Restriction map for
pFN21ASDA0712
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# NO CUT ENZYMES ( 80 enzymes) #
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AatII Acc65I AclI AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
BbeI BbrPI BfrI BglI BlpI
Bpu1102I BsaAI BsaHI BsiWI BsmBI
BspDI BspLU11I BsrFI BssSI BstBI
BstEII BstPI CelII Cfr10I ClaI
CpoI DraI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FseI
Hin1I HpaI KasI KpnI KspI
MluI NaeI NarI NgoMIV NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 3145 CGCG CG^CG
AhdI 3019 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 3019 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3162 TGCGCA TGC^GCA
AvrII 4033 CCTAGG C^CTAGG
BamHI 2337 GGATCC G^GATCC
BcgI 3608 GCAGCCGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2484 TGATCA T^GATCA
BlnI 4033 CCTAGG C^CTAGG
BsiEI 2253 CGATCG CG[GA][CT]^CG
Bsp1407I 4055 TGTACA T^GTACA
BspHI 1218 TCATGA T^CATGA
BsrBI 3837 CCGCTC CCGCTC(-3/-3)
BsrDI 3314 CATTGC GCAATG(2/0)
BsrGI 4055 TGTACA T^GTACA
BssHII 3144 GCGCGC G^CGCGC
Bst1107I 2157 GTATAC GTA^TAC
BstAPI 1153 GCACTGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3145 CGCG CG^CG
BstZ17I 2157 GTATAC GTA^TAC
DraIII 3502 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 5004 GACTGTTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3019 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2484 TGATCA T^GATCA
FspI 3162 TGCGCA TGC^GCA
HgaI 3025 GCGTC GACGC(5/10)
HincII 1294 GTTGAC GT[CT]^[GA]AC
HindII 1294 GTTGAC GT[CT]^[GA]AC
HindIII 4549 AAGCTT A^AGCTT
MvnI 3145 CGCG CG^CG
NheI 2085 GCTAGC G^CTAGC
PaeR7I 3005 CTCGAG C^TCGAG
PshAI 458 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2253 CGATCG CGAT^CG
RcaI 1218 TCATGA T^CATGA
SacI 3636 GAGCTC GAGCT^C
SapI 1116 GCTCTTC GCTCTTC(1/4)
SexAI 1886 ACCAGGT A^CC[AT]GGT
SspBI 4055 TGTACA T^GTACA
XbaI 2229 TCTAGA T^CTAGA
XhoI 3005 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 2157 GTATAC GT^[AC][GT]AC
2386 GTAGAC
AccIII 3692 TCCGGA T^CCGGA
4217 TCCGGA
ApaI 3947 GGGCCC GGGCC^C
4633 GGGCCC
Asp700I 2447 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3667 GAAGACATTC
BalI 671 TGGCCA TGG^CCA
4656 TGGCCA
BanI 3118 GGTGCC G^G[CT][GA]CC
3514 GGTGCC
BbvCI 5050 CCTCAGC CCTCAGC(-5/-2)
1285 GCTGAGG
BciVI 1420 GGATAC GTATCC(6/5)
721 GTATCC
BglII 1138 AGATCT A^GATCT
2890 AGATCT
BsaBI 390 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1333 GATTTTGATC
BsaI 2133 GAGACC GGTCTC(1/5)
3987 GAGACC
BsaWI 3692 TCCGGA [AT]^CCGG[AT]
4217 TCCGGA
BseAI 3692 TCCGGA T^CCGGA
4217 TCCGGA
BsmI 1749 GAATGC GAATGC(1/-1)
133 GCATTC
BspEI 3692 TCCGGA T^CCGGA
4217 TCCGGA
HaeII 2082 GGCGCT [GA]GCGC^[CT]
4988 AGCGCC
MamI 390 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1333 GATTTTGATC
MfeI 518 CAATTG C^AATTG
794 CAATTG
MluNI 671 TGGCCA TGG^CCA
4656 TGGCCA
MroI 3692 TCCGGA T^CCGGA
4217 TCCGGA
MscI 671 TGGCCA TGG^CCA
4656 TGGCCA
MunI 518 CAATTG C^AATTG
794 CAATTG
NdeI 1859 CATATG CA^TATG
3560 CATATG
SmaI 3122 CCCGGG CCC^GGG
4085 CCCGGG
XmaCI 3122 CCCGGG C^CCGGG
4085 CCCGGG
XmaI 3122 CCCGGG C^CCGGG
4085 CCCGGG
XmnI 2447 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3667 GAAGACATTC
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII