(Circular) (Six-base) MAPSORT of: pFN21ASDA0694.seq Check: 4930 from: 1 to: 6508 pFN21ASDA0694 6508 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3851 1937 Size: 1914 4594 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 3778 4635 5008 1563 Size: 2215 857 373 3063 Fragments arranged by size: 3063 2215 857 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 2991 3221 3751 4571 5955 6223 1632 Size: 702 657 230 530 820 1384 268 1917 Fragments arranged by size: 1917 1384 820 702 657 530 268 230 AfeI AGC'GCT Cuts at: 2648 2648 Size: 6508 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 5407 6384 1203 Size: 4204 977 1327 Fragments arranged by size: 4204 1327 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6508 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3599 3631 3599 Size: 32 6476 AlwNI CAG_nnn'CTG Cuts at: 2312 4251 5823 2312 Size: 1939 1572 2997 Fragments arranged by size: 2997 1939 1572 ApaI G_GGCC'C Cuts at: 2910 2910 Size: 6508 ApaLI G'TGCA_C Cuts at: 4565 5721 4565 Size: 1156 5352 ApoI r'AATT_y Cuts at: 1493 2338 2363 2596 3488 3817 4043 4195 6304 Size: 845 25 233 892 329 226 152 2109 Cuts at: 6304 1493 Size: 1697 Fragments arranged by size: 2109 1697 892 845 329 233 226 152 25 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 2165 2436 3833 1870 Size: 73 222 271 1397 4545 Fragments arranged by size: 4545 1397 271 222 73 AvrII C'CTAG_G Cuts at: 2156 2156 Size: 6508 BamHI G'GATC_C Cuts at: 2865 3838 4205 2865 Size: 973 367 5168 Fragments arranged by size: 5168 973 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3587 3829 5284 618 Size: 325 217 627 1800 242 1455 1842 Fragments arranged by size: 1842 1800 1455 627 325 242 217 BanII G_rGCy'C Cuts at: 1391 2088 2910 2937 3827 1391 Size: 697 822 27 890 4072 Fragments arranged by size: 4072 890 822 697 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2523 928 Size: 971 624 4913 Fragments arranged by size: 4913 971 624 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 3168 3207 1548 Size: 1620 39 4849 Fragments arranged by size: 4849 1620 39 BfrBI ATG'CAT Cuts at: 2736 2736 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2117 5119 136 Size: 107 122 71 631 605 445 3002 1525 Fragments arranged by size: 3002 1525 631 605 445 122 107 71 BglII A'GATC_T Cuts at: 2233 3145 6503 2233 Size: 912 3358 2238 Fragments arranged by size: 3358 2238 912 BlpI GC'TnA_GC Cuts at: 3925 3925 Size: 6508 Bme1580I G_kGCm'C Cuts at: 1790 2910 4569 5725 1790 Size: 1120 1659 1156 2573 Fragments arranged by size: 2573 1659 1156 1120 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2244 2371 4239 Size: 416 53 83 399 24 827 127 1868 Cuts at: 4239 5197 442 Size: 958 1753 Fragments arranged by size: 1868 1753 958 827 416 399 127 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2436 5168 1239 Size: 1197 2732 2579 Fragments arranged by size: 2732 2579 1197 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4633 5498 5796 4633 Size: 865 298 5345 Fragments arranged by size: 5345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2059 5171 882 Size: 617 560 3112 2219 Fragments arranged by size: 3112 2219 617 560 BsaAI yAC'GTr Cuts at: 493 1204 3805 4299 493 Size: 711 2601 494 2702 Fragments arranged by size: 2702 2601 711 494 BsaBI GATnn'nnATC Cuts at: 6502 6502 Size: 6508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4315 4697 5332 275 Size: 53 83 186 936 2782 382 635 1451 Fragments arranged by size: 2782 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2214 4941 5339 5613 5760 6171 2214 Size: 2727 398 274 147 411 2551 Fragments arranged by size: 2727 2551 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3418 3448 3599 3629 1178 Size: 30 165 30 2015 30 151 30 4057 Fragments arranged by size: 4057 2015 165 151 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2562 2935 2938 1186 Size: 1376 373 3 4756 Fragments arranged by size: 4756 1376 373 3 BseYI C'CCAG_C Cuts at: 5711 6207 5711 Size: 496 6012 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2786 1163 Size: 573 1050 4885 Fragments arranged by size: 4885 1050 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 3958 4719 5747 1540 Size: 275 186 81 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 81 BsiHKAI G_wGCw'C Cuts at: 2088 2328 2930 2937 4569 4654 5725 2088 Size: 240 602 7 1632 85 1071 2871 Fragments arranged by size: 2871 1632 1071 602 240 85 7 BsmI GAATG_Cn' Cuts at: 2883 2987 4019 4112 2883 Size: 104 1032 93 5279 Fragments arranged by size: 5279 1032 104 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2328 2910 2930 2937 3827 4569 Size: 399 298 240 582 20 7 890 742 Cuts at: 4569 4654 5725 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 890 742 582 399 298 240 85 20 7 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 3204 4397 6127 3204 Size: 1193 1730 3585 Fragments arranged by size: 3585 1730 1193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2034 3863 844 Size: 1190 1829 3489 Fragments arranged by size: 3489 1829 1190 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2552 4395 1394 Size: 429 212 517 1843 3507 Fragments arranged by size: 3507 1843 517 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 3576 5003 5177 65 Size: 1183 2328 1427 174 1396 Fragments arranged by size: 2328 1427 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 2130 5152 5339 1700 Size: 110 217 103 3022 187 2869 Fragments arranged by size: 3022 2869 217 187 110 103 BsrGI T'GTAC_A Cuts at: 96 2753 96 Size: 2657 3851 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 4568 5580 4568 Size: 1012 5496 BstAPI GCAn_nnn'nTGC Cuts at: 794 3032 3587 794 Size: 2238 555 3715 Fragments arranged by size: 3715 2238 555 BstBI TT'CG_AA Cuts at: 2007 5312 2007 Size: 3305 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6508 BstXI CCAn_nnnn'nTGG Cuts at: 3480 3480 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2233 2865 3109 3145 3355 3838 4205 4590 Size: 261 632 244 36 210 483 367 385 Cuts at: 4590 4607 6059 6145 6157 6503 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 632 483 385 367 346 261 244 210 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2901 2901 Size: 6508 BtgI C'CryG_G Cuts at: 513 1065 2022 2112 513 Size: 552 957 90 4909 Fragments arranged by size: 4909 957 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 4024 4818 4838 4024 Size: 794 20 5694 Fragments arranged by size: 5694 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2524 2559 529 Size: 35 1960 35 4478 Fragments arranged by size: 4478 1960 35 35 DraI TTT'AAA Cuts at: 3812 3812 Size: 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2079 3546 Size: 54 1425 50 275 18 195 54 1467 Cuts at: 3546 3955 4844 6473 8 Size: 409 889 1629 43 Fragments arranged by size: 1629 1467 1425 889 409 275 195 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2079 3955 1537 Size: 275 267 1876 4090 Fragments arranged by size: 4090 1876 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 2232 4438 1353 Size: 879 2206 3423 Fragments arranged by size: 3423 2206 879 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2095 5095 5469 5615 387 Size: 1064 138 506 3000 374 146 1280 Fragments arranged by size: 3000 1280 1064 506 374 146 138 EcoICRI GAG'CTC Cuts at: 2086 2935 2086 Size: 849 5659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2334 2436 2991 3221 3751 4571 5168 Size: 393 702 102 555 230 530 820 597 Cuts at: 5168 5955 6223 1239 Size: 787 268 1524 Fragments arranged by size: 1524 820 787 702 597 555 530 393 268 230 102 EcoO109I rG'GnC_Cy Cuts at: 2906 2907 2906 Size: 1 6507 EcoRI G'AATT_C Cuts at: 3817 4195 3817 Size: 378 6130 EcoRV GAT'ATC Cuts at: 2287 2287 Size: 6508 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2668 2668 Size: 6508 FspI TGC'GCA Cuts at: 5014 5014 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 2100 2650 5655 1678 Size: 422 550 3005 2531 Fragments arranged by size: 3005 2531 550 422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2699 2731 3079 3111 3369 Size: 32 135 32 1127 32 348 32 258 Cuts at: 3369 3401 5149 5181 5223 5255 1373 Size: 32 1748 32 42 32 2626 Fragments arranged by size: 2626 1748 1127 348 258 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3715 3852 4098 669 Size: 988 281 1777 137 246 3079 Fragments arranged by size: 3079 1777 988 281 246 137 HindIII A'AGCT_T Cuts at: 748 2459 3763 748 Size: 1711 1304 3493 Fragments arranged by size: 3493 1711 1304 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 4107 4107 Size: 6508 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3642 3672 5622 5806 1305 Size: 2337 30 1950 184 2007 Fragments arranged by size: 2337 2007 1950 184 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 3548 10 Size: 54 1425 343 1716 2970 Fragments arranged by size: 2970 1716 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2493 4466 4825 4984 518 Size: 648 1327 1973 359 159 2042 Fragments arranged by size: 2042 1973 1327 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2114 2143 2659 2837 3924 4601 5749 5994 Size: 508 29 516 178 1087 677 1148 245 Cuts at: 5994 1606 Size: 2120 Fragments arranged by size: 2120 1148 1087 677 516 508 245 178 29 NaeI GCC'GGC Cuts at: 2132 2132 Size: 6508 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4999 Fragments arranged by size: 4999 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NgoMIV G'CCGG_C Cuts at: 2130 2130 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NsiI A_TGCA'T Cuts at: 2738 2738 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 2387 3866 5411 1132 Size: 97 1158 1479 1545 2229 Fragments arranged by size: 2229 1545 1479 1158 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6508 PciI A'CATG_T Cuts at: 5407 5407 Size: 6508 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6508 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3599 3631 4577 4609 6090 6122 3599 Size: 32 946 32 1481 32 3985 Fragments arranged by size: 3985 1481 946 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6508 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PspOMI G'GGCC_C Cuts at: 2906 2906 Size: 6508 PstI C_TGCA'G Cuts at: 830 3665 3860 830 Size: 2835 195 3478 Fragments arranged by size: 3478 2835 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6508 PvuII CAG'CTG Cuts at: 2659 2837 2659 Size: 178 6330 SacI G_AGCT'C Cuts at: 2088 2937 2088 Size: 849 5659 SacII CC_GC'GG Cuts at: 2115 2115 Size: 6508 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 4756 6468 4756 Size: 1712 4796 SfcI C'TryA_G Cuts at: 826 1045 3661 3856 4991 5672 5863 826 Size: 219 2616 195 1135 681 191 1471 Fragments arranged by size: 2616 1471 1135 681 219 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3835 1872 Size: 1963 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 4612 5513 5775 820 Size: 197 926 222 2447 901 262 1553 Fragments arranged by size: 2447 1553 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 3805 4299 493 Size: 3312 494 2702 Fragments arranged by size: 3312 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 2411 3280 4432 5 Size: 47 2359 869 1152 2081 Fragments arranged by size: 2359 2081 1152 869 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6508 StyI C'CwwG_G Cuts at: 513 1065 2022 2156 2535 2914 3947 513 Size: 552 957 134 379 379 1033 3074 Fragments arranged by size: 3074 1033 957 552 379 379 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2622 4535 4552 4705 4890 5335 325 Size: 1255 1042 1913 17 153 185 445 1498 Fragments arranged by size: 1913 1498 1255 1042 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2298 2753 4754 Size: 274 80 33 51 1443 321 455 2001 Cuts at: 4754 6466 96 Size: 1712 138 Fragments arranged by size: 2001 1712 1443 455 321 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 3418 3450 6468 6500 2516 Size: 32 870 32 3018 32 2524 Fragments arranged by size: 3018 2524 870 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6508 XbaI T'CTAG_A Cuts at: 3106 3844 3106 Size: 738 5770 XhoI C'TCGA_G Cuts at: 1943 2165 1943 Size: 222 6286 XmaI C'CCGG_G Cuts at: 1870 3833 1870 Size: 1963 4545 XmnI GAAnn'nnTTC Cuts at: 4637 4637 Size: 6508 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI FseI FspAI KasI NarI NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI