Restriction map for
pFN21ASDA0696
(Linear) (Six-base) MAPSORT of: KIAA0696.seq Check: 2946 from: 1 to: 1626
KIAA0696.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 728 1106 1626
Size: 728 378 520
Fragments arranged by size:
728 520 378
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 875 966 1626
Size: 352 523 91 660
Fragments arranged by size:
660 523 352 91
AflIII A'CryG_T
Cuts at: 0 1479 1626
Size: 1479 147
AhdI GACnn_n'nnGTC
Cuts at: 0 246 1626
Size: 246 1380
AleI CACnn'nnGTG
Cuts at: 0 294 1462 1626
Size: 294 1168 164
Fragments arranged by size:
1168 294 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 126 1626
Size: 126 1500
AlwNI CAG_nnn'CTG
Cuts at: 0 570 844 1626
Size: 570 274 782
Fragments arranged by size:
782 570 274
ApoI r'AATT_y
Cuts at: 0 268 603 1180 1626
Size: 268 335 577 446
Fragments arranged by size:
577 446 335 268
AvaI C'yCGr_G
Cuts at: 0 1427 1626
Size: 1427 199
BanI G'GyrC_C
Cuts at: 0 1297 1626
Size: 1297 329
BanII G_rGCy'C
Cuts at: 0 8 41 1434 1626
Size: 8 33 1393 192
Fragments arranged by size:
1393 192 33 8
BfrBI ATG'CAT
Cuts at: 0 1343 1626
Size: 1343 283
Bme1580I G_kGCm'C
Cuts at: 0 1205 1578 1626
Size: 1205 373 48
Fragments arranged by size:
1205 373 48
BmgBI CAC'GTC
Cuts at: 0 1480 1626
Size: 1480 146
BmrI ACTGGGnnnn_n'
Cuts at: 0 122 1565 1626
Size: 122 1443 61
Fragments arranged by size:
1443 122 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1626
Size: 27 1599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 162 1182 1210 1477 1584 1626
Size: 127 35 1020 28 267 107 42
Fragments arranged by size:
1020 267 127 107 42 35 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 431 1579 1626
Size: 431 1148 47
Fragments arranged by size:
1148 431 47
BsaWI w'CCGG_w
Cuts at: 0 1346 1626
Size: 1346 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1578 1608 1626
Size: 1578 30 18
Fragments arranged by size:
1578 30 18
BseYI C'CCAG_C
Cuts at: 0 1434 1626
Size: 1434 192
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1626
Size: 455 1171
BsiEI CG_ry'CG
Cuts at: 0 1052 1626
Size: 1052 574
BsiHKAI G_wGCw'C
Cuts at: 0 41 1168 1174 1626
Size: 41 1127 6 452
Fragments arranged by size:
1127 452 41 6
BsmI GAATG_Cn'
Cuts at: 0 488 1626
Size: 488 1138
BsmBI CGTCTCn'nnnn_
Cuts at: 0 180 1626
Size: 180 1446
Bsp1286I G_dGCh'C
Cuts at: 0 8 41 1168 1174 1205 1434 1578 1626
Size: 8 33 1127 6 31 229 144 48
Fragments arranged by size:
1127 229 144 48 33 31 8 6
BsrBI CCG'CTC
Cuts at: 0 707 1019 1626
Size: 707 312 607
Fragments arranged by size:
707 607 312
BsrDI GCAATG_nn'
Cuts at: 0 993 1022 1214 1626
Size: 993 29 192 412
Fragments arranged by size:
993 412 192 29
BssSI C'ACGA_G
Cuts at: 0 1167 1626
Size: 1167 459
BstAPI GCAn_nnn'nTGC
Cuts at: 0 123 1626
Size: 123 1503
BstEII G'GTnAC_C
Cuts at: 0 999 1626
Size: 999 627
DraI TTT'AAA
Cuts at: 0 576 1626
Size: 576 1050
DraIII CAC_nnn'GTG
Cuts at: 0 1448 1626
Size: 1448 178
EaeI y'GGCC_r
Cuts at: 0 1080 1626
Size: 1080 546
EarI CTCTTCn'nnn_
Cuts at: 0 546 894 1626
Size: 546 348 732
Fragments arranged by size:
732 546 348
EcoICRI GAG'CTC
Cuts at: 0 39 1626
Size: 39 1587
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 162 352 875 966 1182 1210 1477
Size: 127 35 190 523 91 216 28 267
Cuts at: 1477 1584 1626
Size: 107 42
Fragments arranged by size:
523 267 216 190 127 107 91 42
35 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1376 1626
Size: 1376 250
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 502 1435 1626
Size: 502 933 191
Fragments arranged by size:
933 502 191
FspI TGC'GCA
Cuts at: 0 102 1456 1626
Size: 102 1354 170
Fragments arranged by size:
1354 170 102
FspAI rTGC'GCAy
Cuts at: 0 102 1626
Size: 102 1524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 232 264 409 441 1578 1610 1626
Size: 232 32 145 32 1137 32 16
Fragments arranged by size:
1137 232 145 32 32 32 16
HincII GTy'rAC
Cuts at: 0 827 1626
Size: 827 799
HpaI GTT'AAC
Cuts at: 0 827 1626
Size: 827 799
MscI TGG'CCA
Cuts at: 0 1082 1626
Size: 1082 544
MslI CAynn'nnrTG
Cuts at: 0 294 956 1027 1462 1626
Size: 294 662 71 435 164
Fragments arranged by size:
662 435 294 164 71
MspA1I CmG'CkG
Cuts at: 0 348 1626
Size: 348 1278
NsiI A_TGCA'T
Cuts at: 0 1345 1626
Size: 1345 281
NspI r_CATG'y
Cuts at: 0 99 126 1626
Size: 99 27 1500
Fragments arranged by size:
1500 99 27
PfoI T'CCnGG_A
Cuts at: 0 638 1626
Size: 638 988
PspXI vC'TCGA_Gb
Cuts at: 0 1427 1626
Size: 1427 199
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 497 529 1626
Size: 497 32 1097
Fragments arranged by size:
1097 497 32
PstI C_TGCA'G
Cuts at: 0 119 860 1626
Size: 119 741 766
Fragments arranged by size:
766 741 119
SacI G_AGCT'C
Cuts at: 0 41 1626
Size: 41 1585
SapI GCTCTTCn'nnn_
Cuts at: 0 894 1626
Size: 894 732
SexAI A'CCwGG_T
Cuts at: 0 83 1626
Size: 83 1543
SfcI C'TryA_G
Cuts at: 0 115 653 856 1626
Size: 115 538 203 770
Fragments arranged by size:
770 538 203 115
SmlI C'TyrA_G
Cuts at: 0 1427 1626
Size: 1427 199
SphI G_CATG'C
Cuts at: 0 126 1626
Size: 126 1500
SspI AAT'ATT
Cuts at: 0 235 1626
Size: 235 1391
StyI C'CwwG_G
Cuts at: 0 55 1010 1626
Size: 55 955 616
Fragments arranged by size:
955 616 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1066 1626
Size: 1066 560
TatI w'GTAC_w
Cuts at: 0 1121 1229 1626
Size: 1121 108 397
Fragments arranged by size:
1121 397 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1356 1388 1626
Size: 983 32 341 32 238
Fragments arranged by size:
983 341 238 32 32
XbaI T'CTAG_A
Cuts at: 0 1621 1626
Size: 1621 5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 62 151 1626
Size: 62 89 1475
Fragments arranged by size:
1475 89 62
XhoI C'TCGA_G
Cuts at: 0 1427 1626
Size: 1427 199
XmnI GAAnn'nnTTC
Cuts at: 0 401 1184 1626
Size: 401 783 442
Fragments arranged by size:
783 442 401
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApoI
AvaI BanI BanII BfrBI Bme1580I BmgBI BmrI BplI
BpmI BsaI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BsrBI BsrDI BssSI BstAPI BstEII
DraI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I FalI
FspI FspAI Hin4I HincII HpaI MscI MslI MspA1I
NsiI NspI PfoI PspXI PsrI PstI SacI SapI
SexAI SfcI SmlI SphI SspI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI BmtI Bpu10I BpuEI BsaAI BsaBI BsaHI BseRI
BsiWI BspEI BspHI BspMI BsrFI BsrGI BssHII BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DrdI EagI EciI EcoNI EcoRI EcoRV FseI
HaeII HindIII KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI Tth111I XmaI ZraI