(Circular) (Six-base) MAPSORT of: pFN21ASDA0685.seq Check: 2162 from: 1 to: 7510 pFN21ASDA0685 7510 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4001 4001 Size: 7510 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2568 5320 278 Size: 53 83 186 936 1032 2752 2468 Fragments arranged by size: 2752 2468 1032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4853 1937 Size: 2916 4594 Acc65I G'GTAC_C Cuts at: 4831 4831 Size: 7510 AclI AA'CG_TT Cuts at: 1563 5637 6010 1563 Size: 4074 373 3063 Fragments arranged by size: 4074 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2798 2978 3909 4303 5573 6957 7225 1632 Size: 1166 180 931 394 1270 1384 268 1917 Fragments arranged by size: 1917 1384 1270 1166 931 394 268 180 AfeI AGC'GCT Cuts at: 2505 2745 2505 Size: 240 7270 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7313 AflIII A'CryG_T Cuts at: 1203 2784 4347 6409 7386 1203 Size: 1581 1563 2062 977 1327 Fragments arranged by size: 2062 1581 1563 1327 977 AgeI A'CCGG_T Cuts at: 6341 6341 Size: 7510 AhdI GACnn_n'nnGTC Cuts at: 6239 6239 Size: 7510 AleI CACnn'nnGTG Cuts at: 1166 3347 1166 Size: 2181 5329 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2667 2723 1184 Size: 1483 56 5971 Fragments arranged by size: 5971 1483 56 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 2036 Size: 32 7478 AlwNI CAG_nnn'CTG Cuts at: 2152 2776 2902 2977 3493 5253 6825 2152 Size: 624 126 75 516 1760 1572 2837 Fragments arranged by size: 2837 1760 1572 624 516 126 75 ApaI G_GGCC'C Cuts at: 3506 4245 4713 3506 Size: 739 468 6303 Fragments arranged by size: 6303 739 468 ApaLI G'TGCA_C Cuts at: 5567 6723 5567 Size: 1156 6354 ApoI r'AATT_y Cuts at: 1493 4819 5045 5197 7306 1493 Size: 3326 226 152 2109 1697 Fragments arranged by size: 3326 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6064 160 Size: 872 5032 1606 Fragments arranged by size: 5032 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7510 AvaI C'yCGr_G Cuts at: 1870 1943 2623 3885 4028 4610 4835 1870 Size: 73 680 1262 143 582 225 4545 Fragments arranged by size: 4545 1262 680 582 225 143 73 AvrII C'CTAG_G Cuts at: 4705 4705 Size: 7510 BamHI G'GATC_C Cuts at: 2212 3257 3387 4840 5207 2212 Size: 1045 130 1453 367 4515 Fragments arranged by size: 4515 1453 1045 367 130 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 3059 4013 4073 4169 Size: 325 217 627 708 564 954 60 96 Cuts at: 4169 4831 6286 618 Size: 662 1455 1842 Fragments arranged by size: 1842 1455 954 708 662 627 564 325 217 96 60 BanII G_rGCy'C Cuts at: 1391 2556 3036 3117 3506 3543 3916 4245 4406 Size: 1165 480 81 389 37 373 329 161 Cuts at: 4406 4713 4829 1391 Size: 307 116 4072 Fragments arranged by size: 4072 1165 480 389 373 329 307 161 116 81 37 BbeI G_GCGC'C Cuts at: 3063 4077 3063 Size: 1014 6496 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6539 BbvCI CC'TCA_GC Cuts at: 2500 4174 4284 2500 Size: 1674 110 5726 Fragments arranged by size: 5726 1674 110 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5701 5735 1903 Size: 34 3764 34 3678 Fragments arranged by size: 3764 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5402 6612 5402 Size: 1210 6300 BclI T'GATC_A Cuts at: 1548 1548 Size: 7510 BfrBI ATG'CAT Cuts at: 3204 3204 Size: 7510 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2551 4022 4745 Size: 107 122 71 631 605 879 1471 723 Cuts at: 4745 6121 136 Size: 1376 1525 Fragments arranged by size: 1525 1471 1376 879 723 631 605 122 107 71 BglII A'GATC_T Cuts at: 7505 7505 Size: 7510 BlpI GC'TnA_GC Cuts at: 2330 4927 2330 Size: 2597 4913 Bme1580I G_kGCm'C Cuts at: 1790 3506 4018 4245 4608 4713 5571 6727 1790 Size: 1716 512 227 363 105 858 1156 2573 Fragments arranged by size: 2573 1716 1156 858 512 363 227 105 BmgBI CAC'GTC Cuts at: 1318 2052 3443 4144 1318 Size: 734 1391 701 4684 Fragments arranged by size: 4684 1391 734 701 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5241 6199 442 Size: 416 53 83 399 24 3824 958 1753 Fragments arranged by size: 3824 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7510 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3952 4078 3952 Size: 126 7384 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 2768 3047 3326 3413 3515 4164 6170 Size: 1502 27 279 279 87 102 649 2006 Cuts at: 6170 1239 Size: 2579 Fragments arranged by size: 2579 2006 1502 649 279 279 102 87 27 Bpu10I CC'TnA_GC Cuts at: 2500 4174 4284 2500 Size: 1674 110 5726 Fragments arranged by size: 5726 1674 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5635 6500 6798 5635 Size: 865 298 6347 Fragments arranged by size: 6347 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2685 2790 6173 882 Size: 617 1186 105 3383 2219 Fragments arranged by size: 3383 2219 1186 617 105 BsaAI yAC'GTr Cuts at: 493 1204 4807 5301 493 Size: 711 3603 494 2702 Fragments arranged by size: 3603 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7504 7504 Size: 7510 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2565 3060 4074 5317 Size: 53 83 186 936 1032 495 1014 1243 Cuts at: 5317 5699 6334 275 Size: 382 635 1451 Fragments arranged by size: 1451 1243 1032 1014 936 635 495 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2799 3297 4258 5943 6341 6615 6762 7173 2799 Size: 498 961 1685 398 274 147 411 3136 Fragments arranged by size: 3136 1685 961 498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4162 4192 4376 4406 1178 Size: 30 165 30 2759 30 184 30 4282 Fragments arranged by size: 4282 2759 184 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2201 2246 2726 3044 3251 3596 3707 4199 Size: 1015 45 480 318 207 345 111 492 Cuts at: 4199 4384 4523 1186 Size: 185 139 4173 Fragments arranged by size: 4173 1015 492 480 345 318 207 185 139 111 45 BseYI C'CCAG_C Cuts at: 2683 3098 3315 3564 3782 4194 4591 4669 4735 Size: 415 217 249 218 412 397 78 66 Cuts at: 4735 6713 7209 2683 Size: 1978 496 2984 Fragments arranged by size: 2984 1978 496 415 412 397 249 218 217 78 66 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2188 2555 3068 3401 3506 3530 4270 Size: 573 452 367 513 333 105 24 740 Cuts at: 4270 4668 1163 Size: 398 4005 Fragments arranged by size: 4005 740 573 513 452 398 367 333 105 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2305 3324 4471 4518 4960 5721 Size: 275 186 304 1019 1147 47 442 761 Cuts at: 5721 6749 1540 Size: 1028 2301 Fragments arranged by size: 2301 1147 1028 1019 761 442 304 275 186 47 BsiHKAI G_wGCw'C Cuts at: 3117 5571 5656 6727 3117 Size: 2454 85 1071 3900 Fragments arranged by size: 3900 2454 1071 85 BsmI GAATG_Cn' Cuts at: 3049 4277 5021 5114 3049 Size: 1228 744 93 5445 Fragments arranged by size: 5445 1228 744 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3433 3578 3590 3433 Size: 145 12 7353 Fragments arranged by size: 7353 145 12 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2556 3036 3117 3506 3543 3916 4018 Size: 399 766 480 81 389 37 373 102 Cuts at: 4018 4245 4406 4608 4713 4829 5571 5656 6727 Size: 227 161 202 105 116 742 85 1071 Cuts at: 6727 1391 Size: 2174 Fragments arranged by size: 2174 1071 766 742 480 399 389 373 227 202 161 116 105 102 85 81 37 BspEI T'CCGG_A Cuts at: 7173 7173 Size: 7510 BspHI T'CATG_A Cuts at: 5399 7129 5399 Size: 1730 5780 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2523 4001 4865 844 Size: 1679 1478 864 3489 Fragments arranged by size: 3489 1679 1478 864 BsrBI CCG'CTC Cuts at: 1394 1823 2734 3499 5397 1394 Size: 429 911 765 1898 3507 Fragments arranged by size: 3507 1898 911 765 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2415 6005 6179 65 Size: 1183 1167 3590 174 1396 Fragments arranged by size: 3590 1396 1183 1167 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3120 3319 4021 4542 6154 6341 1700 Size: 110 1310 199 702 521 1612 187 2869 Fragments arranged by size: 2869 1612 1310 702 521 199 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7510 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7510 BssSI C'ACGA_G Cuts at: 5570 6582 5570 Size: 1012 6498 BstAPI GCAn_nnn'nTGC Cuts at: 794 4439 794 Size: 3645 3865 BstBI TT'CG_AA Cuts at: 2007 6314 2007 Size: 4307 3203 BstEII G'GTnAC_C Cuts at: 1184 3349 1184 Size: 2165 5345 BstXI CCAn_nnnn'nTGG Cuts at: 3481 3667 4341 3481 Size: 186 674 6650 Fragments arranged by size: 6650 674 186 BstYI r'GATC_y Cuts at: 1972 2212 3257 3387 3642 4840 5207 5592 5609 Size: 240 1045 130 255 1198 367 385 17 Cuts at: 5609 7061 7147 7159 7505 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1198 1045 385 367 346 255 240 130 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3556 4443 4596 4692 4740 513 Size: 552 2491 887 153 96 48 3283 Fragments arranged by size: 3283 2491 887 552 153 96 48 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6771 Fragments arranged by size: 6771 683 56 BtsI GCAGTG_nn' Cuts at: 2183 3077 3817 4095 5026 5820 5840 2183 Size: 894 740 278 931 794 20 3853 Fragments arranged by size: 3853 931 894 794 740 278 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7510 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7475 DraI TTT'AAA Cuts at: 4814 4814 Size: 7510 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6517 809 Size: 507 5201 1802 Fragments arranged by size: 5201 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3321 3559 3894 Size: 54 1425 50 275 18 1491 238 335 Cuts at: 3894 4468 4497 4623 4689 4746 4957 5846 7475 Size: 574 29 126 66 57 211 889 1629 Cuts at: 7475 8 Size: 43 Fragments arranged by size: 1629 1491 1425 889 574 335 275 238 211 126 66 57 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3321 4468 4957 1537 Size: 275 1509 1147 489 4090 Fragments arranged by size: 4090 1509 1147 489 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2580 4684 5440 1353 Size: 1227 2104 756 3423 Fragments arranged by size: 3423 2104 1227 756 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2205 6097 6471 6617 387 Size: 1064 138 616 3892 374 146 1280 Fragments arranged by size: 3892 1280 1064 616 374 146 138 EcoICRI GAG'CTC Cuts at: 3115 3115 Size: 7510 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2741 2768 2798 2978 3047 3326 3413 Size: 393 1109 27 30 180 69 279 87 Cuts at: 3413 3515 3909 4164 4303 5573 6170 6957 7225 Size: 102 394 255 139 1270 597 787 268 Cuts at: 7225 1239 Size: 1524 Fragments arranged by size: 1524 1270 1109 787 597 394 393 279 268 255 180 139 102 87 69 30 27 EcoNI CCTnn'n_nnAGG Cuts at: 4008 4008 Size: 7510 EcoO109I rG'GnC_Cy Cuts at: 3502 3503 4241 4304 4366 4463 4477 4709 3502 Size: 1 738 63 62 97 14 232 6303 Fragments arranged by size: 6303 738 232 97 63 62 14 1 EcoRI G'AATT_C Cuts at: 4819 5197 4819 Size: 378 7132 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2056 2260 3997 2056 Size: 204 1737 5569 Fragments arranged by size: 5569 1737 204 FspI TGC'GCA Cuts at: 6016 6016 Size: 7510 HaeII r_GCGC'y Cuts at: 1678 2507 2747 3063 4077 6657 1678 Size: 829 240 316 1014 2580 2531 Fragments arranged by size: 2580 2531 1014 829 316 240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2630 2662 4376 4408 4447 Size: 32 135 32 1058 32 1714 32 39 Cuts at: 4447 4479 6151 6183 6225 6257 1373 Size: 32 1672 32 42 32 2626 Fragments arranged by size: 2626 1714 1672 1058 135 42 39 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2062 4854 5100 669 Size: 988 281 124 2792 246 3079 Fragments arranged by size: 3079 2792 988 281 246 124 HindIII A'AGCT_T Cuts at: 748 748 Size: 7510 HpaI GTT'AAC Cuts at: 5100 5100 Size: 7510 KasI G'GCGC_C Cuts at: 3059 4073 3059 Size: 1014 6496 KpnI G_GTAC'C Cuts at: 4835 4835 Size: 7510 MfeI C'AATT_G Cuts at: 5109 5109 Size: 7510 MluI A'CGCG_T Cuts at: 7386 7386 Size: 7510 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4240 6624 6808 1305 Size: 2935 2384 184 2007 Fragments arranged by size: 2935 2384 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3896 4499 4625 4748 10 Size: 54 1425 343 2064 603 126 123 2772 Fragments arranged by size: 2772 2064 1425 603 343 126 123 54 MslI CAynn'nnrTG Cuts at: 518 1166 3020 3347 3996 5468 5827 5986 518 Size: 648 1854 327 649 1472 359 159 2042 Fragments arranged by size: 2042 1854 1472 649 648 359 327 159 MspA1I CmG'CkG Cuts at: 1606 2535 2707 3255 3428 3564 4166 4486 4926 Size: 929 172 548 173 136 602 320 440 Cuts at: 4926 5603 6751 6996 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 929 677 602 548 440 320 245 173 172 136 NaeI GCC'GGC Cuts at: 3122 3321 4544 3122 Size: 199 1223 6088 Fragments arranged by size: 6088 1223 199 NarI GG'CG_CC Cuts at: 3060 4074 3060 Size: 1014 6496 NcoI C'CATG_G Cuts at: 513 1065 4443 4740 513 Size: 552 3378 297 3283 Fragments arranged by size: 3378 3283 552 297 NdeI CA'TA_TG Cuts at: 387 387 Size: 7510 NgoMIV G'CCGG_C Cuts at: 3120 3319 4542 3120 Size: 199 1223 6088 Fragments arranged by size: 6088 1223 199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7510 NotI GC'GGCC_GC Cuts at: 4957 4957 Size: 7510 NsiI A_TGCA'T Cuts at: 3206 3206 Size: 7510 NspI r_CATG'y Cuts at: 1132 1229 2087 2788 4868 6413 1132 Size: 97 858 701 2080 1545 2229 Fragments arranged by size: 2229 2080 1545 858 701 97 PasI CC'CwG_GG Cuts at: 1348 3037 4753 1348 Size: 1689 1716 4105 Fragments arranged by size: 4105 1716 1689 PciI A'CATG_T Cuts at: 2784 6409 2784 Size: 3625 3885 PflMI CCAn_nnn'nTGG Cuts at: 1108 2028 3956 4158 1108 Size: 920 1928 202 4460 Fragments arranged by size: 4460 1928 920 202 PfoI T'CCnGG_A Cuts at: 3390 3390 Size: 7510 PmeI GTTT'AAAC Cuts at: 4814 4814 Size: 7510 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5579 5611 7092 7124 5579 Size: 32 1481 32 5965 Fragments arranged by size: 5965 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4463 4463 Size: 7510 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7510 PsiI TTA'TAA Cuts at: 5080 5080 Size: 7510 PspOMI G'GGCC_C Cuts at: 3502 4241 4709 3502 Size: 739 468 6303 Fragments arranged by size: 6303 739 468 PstI C_TGCA'G Cuts at: 830 2097 3812 4862 830 Size: 1267 1715 1050 3478 Fragments arranged by size: 3478 1715 1267 1050 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7510 PvuII CAG'CTG Cuts at: 2535 2707 4166 2535 Size: 172 1459 5879 Fragments arranged by size: 5879 1459 172 SacI G_AGCT'C Cuts at: 3117 3117 Size: 7510 SalI G'TCGA_C Cuts at: 1936 4852 1936 Size: 2916 4594 SanDI GG'GwC_CC Cuts at: 4463 4463 Size: 7510 SbfI CC_TGCA'GG Cuts at: 4862 4862 Size: 7510 ScaI AGT'ACT Cuts at: 5758 7470 5758 Size: 1712 5798 SfcI C'TryA_G Cuts at: 826 1045 2093 3808 4858 5993 6674 6865 826 Size: 219 1048 1715 1050 1135 681 191 1471 Fragments arranged by size: 1715 1471 1135 1050 1048 681 219 191 SfoI GGC'GCC Cuts at: 3061 4075 3061 Size: 1014 6496 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7510 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7510 SmaI CCC'GGG Cuts at: 1872 3887 4030 4837 1872 Size: 2015 143 807 4545 Fragments arranged by size: 4545 2015 807 143 SmlI C'TyrA_G Cuts at: 820 1017 1943 5614 6515 6777 820 Size: 197 926 3671 901 262 1553 Fragments arranged by size: 3671 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4807 5301 493 Size: 4314 494 2702 Fragments arranged by size: 4314 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7510 SphI G_CATG'C Cuts at: 1132 4868 1132 Size: 3736 3774 SspI AAT'ATT Cuts at: 5 52 5434 5 Size: 47 5382 2081 Fragments arranged by size: 5382 2081 47 StuI AGG'CCT Cuts at: 1517 3398 3803 1517 Size: 1881 405 5224 Fragments arranged by size: 5224 1881 405 StyI C'CwwG_G Cuts at: 513 1065 4300 4443 4705 4740 4949 513 Size: 552 3235 143 262 35 209 3074 Fragments arranged by size: 3235 3074 552 262 209 143 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3112 5537 5554 5707 5892 6337 325 Size: 1255 1532 2425 17 153 185 445 1498 Fragments arranged by size: 2425 1532 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3162 5756 7468 Size: 274 80 33 51 1443 1185 2594 1712 Cuts at: 7468 96 Size: 138 Fragments arranged by size: 2594 1712 1443 1185 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2150 2182 2197 2229 4162 4194 4336 4368 7470 Size: 32 15 32 1933 32 142 32 3102 Cuts at: 7470 7502 2150 Size: 32 2158 Fragments arranged by size: 3102 2158 1933 142 32 32 32 32 32 15 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7510 XbaI T'CTAG_A Cuts at: 4846 4846 Size: 7510 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 4415 3022 Size: 1393 6117 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7510 XmaI C'CCGG_G Cuts at: 1870 3885 4028 4835 1870 Size: 2015 143 807 4545 Fragments arranged by size: 4545 2015 807 143 XmnI GAAnn'nnTTC Cuts at: 2963 4316 5639 2963 Size: 1353 1323 4834 Fragments arranged by size: 4834 1353 1323 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2566 5318 276 Size: 53 83 186 936 1032 2752 2468 Fragments arranged by size: 2752 2468 1032 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI