Restriction map for
pFN21ASDA0685
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AviII BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BpuAI BsaAI BsaBI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco81I EcoRI
EcoRV FbaI FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 23 enzymes) #
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AatII 541 GACGTC GACGT^C
AspHI 1090 GAGCTC G[AT]GC[AT]^C
AvrII 2682 CCTAGG C^CTAGG
BfaI 2683 CTAG C^TAG
BlnI 2682 CCTAGG C^CTAGG
BlpI 306 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 306 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 1090 GAGCTC G[AT]GC[AT]^C
BspLU11I 761 ACATGT A^CATGT
BsrDI 393 CATTGC GCAATG(2/0)
BstAPI 2410 GCAGCGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1326 GGTGACC G^GT[ACGT]ACC
BstPI 1326 GGTGACC G^GT[ACGT]ACC
CelII 306 GCTGAGC GC^T[ACGT]AGC
EcoNI 1981 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1326 GGTGACC G^GT[ACGT]ACC
EcoT22I 1179 ATGCAT ATGCA^T
HincII 37 GTTGAC GT[CT]^[GA]AC
HindII 37 GTTGAC GT[CT]^[GA]AC
MaeI 2683 CTAG C^TAG
NsiI 1179 ATGCAT ATGCA^T
PpuMI 2439 GGGTCCC [GA]G^G[AT]CC[CT]
SacI 1090 GAGCTC GAGCT^C
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# TWO CUT ENZYMES ( 34 enzymes) #
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AccII 1455 CGCG CG^CG
2544 CGCG
AflIII 761 ACATGT A^C[GA][CT]GT
2324 ACGTGT
Aor51HI 480 AGCGCT AGC^GCT
720 AGCGCT
Asp700I 936 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2289 GAAAGCCTTC
BbeI 1036 GGCGCC GGCGC^C
2050 GGCGCC
BsaI 668 GAGACC GGTCTC(1/5)
773 GAGACC
BsmI 1020 GAATGC GAATGC(1/-1)
2248 GAATGC
BspMI 491 ACCTGC ACCTGC(4/8)
1987 GCAGGT
BsrBI 709 CCGCTC CCGCTC(-3/-3)
1474 GAGCGG
BstUI 1455 CGCG CG^CG
2544 CGCG
EagI 1298 CGGCCG C^GGCCG
2445 CGGCCG
EarI 2655 CTCTTC CTCTTC(1/4)
562 GAAGAG
EclXI 1298 CGGCCG C^GGCCG
2445 CGGCCG
Eco47III 480 AGCGCT AGC^GCT
720 AGCGCT
Eco52I 1298 CGGCCG C^GGCCG
2445 CGGCCG
KasI 1036 GGCGCC G^GCGCC
2050 GGCGCC
Ksp632I 2655 CTCTTC CTCTTC(1/4)
562 GAAGAG
MvnI 1455 CGCG CG^CG
2544 CGCG
NarI 1036 GGCGCC GG^CGCC
2050 GGCGCC
NcoI 2420 CCATGG C^CATGG
2717 CCATGG
NspI 60 GCATGT [GA]CATG^[CT]
761 ACATGT
PflMI 1927 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGTGTGTGG
PstI 70 CTGCAG CTGCA^G
1785 CTGCAG
SfcI 70 CTGCAG C^T[GA][CT]AG
1785 CTGCAG
SfoI 1036 GGCGCC GGC^GCC
2050 GGCGCC
SmaI 1862 CCCGGG CCC^GGG
2005 CCCGGG
StuI 1373 AGGCCT AGG^CCT
1778 AGGCCT
TaqI 923 TCGA T^CGA
1739 TCGA
TthHB8I 923 TCGA T^CGA
1739 TCGA
Van91I 1927 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2129 CCAGTGTGTGG
XcmI 992 CCATTGGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2385 CCACAGCGAGGATGG
XmaCI 1862 CCCGGG C^CCGGG
2005 CCCGGG
XmaI 1862 CCCGGG C^CCGGG
2005 CCCGGG
XmnI 936 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2289 GAAAGCCTTC
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BalI
BamHI BanI BanII BbvCI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaJI BsaWI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI Tru9I TseI
Tsp45I TspRI XhoII