Restriction map for
pFN21ASDA0694
(Linear) (Six-base) MAPSORT of: KIAA0694.seq Check: 7721 from: 1 to: 1779
KIAA0694.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AclI AA'CG_TT
Cuts at: 0 1755 1779
Size: 1755 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 968 1198 1728 1779
Size: 311 657 230 530 51
Fragments arranged by size:
657 530 311 230 51
AfeI AGC'GCT
Cuts at: 0 625 1779
Size: 625 1154
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 1779
Size: 1576 32 171
Fragments arranged by size:
1576 171 32
AlwNI CAG_nnn'CTG
Cuts at: 0 289 1779
Size: 289 1490
ApaI G_GGCC'C
Cuts at: 0 887 1779
Size: 887 892
ApoI r'AATT_y
Cuts at: 0 315 340 573 1465 1779
Size: 315 25 233 892 314
Fragments arranged by size:
892 315 314 233 25
AvaI C'yCGr_G
Cuts at: 0 142 413 1779
Size: 142 271 1366
Fragments arranged by size:
1366 271 142
AvrII C'CTAG_G
Cuts at: 0 133 1779
Size: 133 1646
BamHI G'GATC_C
Cuts at: 0 842 1779
Size: 842 937
BanI G'GyrC_C
Cuts at: 0 1564 1779
Size: 1564 215
BanII G_rGCy'C
Cuts at: 0 65 887 914 1779
Size: 65 822 27 865
Fragments arranged by size:
865 822 65 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1779
Size: 500 1279
BclI T'GATC_A
Cuts at: 0 1145 1184 1779
Size: 1145 39 595
Fragments arranged by size:
1145 595 39
BfrBI ATG'CAT
Cuts at: 0 713 1779
Size: 713 1066
BglI GCCn_nnn'nGGC
Cuts at: 0 94 1779
Size: 94 1685
BglII A'GATC_T
Cuts at: 0 210 1122 1779
Size: 210 912 657
Fragments arranged by size:
912 657 210
Bme1580I G_kGCm'C
Cuts at: 0 887 1779
Size: 887 892
BmrI ACTGGGnnnn_n'
Cuts at: 0 221 348 1779
Size: 221 127 1431
Fragments arranged by size:
1431 221 127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1779
Size: 413 1366
BsaI GGTCTCn'nnnn_
Cuts at: 0 36 1779
Size: 36 1743
BsaWI w'CCGG_w
Cuts at: 0 191 1779
Size: 191 1588
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1395 1425 1576 1606 1779
Size: 1395 30 151 30 173
Fragments arranged by size:
1395 173 151 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 539 912 915 1779
Size: 539 373 3 864
Fragments arranged by size:
864 539 373 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 1779
Size: 763 1016
BsiEI CG_ry'CG
Cuts at: 0 59 1779
Size: 59 1720
BsiHKAI G_wGCw'C
Cuts at: 0 65 305 907 914 1779
Size: 65 240 602 7 865
Fragments arranged by size:
865 602 240 65 7
BsmI GAATG_Cn'
Cuts at: 0 860 964 1779
Size: 860 104 815
Fragments arranged by size:
860 815 104
Bsp1286I G_dGCh'C
Cuts at: 0 65 305 887 907 914 1779
Size: 65 240 582 20 7 865
Fragments arranged by size:
865 582 240 65 20 7
BspHI T'CATG_A
Cuts at: 0 1181 1779
Size: 1181 598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 11 1779
Size: 11 1768
BsrBI CCG'CTC
Cuts at: 0 12 529 1779
Size: 12 517 1250
Fragments arranged by size:
1250 517 12
BsrDI GCAATG_nn'
Cuts at: 0 1553 1779
Size: 1553 226
BsrFI r'CCGG_y
Cuts at: 0 4 107 1779
Size: 4 103 1672
Fragments arranged by size:
1672 103 4
BsrGI T'GTAC_A
Cuts at: 0 730 1779
Size: 730 1049
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1009 1564 1779
Size: 1009 555 215
Fragments arranged by size:
1009 555 215
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1457 1779
Size: 1457 322
BstYI r'GATC_y
Cuts at: 0 210 842 1086 1122 1332 1779
Size: 210 632 244 36 210 447
Fragments arranged by size:
632 447 244 210 210 36
Bsu36I CC'TnA_GG
Cuts at: 0 878 1779
Size: 878 901
BtgI C'CryG_G
Cuts at: 0 89 1779
Size: 89 1690
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 501 536 1779
Size: 501 35 1243
Fragments arranged by size:
1243 501 35
EaeI y'GGCC_r
Cuts at: 0 2 56 1523 1779
Size: 2 54 1467 256
Fragments arranged by size:
1467 256 54 2
EagI C'GGCC_G
Cuts at: 0 56 1779
Size: 56 1723
EarI CTCTTCn'nnn_
Cuts at: 0 209 1779
Size: 209 1570
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 72 1779
Size: 72 1707
EcoICRI GAG'CTC
Cuts at: 0 63 912 1779
Size: 63 849 867
Fragments arranged by size:
867 849 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 413 968 1198 1728 1779
Size: 311 102 555 230 530 51
Fragments arranged by size:
555 530 311 230 102 51
EcoO109I rG'GnC_Cy
Cuts at: 0 883 884 1779
Size: 883 1 895
Fragments arranged by size:
895 883 1
EcoRV GAT'ATC
Cuts at: 0 264 1779
Size: 264 1515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 645 1779
Size: 645 1134
HaeII r_GCGC'y
Cuts at: 0 77 627 1779
Size: 77 550 1152
Fragments arranged by size:
1152 550 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 1056 1088 1346 1378 1779
Size: 676 32 348 32 258 32 401
Fragments arranged by size:
676 401 348 258 32 32 32
HincII GTy'rAC
Cuts at: 0 1692 1779
Size: 1692 87
HindIII A'AGCT_T
Cuts at: 0 436 1740 1779
Size: 436 1304 39
Fragments arranged by size:
1304 436 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1619 1649 1779
Size: 1619 30 130
Fragments arranged by size:
1619 130 30
MscI TGG'CCA
Cuts at: 0 1525 1779
Size: 1525 254
MslI CAynn'nnrTG
Cuts at: 0 470 1779
Size: 470 1309
MspA1I CmG'CkG
Cuts at: 0 91 120 636 814 1779
Size: 91 29 516 178 965
Fragments arranged by size:
965 516 178 91 29
NaeI GCC'GGC
Cuts at: 0 109 1779
Size: 109 1670
NgoMIV G'CCGG_C
Cuts at: 0 107 1779
Size: 107 1672
NsiI A_TGCA'T
Cuts at: 0 715 1779
Size: 715 1064
NspI r_CATG'y
Cuts at: 0 364 1779
Size: 364 1415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 1779
Size: 1576 32 171
Fragments arranged by size:
1576 171 32
PspOMI G'GGCC_C
Cuts at: 0 883 1779
Size: 883 896
PstI C_TGCA'G
Cuts at: 0 1642 1779
Size: 1642 137
PvuII CAG'CTG
Cuts at: 0 636 814 1779
Size: 636 178 965
Fragments arranged by size:
965 636 178
SacI G_AGCT'C
Cuts at: 0 65 914 1779
Size: 65 849 865
Fragments arranged by size:
865 849 65
SacII CC_GC'GG
Cuts at: 0 92 1779
Size: 92 1687
SfcI C'TryA_G
Cuts at: 0 1638 1779
Size: 1638 141
SmlI C'TyrA_G
Cuts at: 0 142 1779
Size: 142 1637
SspI AAT'ATT
Cuts at: 0 388 1257 1779
Size: 388 869 522
Fragments arranged by size:
869 522 388
StyI C'CwwG_G
Cuts at: 0 133 512 891 1779
Size: 133 379 379 888
Fragments arranged by size:
888 379 379 133
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 599 1779
Size: 599 1180
TatI w'GTAC_w
Cuts at: 0 275 730 1779
Size: 275 455 1049
Fragments arranged by size:
1049 455 275
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 493 525 1395 1427 1779
Size: 493 32 870 32 352
Fragments arranged by size:
870 493 352 32 32
XbaI T'CTAG_A
Cuts at: 0 1083 1779
Size: 1083 696
XhoI C'TCGA_G
Cuts at: 0 142 1779
Size: 142 1637
Enzymes that do cut:
AclI AcuI AfeI AloI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbsI BclI BfrBI BglI
BglII Bme1580I BmrI BpmI BsaI BsaWI BsaXI BseRI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BstAPI BstXI BstYI Bsu36I BtgI
CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NgoMIV NsiI NspI PpiI PspOMI
PstI PvuII SacI SacII SfcI SmlI SspI StyI
TaqII TatI TstI XbaI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AflII AflIII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI BaeI BbeI
BbvCI BcgI BciVI BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BseYI BsiWI BsmBI BspEI
BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI
DraI DraIII DrdI EcoNI EcoRI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XcmI XmaI XmnI ZraI