(Circular) (Six-base) MAPSORT of: pFN21ASDA0717.seq Check: 5014 from: 1 to: 6910 pFN21ASDA0717 6910 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2670 2670 Size: 6910 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2619 2826 3858 4720 Size: 53 83 186 936 1083 207 1032 862 Cuts at: 4720 278 Size: 2468 Fragments arranged by size: 2468 1083 1032 936 862 207 186 83 53 AccI GT'mk_AC Cuts at: 1937 4203 4253 1937 Size: 2266 50 4594 Fragments arranged by size: 4594 2266 50 Acc65I G'GTAC_C Cuts at: 2388 3153 4032 4231 2388 Size: 765 879 199 5067 Fragments arranged by size: 5067 879 765 199 AclI AA'CG_TT Cuts at: 1563 5037 5410 1563 Size: 3474 373 3063 Fragments arranged by size: 3474 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4058 4973 6357 6625 1632 Size: 2426 915 1384 268 1917 Fragments arranged by size: 2426 1917 1384 915 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6713 AflIII A'CryG_T Cuts at: 1203 3060 3945 5809 6786 1203 Size: 1857 885 1864 977 1327 Fragments arranged by size: 1864 1857 1327 977 885 AgeI A'CCGG_T Cuts at: 5741 5741 Size: 6910 AhdI GACnn_n'nnGTC Cuts at: 5639 5639 Size: 6910 AleI CACnn'nnGTG Cuts at: 1166 2168 4027 1166 Size: 1002 1859 4049 Fragments arranged by size: 4049 1859 1002 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6910 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4127 4159 4127 Size: 32 6878 AlwNI CAG_nnn'CTG Cuts at: 2158 4653 6225 2158 Size: 2495 1572 2843 Fragments arranged by size: 2843 2495 1572 ApaI G_GGCC'C Cuts at: 2945 3297 2945 Size: 352 6558 ApaLI G'TGCA_C Cuts at: 4967 6123 4967 Size: 1156 5754 ApoI r'AATT_y Cuts at: 1493 4219 4445 4597 6706 1493 Size: 2726 226 152 2109 1697 Fragments arranged by size: 2726 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5464 160 Size: 872 4432 1606 Fragments arranged by size: 4432 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 AvaI C'yCGr_G Cuts at: 1870 1943 2229 2415 3386 3636 3963 4235 1870 Size: 73 286 186 971 250 327 272 4545 Fragments arranged by size: 4545 971 327 286 272 250 186 73 BamHI G'GATC_C Cuts at: 4240 4607 4240 Size: 367 6543 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2388 2713 2755 2960 3153 Size: 325 217 627 601 325 42 205 193 Cuts at: 3153 3512 3548 4032 4231 5686 618 Size: 359 36 484 199 1455 1842 Fragments arranged by size: 1842 1455 627 601 484 359 325 325 217 205 199 193 42 36 BanII G_rGCy'C Cuts at: 1391 2945 3092 3297 3632 4229 1391 Size: 1554 147 205 335 597 4072 Fragments arranged by size: 4072 1554 597 335 205 147 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3174 4146 928 Size: 971 1275 972 3692 Fragments arranged by size: 3692 1275 972 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5101 5135 1903 Size: 34 3164 34 3678 Fragments arranged by size: 3678 3164 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4802 6012 4802 Size: 1210 5700 BclI T'GATC_A Cuts at: 1548 2992 1548 Size: 1444 5466 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4117 5521 136 Size: 107 122 71 631 605 2445 1404 1525 Fragments arranged by size: 2445 1525 1404 631 605 122 107 71 BglII A'GATC_T Cuts at: 2318 2868 6905 2318 Size: 550 4037 2323 Fragments arranged by size: 4037 2323 550 BlpI GC'TnA_GC Cuts at: 3378 4327 3378 Size: 949 5961 Bme1580I G_kGCm'C Cuts at: 1790 2122 2175 2760 2789 2945 2963 3297 4066 Size: 332 53 585 29 156 18 334 769 Cuts at: 4066 4971 6127 1790 Size: 905 1156 2573 Fragments arranged by size: 2573 1156 905 769 585 334 332 156 53 29 18 BmgBI CAC'GTC Cuts at: 1318 2236 3061 3472 1318 Size: 918 825 411 4756 Fragments arranged by size: 4756 918 825 411 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 2593 4641 Size: 416 53 83 399 24 723 453 2048 Cuts at: 4641 5599 442 Size: 958 1753 Fragments arranged by size: 2048 1753 958 723 453 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6910 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2660 2660 Size: 6910 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2489 2755 2822 3208 3700 5570 1239 Size: 1250 266 67 386 492 1870 2579 Fragments arranged by size: 2579 1870 1250 492 386 266 67 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3296 4085 5035 5900 6198 3296 Size: 789 950 865 298 4008 Fragments arranged by size: 4008 950 865 789 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2054 2277 2576 5573 882 Size: 617 555 223 299 2997 2219 Fragments arranged by size: 2997 2219 617 555 299 223 BsaAI yAC'GTr Cuts at: 493 1204 4207 4701 493 Size: 711 3003 494 2702 Fragments arranged by size: 3003 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6904 6904 Size: 6910 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2616 2823 3855 4717 Size: 53 83 186 936 1083 207 1032 862 Cuts at: 4717 5099 5734 275 Size: 382 635 1451 Fragments arranged by size: 1451 1083 1032 936 862 635 382 207 186 83 53 BsaWI w'CCGG_w Cuts at: 3092 5343 5741 6015 6162 6573 3092 Size: 2251 398 274 147 411 3429 Fragments arranged by size: 3429 2251 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2753 2783 3632 3662 1178 Size: 30 165 30 1350 30 849 30 4426 Fragments arranged by size: 4426 1350 849 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2795 2875 2878 4159 4162 4180 1186 Size: 1609 80 3 1281 3 18 3916 Fragments arranged by size: 3916 1609 1281 80 18 3 3 BseYI C'CCAG_C Cuts at: 2563 2935 3083 6113 6609 2563 Size: 372 148 3030 496 2864 Fragments arranged by size: 3030 2864 496 372 148 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2625 2714 3913 1163 Size: 573 889 89 1199 4160 Fragments arranged by size: 4160 1199 889 573 89 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4360 5121 6149 1540 Size: 275 186 2359 761 1028 2301 Fragments arranged by size: 2359 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2422 3632 4971 5056 6127 2422 Size: 1210 1339 85 1071 3205 Fragments arranged by size: 3205 1339 1210 1071 85 BsmI GAATG_Cn' Cuts at: 4421 4514 4421 Size: 93 6817 BsmBI CGTCTCn'nnnn_ Cuts at: 2226 2260 2226 Size: 34 6876 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2175 2422 2760 2789 2945 2963 Size: 399 332 53 247 338 29 156 18 Cuts at: 2963 3092 3297 3632 4066 4229 4971 5056 6127 Size: 129 205 335 434 163 742 85 1071 Cuts at: 6127 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 434 399 338 335 332 247 205 163 156 129 85 53 29 18 BspEI T'CCGG_A Cuts at: 6573 6573 Size: 6910 BspHI T'CATG_A Cuts at: 3441 4799 6529 3441 Size: 1358 1730 3822 Fragments arranged by size: 3822 1730 1358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2460 2670 4265 844 Size: 1616 210 1595 3489 Fragments arranged by size: 3489 1616 1595 210 BsrBI CCG'CTC Cuts at: 1394 1823 2812 2845 4797 1394 Size: 429 989 33 1952 3507 Fragments arranged by size: 3507 1952 989 429 33 BsrDI GCAATG_nn' Cuts at: 65 1248 2137 2346 2695 3363 3372 5405 5579 Size: 1183 889 209 349 668 9 2033 174 Cuts at: 5579 65 Size: 1396 Fragments arranged by size: 2033 1396 1183 889 668 349 209 174 9 BsrFI r'CCGG_y Cuts at: 1700 1810 3701 5554 5741 1700 Size: 110 1891 1853 187 2869 Fragments arranged by size: 2869 1891 1853 187 110 BsrGI T'GTAC_A Cuts at: 96 3321 96 Size: 3225 3685 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6910 BssSI C'ACGA_G Cuts at: 4970 5982 4970 Size: 1012 5898 BstAPI GCAn_nnn'nTGC Cuts at: 794 3669 794 Size: 2875 4035 BstBI TT'CG_AA Cuts at: 2007 5714 2007 Size: 3707 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6910 BstXI CCAn_nnnn'nTGG Cuts at: 2563 2593 3023 4029 2563 Size: 30 430 1006 5444 Fragments arranged by size: 5444 1006 430 30 BstYI r'GATC_y Cuts at: 1972 2318 2868 3129 3229 4240 4607 4992 5009 Size: 346 550 261 100 1011 367 385 17 Cuts at: 5009 6461 6547 6559 6905 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1011 550 385 367 346 346 261 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2094 3022 513 Size: 552 957 72 928 4401 Fragments arranged by size: 4401 957 928 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6171 Fragments arranged by size: 6171 683 56 BtsI GCAGTG_nn' Cuts at: 4426 5220 5240 4426 Size: 794 20 6096 Fragments arranged by size: 6096 794 20 ClaI AT'CG_AT Cuts at: 1554 3846 1554 Size: 2292 4618 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6875 DraI TTT'AAA Cuts at: 4214 4214 Size: 6910 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5917 809 Size: 507 4601 1802 Fragments arranged by size: 4601 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2160 2553 3414 Size: 54 1425 50 275 18 330 393 861 Cuts at: 3414 3903 4019 4357 5246 6875 8 Size: 489 116 338 889 1629 43 Fragments arranged by size: 1629 1425 889 861 489 393 338 330 275 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4357 1537 Size: 275 2545 4090 Fragments arranged by size: 4090 2545 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3210 4133 4840 1353 Size: 1857 923 707 3423 Fragments arranged by size: 3423 1857 923 707 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3464 5497 5871 6017 387 Size: 1064 138 1875 2033 374 146 1280 Fragments arranged by size: 2033 1875 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3630 3630 Size: 6910 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2489 2755 2822 3208 3700 4058 4973 Size: 393 857 266 67 386 492 358 915 Cuts at: 4973 5570 6357 6625 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 915 857 787 597 492 393 386 358 268 266 67 EcoNI CCTnn'n_nnAGG Cuts at: 2500 2801 2924 4040 2500 Size: 301 123 1116 5370 Fragments arranged by size: 5370 1116 301 123 EcoO109I rG'GnC_Cy Cuts at: 2806 2941 2942 3293 3294 2806 Size: 135 1 351 1 6422 Fragments arranged by size: 6422 351 135 1 1 EcoRI G'AATT_C Cuts at: 4219 4597 4219 Size: 378 6532 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2635 3517 2635 Size: 882 6028 FspI TGC'GCA Cuts at: 2852 3404 5416 2852 Size: 552 2012 4346 Fragments arranged by size: 4346 2012 552 FspAI rTGC'GCAy Cuts at: 2852 2852 Size: 6910 HaeII r_GCGC'y Cuts at: 1678 3561 6057 1678 Size: 1883 2496 2531 Fragments arranged by size: 2531 2496 1883 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2409 2441 2753 2785 5551 Size: 32 135 32 837 32 312 32 2766 Cuts at: 5551 5583 5625 5657 1373 Size: 32 42 32 2626 Fragments arranged by size: 2766 2626 837 312 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2479 4254 4500 669 Size: 988 281 541 1775 246 3079 Fragments arranged by size: 3079 1775 988 541 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6910 HpaI GTT'AAC Cuts at: 4500 4500 Size: 6910 KpnI G_GTAC'C Cuts at: 2392 3157 4036 4235 2392 Size: 765 879 199 5067 Fragments arranged by size: 5067 879 765 199 MfeI C'AATT_G Cuts at: 4509 4509 Size: 6910 MluI A'CGCG_T Cuts at: 6786 6786 Size: 6910 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2427 6024 6208 1305 Size: 1122 3597 184 2007 Fragments arranged by size: 3597 2007 1122 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2162 2555 3416 4021 10 Size: 54 1425 343 330 393 861 605 2899 Fragments arranged by size: 2899 1425 861 605 393 343 330 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2432 2753 2831 3021 3410 3704 Size: 648 1002 264 321 78 190 389 294 Cuts at: 3704 4027 4868 5227 5386 518 Size: 323 841 359 159 2042 Fragments arranged by size: 2042 1002 841 648 389 359 323 321 294 264 190 159 78 MspA1I CmG'CkG Cuts at: 1606 2155 2698 3585 3901 4060 4326 5003 6151 Size: 549 543 887 316 159 266 677 1148 Cuts at: 6151 6396 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 887 677 549 543 316 266 245 159 NcoI C'CATG_G Cuts at: 513 1065 2022 3022 513 Size: 552 957 1000 4401 Fragments arranged by size: 4401 1000 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6910 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6910 NotI GC'GGCC_GC Cuts at: 4357 4357 Size: 6910 NspI r_CATG'y Cuts at: 1132 1229 2171 3676 3709 4268 5813 1132 Size: 97 942 1505 33 559 1545 2229 Fragments arranged by size: 2229 1545 1505 942 559 97 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6910 PciI A'CATG_T Cuts at: 5809 5809 Size: 6910 PflMI CCAn_nnn'nTGG Cuts at: 1108 2158 2181 2800 3835 1108 Size: 1050 23 619 1035 4183 Fragments arranged by size: 4183 1050 1035 619 23 PfoI T'CCnGG_A Cuts at: 2799 3185 3210 3537 3867 2799 Size: 386 25 327 330 5842 Fragments arranged by size: 5842 386 330 327 25 PmeI GTTT'AAAC Cuts at: 4214 4214 Size: 6910 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4979 5011 6492 6524 4979 Size: 32 1481 32 5365 Fragments arranged by size: 5365 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2806 2806 Size: 6910 PshAI GACnn'nnGTC Cuts at: 1613 3912 1613 Size: 2299 4611 PsiI TTA'TAA Cuts at: 4480 4480 Size: 6910 PspOMI G'GGCC_C Cuts at: 2941 3293 2941 Size: 352 6558 PspXI vC'TCGA_Gb Cuts at: 3386 3386 Size: 6910 PstI C_TGCA'G Cuts at: 830 2667 2712 2841 4262 830 Size: 1837 45 129 1421 3478 Fragments arranged by size: 3478 1837 1421 129 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6910 PvuII CAG'CTG Cuts at: 2155 3585 3901 2155 Size: 1430 316 5164 Fragments arranged by size: 5164 1430 316 SacI G_AGCT'C Cuts at: 3632 3632 Size: 6910 SalI G'TCGA_C Cuts at: 1936 4252 1936 Size: 2316 4594 SapI GCTCTTCn'nnn_ Cuts at: 4133 4133 Size: 6910 SbfI CC_TGCA'GG Cuts at: 4262 4262 Size: 6910 ScaI AGT'ACT Cuts at: 5158 6870 5158 Size: 1712 5198 SexAI A'CCwGG_T Cuts at: 3777 3777 Size: 6910 SfcI C'TryA_G Cuts at: 826 1045 2663 2708 2837 4258 5393 6074 6265 Size: 219 1618 45 129 1421 1135 681 191 Cuts at: 6265 826 Size: 1471 Fragments arranged by size: 1618 1471 1421 1135 681 219 191 129 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6910 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6910 SmaI CCC'GGG Cuts at: 1872 3638 4237 1872 Size: 1766 599 4545 Fragments arranged by size: 4545 1766 599 SmlI C'TyrA_G Cuts at: 820 1017 1943 3311 3386 4100 5014 5915 6177 Size: 197 926 1368 75 714 914 901 262 Cuts at: 6177 820 Size: 1553 Fragments arranged by size: 1553 1368 926 914 901 714 262 197 75 SnaBI TAC'GTA Cuts at: 493 4207 4701 493 Size: 3714 494 2702 Fragments arranged by size: 3714 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6910 SphI G_CATG'C Cuts at: 1132 3676 4268 1132 Size: 2544 592 3774 Fragments arranged by size: 3774 2544 592 SspI AAT'ATT Cuts at: 5 52 4834 5 Size: 47 4782 2081 Fragments arranged by size: 4782 2081 47 StuI AGG'CCT Cuts at: 1517 3437 3464 1517 Size: 1920 27 4963 Fragments arranged by size: 4963 1920 27 StyI C'CwwG_G Cuts at: 513 1065 2022 2496 2556 3022 3459 4349 513 Size: 552 957 474 60 466 437 890 3074 Fragments arranged by size: 3074 957 890 552 474 466 437 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2978 2982 4937 4954 5107 5292 Size: 1255 579 819 4 1955 17 153 185 Cuts at: 5292 5737 325 Size: 445 1498 Fragments arranged by size: 1955 1498 1255 819 579 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3321 3798 5156 Size: 274 80 33 51 1443 1344 477 1358 Cuts at: 5156 6868 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1358 1344 477 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4060 4092 6870 6902 4060 Size: 32 2778 32 4068 Fragments arranged by size: 4068 2778 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6910 XbaI T'CTAG_A Cuts at: 4246 4246 Size: 6910 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3020 3640 3724 3020 Size: 620 84 6206 Fragments arranged by size: 6206 620 84 XhoI C'TCGA_G Cuts at: 1943 3386 1943 Size: 1443 5467 XmaI C'CCGG_G Cuts at: 1870 3636 4235 1870 Size: 1766 599 4545 Fragments arranged by size: 4545 1766 599 XmnI GAAnn'nnTTC Cuts at: 5039 5039 Size: 6910 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2617 2824 3856 4718 Size: 53 83 186 936 1083 207 1032 862 Cuts at: 4718 276 Size: 2468 Fragments arranged by size: 2468 1083 1032 936 862 207 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI