(Circular) (Six-base) MAPSORT of: pFN21ASDA0696.seq Check: 7579 from: 1 to: 6355 pFN21ASDA0696 6355 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2751 3129 3698 1937 Size: 814 378 569 4594 Fragments arranged by size: 4594 814 569 378 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 2898 2989 4418 5802 6070 1632 Size: 743 523 91 1429 1384 268 1917 Fragments arranged by size: 1917 1429 1384 743 523 268 91 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 3502 5254 6231 1203 Size: 2299 1752 977 1327 Fragments arranged by size: 2299 1752 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 2269 5084 2269 Size: 2815 3540 AleI CACnn'nnGTG Cuts at: 1166 2317 3485 1166 Size: 1151 1168 4036 Fragments arranged by size: 4036 1168 1151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2149 1184 Size: 965 5390 AlwNI CAG_nnn'CTG Cuts at: 2593 2867 4098 5670 2593 Size: 274 1231 1572 3278 Fragments arranged by size: 3278 1572 1231 274 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2291 2626 3203 3664 3890 4042 6151 1493 Size: 798 335 577 461 226 152 2109 1697 Fragments arranged by size: 2109 1697 798 577 461 335 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3450 3680 1870 Size: 73 1507 230 4545 Fragments arranged by size: 4545 1507 230 73 BamHI G'GATC_C Cuts at: 3685 4052 3685 Size: 367 5988 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3320 3676 5131 618 Size: 325 217 627 1533 356 1455 1842 Fragments arranged by size: 1842 1533 1455 627 356 325 217 BanII G_rGCy'C Cuts at: 1391 2031 2064 3457 3674 1391 Size: 640 33 1393 217 4072 Fragments arranged by size: 4072 1393 640 217 33 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5384 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4546 4580 1903 Size: 34 2609 34 3678 Fragments arranged by size: 3678 2609 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BfrBI ATG'CAT Cuts at: 3366 3366 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4966 136 Size: 107 122 71 631 605 3294 1525 Fragments arranged by size: 3294 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 3228 3601 4416 5572 1790 Size: 1438 373 815 1156 2573 Fragments arranged by size: 2573 1438 1156 815 373 BmgBI CAC'GTC Cuts at: 1318 3503 1318 Size: 2185 4170 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 3588 4086 Size: 416 53 83 399 24 728 1443 498 Cuts at: 4086 5044 442 Size: 958 1753 Fragments arranged by size: 1753 1443 958 728 498 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2050 2050 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 2185 3205 3233 3500 3607 5015 1239 Size: 911 35 1020 28 267 107 1408 2579 Fragments arranged by size: 2579 1408 1020 911 267 107 35 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2454 3602 5018 882 Size: 617 955 1148 1416 2219 Fragments arranged by size: 2219 1416 1148 955 617 BsaAI yAC'GTr Cuts at: 493 1204 3652 4146 493 Size: 711 2448 494 2702 Fragments arranged by size: 2702 2448 711 494 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4162 4544 5179 275 Size: 53 83 186 936 2629 382 635 1451 Fragments arranged by size: 2629 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3369 4788 5186 5460 5607 6018 3369 Size: 1419 398 274 147 411 3706 Fragments arranged by size: 3706 1419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3601 3631 1178 Size: 30 165 30 2198 30 3902 Fragments arranged by size: 3902 2198 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6355 BseYI C'CCAG_C Cuts at: 3457 5558 6054 3457 Size: 2101 496 3758 Fragments arranged by size: 3758 2101 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2478 1163 Size: 573 742 5040 Fragments arranged by size: 5040 742 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3075 3805 4566 5594 1540 Size: 275 186 1074 730 761 1028 2301 Fragments arranged by size: 2301 1074 1028 761 730 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 3191 3197 4416 4501 5572 2064 Size: 1127 6 1219 85 1071 2847 Fragments arranged by size: 2847 1219 1127 1071 85 6 BsmI GAATG_Cn' Cuts at: 2511 3866 3959 2511 Size: 1355 93 4907 Fragments arranged by size: 4907 1355 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2203 2203 Size: 6355 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2064 3191 3197 3228 3457 3601 Size: 399 241 33 1127 6 31 229 144 Cuts at: 3601 3674 4416 4501 5572 1391 Size: 73 742 85 1071 2174 Fragments arranged by size: 2174 1127 1071 742 399 241 229 144 85 73 33 31 6 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3710 844 Size: 2866 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2730 3042 4242 1394 Size: 429 907 312 1200 3507 Fragments arranged by size: 3507 1200 907 429 312 BsrDI GCAATG_nn' Cuts at: 65 1248 3016 3045 3237 4850 5024 65 Size: 1183 1768 29 192 1613 174 1396 Fragments arranged by size: 1768 1613 1396 1183 192 174 29 BsrFI r'CCGG_y Cuts at: 1700 1810 4999 5186 1700 Size: 110 3189 187 2869 Fragments arranged by size: 3189 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 3190 4415 5427 3190 Size: 1225 1012 4118 Fragments arranged by size: 4118 1225 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2146 794 Size: 1352 5003 BstBI TT'CG_AA Cuts at: 2007 5159 2007 Size: 3152 3203 BstEII G'GTnAC_C Cuts at: 1184 3022 1184 Size: 1838 4517 BstYI r'GATC_y Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350 Size: 1713 367 385 17 1452 86 12 346 Cuts at: 6350 1972 Size: 1977 Fragments arranged by size: 1977 1713 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4846 Fragments arranged by size: 4846 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 3871 4665 4685 3871 Size: 794 20 5541 Fragments arranged by size: 5541 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6320 DraI TTT'AAA Cuts at: 2599 3659 2599 Size: 1060 5295 DraIII CAC_nnn'GTG Cuts at: 3471 3471 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5362 809 Size: 507 4046 1802 Fragments arranged by size: 4046 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3103 3802 4691 Size: 54 1425 50 275 18 1273 699 889 Cuts at: 4691 6320 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1273 889 699 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2569 2917 4285 1353 Size: 1216 348 1368 3423 Fragments arranged by size: 3423 1368 1216 348 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4942 5316 5462 387 Size: 1064 138 3353 374 146 1280 Fragments arranged by size: 3353 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 2062 Size: 6355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2150 2185 2375 2898 2989 3205 3233 Size: 393 518 35 190 523 91 216 28 Cuts at: 3233 3500 3607 4418 5015 5802 6070 1239 Size: 267 107 811 597 787 268 1524 Fragments arranged by size: 1524 811 787 597 523 518 393 268 267 216 190 107 91 35 28 EcoO109I rG'GnC_Cy Cuts at: 3399 3399 Size: 6355 EcoRI G'AATT_C Cuts at: 3664 4042 3664 Size: 378 5977 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2525 3458 2525 Size: 933 5422 FspI TGC'GCA Cuts at: 2125 3479 4861 2125 Size: 1354 1382 3619 Fragments arranged by size: 3619 1382 1354 FspAI rTGC'GCAy Cuts at: 2125 2125 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 5502 1678 Size: 3824 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2255 2287 2432 2464 3601 Size: 32 135 32 683 32 145 32 1137 Cuts at: 3601 3633 4996 5028 5070 5102 1373 Size: 32 1363 32 42 32 2626 Fragments arranged by size: 2626 1363 1137 683 145 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2850 3699 3945 669 Size: 988 281 912 849 246 3079 Fragments arranged by size: 3079 988 912 849 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6355 HpaI GTT'AAC Cuts at: 2850 3945 2850 Size: 1095 5260 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5469 5653 1305 Size: 4164 184 2007 Fragments arranged by size: 4164 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3105 10 Size: 54 1425 343 1273 3260 Fragments arranged by size: 3260 1425 1273 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2317 2979 3050 3485 4313 4672 4831 Size: 648 1151 662 71 435 828 359 159 Cuts at: 4831 518 Size: 2042 Fragments arranged by size: 2042 1151 828 662 648 435 359 159 71 MspA1I CmG'CkG Cuts at: 1606 2371 3771 4448 5596 5841 1606 Size: 765 1400 677 1148 245 2120 Fragments arranged by size: 2120 1400 1148 765 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4846 Fragments arranged by size: 4846 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NsiI A_TGCA'T Cuts at: 3368 3368 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2122 2149 3713 5258 1132 Size: 97 893 27 1564 1545 2229 Fragments arranged by size: 2229 1564 1545 893 97 27 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6355 PfoI T'CCnGG_A Cuts at: 2661 2661 Size: 6355 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6355 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PspXI vC'TCGA_Gb Cuts at: 3450 3450 Size: 6355 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2520 2552 2520 Size: 32 6323 PstI C_TGCA'G Cuts at: 830 2142 2883 3707 830 Size: 1312 741 824 3478 Fragments arranged by size: 3478 1312 824 741 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 SacI G_AGCT'C Cuts at: 2064 2064 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SapI GCTCTTCn'nnn_ Cuts at: 2917 2917 Size: 6355 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SexAI A'CCwGG_T Cuts at: 2106 2106 Size: 6355 SfcI C'TryA_G Cuts at: 826 1045 2138 2676 2879 3703 4838 5519 5710 Size: 219 1093 538 203 824 1135 681 191 Cuts at: 5710 826 Size: 1471 Fragments arranged by size: 1471 1135 1093 824 681 538 219 203 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3450 4459 5360 5622 820 Size: 197 926 1507 1009 901 262 1553 Fragments arranged by size: 1553 1507 1009 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3652 4146 493 Size: 3159 494 2702 Fragments arranged by size: 3159 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 2149 3713 1132 Size: 1017 1564 3774 Fragments arranged by size: 3774 1564 1017 SspI AAT'ATT Cuts at: 5 52 2258 4279 5 Size: 47 2206 2021 2081 Fragments arranged by size: 2206 2081 2021 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6355 StyI C'CwwG_G Cuts at: 513 1065 2022 2078 3033 3794 513 Size: 552 957 56 955 761 3074 Fragments arranged by size: 3074 957 955 761 552 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3089 4382 4399 4552 4737 5182 325 Size: 1255 1509 1293 17 153 185 445 1498 Fragments arranged by size: 1509 1498 1293 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3144 3252 4601 Size: 274 80 33 51 1443 1167 108 1349 Cuts at: 4601 6313 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1349 1167 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3006 3038 3379 3411 6315 6347 3006 Size: 32 341 32 2904 32 3014 Fragments arranged by size: 3014 2904 341 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3644 3691 3644 Size: 47 6308 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2085 2174 2085 Size: 89 6266 XhoI C'TCGA_G Cuts at: 1943 3450 1943 Size: 1507 4848 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 2424 3207 4484 2424 Size: 783 1277 4295 Fragments arranged by size: 4295 1277 783 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI