Restriction map for
pFN21ASDA0694
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflII AflIII AgeI AhdI Alw44I
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BbeI
BbrPI BbvCI BcgI BciVI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EcoNI EcoO65I EcoRI FseI FspI
Hin1I HpaI KasI KpnI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI Tsp45I
Tth111I Van91I XcmI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
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AclI 1754 AACGTT AA^CGTT
AlwNI 284 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 877 CCTGAGG CC^T[ACGT]AGG
Aor51HI 623 AGCGCT AGC^GCT
ApaI 883 GGGCCC GGGCC^C
AvaII 14 GGACC G^G[AT]CC
AvrII 133 CCTAGG C^CTAGG
BalI 1523 TGGCCA TGG^CCA
BamHI 842 GGATCC G^GATCC
BanI 1564 GGTGCC G^G[CT][GA]CC
BbsI 507 GTCTTC GAAGAC(2/6)
BcnI 29 CCCGG CC^[GC]GG
BglI 88 GCCGCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 133 CCTAGG C^CTAGG
BpmI 392 CTGGAG CTGGAG(16/14)
BpuAI 507 GTCTTC GAAGAC(2/6)
BsaI 42 GAGACC GGTCTC(1/5)
BsaWI 191 ACCGGA [AT]^CCGG[AT]
BsgI 742 GTGCAG GTGCAG(16/14)
BsiEI 56 CGGCCG CG[GA][CT]^CG
BsmFI 1443 GGGAC GGGAC(10/14)
Bsp1407I 730 TGTACA T^GTACA
BspHI 1181 TCATGA T^CATGA
BspMI 20 GCAGGT ACCTGC(4/8)
BsrDI 1554 CATTGC GCAATG(2/0)
BsrGI 730 TGTACA T^GTACA
BstXI 1450 CCAGAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 877 CCTGAGG CC^T[ACGT]AGG
DsaI 89 CCGCGG C^C[GA][CT]GG
EagI 56 CGGCCG C^GGCCG
EarI 203 CTCTTC CTCTTC(1/4)
EclXI 56 CGGCCG C^GGCCG
Eco47III 623 AGCGCT AGC^GCT
Eco52I 56 CGGCCG C^GGCCG
Eco81I 877 CCTGAGG CC^T[ACGT]AGG
EcoRV 262 GATATC GAT^ATC
EcoT22I 711 ATGCAT ATGCA^T
HgaI 79 GCGTC GACGC(5/10)
HincII 1690 GTTGAC GT[CT]^[GA]AC
HindII 1690 GTTGAC GT[CT]^[GA]AC
Ksp632I 203 CTCTTC CTCTTC(1/4)
KspI 89 CCGCGG CCGC^GG
MluNI 1523 TGGCCA TGG^CCA
MscI 1523 TGGCCA TGG^CCA
MslI 466 CATGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 107 GCCGGC GCC^GGC
NciI 29 CCCGG CC^[GC]GG
NgoMIV 107 GCCGGC G^CCGGC
NsiI 711 ATGCAT ATGCA^T
NspI 360 GCATGT [GA]CATG^[CT]
PaeR7I 142 CTCGAG C^TCGAG
Psp1406I 1754 AACGTT AA^CGTT
PstI 1638 CTGCAG CTGCA^G
RcaI 1181 TCATGA T^CATGA
SacII 89 CCGCGG CCGC^GG
SfcI 1638 CTGCAG C^T[GA][CT]AG
SmlI 142 CTCGAG C^T[CT][GA]AG
SspBI 730 TGTACA T^GTACA
XbaI 1083 TCTAGA T^CTAGA
XhoI 142 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 78 CGCG CG^CG
90 CGCG
AvaI 142 CTCGAG C^[CT]CG[GA]G
413 CCCGAG
BclI 1145 TGATCA T^GATCA
1184 TGATCA
BglII 210 AGATCT A^GATCT
1122 AGATCT
BsmI 958 GAATGC GAATGC(1/-1)
860 GCATTC
BsoBI 142 CTCGAG C^[CT]CG[GA]G
413 CCCGAG
BsrBI 10 GAGCGG CCGCTC(-3/-3)
527 GAGCGG
BsrFI 4 GCCGGT [GA]^CCGG[CT]
107 GCCGGC
BstAPI 1003 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1558 GCAAGTGGTGC
BstNI 46 CCTGG CC^[AT]GG
257 CCTGG
BstUI 78 CGCG CG^CG
90 CGCG
CfoI 74 GCGC GCG^C
624 GCGC
Cfr10I 4 GCCGGT [GA]^CCGG[CT]
107 GCCGGC
DraII 882 GGGGCCC [GA]G^G[ACGT]CC[CT]
883 GGGCCCC
EcoO109I 882 GGGGCCC [GA]G^G[ACGT]CC[CT]
883 GGGCCCC
EcoRII 46 CCTGG ^CC[AT]GG
257 CCTGG
FauI 17 CCCGC CCCGC(4/6)
529 GCGGG
FbaI 1145 TGATCA T^GATCA
1184 TGATCA
HaeII 73 AGCGCC [GA]GCGC^[CT]
623 AGCGCT
HhaI 74 GCGC GCG^C
624 GCGC
HinP1I 74 GCGC G^CGC
624 GCGC
HindIII 436 AAGCTT A^AGCTT
1740 AAGCTT
HphI 7 GGTGA GGTGA(8/7)
26 TCACC
MaeII 271 ACGT A^CGT
1755 ACGT
MvaI 46 CCTGG CC^[AT]GG
257 CCTGG
MvnI 78 CGCG CG^CG
90 CGCG
PvuII 634 CAGCTG CAG^CTG
812 CAGCTG
SacI 61 GAGCTC GAGCT^C
910 GAGCTC
SspI 386 AATATT AAT^ATT
1255 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI BanII BbvI BfaI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII