(Circular) (Six-base) MAPSORT of: pFN21ASDA0665.seq Check: 94 from: 1 to: 6997 pFN21ASDA0665 6997 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2725 3924 2725 Size: 1199 5798 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2502 4807 278 Size: 53 83 186 936 966 2305 2468 Fragments arranged by size: 2468 2305 966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2496 2721 4340 1937 Size: 559 225 1619 4594 Fragments arranged by size: 4594 1619 559 225 Acc65I G'GTAC_C Cuts at: 3324 4318 3324 Size: 994 6003 AclI AA'CG_TT Cuts at: 1563 5124 5497 1563 Size: 3561 373 3063 Fragments arranged by size: 3561 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3057 3566 3737 4052 4073 5060 6444 6712 Size: 1425 509 171 315 21 987 1384 268 Cuts at: 6712 1632 Size: 1917 Fragments arranged by size: 1917 1425 1384 987 509 315 268 171 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6800 AflIII A'CryG_T Cuts at: 1203 5896 6873 1203 Size: 4693 977 1327 Fragments arranged by size: 4693 1327 977 AgeI A'CCGG_T Cuts at: 3470 5828 3470 Size: 2358 4639 AhdI GACnn_n'nnGTC Cuts at: 2173 3594 5726 2173 Size: 1421 2132 3444 Fragments arranged by size: 3444 2132 1421 AleI CACnn'nnGTG Cuts at: 1166 3014 1166 Size: 1848 5149 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3032 3989 1184 Size: 1848 957 4192 Fragments arranged by size: 4192 1848 957 AlwNI CAG_nnn'CTG Cuts at: 3559 3673 3757 3883 4740 6312 3559 Size: 114 84 126 857 1572 4244 Fragments arranged by size: 4244 1572 857 126 114 84 ApaI G_GGCC'C Cuts at: 2073 2129 2345 3346 3395 2073 Size: 56 216 1001 49 5675 Fragments arranged by size: 5675 1001 216 56 49 ApaLI G'TGCA_C Cuts at: 3017 3515 5054 6210 3017 Size: 498 1539 1156 3804 Fragments arranged by size: 3804 1539 1156 498 ApoI r'AATT_y Cuts at: 1493 4306 4532 4684 6793 1493 Size: 2813 226 152 2109 1697 Fragments arranged by size: 2813 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5551 160 Size: 872 4519 1606 Fragments arranged by size: 4519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 2050 2179 2201 2248 2276 2338 2408 Size: 73 107 129 22 47 28 62 70 Cuts at: 2408 2555 3377 3996 4170 4322 1870 Size: 147 822 619 174 152 4545 Fragments arranged by size: 4545 822 619 174 152 147 129 107 73 70 62 47 28 22 BamHI G'GATC_C Cuts at: 2761 4327 4694 2761 Size: 1566 367 5064 Fragments arranged by size: 5064 1566 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3113 3324 3846 4106 4318 Size: 325 217 627 1326 211 522 260 212 Cuts at: 4318 5773 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1326 627 522 325 260 217 212 211 BanII G_rGCy'C Cuts at: 1391 2057 2073 2129 2330 2345 2409 2613 2775 Size: 666 16 56 201 15 64 204 162 Cuts at: 2775 2883 2960 3105 3346 3384 3395 3609 4182 Size: 108 77 145 241 38 11 214 573 Cuts at: 4182 4316 1391 Size: 134 4072 Fragments arranged by size: 4072 666 573 241 214 204 201 162 145 134 108 77 64 56 38 16 15 11 BbeI G_GCGC'C Cuts at: 4110 4110 Size: 6997 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2494 2799 3415 928 Size: 971 595 305 616 4510 Fragments arranged by size: 4510 971 616 595 305 BbvCI CC'TCA_GC Cuts at: 2582 4094 2582 Size: 1512 5485 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3095 3129 3625 3659 5188 5222 1903 Size: 34 1158 34 496 34 1529 34 3678 Fragments arranged by size: 3678 1529 1158 496 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4889 6099 4889 Size: 1210 5787 BclI T'GATC_A Cuts at: 1548 2781 1548 Size: 1233 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2039 2068 2089 Size: 107 122 71 631 605 367 29 21 Cuts at: 2089 2098 2243 5608 136 Size: 9 145 3365 1525 Fragments arranged by size: 3365 1525 631 605 367 145 122 107 71 29 21 9 BglII A'GATC_T Cuts at: 6992 6992 Size: 6997 BlpI GC'TnA_GC Cuts at: 4414 4414 Size: 6997 Bme1580I G_kGCm'C Cuts at: 1790 2073 2129 2345 3021 3075 3118 3346 3395 Size: 283 56 216 676 54 43 228 49 Cuts at: 3395 3519 5058 6214 1790 Size: 124 1539 1156 2573 Fragments arranged by size: 2573 1539 1156 676 283 228 216 124 56 54 49 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6997 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2760 3279 4728 Size: 416 53 83 399 24 1343 519 1449 Cuts at: 4728 5686 442 Size: 958 1753 Fragments arranged by size: 1753 1449 1343 958 519 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4177 4196 4177 Size: 19 6978 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2564 3289 3383 3464 3554 3758 3929 Size: 1037 288 725 94 81 90 204 171 Cuts at: 3929 3953 4031 4301 5657 1239 Size: 24 78 270 1356 2579 Fragments arranged by size: 2579 1356 1037 725 288 270 204 171 94 90 81 78 24 Bpu10I CC'TnA_GC Cuts at: 2582 3024 4094 2582 Size: 442 1070 5485 Fragments arranged by size: 5485 1070 442 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3911 5122 5987 6285 3911 Size: 1211 865 298 4623 Fragments arranged by size: 4623 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2265 2978 3227 3581 4256 5660 882 Size: 617 766 713 249 354 675 1404 2219 Fragments arranged by size: 2219 1404 766 713 675 617 354 249 BsaAI yAC'GTr Cuts at: 493 1204 4294 4788 493 Size: 711 3090 494 2702 Fragments arranged by size: 3090 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6991 6991 Size: 6997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2027 2334 2499 3090 Size: 53 83 186 936 494 307 165 591 Cuts at: 3090 3595 4107 4804 5186 5821 275 Size: 505 512 697 382 635 1451 Fragments arranged by size: 1451 936 697 635 591 512 505 494 382 307 186 165 83 53 BsaWI w'CCGG_w Cuts at: 3470 3696 5430 5828 6102 6249 6660 3470 Size: 226 1734 398 274 147 411 3807 Fragments arranged by size: 3807 1734 411 398 274 226 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2139 2169 2942 2972 3596 Size: 30 165 30 736 30 773 30 624 Cuts at: 3596 3626 4274 4304 1178 Size: 30 648 30 3871 Fragments arranged by size: 3871 773 736 648 624 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 2426 2891 3113 3197 3413 3551 3695 Size: 1231 9 465 222 84 216 138 144 Cuts at: 3695 3755 3758 3829 3914 4082 4220 1186 Size: 60 3 71 85 168 138 3963 Fragments arranged by size: 3963 1231 465 222 216 168 144 138 138 85 84 71 60 9 3 BseYI C'CCAG_C Cuts at: 3253 6200 6696 3253 Size: 2947 496 3554 Fragments arranged by size: 3554 2947 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3314 3688 1163 Size: 573 1578 374 4472 Fragments arranged by size: 4472 1578 573 374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3069 4447 5208 6236 1540 Size: 275 186 1068 1378 761 1028 2301 Fragments arranged by size: 2301 1378 1068 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2969 3021 3189 3384 3519 3609 3915 4182 5058 Size: 52 168 195 135 90 306 267 876 Cuts at: 5058 5143 6214 2969 Size: 85 1071 3752 Fragments arranged by size: 3752 1071 876 306 267 195 168 135 90 85 52 BsmI GAATG_Cn' Cuts at: 4508 4601 4508 Size: 93 6904 BsmBI CGTCTCn'nnnn_ Cuts at: 4171 4171 Size: 6997 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2073 2129 2330 2345 2409 2613 Size: 399 267 16 56 201 15 64 204 Cuts at: 2613 2775 2883 2960 2969 3021 3075 3105 3118 Size: 162 108 77 9 52 54 30 13 Cuts at: 3118 3189 3346 3384 3395 3519 3609 3915 4182 Size: 71 157 38 11 124 90 306 267 Cuts at: 4182 4316 5058 5143 6214 1391 Size: 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 267 267 204 201 162 157 134 124 108 90 85 77 71 64 56 54 52 38 30 16 15 13 11 9 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 4886 6616 4886 Size: 1730 5267 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2725 3336 3513 3660 3924 4352 844 Size: 1881 611 177 147 264 428 3489 Fragments arranged by size: 3489 1881 611 428 264 177 147 BsrBI CCG'CTC Cuts at: 1394 1823 4884 1394 Size: 429 3061 3507 Fragments arranged by size: 3507 3061 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3405 5492 5666 65 Size: 1183 2157 2087 174 1396 Fragments arranged by size: 2157 2087 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 2242 3056 3470 5641 5828 1700 Size: 110 228 204 814 414 2171 187 2869 Fragments arranged by size: 2869 2171 814 414 228 204 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6997 BssHII G'CGCG_C Cuts at: 1925 2442 1925 Size: 517 6480 BssSI C'ACGA_G Cuts at: 3839 5057 6069 3839 Size: 1218 1012 4767 Fragments arranged by size: 4767 1218 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3053 3514 794 Size: 2259 461 4277 Fragments arranged by size: 4277 2259 461 BstBI TT'CG_AA Cuts at: 2007 5801 2007 Size: 3794 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6997 BstXI CCAn_nnnn'nTGG Cuts at: 3013 3013 Size: 6997 BstYI r'GATC_y Cuts at: 1972 2761 2830 4327 4694 5079 5096 6548 6634 Size: 789 69 1497 367 385 17 1452 86 Cuts at: 6634 6646 6992 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1497 1452 789 385 367 346 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 3491 3491 Size: 6997 BtgI C'CryG_G Cuts at: 513 1065 2022 2309 2523 2613 3093 3109 3148 Size: 552 957 287 214 90 480 16 39 Cuts at: 3148 3351 513 Size: 203 4159 Fragments arranged by size: 4159 957 552 480 287 214 203 90 39 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6258 Fragments arranged by size: 6258 683 56 BtsI GCAGTG_nn' Cuts at: 3217 3268 4513 5307 5327 3217 Size: 51 1245 794 20 4887 Fragments arranged by size: 4887 1245 794 51 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6997 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6962 DraI TTT'AAA Cuts at: 4301 4301 Size: 6997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2684 3419 4270 6004 809 Size: 507 1368 735 851 1734 1802 Fragments arranged by size: 1802 1734 1368 851 735 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2839 3066 3151 Size: 54 1425 50 275 18 1009 227 85 Cuts at: 3151 4251 4444 5333 6962 8 Size: 1100 193 889 1629 43 Fragments arranged by size: 1629 1425 1100 1009 889 275 227 193 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3066 4444 1537 Size: 275 1254 1378 4090 Fragments arranged by size: 4090 1378 1254 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3579 3626 3792 4124 4927 1353 Size: 2226 47 166 332 803 3423 Fragments arranged by size: 3423 2226 803 332 166 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2275 3233 4208 5584 5958 6104 Size: 1064 138 686 958 975 1376 374 146 Cuts at: 6104 387 Size: 1280 Fragments arranged by size: 1376 1280 1064 975 958 686 374 146 138 EcoICRI GAG'CTC Cuts at: 3382 3607 4180 3382 Size: 225 573 6199 Fragments arranged by size: 6199 573 225 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2276 2564 3057 3289 3383 3464 3554 Size: 393 644 288 493 232 94 81 90 Cuts at: 3554 3566 3737 3758 3929 3953 4031 4052 4073 Size: 12 171 21 171 24 78 21 21 Cuts at: 4073 4301 5060 5657 6444 6712 1239 Size: 228 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 644 597 493 393 288 268 232 228 171 171 94 90 81 78 24 21 21 21 12 EcoNI CCTnn'n_nnAGG Cuts at: 3494 3494 Size: 6997 EcoO109I rG'GnC_Cy Cuts at: 2069 2126 2552 2559 3342 3343 3358 3367 3391 Size: 57 426 7 783 1 15 9 24 Cuts at: 3391 3892 2069 Size: 501 5174 Fragments arranged by size: 5174 783 501 426 57 24 15 9 7 1 EcoRI G'AATT_C Cuts at: 4306 4684 4306 Size: 378 6619 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2384 3439 4135 2384 Size: 1055 696 5246 Fragments arranged by size: 5246 1055 696 FseI GG_CCGG'CC Cuts at: 2246 2246 Size: 6997 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 2153 2238 2366 2543 4110 6144 1678 Size: 475 85 128 177 1567 2034 2531 Fragments arranged by size: 2531 2034 1567 475 177 128 85 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2139 2159 2171 2191 2489 Size: 32 135 32 567 20 12 20 298 Cuts at: 2489 2521 3580 3612 3758 3790 5638 5670 5712 Size: 32 1059 32 146 32 1848 32 42 Cuts at: 5712 5744 1373 Size: 32 2626 Fragments arranged by size: 2626 1848 1059 567 298 146 135 42 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2497 4341 4587 669 Size: 988 281 559 1844 246 3079 Fragments arranged by size: 3079 1844 988 559 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6997 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KasI G'GCGC_C Cuts at: 4106 4106 Size: 6997 KpnI G_GTAC'C Cuts at: 3328 4322 3328 Size: 994 6003 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2210 3314 3741 6111 6295 1305 Size: 905 1104 427 2370 184 2007 Fragments arranged by size: 2370 2007 1104 905 427 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2841 3153 4253 10 Size: 54 1425 343 1009 312 1100 2754 Fragments arranged by size: 2754 1425 1100 1009 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 3014 3147 3167 4955 5314 5473 518 Size: 648 1848 133 20 1788 359 159 2042 Fragments arranged by size: 2042 1848 1788 648 359 159 133 20 MspA1I CmG'CkG Cuts at: 1606 2210 2311 3253 3511 3544 3907 3946 4413 Size: 604 101 942 258 33 363 39 467 Cuts at: 4413 5090 6238 6483 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 942 677 604 467 363 258 245 101 39 33 NaeI GCC'GGC Cuts at: 2040 2244 3058 2040 Size: 204 814 5979 Fragments arranged by size: 5979 814 204 NarI GG'CG_CC Cuts at: 4107 4107 Size: 6997 NcoI C'CATG_G Cuts at: 513 1065 2022 3093 3109 3148 3351 513 Size: 552 957 1071 16 39 203 4159 Fragments arranged by size: 4159 1071 957 552 203 39 16 NdeI CA'TA_TG Cuts at: 387 387 Size: 6997 NgoMIV G'CCGG_C Cuts at: 2038 2242 3056 2038 Size: 204 814 5979 Fragments arranged by size: 5979 814 204 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 4444 4444 Size: 6997 NspI r_CATG'y Cuts at: 1132 1229 4355 5900 1132 Size: 97 3126 1545 2229 Fragments arranged by size: 3126 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2345 1348 Size: 997 6000 PciI A'CATG_T Cuts at: 5896 5896 Size: 6997 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6997 PfoI T'CCnGG_A Cuts at: 2393 3426 3579 4278 2393 Size: 1033 153 699 5112 Fragments arranged by size: 5112 1033 699 153 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5066 5098 6579 6611 5066 Size: 32 1481 32 5452 Fragments arranged by size: 5452 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2552 2559 3367 3892 2552 Size: 7 808 525 5657 Fragments arranged by size: 5657 808 525 7 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 4567 4567 Size: 6997 PspOMI G'GGCC_C Cuts at: 2069 2125 2341 3342 3391 2069 Size: 56 216 1001 49 5675 Fragments arranged by size: 5675 1001 216 56 49 PstI C_TGCA'G Cuts at: 830 2215 3263 3510 3657 3921 3978 4153 4227 Size: 1385 1048 247 147 264 57 175 74 Cuts at: 4227 4349 830 Size: 122 3478 Fragments arranged by size: 3478 1385 1048 264 247 175 147 122 74 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 PvuII CAG'CTG Cuts at: 3253 3511 3544 3907 3253 Size: 258 33 363 6343 Fragments arranged by size: 6343 363 258 33 SacI G_AGCT'C Cuts at: 3384 3609 4182 3384 Size: 225 573 6199 Fragments arranged by size: 6199 573 225 SacII CC_GC'GG Cuts at: 2312 2312 Size: 6997 SalI G'TCGA_C Cuts at: 1936 2495 4339 1936 Size: 559 1844 4594 Fragments arranged by size: 4594 1844 559 SanDI GG'GwC_CC Cuts at: 2559 2559 Size: 6997 SapI GCTCTTCn'nnn_ Cuts at: 3626 3792 3626 Size: 166 6831 SbfI CC_TGCA'GG Cuts at: 3978 4227 4349 3978 Size: 249 122 6626 Fragments arranged by size: 6626 249 122 ScaI AGT'ACT Cuts at: 2756 5245 6957 2756 Size: 2489 1712 2796 Fragments arranged by size: 2796 2489 1712 SfcI C'TryA_G Cuts at: 826 1045 2211 2971 3259 3506 3653 3665 3917 Size: 219 1166 760 288 247 147 12 252 Cuts at: 3917 3974 4149 4223 4345 5480 6161 6352 826 Size: 57 175 74 122 1135 681 191 1471 Fragments arranged by size: 1471 1166 1135 760 681 288 252 247 219 191 175 147 122 74 57 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2039 2098 2039 Size: 59 6938 SfoI GGC'GCC Cuts at: 4108 4108 Size: 6997 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 3470 1700 Size: 1770 5227 SmaI CCC'GGG Cuts at: 1872 2052 2181 2203 2250 2278 2340 2557 3998 Size: 180 129 22 47 28 62 217 1441 Cuts at: 3998 4324 1872 Size: 326 4545 Fragments arranged by size: 4545 1441 326 217 180 129 62 47 28 22 SmlI C'TyrA_G Cuts at: 820 1017 1943 3926 5101 6002 6264 820 Size: 197 926 1983 1175 901 262 1553 Fragments arranged by size: 1983 1553 1175 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4294 4788 493 Size: 3801 494 2702 Fragments arranged by size: 3801 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 4355 1132 Size: 3223 3774 SspI AAT'ATT Cuts at: 5 52 4921 5 Size: 47 4869 2081 Fragments arranged by size: 4869 2081 47 StuI AGG'CCT Cuts at: 1517 3139 1517 Size: 1622 5375 StyI C'CwwG_G Cuts at: 513 1065 2022 2803 3093 3109 3148 3351 4436 Size: 552 957 781 290 16 39 203 1085 Cuts at: 4436 513 Size: 3074 Fragments arranged by size: 3074 1085 957 781 552 290 203 39 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3890 5024 5041 5194 5379 5824 325 Size: 1255 2310 1134 17 153 185 445 1498 Fragments arranged by size: 2310 1498 1255 1134 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2754 5243 6955 Size: 274 80 33 51 1443 777 2489 1712 Cuts at: 6955 96 Size: 138 Fragments arranged by size: 2489 1712 1443 777 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3271 3303 3596 3628 6957 6989 3271 Size: 32 293 32 3329 32 3279 Fragments arranged by size: 3329 3279 293 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2912 1316 Size: 1596 5401 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2849 3313 3358 2849 Size: 464 45 6488 Fragments arranged by size: 6488 464 45 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6997 XmaI C'CCGG_G Cuts at: 1870 2050 2179 2201 2248 2276 2338 2555 3996 Size: 180 129 22 47 28 62 217 1441 Cuts at: 3996 4322 1870 Size: 326 4545 Fragments arranged by size: 4545 1441 326 217 180 129 62 47 28 22 XmnI GAAnn'nnTTC Cuts at: 5126 5126 Size: 6997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2500 4805 276 Size: 53 83 186 936 966 2305 2468 Fragments arranged by size: 2468 2305 966 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SrfI SwaI