(Circular) (Six-base) MAPSORT of: pFN21ASDA0680.seq Check: 7142 from: 1 to: 6631 pFN21ASDA0680 6631 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3832 3832 Size: 6631 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4441 278 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3898 3974 1937 Size: 1961 76 4594 Fragments arranged by size: 4594 1961 76 Acc65I G'GTAC_C Cuts at: 3952 3952 Size: 6631 AclI AA'CG_TT Cuts at: 1563 4758 5131 1563 Size: 3195 373 3063 Fragments arranged by size: 3195 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2397 3171 3276 3549 3624 4694 6078 6346 Size: 765 774 105 273 75 1070 1384 268 Cuts at: 6346 1632 Size: 1917 Fragments arranged by size: 1917 1384 1070 774 765 273 268 105 75 AflII C'TTAA_G Cuts at: 820 1017 2264 3193 820 Size: 197 1247 929 4258 Fragments arranged by size: 4258 1247 929 197 AflIII A'CryG_T Cuts at: 1203 5530 6507 1203 Size: 4327 977 1327 Fragments arranged by size: 4327 1327 977 AgeI A'CCGG_T Cuts at: 5462 5462 Size: 6631 AhdI GACnn_n'nnGTC Cuts at: 5360 5360 Size: 6631 AleI CACnn'nnGTG Cuts at: 1166 2580 1166 Size: 1414 5217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6631 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2931 2963 2931 Size: 32 6599 AlwNI CAG_nnn'CTG Cuts at: 2480 3188 3265 4374 5946 2480 Size: 708 77 1109 1572 3165 Fragments arranged by size: 3165 1572 1109 708 77 ApaLI G'TGCA_C Cuts at: 4688 5844 4688 Size: 1156 5475 ApoI r'AATT_y Cuts at: 1493 2183 2311 2978 3860 3940 4166 4318 6427 Size: 690 128 667 882 80 226 152 2109 Cuts at: 6427 1493 Size: 1697 Fragments arranged by size: 2109 1697 882 690 667 226 152 128 80 AseI AT'TA_AT Cuts at: 160 1032 5185 160 Size: 872 4153 1606 Fragments arranged by size: 4153 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 AvaI C'yCGr_G Cuts at: 1870 1943 2352 2673 3956 1870 Size: 73 409 321 1283 4545 Fragments arranged by size: 4545 1283 409 321 73 BamHI G'GATC_C Cuts at: 3738 3961 4328 3738 Size: 223 367 6041 Fragments arranged by size: 6041 367 223 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3952 5407 618 Size: 325 217 627 2165 1455 1842 Fragments arranged by size: 2165 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3950 1391 Size: 2559 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2177 3254 3399 928 Size: 971 278 1077 145 4160 Fragments arranged by size: 4160 1077 971 278 145 BbvCI CC'TCA_GC Cuts at: 2958 3258 2958 Size: 300 6331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2712 2746 4822 4856 1903 Size: 34 775 34 2076 34 3678 Fragments arranged by size: 3678 2076 775 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3445 4523 5733 3445 Size: 1078 1210 4343 Fragments arranged by size: 4343 1210 1078 BclI T'GATC_A Cuts at: 1548 2278 1548 Size: 730 5901 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5242 136 Size: 107 122 71 631 605 3570 1525 Fragments arranged by size: 3570 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6626 6626 Size: 6631 BlpI GC'TnA_GC Cuts at: 3584 3698 4048 3584 Size: 114 350 6167 Fragments arranged by size: 6167 350 114 Bme1580I G_kGCm'C Cuts at: 1790 2325 4692 5848 1790 Size: 535 2367 1156 2573 Fragments arranged by size: 2573 2367 1156 535 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6631 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4362 5320 442 Size: 416 53 83 399 24 2945 958 1753 Fragments arranged by size: 2945 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6631 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3471 3471 Size: 6631 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2438 2973 2997 3063 5291 1239 Size: 1199 535 24 66 2228 2579 Fragments arranged by size: 2579 2228 1199 535 66 24 Bpu10I CC'TnA_GC Cuts at: 2505 2958 3258 2505 Size: 453 300 5878 Fragments arranged by size: 5878 453 300 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3068 4756 5621 5919 3068 Size: 1688 865 298 3780 Fragments arranged by size: 3780 1688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2906 3700 5294 882 Size: 617 1407 794 1594 2219 Fragments arranged by size: 2219 1594 1407 794 617 BsaAI yAC'GTr Cuts at: 493 1204 3928 4422 493 Size: 711 2724 494 2702 Fragments arranged by size: 2724 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2333 6625 2333 Size: 4292 2339 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4438 4820 5455 275 Size: 53 83 186 936 2905 382 635 1451 Fragments arranged by size: 2905 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5064 5462 5736 5883 6294 5064 Size: 398 274 147 411 5401 Fragments arranged by size: 5401 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2602 2632 3760 3790 1178 Size: 30 165 30 1199 30 1128 30 4019 Fragments arranged by size: 4019 1199 1128 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 2490 2680 2975 3015 3251 1186 Size: 1069 235 190 295 40 236 4566 Fragments arranged by size: 4566 1069 295 236 235 190 40 BseYI C'CCAG_C Cuts at: 2564 3241 5834 6330 2564 Size: 677 2593 496 2865 Fragments arranged by size: 2865 2593 677 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6058 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4081 4842 5870 1540 Size: 275 186 2080 761 1028 2301 Fragments arranged by size: 2301 2080 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4692 4777 5848 4692 Size: 85 1071 5475 Fragments arranged by size: 5475 1071 85 BsmI GAATG_Cn' Cuts at: 4142 4235 4142 Size: 93 6538 BsmBI CGTCTCn'nnnn_ Cuts at: 3121 3121 Size: 6631 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2325 3950 4692 4777 5848 1391 Size: 399 535 1625 742 85 1071 2174 Fragments arranged by size: 2174 1625 1071 742 535 399 85 BspEI T'CCGG_A Cuts at: 6294 6294 Size: 6631 BspHI T'CATG_A Cuts at: 3886 4520 6250 3886 Size: 634 1730 4267 Fragments arranged by size: 4267 1730 634 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2075 3117 3832 3986 844 Size: 1231 1042 715 154 3489 Fragments arranged by size: 3489 1231 1042 715 154 BsrBI CCG'CTC Cuts at: 1394 1823 4518 1394 Size: 429 2695 3507 Fragments arranged by size: 3507 2695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5126 5300 65 Size: 1183 3878 174 1396 Fragments arranged by size: 3878 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2470 5275 5462 1700 Size: 110 660 2805 187 2869 Fragments arranged by size: 2869 2805 660 187 110 BsrGI T'GTAC_A Cuts at: 96 3372 96 Size: 3276 3355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6631 BssSI C'ACGA_G Cuts at: 4691 5703 4691 Size: 1012 5619 BstAPI GCAn_nnn'nTGC Cuts at: 794 2480 794 Size: 1686 4945 BstBI TT'CG_AA Cuts at: 2007 5435 2007 Size: 3428 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6631 BstYI r'GATC_y Cuts at: 1972 2513 3738 3961 4328 4713 4730 6182 6268 Size: 541 1225 223 367 385 17 1452 86 Cuts at: 6268 6280 6626 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1225 541 385 367 346 223 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2988 513 Size: 552 957 966 4156 Fragments arranged by size: 4156 966 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5892 Fragments arranged by size: 5892 683 56 BtsI GCAGTG_nn' Cuts at: 2559 3418 4147 4941 4961 2559 Size: 859 729 794 20 4229 Fragments arranged by size: 4229 859 794 729 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6631 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6596 DraI TTT'AAA Cuts at: 3865 3935 3865 Size: 70 6561 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3113 5638 809 Size: 507 1797 2525 1802 Fragments arranged by size: 2525 1802 1797 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4078 4967 6596 Size: 54 1425 50 275 18 2248 889 1629 Cuts at: 6596 8 Size: 43 Fragments arranged by size: 2248 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4078 1537 Size: 275 2266 4090 Fragments arranged by size: 4090 2266 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3147 3528 3642 3885 4561 1353 Size: 1794 381 114 243 676 3423 Fragments arranged by size: 3423 1794 676 381 243 114 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5218 5592 5738 387 Size: 1064 138 3629 374 146 1280 Fragments arranged by size: 3629 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2397 2438 2973 2997 3063 3171 3276 Size: 393 765 41 535 24 66 108 105 Cuts at: 3276 3549 3624 4694 5291 6078 6346 1239 Size: 273 75 1070 597 787 268 1524 Fragments arranged by size: 1524 1070 787 765 597 535 393 273 268 108 105 75 66 41 24 EcoNI CCTnn'n_nnAGG Cuts at: 2550 3811 2550 Size: 1261 5370 EcoO109I rG'GnC_Cy Cuts at: 2632 2632 Size: 6631 EcoRI G'AATT_C Cuts at: 3940 4318 3940 Size: 378 6253 EcoRV GAT'ATC Cuts at: 2220 2220 Size: 6631 FspI TGC'GCA Cuts at: 5137 5137 Size: 6631 HaeII r_GCGC'y Cuts at: 1678 5778 1678 Size: 4100 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3241 3273 3338 3370 5272 Size: 32 135 32 1669 32 65 32 1902 Cuts at: 5272 5304 5346 5378 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1902 1669 135 65 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2038 2302 3202 3819 3899 3975 Size: 988 281 100 264 900 617 80 76 Cuts at: 3975 4221 669 Size: 246 3079 Fragments arranged by size: 3079 988 900 617 281 264 246 100 80 76 HindIII A'AGCT_T Cuts at: 748 2052 2766 748 Size: 1304 714 4613 Fragments arranged by size: 4613 1304 714 HpaI GTT'AAC Cuts at: 2302 3202 3819 4221 2302 Size: 900 617 402 4712 Fragments arranged by size: 4712 900 617 402 KpnI G_GTAC'C Cuts at: 3956 3956 Size: 6631 MfeI C'AATT_G Cuts at: 4230 4230 Size: 6631 MluI A'CGCG_T Cuts at: 6507 6507 Size: 6631 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2913 3079 3574 5745 5929 1305 Size: 1608 166 495 2171 184 2007 Fragments arranged by size: 2171 2007 1608 495 184 166 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4809 Fragments arranged by size: 4809 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2580 3875 4589 4948 5107 518 Size: 648 1414 1295 714 359 159 2042 Fragments arranged by size: 2042 1414 1295 714 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4047 4724 5872 6117 1606 Size: 2441 677 1148 245 2120 Fragments arranged by size: 2441 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2988 513 Size: 552 957 966 4156 Fragments arranged by size: 4156 966 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6631 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6631 NotI GC'GGCC_GC Cuts at: 4078 4078 Size: 6631 NspI r_CATG'y Cuts at: 1132 1229 3989 5534 1132 Size: 97 2760 1545 2229 Fragments arranged by size: 2760 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3807 1348 Size: 2459 4172 PciI A'CATG_T Cuts at: 5530 5530 Size: 6631 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6631 PmeI GTTT'AAAC Cuts at: 3935 3935 Size: 6631 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4700 4732 6213 6245 4700 Size: 32 1481 32 5086 Fragments arranged by size: 5086 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3132 1613 Size: 1519 5112 PsiI TTA'TAA Cuts at: 4201 4201 Size: 6631 PstI C_TGCA'G Cuts at: 830 2911 3829 3983 830 Size: 2081 918 154 3478 Fragments arranged by size: 3478 2081 918 154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6631 SalI G'TCGA_C Cuts at: 1936 3897 3973 1936 Size: 1961 76 4594 Fragments arranged by size: 4594 1961 76 SapI GCTCTTCn'nnn_ Cuts at: 3147 3147 Size: 6631 SbfI CC_TGCA'GG Cuts at: 3983 3983 Size: 6631 ScaI AGT'ACT Cuts at: 4879 6591 4879 Size: 1712 4919 SfcI C'TryA_G Cuts at: 826 1045 2058 2907 3825 3979 5114 5795 5986 Size: 219 1013 849 918 154 1135 681 191 Cuts at: 5986 826 Size: 1471 Fragments arranged by size: 1471 1135 1013 918 849 681 219 191 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6631 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6631 SmaI CCC'GGG Cuts at: 1872 3958 1872 Size: 2086 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2264 3047 3193 4735 5636 5898 Size: 197 926 321 783 146 1542 901 262 Cuts at: 5898 820 Size: 1553 Fragments arranged by size: 1553 1542 926 901 783 321 262 197 146 SnaBI TAC'GTA Cuts at: 493 3928 4422 493 Size: 3435 494 2702 Fragments arranged by size: 3435 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6631 SphI G_CATG'C Cuts at: 1132 3989 1132 Size: 2857 3774 SspI AAT'ATT Cuts at: 5 52 4555 5 Size: 47 4503 2081 Fragments arranged by size: 4503 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6631 StyI C'CwwG_G Cuts at: 513 1065 2022 2150 2613 2814 2988 4070 513 Size: 552 957 128 463 201 174 1082 3074 Fragments arranged by size: 3074 1082 957 552 463 201 174 128 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2662 4658 4675 4828 5013 5458 325 Size: 1255 1082 1996 17 153 185 445 1498 Fragments arranged by size: 1996 1498 1255 1082 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3372 4877 6589 Size: 274 80 33 51 1443 1395 1505 1712 Cuts at: 6589 96 Size: 138 Fragments arranged by size: 1712 1505 1443 1395 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3760 3792 6591 6623 3760 Size: 32 2799 32 3768 Fragments arranged by size: 3768 2799 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6631 XbaI T'CTAG_A Cuts at: 3967 3967 Size: 6631 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3238 3238 Size: 6631 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6631 XmaI C'CCGG_G Cuts at: 1870 3956 1870 Size: 2086 4545 XmnI GAAnn'nnTTC Cuts at: 4760 4760 Size: 6631 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4439 276 Size: 53 83 186 936 2905 2468 Fragments arranged by size: 2905 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI