Restriction map for
pFN21ASDA0685
(Linear) (Six-base) MAPSORT of: KIAA0685.seq Check: 5215 from: 1 to: 2781
KIAA0685.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1978 2781
Size: 1978 803
AatII G_ACGT'C
Cuts at: 0 545 2781
Size: 545 2236
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 955 1886 2280 2781
Size: 775 180 931 394 501
Fragments arranged by size:
931 775 501 394 180
AfeI AGC'GCT
Cuts at: 0 482 722 2781
Size: 482 240 2059
Fragments arranged by size:
2059 482 240
AflIII A'CryG_T
Cuts at: 0 761 2324 2781
Size: 761 1563 457
Fragments arranged by size:
1563 761 457
AleI CACnn'nnGTG
Cuts at: 0 1324 2781
Size: 1324 1457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 644 700 2781
Size: 644 56 2081
Fragments arranged by size:
2081 644 56
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 2781
Size: 13 32 2736
Fragments arranged by size:
2736 32 13
AlwNI CAG_nnn'CTG
Cuts at: 0 129 753 879 954 1470 2781
Size: 129 624 126 75 516 1311
Fragments arranged by size:
1311 624 516 129 126 75
ApaI G_GGCC'C
Cuts at: 0 1483 2222 2690 2781
Size: 1483 739 468 91
Fragments arranged by size:
1483 739 468 91
AvaI C'yCGr_G
Cuts at: 0 600 1862 2005 2587 2781
Size: 600 1262 143 582 194
Fragments arranged by size:
1262 600 582 194 143
AvrII C'CTAG_G
Cuts at: 0 2682 2781
Size: 2682 99
BamHI G'GATC_C
Cuts at: 0 189 1234 1364 2781
Size: 189 1045 130 1417
Fragments arranged by size:
1417 1045 189 130
BanI G'GyrC_C
Cuts at: 0 472 1036 1990 2050 2146 2781
Size: 472 564 954 60 96 635
Fragments arranged by size:
954 635 564 472 96 60
BanII G_rGCy'C
Cuts at: 0 533 1013 1094 1483 1520 1893 2222 2383
Size: 533 480 81 389 37 373 329 161
Cuts at: 2383 2690 2781
Size: 307 91
Fragments arranged by size:
533 480 389 373 329 307 161 91
81 37
BbeI G_GCGC'C
Cuts at: 0 1040 2054 2781
Size: 1040 1014 727
Fragments arranged by size:
1040 1014 727
BbvCI CC'TCA_GC
Cuts at: 0 477 2151 2261 2781
Size: 477 1674 110 520
Fragments arranged by size:
1674 520 477 110
BfrBI ATG'CAT
Cuts at: 0 1181 2781
Size: 1181 1600
BglI GCCn_nnn'nGGC
Cuts at: 0 528 1999 2722 2781
Size: 528 1471 723 59
Fragments arranged by size:
1471 723 528 59
BlpI GC'TnA_GC
Cuts at: 0 307 2781
Size: 307 2474
Bme1580I G_kGCm'C
Cuts at: 0 1483 1995 2222 2585 2690 2781
Size: 1483 512 227 363 105 91
Fragments arranged by size:
1483 512 363 227 105 91
BmgBI CAC'GTC
Cuts at: 0 29 1420 2121 2781
Size: 29 1391 701 660
Fragments arranged by size:
1391 701 660 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1929 2055 2781
Size: 1929 126 726
Fragments arranged by size:
1929 726 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 745 1024 1303 1390 1492 2141 2781
Size: 718 27 279 279 87 102 649 640
Fragments arranged by size:
718 649 640 279 279 102 87 27
Bpu10I CC'TnA_GC
Cuts at: 0 477 2151 2261 2781
Size: 477 1674 110 520
Fragments arranged by size:
1674 520 477 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 662 767 2781
Size: 662 105 2014
Fragments arranged by size:
2014 662 105
BsaHI Gr'CG_yC
Cuts at: 0 542 1037 2051 2781
Size: 542 495 1014 730
Fragments arranged by size:
1014 730 542 495
BsaWI w'CCGG_w
Cuts at: 0 776 1274 2235 2781
Size: 776 498 961 546
Fragments arranged by size:
961 776 546 498
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2139 2169 2353 2383 2781
Size: 2139 30 184 30 398
Fragments arranged by size:
2139 398 184 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 223 703 1021 1228 1573 1684 2176
Size: 178 45 480 318 207 345 111 492
Cuts at: 2176 2361 2500 2781
Size: 185 139 281
Fragments arranged by size:
492 480 345 318 281 207 185 178
139 111 45
BseYI C'CCAG_C
Cuts at: 0 660 1075 1292 1541 1759 2171 2568 2646
Size: 660 415 217 249 218 412 397 78
Cuts at: 2646 2712 2781
Size: 66 69
Fragments arranged by size:
660 415 412 397 249 218 217 78
69 66
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 165 532 1045 1378 1483 1507 2247 2645
Size: 165 367 513 333 105 24 740 398
Cuts at: 2645 2781
Size: 136
Fragments arranged by size:
740 513 398 367 333 165 136 105
24
BsiEI CG_ry'CG
Cuts at: 0 282 1301 2448 2495 2781
Size: 282 1019 1147 47 286
Fragments arranged by size:
1147 1019 286 282 47
BsiHKAI G_wGCw'C
Cuts at: 0 1094 2781
Size: 1094 1687
BsmI GAATG_Cn'
Cuts at: 0 1026 2254 2781
Size: 1026 1228 527
Fragments arranged by size:
1228 1026 527
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1410 1555 1567 2781
Size: 1410 145 12 1214
Fragments arranged by size:
1410 1214 145 12
Bsp1286I G_dGCh'C
Cuts at: 0 533 1013 1094 1483 1520 1893 1995 2222
Size: 533 480 81 389 37 373 102 227
Cuts at: 2222 2383 2585 2690 2781
Size: 161 202 105 91
Fragments arranged by size:
533 480 389 373 227 202 161 105
102 91 81 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 500 1978 2781
Size: 500 1478 803
Fragments arranged by size:
1478 803 500
BsrBI CCG'CTC
Cuts at: 0 711 1476 2781
Size: 711 765 1305
Fragments arranged by size:
1305 765 711
BsrDI GCAATG_nn'
Cuts at: 0 392 2781
Size: 392 2389
BsrFI r'CCGG_y
Cuts at: 0 1097 1296 1998 2519 2781
Size: 1097 199 702 521 262
Fragments arranged by size:
1097 702 521 262 199
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2416 2781
Size: 2416 365
BstEII G'GTnAC_C
Cuts at: 0 1326 2781
Size: 1326 1455
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1458 1644 2318 2781
Size: 1458 186 674 463
Fragments arranged by size:
1458 674 463 186
BstYI r'GATC_y
Cuts at: 0 189 1234 1364 1619 2781
Size: 189 1045 130 255 1162
Fragments arranged by size:
1162 1045 255 189 130
BtgI C'CryG_G
Cuts at: 0 1533 2420 2573 2669 2717 2781
Size: 1533 887 153 96 48 64
Fragments arranged by size:
1533 887 153 96 64 48
BtsI GCAGTG_nn'
Cuts at: 0 160 1054 1794 2072 2781
Size: 160 894 740 278 709
Fragments arranged by size:
894 740 709 278 160
EaeI y'GGCC_r
Cuts at: 0 1298 1536 1871 2445 2474 2600 2666 2723
Size: 1298 238 335 574 29 126 66 57
Cuts at: 2723 2781
Size: 58
Fragments arranged by size:
1298 574 335 238 126 66 58 57
29
EagI C'GGCC_G
Cuts at: 0 1298 2445 2781
Size: 1298 1147 336
Fragments arranged by size:
1298 1147 336
EarI CTCTTCn'nnn_
Cuts at: 0 557 2661 2781
Size: 557 2104 120
Fragments arranged by size:
2104 557 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 182 2781
Size: 182 2599
EcoICRI GAG'CTC
Cuts at: 0 1092 2781
Size: 1092 1689
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 718 745 775 955 1024 1303 1390 1492
Size: 718 27 30 180 69 279 87 102
Cuts at: 1492 1886 2141 2280 2781
Size: 394 255 139 501
Fragments arranged by size:
718 501 394 279 255 180 139 102
87 69 30 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1985 2781
Size: 1985 796
EcoO109I rG'GnC_Cy
Cuts at: 0 1479 1480 2218 2281 2343 2440 2454 2686
Size: 1479 1 738 63 62 97 14 232
Cuts at: 2686 2781
Size: 95
Fragments arranged by size:
1479 738 232 97 95 63 62 14
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 33 237 1974 2781
Size: 33 204 1737 807
Fragments arranged by size:
1737 807 204 33
HaeII r_GCGC'y
Cuts at: 0 484 724 1040 2054 2781
Size: 484 240 316 1014 727
Fragments arranged by size:
1014 727 484 316 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 607 639 2353 2385 2424 2456 2781
Size: 607 32 1714 32 39 32 325
Fragments arranged by size:
1714 607 325 39 32 32 32
HincII GTy'rAC
Cuts at: 0 39 2781
Size: 39 2742
KasI G'GCGC_C
Cuts at: 0 1036 2050 2781
Size: 1036 1014 731
Fragments arranged by size:
1036 1014 731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2217 2781
Size: 2217 564
MscI TGG'CCA
Cuts at: 0 1873 2476 2602 2725 2781
Size: 1873 603 126 123 56
Fragments arranged by size:
1873 603 126 123 56
MslI CAynn'nnrTG
Cuts at: 0 997 1324 1973 2781
Size: 997 327 649 808
Fragments arranged by size:
997 808 649 327
MspA1I CmG'CkG
Cuts at: 0 512 684 1232 1405 1541 2143 2463 2781
Size: 512 172 548 173 136 602 320 318
Fragments arranged by size:
602 548 512 320 318 173 172 136
NaeI GCC'GGC
Cuts at: 0 1099 1298 2521 2781
Size: 1099 199 1223 260
Fragments arranged by size:
1223 1099 260 199
NarI GG'CG_CC
Cuts at: 0 1037 2051 2781
Size: 1037 1014 730
Fragments arranged by size:
1037 1014 730
NcoI C'CATG_G
Cuts at: 0 2420 2717 2781
Size: 2420 297 64
Fragments arranged by size:
2420 297 64
NgoMIV G'CCGG_C
Cuts at: 0 1097 1296 2519 2781
Size: 1097 199 1223 262
Fragments arranged by size:
1223 1097 262 199
NsiI A_TGCA'T
Cuts at: 0 1183 2781
Size: 1183 1598
NspI r_CATG'y
Cuts at: 0 64 765 2781
Size: 64 701 2016
Fragments arranged by size:
2016 701 64
PasI CC'CwG_GG
Cuts at: 0 1014 2730 2781
Size: 1014 1716 51
Fragments arranged by size:
1716 1014 51
PciI A'CATG_T
Cuts at: 0 761 2781
Size: 761 2020
PflMI CCAn_nnn'nTGG
Cuts at: 0 1933 2135 2781
Size: 1933 202 646
Fragments arranged by size:
1933 646 202
PfoI T'CCnGG_A
Cuts at: 0 1367 2781
Size: 1367 1414
PpuMI rG'GwC_Cy
Cuts at: 0 2440 2781
Size: 2440 341
PspOMI G'GGCC_C
Cuts at: 0 1479 2218 2686 2781
Size: 1479 739 468 95
Fragments arranged by size:
1479 739 468 95
PstI C_TGCA'G
Cuts at: 0 74 1789 2781
Size: 74 1715 992
Fragments arranged by size:
1715 992 74
PvuII CAG'CTG
Cuts at: 0 512 684 2143 2781
Size: 512 172 1459 638
Fragments arranged by size:
1459 638 512 172
SacI G_AGCT'C
Cuts at: 0 1094 2781
Size: 1094 1687
SanDI GG'GwC_CC
Cuts at: 0 2440 2781
Size: 2440 341
SfcI C'TryA_G
Cuts at: 0 70 1785 2781
Size: 70 1715 996
Fragments arranged by size:
1715 996 70
SfoI GGC'GCC
Cuts at: 0 1038 2052 2781
Size: 1038 1014 729
Fragments arranged by size:
1038 1014 729
SmaI CCC'GGG
Cuts at: 0 1864 2007 2781
Size: 1864 143 774
Fragments arranged by size:
1864 774 143
StuI AGG'CCT
Cuts at: 0 1375 1780 2781
Size: 1375 405 1001
Fragments arranged by size:
1375 1001 405
StyI C'CwwG_G
Cuts at: 0 2277 2420 2682 2717 2781
Size: 2277 143 262 35 64
Fragments arranged by size:
2277 262 143 64 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1089 2781
Size: 1089 1692
TatI w'GTAC_w
Cuts at: 0 1139 2781
Size: 1139 1642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 127 159 174 206 2139 2171 2313 2345
Size: 127 32 15 32 1933 32 142 32
Cuts at: 2345 2781
Size: 436
Fragments arranged by size:
1933 436 142 127 32 32 32 32
15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 999 2392 2781
Size: 999 1393 389
Fragments arranged by size:
1393 999 389
XmaI C'CCGG_G
Cuts at: 0 1862 2005 2781
Size: 1862 143 776
Fragments arranged by size:
1862 776 143
XmnI GAAnn'nnTTC
Cuts at: 0 940 2293 2781
Size: 940 1353 488
Fragments arranged by size:
1353 940 488
ZraI GAC'GTC
Cuts at: 0 543 2781
Size: 543 2238
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BfrBI BglI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BstAPI BstEII BstXI BstYI BtgI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PciI
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SanDI
SfcI SfoI SmaI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI ApaLI ApoI
AscI AseI AsiSI BaeI BbsI BcgI BciVI BclI
BglII BmrI BmtI BpuEI BsaAI BsaBI BsiWI BspEI
BspHI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI