Restriction map for
pFN21ASDA0696
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BseAI BseRI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FseI HaeII HgaI Hin1I
HindIII KasI KpnI KspI MamI
MfeI MflI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I Van91I XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AflIII 1479 ACGTGT A^C[GA][CT]GT
AhdI 241 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 241 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1427 CTCGAG C^[CT]CG[GA]G
BalI 1080 TGGCCA TGG^CCA
BanI 1297 GGTGCC G^G[CT][GA]CC
BcnI 1086 CCGGG CC^[GC]GG
BsaWI 1346 TCCGGT [AT]^CCGG[AT]
BsgI 434 GTGCAG GTGCAG(16/14)
BsiEI 1049 CGACCG CG[GA][CT]^CG
BsmBI 174 CGTCTC CGTCTC(1/5)
BsmI 482 GAATGC GAATGC(1/-1)
BsoBI 1427 CTCGAG C^[CT]CG[GA]G
BssSI 1167 CACGAG CACGAG(-5/-1)
BstAPI 117 GCAGAGCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 999 GGTGACC G^GT[ACGT]ACC
BstPI 999 GGTGACC G^GT[ACGT]ACC
DraI 574 TTTAAA TTT^AAA
DraII 1375 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1443 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1080 TGGCCA [CT]^GGCC[GA]
Eam1105I 241 GACCAGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 1375 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 999 GGTGACC G^GT[ACGT]ACC
EcoT22I 1341 ATGCAT ATGCA^T
HincII 825 GTTAAC GT[CT]^[GA]AC
HindII 825 GTTAAC GT[CT]^[GA]AC
HpaI 825 GTTAAC GTT^AAC
MaeII 1479 ACGT A^CGT
MluNI 1080 TGGCCA TGG^CCA
MscI 1080 TGGCCA TGG^CCA
MspA1I 346 CAGCGG C[AC]G^C[GT]G
NciI 1086 CCGGG CC^[GC]GG
NsiI 1341 ATGCAT ATGCA^T
PaeR7I 1427 CTCGAG C^TCGAG
SacI 37 GAGCTC GAGCT^C
SapI 887 GCTCTTC GCTCTTC(1/4)
SexAI 83 ACCTGGT A^CC[AT]GGT
SmlI 1427 CTCGAG C^T[CT][GA]AG
SphI 122 GCATGC GCATG^C
SspI 233 AATATT AAT^ATT
XbaI 1621 TCTAGA T^CTAGA
XhoI 1427 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 727 GTCTAC GT^[AC][GT]AC
1105 GTAGAC
AlwNI 565 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
839 CAGGCTCTG
Asp700I 397 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1180 GAATTTGTTC
AviII 100 TGCGCA TGC^GCA
1454 TGCGCA
BsaI 1585 GAGACC GGTCTC(1/5)
425 GGTCTC
BsaJI 55 CCAAGG C^C[ACGT][ACGT]GG
1010 CCAAGG
BsrBI 705 CCGCTC CCGCTC(-3/-3)
1017 CCGCTC
EarI 888 CTCTTC CTCTTC(1/4)
551 GAAGAG
EcoT14I 55 CCAAGG C^C[AT][AT]GG
1010 CCAAGG
FauI 348 GCGGG CCCGC(4/6)
1209 GCGGG
FspI 100 TGCGCA TGC^GCA
1454 TGCGCA
HapII 1086 CCGG C^CGG
1347 CCGG
HpaII 1086 CCGG C^CGG
1347 CCGG
Ksp632I 888 CTCTTC CTCTTC(1/4)
551 GAAGAG
MspI 1086 CCGG C^CGG
1347 CCGG
NspI 95 GCATGT [GA]CATG^[CT]
122 GCATGC
PleI 10 GACTC GAGTC(4/5)
1310 GAGTC
PstI 115 CTGCAG CTGCA^G
856 CTGCAG
StyI 55 CCAAGG C^C[AT][AT]GG
1010 CCAAGG
TaqI 35 TCGA T^CGA
1428 TCGA
Tsp45I 1000 GTGAC ^GT[GC]AC
1142 GTGAC
TthHB8I 35 TCGA T^CGA
1428 TCGA
XcmI 55 CCAAGGTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
144 CCAGATAAGTAATGG
XmnI 397 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1180 GAATTTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TfiI Tru9I TseI Tsp509I
TspRI