(Circular) (Six-base) MAPSORT of: pFN21ASDA0666.seq Check: 4468 from: 1 to: 7963 pFN21ASDA0666 7963 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3532 3532 Size: 7963 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5773 278 Size: 53 83 186 936 4237 2468 Fragments arranged by size: 4237 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5306 1937 Size: 3369 4594 Acc65I G'GTAC_C Cuts at: 5284 5284 Size: 7963 AclI AA'CG_TT Cuts at: 1563 6090 6463 1563 Size: 4527 373 3063 Fragments arranged by size: 4527 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 3836 4816 6026 7410 7678 1632 Size: 488 1716 980 1210 1384 268 1917 Fragments arranged by size: 1917 1716 1384 1210 980 488 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7766 AflIII A'CryG_T Cuts at: 1203 4200 6862 7839 1203 Size: 2997 2662 977 1327 Fragments arranged by size: 2997 2662 1327 977 AgeI A'CCGG_T Cuts at: 6794 6794 Size: 7963 AhdI GACnn_n'nnGTC Cuts at: 6692 6692 Size: 7963 AleI CACnn'nnGTG Cuts at: 1166 4610 1166 Size: 3444 4519 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7963 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2174 2206 3995 4027 2174 Size: 32 1789 32 6110 Fragments arranged by size: 6110 1789 32 32 AlwNI CAG_nnn'CTG Cuts at: 2656 3418 4462 5706 7278 2656 Size: 762 1044 1244 1572 3341 Fragments arranged by size: 3341 1572 1244 1044 762 ApaI G_GGCC'C Cuts at: 3639 3639 Size: 7963 ApaLI G'TGCA_C Cuts at: 6020 7176 6020 Size: 1156 6807 ApoI r'AATT_y Cuts at: 1493 2333 2942 2958 2995 3056 3936 4220 5272 Size: 840 609 16 37 61 880 284 1052 Cuts at: 5272 5498 5650 7759 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1052 880 840 609 284 226 152 61 37 16 AseI AT'TA_AT Cuts at: 160 1032 2637 2801 2877 4323 6517 160 Size: 872 1605 164 76 1446 2194 1606 Fragments arranged by size: 2194 1606 1605 1446 872 164 76 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7963 AvaI C'yCGr_G Cuts at: 1870 1943 2723 3878 5288 1870 Size: 73 780 1155 1410 4545 Fragments arranged by size: 4545 1410 1155 780 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3912 3945 3912 Size: 33 7930 BamHI G'GATC_C Cuts at: 5293 5660 5293 Size: 367 7596 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2705 4445 5284 6739 618 Size: 325 217 627 918 1740 839 1455 1842 Fragments arranged by size: 1842 1740 1455 918 839 627 325 217 BanII G_rGCy'C Cuts at: 1391 2611 3576 3639 4102 5035 5282 1391 Size: 1220 965 63 463 933 247 4072 Fragments arranged by size: 4072 1220 965 933 463 247 63 BbeI G_GCGC'C Cuts at: 2709 2709 Size: 7963 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3922 3957 4857 928 Size: 971 2023 35 900 4034 Fragments arranged by size: 4034 2023 971 900 35 BbvCI CC'TCA_GC Cuts at: 3834 3834 Size: 7963 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6154 6188 1903 Size: 34 4217 34 3678 Fragments arranged by size: 4217 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2513 5855 7065 2513 Size: 3342 1210 3411 Fragments arranged by size: 3411 3342 1210 BclI T'GATC_A Cuts at: 1548 2086 2344 4951 1548 Size: 538 258 2607 4560 Fragments arranged by size: 4560 2607 538 258 BfrBI ATG'CAT Cuts at: 4517 4517 Size: 7963 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6574 136 Size: 107 122 71 631 605 4902 1525 Fragments arranged by size: 4902 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3943 4186 7958 3943 Size: 243 3772 3948 Fragments arranged by size: 3948 3772 243 BlpI GC'TnA_GC Cuts at: 2888 3575 5046 5380 2888 Size: 687 1471 334 5471 Fragments arranged by size: 5471 1471 687 334 Bme1580I G_kGCm'C Cuts at: 1790 3639 4450 6024 7180 1790 Size: 1849 811 1574 1156 2573 Fragments arranged by size: 2573 1849 1574 1156 811 BmgBI CAC'GTC Cuts at: 1318 2614 1318 Size: 1296 6667 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2835 4623 5694 Size: 416 53 83 399 24 1418 1788 1071 Cuts at: 5694 6652 442 Size: 958 1753 Fragments arranged by size: 1788 1753 1418 1071 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7963 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3642 3717 4484 6623 1239 Size: 2403 75 767 2139 2579 Fragments arranged by size: 2579 2403 2139 767 75 Bpu10I CC'TnA_GC Cuts at: 3834 5146 3834 Size: 1312 6651 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2583 2837 3438 4436 4653 4738 6088 6953 7251 Size: 254 601 998 217 85 1350 865 298 Cuts at: 7251 2583 Size: 3295 Fragments arranged by size: 3295 1350 998 865 601 298 254 217 85 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2528 5227 6626 882 Size: 617 1029 2699 1399 2219 Fragments arranged by size: 2699 2219 1399 1029 617 BsaAI yAC'GTr Cuts at: 493 1204 2103 5260 5754 493 Size: 711 899 3157 494 2702 Fragments arranged by size: 3157 2702 899 711 494 BsaBI GATnn'nnATC Cuts at: 3260 7957 3260 Size: 4697 3266 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2706 5770 6152 6787 Size: 53 83 186 936 1173 3064 382 635 Cuts at: 6787 275 Size: 1451 Fragments arranged by size: 3064 1451 1173 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6396 6794 7068 7215 7626 6396 Size: 398 274 147 411 6733 Fragments arranged by size: 6733 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3275 3305 3995 4025 1178 Size: 30 165 30 1872 30 690 30 5116 Fragments arranged by size: 5116 1872 690 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2189 3217 3735 3738 3753 1186 Size: 1003 1028 518 3 15 5396 Fragments arranged by size: 5396 1028 1003 518 15 3 BseYI C'CCAG_C Cuts at: 7166 7662 7166 Size: 496 7467 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2741 2919 3158 1163 Size: 573 1005 178 239 5968 Fragments arranged by size: 5968 1005 573 239 178 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5413 6174 7202 1540 Size: 275 186 3412 761 1028 2301 Fragments arranged by size: 3412 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2663 2889 3399 3576 3628 3799 4102 5035 6024 Size: 226 510 177 52 171 303 933 989 Cuts at: 6024 6109 7180 2663 Size: 85 1071 3446 Fragments arranged by size: 3446 1071 989 933 510 303 226 177 171 85 52 BsiWI C'GTAC_G Cuts at: 3328 3328 Size: 7963 BsmI GAATG_Cn' Cuts at: 3828 4519 5474 5567 3828 Size: 691 955 93 6224 Fragments arranged by size: 6224 955 691 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2611 2663 2889 3399 3576 3628 3639 Size: 399 821 52 226 510 177 52 11 Cuts at: 3639 3799 4102 4450 5035 5282 6024 6109 7180 Size: 160 303 348 585 247 742 85 1071 Cuts at: 7180 1391 Size: 2174 Fragments arranged by size: 2174 1071 821 742 585 510 399 348 303 247 226 177 160 85 52 52 11 BspEI T'CCGG_A Cuts at: 7626 7626 Size: 7963 BspHI T'CATG_A Cuts at: 5852 7582 5852 Size: 1730 6233 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3532 5318 844 Size: 2688 1786 3489 Fragments arranged by size: 3489 2688 1786 BsrBI CCG'CTC Cuts at: 1394 1823 5850 1394 Size: 429 4027 3507 Fragments arranged by size: 4027 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2153 6458 6632 65 Size: 1183 905 4305 174 1396 Fragments arranged by size: 4305 1396 1183 905 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6607 6794 1700 Size: 110 4797 187 2869 Fragments arranged by size: 4797 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7963 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7963 BssSI C'ACGA_G Cuts at: 6023 7035 6023 Size: 1012 6951 BstAPI GCAn_nnn'nTGC Cuts at: 794 2752 4342 794 Size: 1958 1590 4415 Fragments arranged by size: 4415 1958 1590 BstBI TT'CG_AA Cuts at: 2007 6767 2007 Size: 4760 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7963 BstXI CCAn_nnnn'nTGG Cuts at: 3301 3301 Size: 7963 BstYI r'GATC_y Cuts at: 1972 3659 3943 4186 5293 5660 6045 6062 7514 Size: 1687 284 243 1107 367 385 17 1452 Cuts at: 7514 7600 7612 7958 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1687 1452 1107 385 367 346 284 243 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3769 3769 Size: 7963 BtgI C'CryG_G Cuts at: 513 1065 2022 2142 2358 2523 513 Size: 552 957 120 216 165 5953 Fragments arranged by size: 5953 957 552 216 165 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7224 Fragments arranged by size: 7224 683 56 BtsI GCAGTG_nn' Cuts at: 2889 3178 3368 4896 5479 6273 6293 2889 Size: 289 190 1528 583 794 20 4559 Fragments arranged by size: 4559 1528 794 583 289 190 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7963 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3187 3222 529 Size: 35 2623 35 5270 Fragments arranged by size: 5270 2623 35 35 DraI TTT'AAA Cuts at: 2433 5267 2433 Size: 2834 5129 DraIII CAC_nnn'GTG Cuts at: 3143 3143 Size: 7963 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2542 6970 809 Size: 507 1226 4428 1802 Fragments arranged by size: 4428 1802 1226 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 4284 5410 Size: 54 1425 50 275 18 855 1599 1126 Cuts at: 5410 6299 7928 8 Size: 889 1629 43 Fragments arranged by size: 1629 1599 1425 1126 889 855 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5410 1537 Size: 275 3598 4090 Fragments arranged by size: 4090 3598 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3122 3459 4422 4902 5893 1353 Size: 1769 337 963 480 991 3423 Fragments arranged by size: 3423 1769 991 963 480 337 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3563 6550 6924 7070 387 Size: 1064 138 1974 2987 374 146 1280 Fragments arranged by size: 2987 1974 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3574 4100 5033 3574 Size: 526 933 6504 Fragments arranged by size: 6504 933 526 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 3642 3717 3836 4484 4816 6026 Size: 393 488 1522 75 119 648 332 1210 Cuts at: 6026 6623 7410 7678 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1522 1210 787 648 597 488 393 332 268 119 75 EcoNI CCTnn'n_nnAGG Cuts at: 3693 3753 3768 4253 3693 Size: 60 15 485 7403 Fragments arranged by size: 7403 485 60 15 EcoO109I rG'GnC_Cy Cuts at: 2784 3283 3635 3636 3758 2784 Size: 499 352 1 122 6989 Fragments arranged by size: 6989 499 352 122 1 EcoRI G'AATT_C Cuts at: 2333 2958 5272 5650 2333 Size: 625 2314 378 4646 Fragments arranged by size: 4646 2314 625 378 EcoRV GAT'ATC Cuts at: 4533 4533 Size: 7963 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3518 3518 Size: 7963 FspI TGC'GCA Cuts at: 6469 6469 Size: 7963 HaeII r_GCGC'y Cuts at: 1678 2709 7110 1678 Size: 1031 4401 2531 Fragments arranged by size: 4401 2531 1031 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2480 2512 3275 3307 4075 Size: 32 135 32 908 32 763 32 768 Cuts at: 4075 4107 4547 4579 6604 6636 6678 6710 1373 Size: 32 440 32 2025 32 42 32 2626 Fragments arranged by size: 2626 2025 908 768 763 440 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5307 5553 669 Size: 988 281 3369 246 3079 Fragments arranged by size: 3369 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3147 748 Size: 2399 5564 HpaI GTT'AAC Cuts at: 5553 5553 Size: 7963 KasI G'GCGC_C Cuts at: 2705 2705 Size: 7963 KpnI G_GTAC'C Cuts at: 5288 5288 Size: 7963 MfeI C'AATT_G Cuts at: 5562 5562 Size: 7963 MluI A'CGCG_T Cuts at: 7839 7839 Size: 7963 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4073 4185 7077 7261 1305 Size: 2768 112 2892 184 2007 Fragments arranged by size: 2892 2768 2007 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 4286 10 Size: 54 1425 343 2454 3687 Fragments arranged by size: 3687 2454 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4610 5921 6280 6439 518 Size: 648 3444 1311 359 159 2042 Fragments arranged by size: 3444 2042 1311 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2258 5379 6056 7204 7449 1606 Size: 652 3121 677 1148 245 2120 Fragments arranged by size: 3121 2120 1148 677 652 245 NarI GG'CG_CC Cuts at: 2706 2706 Size: 7963 NcoI C'CATG_G Cuts at: 513 1065 2022 2142 2358 2523 513 Size: 552 957 120 216 165 5953 Fragments arranged by size: 5953 957 552 216 165 120 NdeI CA'TA_TG Cuts at: 387 4519 387 Size: 4132 3831 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7963 NotI GC'GGCC_GC Cuts at: 5410 5410 Size: 7963 NsiI A_TGCA'T Cuts at: 4519 4519 Size: 7963 NspI r_CATG'y Cuts at: 1132 1229 4204 5321 6866 1132 Size: 97 2975 1117 1545 2229 Fragments arranged by size: 2975 2229 1545 1117 97 PacI TTA_AT'TAA Cuts at: 2805 2805 Size: 7963 PasI CC'CwG_GG Cuts at: 1348 3755 1348 Size: 2407 5556 PciI A'CATG_T Cuts at: 4200 6862 4200 Size: 2662 5301 PflMI CCAn_nnn'nTGG Cuts at: 1108 3791 1108 Size: 2683 5280 PmeI GTTT'AAAC Cuts at: 5267 5267 Size: 7963 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2202 2234 3995 4027 6032 6064 7545 7577 2202 Size: 32 1761 32 2005 32 1481 32 2588 Fragments arranged by size: 2588 2005 1761 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2784 3283 2784 Size: 499 7464 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7963 PsiI TTA'TAA Cuts at: 5533 5533 Size: 7963 PspOMI G'GGCC_C Cuts at: 3635 3635 Size: 7963 PstI C_TGCA'G Cuts at: 830 2748 5315 830 Size: 1918 2567 3478 Fragments arranged by size: 3478 2567 1918 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7963 PvuII CAG'CTG Cuts at: 2258 2258 Size: 7963 SacI G_AGCT'C Cuts at: 3576 4102 5035 3576 Size: 526 933 6504 Fragments arranged by size: 6504 933 526 SalI G'TCGA_C Cuts at: 1936 5305 1936 Size: 3369 4594 SbfI CC_TGCA'GG Cuts at: 5315 5315 Size: 7963 ScaI AGT'ACT Cuts at: 3224 6211 7923 3224 Size: 2987 1712 3264 Fragments arranged by size: 3264 2987 1712 SexAI A'CCwGG_T Cuts at: 3694 3694 Size: 7963 SfcI C'TryA_G Cuts at: 826 1045 2281 2421 2744 5311 6446 7127 7318 Size: 219 1236 140 323 2567 1135 681 191 Cuts at: 7318 826 Size: 1471 Fragments arranged by size: 2567 1471 1236 1135 681 323 219 191 140 SfoI GGC'GCC Cuts at: 2707 2707 Size: 7963 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7963 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7963 SmaI CCC'GGG Cuts at: 1872 2725 5290 1872 Size: 853 2565 4545 Fragments arranged by size: 4545 2565 853 SmlI C'TyrA_G Cuts at: 820 1017 1943 2598 2852 3453 4451 4668 4717 Size: 197 926 655 254 601 998 217 49 Cuts at: 4717 6067 6968 7230 820 Size: 1350 901 262 1553 Fragments arranged by size: 1553 1350 998 926 901 655 601 262 254 217 197 49 SnaBI TAC'GTA Cuts at: 493 5260 5754 493 Size: 4767 494 2702 Fragments arranged by size: 4767 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7963 SphI G_CATG'C Cuts at: 1132 5321 1132 Size: 4189 3774 SspI AAT'ATT Cuts at: 5 52 3316 5887 5 Size: 47 3264 2571 2081 Fragments arranged by size: 3264 2571 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7963 StyI C'CwwG_G Cuts at: 513 1065 2022 2142 2358 2523 2893 4194 4287 Size: 552 957 120 216 165 370 1301 93 Cuts at: 4287 5402 513 Size: 1115 3074 Fragments arranged by size: 3074 1301 1115 957 552 370 216 165 120 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2106 3923 5990 6007 6160 6345 6790 Size: 1255 526 1817 2067 17 153 185 445 Cuts at: 6790 325 Size: 1498 Fragments arranged by size: 2067 1817 1498 1255 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 4353 6209 Size: 274 80 33 51 1443 1245 1131 1856 Cuts at: 6209 7921 96 Size: 1712 138 Fragments arranged by size: 1856 1712 1443 1245 1131 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7923 7955 7923 Size: 32 7931 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7963 XbaI T'CTAG_A Cuts at: 3940 5299 3940 Size: 1359 6604 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2167 4201 2167 Size: 2034 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7963 XmaI C'CCGG_G Cuts at: 1870 2723 5288 1870 Size: 853 2565 4545 Fragments arranged by size: 4545 2565 853 XmnI GAAnn'nnTTC Cuts at: 3078 3857 4410 4854 6092 3078 Size: 779 553 444 1238 4949 Fragments arranged by size: 4949 1238 779 553 444 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5771 276 Size: 53 83 186 936 4237 2468 Fragments arranged by size: 4237 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BplI BsmBI BstZ17I FseI FspAI NaeI NgoMIV NruI PfoI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI