 32 543 32 1621
Fragments arranged by size:
1621 922 543 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1069 3150
Size: 1069 2081
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 930 1453 1728 2368 3150
Size: 930 523 275 640 782
Fragments arranged by size:
930 782 640 523 275
XmaI C'CCGG_G
Cuts at: 0 731 1022 2139 3150
Size: 731 291 1117 1011
Fragments arranged by size:
1117 1011 731 291
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AleI AlfI
AloI AlwNI ApaI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI FseI FspI FspAI HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SmaI SmlI
StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AflIII AgeI AhdI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BglII BmtI BplI BsaAI BsaBI BsiWI BsmBI BspEI
BspHI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI
DraI EcoRI EcoRV HincII HindIII HpaI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SalI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XhoI XmnI ZraI