(Circular) (Six-base) MAPSORT of: pFN21ASDA0690.seq Check: 1347 from: 1 to: 8320 pFN21ASDA0690 8320 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6130 278 Size: 53 83 186 936 4594 2468 Fragments arranged by size: 4594 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5608 5663 1937 Size: 3671 55 4594 Fragments arranged by size: 4594 3671 55 Acc65I G'GTAC_C Cuts at: 2300 5641 2300 Size: 3341 4979 AclI AA'CG_TT Cuts at: 1563 6447 6820 1563 Size: 4884 373 3063 Fragments arranged by size: 4884 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2326 2675 3071 3530 3783 4193 6383 Size: 499 195 349 396 459 253 410 2190 Cuts at: 6383 7767 8035 1632 Size: 1384 268 1917 Fragments arranged by size: 2190 1917 1384 499 459 410 396 349 268 253 195 AfeI AGC'GCT Cuts at: 2072 2357 2072 Size: 285 8035 AflII C'TTAA_G Cuts at: 820 1017 2894 820 Size: 197 1877 6246 Fragments arranged by size: 6246 1877 197 AflIII A'CryG_T Cuts at: 1203 4663 7219 8196 1203 Size: 3460 2556 977 1327 Fragments arranged by size: 3460 2556 1327 977 AgeI A'CCGG_T Cuts at: 7151 7151 Size: 8320 AhdI GACnn_n'nnGTC Cuts at: 7049 7049 Size: 8320 AleI CACnn'nnGTG Cuts at: 1166 2420 3577 1166 Size: 1254 1157 5909 Fragments arranged by size: 5909 1254 1157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8320 AlwNI CAG_nnn'CTG Cuts at: 3113 3202 3268 3715 3752 4552 4634 6063 7635 Size: 89 66 447 37 800 82 1429 1572 Cuts at: 7635 3113 Size: 3798 Fragments arranged by size: 3798 1572 1429 800 447 89 82 66 37 ApaI G_GGCC'C Cuts at: 4136 4144 4136 Size: 8 8312 ApaLI G'TGCA_C Cuts at: 4315 6377 7533 4315 Size: 2062 1156 5102 Fragments arranged by size: 5102 2062 1156 ApoI r'AATT_y Cuts at: 1493 2525 3167 5629 5855 6007 8116 1493 Size: 1032 642 2462 226 152 2109 1697 Fragments arranged by size: 2462 2109 1697 1032 642 226 152 AseI AT'TA_AT Cuts at: 160 1032 6874 160 Size: 872 5842 1606 Fragments arranged by size: 5842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8320 AvaI C'yCGr_G Cuts at: 1870 1943 2032 2296 4136 4796 4842 5645 1870 Size: 73 89 264 1840 660 46 803 4545 Fragments arranged by size: 4545 1840 803 660 264 89 73 46 BamHI G'GATC_C Cuts at: 5086 5467 5650 6017 5086 Size: 381 183 367 7389 Fragments arranged by size: 7389 381 367 183 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2300 4854 5231 5641 7096 Size: 325 217 627 513 2554 377 410 1455 Cuts at: 7096 618 Size: 1842 Fragments arranged by size: 2554 1842 1455 627 513 410 377 325 217 BanII G_rGCy'C Cuts at: 1391 2820 3188 4136 4144 4864 4917 5073 5639 Size: 1429 368 948 8 720 53 156 566 Cuts at: 5639 1391 Size: 4072 Fragments arranged by size: 4072 1429 948 720 566 368 156 53 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3202 4184 928 Size: 971 1303 982 5064 Fragments arranged by size: 5064 1303 982 971 BbvCI CC'TCA_GC Cuts at: 4836 5325 4836 Size: 489 7831 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6511 6545 1903 Size: 34 4574 34 3678 Fragments arranged by size: 4574 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6212 7422 6212 Size: 1210 7110 BclI T'GATC_A Cuts at: 1548 5184 5277 1548 Size: 3636 93 4591 Fragments arranged by size: 4591 3636 93 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2762 4455 5399 Size: 107 122 71 631 605 1090 1693 944 Cuts at: 5399 6931 136 Size: 1532 1525 Fragments arranged by size: 1693 1532 1525 1090 944 631 605 122 107 71 BglII A'GATC_T Cuts at: 2391 3561 8315 2391 Size: 1170 4754 2396 Fragments arranged by size: 4754 2396 1170 BlpI GC'TnA_GC Cuts at: 5737 5737 Size: 8320 Bme1580I G_kGCm'C Cuts at: 1790 4136 4144 4319 6381 7537 1790 Size: 2346 8 175 2062 1156 2573 Fragments arranged by size: 2573 2346 2062 1156 175 8 BmgBI CAC'GTC Cuts at: 1318 4594 4645 1318 Size: 3276 51 4993 Fragments arranged by size: 4993 3276 51 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 4619 6051 Size: 416 53 83 399 24 1436 1766 1432 Cuts at: 6051 7009 442 Size: 958 1753 Fragments arranged by size: 1766 1753 1436 1432 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2888 1056 Size: 1832 6488 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 2389 2311 Size: 78 8242 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2628 3202 3245 3560 3825 4127 4184 4574 Size: 1389 574 43 315 265 302 57 390 Cuts at: 4574 5360 5385 6980 1239 Size: 786 25 1595 2579 Fragments arranged by size: 2579 1595 1389 786 574 390 315 302 265 57 43 25 Bpu10I CC'TnA_GC Cuts at: 2798 3534 4836 4865 5325 2798 Size: 736 1302 29 460 5793 Fragments arranged by size: 5793 1302 736 460 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2498 2749 3023 5291 6445 7310 7608 2498 Size: 251 274 2268 1154 865 298 3210 Fragments arranged by size: 3210 2268 1154 865 298 274 251 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3438 6983 882 Size: 617 1939 3545 2219 Fragments arranged by size: 3545 2219 1939 617 BsaAI yAC'GTr Cuts at: 493 1204 5617 6111 493 Size: 711 4413 494 2702 Fragments arranged by size: 4413 2702 711 494 BsaBI GATnn'nnATC Cuts at: 8314 8314 Size: 8320 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2252 6127 6509 7144 Size: 53 83 186 936 719 3875 382 635 Cuts at: 7144 275 Size: 1451 Fragments arranged by size: 3875 1451 936 719 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2478 4827 6753 7151 7425 7572 7983 2478 Size: 2349 1926 398 274 147 411 2815 Fragments arranged by size: 2815 2349 1926 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 3071 3101 4100 Size: 30 165 30 1041 30 597 30 999 Cuts at: 4100 4130 4547 4577 1178 Size: 30 417 30 4921 Fragments arranged by size: 4921 1041 999 597 417 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2702 3088 3164 3413 4124 4198 4814 4898 Size: 1516 386 76 249 711 74 616 84 Cuts at: 4898 4910 4913 4916 4919 4922 4925 4958 4985 Size: 12 3 3 3 3 3 33 27 Cuts at: 4985 4997 5000 5047 5210 5342 5345 5348 1186 Size: 12 3 47 163 132 3 3 4158 Fragments arranged by size: 4158 1516 711 616 386 249 163 132 84 76 74 47 33 27 12 12 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2490 3661 7523 8019 2490 Size: 1171 3862 496 2791 Fragments arranged by size: 3862 2791 1171 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2311 2621 3595 4103 4172 4590 4688 Size: 573 575 310 974 508 69 418 98 Cuts at: 4688 1163 Size: 4795 Fragments arranged by size: 4795 974 575 573 508 418 310 98 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2156 5465 5770 6531 7559 1540 Size: 275 186 155 3309 305 761 1028 2301 Fragments arranged by size: 3309 2301 1028 761 305 275 186 155 BsiHKAI G_wGCw'C Cuts at: 2804 2820 3188 3945 4205 4319 6381 6466 7537 Size: 16 368 757 260 114 2062 85 1071 Cuts at: 7537 2804 Size: 3587 Fragments arranged by size: 3587 2062 1071 757 368 260 114 85 16 BsmI GAATG_Cn' Cuts at: 3062 5376 5831 5924 3062 Size: 2314 455 93 5458 Fragments arranged by size: 5458 2314 455 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2243 2441 2243 Size: 198 8122 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2804 2820 3188 3945 4136 4144 4205 Size: 399 1014 16 368 757 191 8 61 Cuts at: 4205 4319 4864 4917 5073 5639 6381 6466 7537 Size: 114 545 53 156 566 742 85 1071 Cuts at: 7537 1391 Size: 2174 Fragments arranged by size: 2174 1071 1014 757 742 566 545 399 368 191 156 114 85 61 53 16 8 BspEI T'CCGG_A Cuts at: 2478 4827 7983 2478 Size: 2349 3156 2815 Fragments arranged by size: 3156 2815 2349 BspHI T'CATG_A Cuts at: 2721 6209 7939 2721 Size: 3488 1730 3102 Fragments arranged by size: 3488 3102 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2670 2996 3285 5675 844 Size: 1826 326 289 2390 3489 Fragments arranged by size: 3489 2390 1826 326 289 BsrBI CCG'CTC Cuts at: 1394 1823 6207 1394 Size: 429 4384 3507 Fragments arranged by size: 4384 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6815 6989 65 Size: 1183 5567 174 1396 Fragments arranged by size: 5567 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2151 5178 5460 6964 7151 1700 Size: 110 341 3027 282 1504 187 2869 Fragments arranged by size: 3027 2869 1504 341 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8320 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8320 BssSI C'ACGA_G Cuts at: 4364 6380 7392 4364 Size: 2016 1012 5292 Fragments arranged by size: 5292 2016 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3373 794 Size: 2579 5741 BstBI TT'CG_AA Cuts at: 2007 7124 2007 Size: 5117 3203 BstEII G'GTnAC_C Cuts at: 1184 2983 3337 3697 4462 1184 Size: 1799 354 360 765 5042 Fragments arranged by size: 5042 1799 765 360 354 BstXI CCAn_nnnn'nTGG Cuts at: 3423 3529 4540 3423 Size: 106 1011 7203 Fragments arranged by size: 7203 1011 106 BstYI r'GATC_y Cuts at: 1972 2391 3045 3561 5086 5467 5650 6017 6402 Size: 419 654 516 1525 381 183 367 385 Cuts at: 6402 6419 7871 7957 7969 8315 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1525 1452 654 516 419 385 381 367 346 183 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2714 3106 3354 3692 4128 4388 2714 Size: 392 248 338 436 260 6646 Fragments arranged by size: 6646 436 392 338 260 248 BtgI C'CryG_G Cuts at: 513 1065 2022 2256 3416 3522 3822 4075 4467 Size: 552 957 234 1160 106 300 253 392 Cuts at: 4467 4878 513 Size: 411 3955 Fragments arranged by size: 3955 1160 957 552 411 392 300 253 234 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5366 487 Size: 683 56 4140 3441 Fragments arranged by size: 4140 3441 683 56 BtsI GCAGTG_nn' Cuts at: 2527 2571 2707 2942 3150 3330 4181 4287 4358 Size: 44 136 235 208 180 851 106 71 Cuts at: 4358 4427 4635 5836 6630 6650 2527 Size: 69 208 1201 794 20 4197 Fragments arranged by size: 4197 1201 851 794 235 208 208 180 136 106 71 69 44 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8320 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8285 DraI TTT'AAA Cuts at: 4516 5624 4516 Size: 1108 7212 DraIII CAC_nnn'GTG Cuts at: 3244 3705 4081 4252 3244 Size: 461 376 171 7312 Fragments arranged by size: 7312 461 376 171 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2177 3695 7327 809 Size: 507 861 1518 3632 1802 Fragments arranged by size: 3632 1802 1518 861 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2153 3630 3669 Size: 54 1425 50 275 18 323 1477 39 Cuts at: 3669 4656 5112 5176 5391 5462 5767 6656 8285 Size: 987 456 64 215 71 305 889 1629 Cuts at: 8285 8 Size: 43 Fragments arranged by size: 1629 1477 1425 987 889 456 323 305 275 215 71 64 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 2153 5462 5767 1537 Size: 275 341 3309 305 4090 Fragments arranged by size: 4090 3309 341 305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 2687 2783 3506 3806 4151 4404 4887 Size: 906 428 96 723 300 345 253 483 Cuts at: 4887 5217 5244 6250 1353 Size: 330 27 1006 3423 Fragments arranged by size: 3423 1006 906 723 483 428 345 330 300 253 96 27 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2285 6907 7281 7427 387 Size: 1064 138 696 4622 374 146 1280 Fragments arranged by size: 4622 1280 1064 696 374 146 138 EcoICRI GAG'CTC Cuts at: 2818 3186 2818 Size: 368 7952 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2131 2326 2628 2675 3071 3202 3245 Size: 393 499 195 302 47 396 131 43 Cuts at: 3245 3530 3560 3783 3825 4127 4184 4193 4574 Size: 285 30 223 42 302 57 9 381 Cuts at: 4574 5360 5385 6383 6980 7767 8035 1239 Size: 786 25 998 597 787 268 1524 Fragments arranged by size: 1524 998 787 786 597 499 396 393 381 302 302 285 268 223 195 131 57 47 43 42 30 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 3533 4868 5552 3533 Size: 1335 684 6301 Fragments arranged by size: 6301 1335 684 EcoO109I rG'GnC_Cy Cuts at: 2217 2542 2659 2941 3110 3154 3350 3712 4132 Size: 325 117 282 169 44 196 362 420 Cuts at: 4132 4133 4140 4141 4168 4224 4414 2217 Size: 1 7 1 27 56 190 6123 Fragments arranged by size: 6123 420 362 325 282 196 190 169 117 56 44 27 7 1 1 EcoRI G'AATT_C Cuts at: 2525 5629 6007 2525 Size: 3104 378 4838 Fragments arranged by size: 4838 3104 378 FseI GG_CCGG'CC Cuts at: 5464 5464 Size: 8320 FspI TGC'GCA Cuts at: 3719 6826 3719 Size: 3107 5213 HaeII r_GCGC'y Cuts at: 1678 2074 2359 5172 7467 1678 Size: 396 285 2813 2295 2531 Fragments arranged by size: 2813 2531 2295 396 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 3071 3103 3963 Size: 32 135 32 623 32 844 32 860 Cuts at: 3963 3995 4803 4835 5335 5367 6961 6993 7035 Size: 32 808 32 500 32 1594 32 42 Cuts at: 7035 7067 1373 Size: 32 2626 Fragments arranged by size: 2626 1594 860 844 808 623 500 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4822 5609 5664 5910 669 Size: 988 281 2884 787 55 246 3079 Fragments arranged by size: 3079 2884 988 787 281 246 55 HindIII A'AGCT_T Cuts at: 748 4694 4730 748 Size: 3946 36 4338 Fragments arranged by size: 4338 3946 36 HpaI GTT'AAC Cuts at: 5910 5910 Size: 8320 KpnI G_GTAC'C Cuts at: 2304 5645 2304 Size: 3341 4979 MfeI C'AATT_G Cuts at: 5919 5919 Size: 8320 MluI A'CGCG_T Cuts at: 8196 8196 Size: 8320 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 2394 7434 7618 1305 Size: 1041 48 5040 184 2007 Fragments arranged by size: 5040 2007 1041 184 48 MscI TGG'CCA Cuts at: 10 64 1489 1832 3632 3671 4658 5114 5393 Size: 54 1425 343 1800 39 987 456 279 Cuts at: 5393 10 Size: 2937 Fragments arranged by size: 2937 1800 1425 987 456 343 279 54 39 MslI CAynn'nnrTG Cuts at: 518 1166 2420 3577 4622 4643 6278 6637 6796 Size: 648 1254 1157 1045 21 1635 359 159 Cuts at: 6796 518 Size: 2042 Fragments arranged by size: 2042 1635 1254 1157 1045 648 359 159 21 MspA1I CmG'CkG Cuts at: 1606 3199 4268 4476 4549 4672 5014 5736 6413 Size: 1593 1069 208 73 123 342 722 677 Cuts at: 6413 7561 7806 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1593 1148 1069 722 677 342 245 208 123 73 NaeI GCC'GGC Cuts at: 2153 5180 5462 2153 Size: 3027 282 5011 Fragments arranged by size: 5011 3027 282 NcoI C'CATG_G Cuts at: 513 1065 2022 2256 3522 4467 513 Size: 552 957 234 1266 945 4366 Fragments arranged by size: 4366 1266 957 945 552 234 NdeI CA'TA_TG Cuts at: 387 387 Size: 8320 NgoMIV G'CCGG_C Cuts at: 2151 5178 5460 2151 Size: 3027 282 5011 Fragments arranged by size: 5011 3027 282 NheI G'CTAG_C Cuts at: 1052 2884 1052 Size: 1832 6488 NotI GC'GGCC_GC Cuts at: 5767 5767 Size: 8320 NspI r_CATG'y Cuts at: 1132 1229 2644 4667 4711 5678 7223 1132 Size: 97 1415 2023 44 967 1545 2229 Fragments arranged by size: 2229 2023 1545 1415 967 97 44 PasI CC'CwG_GG Cuts at: 1348 2434 4921 1348 Size: 1086 2487 4747 Fragments arranged by size: 4747 2487 1086 PciI A'CATG_T Cuts at: 4663 7219 4663 Size: 2556 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2740 3325 1108 Size: 1632 585 6103 Fragments arranged by size: 6103 1632 585 PfoI T'CCnGG_A Cuts at: 2589 2676 3918 3981 5082 2589 Size: 87 1242 63 1101 5827 Fragments arranged by size: 5827 1242 1101 87 63 PmeI GTTT'AAAC Cuts at: 5624 5624 Size: 8320 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3435 3467 6389 6421 7902 7934 3435 Size: 32 2922 32 1481 32 3821 Fragments arranged by size: 3821 2922 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2217 2659 2941 3350 4168 2217 Size: 442 282 409 818 6369 Fragments arranged by size: 6369 818 442 409 282 PshAI GACnn'nnGTC Cuts at: 1613 4469 1613 Size: 2856 5464 PsiI TTA'TAA Cuts at: 5890 5890 Size: 8320 PspOMI G'GGCC_C Cuts at: 4132 4140 4132 Size: 8 8312 PstI C_TGCA'G Cuts at: 830 2211 2702 3369 4422 4481 5532 5672 830 Size: 1381 491 667 1053 59 1051 140 3478 Fragments arranged by size: 3478 1381 1053 1051 667 491 140 59 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8320 PvuII CAG'CTG Cuts at: 3199 4268 4476 4549 4672 3199 Size: 1069 208 73 123 6847 Fragments arranged by size: 6847 1069 208 123 73 SacI G_AGCT'C Cuts at: 2820 3188 2820 Size: 368 7952 SalI G'TCGA_C Cuts at: 1936 5607 5662 1936 Size: 3671 55 4594 Fragments arranged by size: 4594 3671 55 SapI GCTCTTCn'nnn_ Cuts at: 2687 3506 2687 Size: 819 7501 SbfI CC_TGCA'GG Cuts at: 5672 5672 Size: 8320 ScaI AGT'ACT Cuts at: 2462 6568 8280 2462 Size: 4106 1712 2502 Fragments arranged by size: 4106 2502 1712 SexAI A'CCwGG_T Cuts at: 2922 3987 2922 Size: 1065 7255 SfcI C'TryA_G Cuts at: 826 1045 2207 2698 3365 3621 4418 4477 5528 Size: 219 1162 491 667 256 797 59 1051 Cuts at: 5528 5668 6803 7484 7675 826 Size: 140 1135 681 191 1471 Fragments arranged by size: 1471 1162 1135 1051 797 681 667 491 256 219 191 140 59 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8320 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8320 SmaI CCC'GGG Cuts at: 1872 4138 4844 5647 1872 Size: 2266 706 803 4545 Fragments arranged by size: 4545 2266 803 706 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 2728 2894 3038 5306 6424 Size: 197 926 570 215 166 144 2268 1118 Cuts at: 6424 7325 7587 820 Size: 901 262 1553 Fragments arranged by size: 2268 1553 1118 926 901 570 262 215 197 166 144 SnaBI TAC'GTA Cuts at: 493 5617 6111 493 Size: 5124 494 2702 Fragments arranged by size: 5124 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8320 SphI G_CATG'C Cuts at: 1132 5678 1132 Size: 4546 3774 SrfI GCCC'GGGC Cuts at: 4844 4844 Size: 8320 SspI AAT'ATT Cuts at: 5 52 6244 5 Size: 47 6192 2081 Fragments arranged by size: 6192 2081 47 StuI AGG'CCT Cuts at: 1517 2685 2765 2791 3619 4094 5547 1517 Size: 1168 80 26 828 475 1453 4290 Fragments arranged by size: 4290 1453 1168 828 475 80 26 StyI C'CwwG_G Cuts at: 513 1065 2022 2256 2616 3522 3732 3999 4320 Size: 552 957 234 360 906 210 267 321 Cuts at: 4320 4467 5091 5759 513 Size: 147 624 668 3074 Fragments arranged by size: 3074 957 906 668 624 552 360 321 267 234 210 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6347 6364 6517 6702 7147 325 Size: 1255 4767 17 153 185 445 1498 Fragments arranged by size: 4767 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2350 2460 4535 Size: 274 80 33 51 1443 373 110 2075 Cuts at: 4535 6566 8278 96 Size: 2031 1712 138 Fragments arranged by size: 2075 2031 1712 1443 373 274 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2967 2999 3342 3374 4100 4132 4547 4579 8280 Size: 32 343 32 726 32 415 32 3701 Cuts at: 8280 8312 2967 Size: 32 2975 Fragments arranged by size: 3701 2975 726 415 343 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3989 4595 1316 Size: 2673 606 5041 Fragments arranged by size: 5041 2673 606 XbaI T'CTAG_A Cuts at: 5656 5656 Size: 8320 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2789 2789 Size: 8320 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8320 XmaI C'CCGG_G Cuts at: 1870 4136 4842 5645 1870 Size: 2266 706 803 4545 Fragments arranged by size: 4545 2266 803 706 XmnI GAAnn'nnTTC Cuts at: 4742 6449 4742 Size: 1707 6613 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6128 276 Size: 53 83 186 936 4594 2468 Fragments arranged by size: 4594 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SwaI