Restriction map for
pFN21ASDA0659
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# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BfaI BfrI BglII
BlnI BsiWI BsmBI BspDI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI Eco47III
EcoRV FspI HincII HindII HindIII
HpaI KpnI MaeI MfeI MluI
MseI MunI NdeI NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SbfI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI SwaI TfiI Tru9I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 2280 GTCTAC GT^[AC][GT]AC
AccIII 3056 TCCGGA T^CCGGA
ApaI 2844 GGGCCC GGGCC^C
BbrPI 1819 CACGTG CAC^GTG
BbvCI 2839 GCTGAGG CCTCAGC(-5/-2)
BsaI 1364 GAGACC GGTCTC(1/5)
BsaWI 3056 TCCGGA [AT]^CCGG[AT]
BseAI 3056 TCCGGA T^CCGGA
BsmAI 1364 GAGAC GTCTC(1/5)
BsmI 3034 GAATGC GAATGC(1/-1)
Bsp1407I 2366 TGTACA T^GTACA
BspEI 3056 TCCGGA T^CCGGA
BsrGI 2366 TGTACA T^GTACA
BstAPI 282 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2428 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1344 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1683 ATGCAT ATGCA^T
FseI 2663 GGCCGGCC GGCCGG^CC
MroI 3056 TCCGGA T^CCGGA
NcoI 2336 CCATGG C^CATGG
NsiI 1683 ATGCAT ATGCA^T
PmaCI 1819 CACGTG CAC^GTG
PmlI 1819 CACGTG CAC^GTG
SapI 117 GCTCTTC GCTCTTC(1/4)
ScaI 362 AGTACT AGT^ACT
SmaI 4 CCCGGG CCC^GGG
SphI 239 GCATGC GCATG^C
SspBI 2366 TGTACA T^GTACA
XmaCI 4 CCCGGG C^CCGGG
XmaI 4 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 843 GAATTC [GA]^AATT[CT]
2734 GAATTC
ApoI 843 GAATTC [GA]^AATT[CT]
2734 GAATTC
BbeI 1064 GGCGCC GGCGC^C
2699 GGCGCC
BciVI 1187 GTATCC GTATCC(6/5)
1464 GTATCC
BclI 1181 TGATCA T^GATCA
1919 TGATCA
BsaBI 219 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1182 GATCAGTATC
BsgI 609 CTGCAC GTGCAG(16/14)
2689 CTGCAC
BssSI 142 CACGAG CACGAG(-5/-1)
972 CTCGTG
BstEII 810 GGTGACC G^GT[ACGT]ACC
1719 GGTGACC
BstPI 810 GGTGACC G^GT[ACGT]ACC
1719 GGTGACC
BstXI 1350 CCAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2030 CCAAGGTCATGG
BstYI 2870 AGATCC [GA]^GATC[CT]
3053 AGATCC
EcoO65I 810 GGTGACC G^GT[ACGT]ACC
1719 GGTGACC
EcoRI 843 GAATTC G^AATTC
2734 GAATTC
FbaI 1181 TGATCA T^GATCA
1919 TGATCA
KasI 1064 GGCGCC G^GCGCC
2699 GGCGCC
MamI 219 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1182 GATCAGTATC
MflI 2870 AGATCC [GA]^GATC[CT]
3053 AGATCC
NarI 1064 GGCGCC GG^CGCC
2699 GGCGCC
NspI 239 GCATGC [GA]CATG^[CT]
884 ACATGC
SfoI 1064 GGCGCC GGC^GCC
2699 GGCGCC
SmlI 838 CTCAAG C^T[CT][GA]AG
1474 CTCAAG
Tsp509I 844 AATT ^AATT
2735 AATT
XhoII 2870 AGATCC [GA]^GATC[CT]
3053 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AocI AspEI
AspHI AspI AvaI AvaII BalI
BanI BanII BbsI BbvI BcgI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaJI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflFI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI StuI StyI TaqI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I