(Circular) (Six-base) MAPSORT of: pFN21ASDA0659.seq Check: 3663 from: 1 to: 7855 pFN21ASDA0659 7855 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3981 3981 Size: 7855 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5665 278 Size: 53 83 186 936 4129 2468 Fragments arranged by size: 4129 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4304 5198 1937 Size: 2367 894 4594 Fragments arranged by size: 4594 2367 894 Acc65I G'GTAC_C Cuts at: 5176 5176 Size: 7855 AclI AA'CG_TT Cuts at: 1563 5982 6355 1563 Size: 4419 373 3063 Fragments arranged by size: 4419 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5918 7302 7570 1632 Size: 4286 1384 268 1917 Fragments arranged by size: 4286 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7658 AflIII A'CryG_T Cuts at: 1203 6754 7731 1203 Size: 5551 977 1327 Fragments arranged by size: 5551 1327 977 AgeI A'CCGG_T Cuts at: 6686 6686 Size: 7855 AhdI GACnn_n'nnGTC Cuts at: 2653 4381 4558 6584 2653 Size: 1728 177 2026 3924 Fragments arranged by size: 3924 2026 1728 177 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7855 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2798 3886 4556 4850 1184 Size: 1614 1088 670 294 4189 Fragments arranged by size: 4189 1614 1088 670 294 AlwNI CAG_nnn'CTG Cuts at: 3646 4588 4753 5598 7170 3646 Size: 942 165 845 1572 4331 Fragments arranged by size: 4331 1572 942 845 165 ApaI G_GGCC'C Cuts at: 4871 4871 Size: 7855 ApaLI G'TGCA_C Cuts at: 5912 7068 5912 Size: 1156 6699 ApoI r'AATT_y Cuts at: 1493 2866 4757 5164 5390 5542 7651 1493 Size: 1373 1891 407 226 152 2109 1697 Fragments arranged by size: 2109 1891 1697 1373 407 226 152 AseI AT'TA_AT Cuts at: 160 1032 6409 160 Size: 872 5377 1606 Fragments arranged by size: 5377 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7855 AvaI C'yCGr_G Cuts at: 1870 1943 2027 4505 4838 4972 5011 5180 1870 Size: 73 84 2478 333 134 39 169 4545 Fragments arranged by size: 4545 2478 333 169 134 84 73 39 BamHI G'GATC_C Cuts at: 5185 5552 5185 Size: 367 7488 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2571 3087 3285 3293 4345 Size: 325 217 627 784 516 198 8 1052 Cuts at: 4345 4722 4834 4927 5176 6631 618 Size: 377 112 93 249 1455 1842 Fragments arranged by size: 1842 1455 1052 784 627 516 377 325 249 217 198 112 93 8 BanII G_rGCy'C Cuts at: 1391 2292 3897 3999 4070 4098 4439 4529 4847 Size: 901 1605 102 71 28 341 90 318 Cuts at: 4847 4871 4920 5022 5174 1391 Size: 24 49 102 152 4072 Fragments arranged by size: 4072 1605 901 341 318 152 102 102 90 71 49 28 24 BbeI G_GCGC'C Cuts at: 3091 4726 3091 Size: 1635 6220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2482 3057 4037 928 Size: 971 583 575 980 4746 Fragments arranged by size: 4746 980 971 583 575 BbvCI CC'TCA_GC Cuts at: 4863 4863 Size: 7855 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2393 2427 2714 2748 3577 3611 6046 Size: 34 456 34 287 34 829 34 2435 Cuts at: 6046 6080 1903 Size: 34 3678 Fragments arranged by size: 3678 2435 829 456 287 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3221 3498 5747 6957 3221 Size: 277 2249 1210 4119 Fragments arranged by size: 4119 2249 1210 277 BclI T'GATC_A Cuts at: 1548 3204 3942 1548 Size: 1656 738 5461 Fragments arranged by size: 5461 1656 738 BfrBI ATG'CAT Cuts at: 3708 3708 Size: 7855 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3679 4224 4343 Size: 107 122 71 631 605 2007 545 119 Cuts at: 4343 4942 6466 136 Size: 599 1524 1525 Fragments arranged by size: 2007 1525 1524 631 605 599 545 122 119 107 71 BglII A'GATC_T Cuts at: 7850 7850 Size: 7855 BlpI GC'TnA_GC Cuts at: 2222 2258 3264 4957 5003 5272 2222 Size: 36 1006 1693 46 269 4805 Fragments arranged by size: 4805 1693 1006 269 46 36 Bme1580I G_kGCm'C Cuts at: 1790 3288 3452 4348 4839 4871 4909 4932 5916 Size: 1498 164 896 491 32 38 23 984 Cuts at: 5916 7072 1790 Size: 1156 2573 Fragments arranged by size: 2573 1498 1156 984 896 491 164 38 32 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7855 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2458 2647 4198 Size: 416 53 83 399 24 1041 189 1551 Cuts at: 4198 4629 4734 4903 5586 6544 442 Size: 431 105 169 683 958 1753 Fragments arranged by size: 1753 1551 1041 958 683 431 416 399 189 169 105 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7855 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2191 4246 4387 2191 Size: 2055 141 5659 Fragments arranged by size: 5659 2055 141 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3054 3371 4031 4175 4241 4481 4610 6515 Size: 1815 317 660 144 66 240 129 1905 Cuts at: 6515 1239 Size: 2579 Fragments arranged by size: 2579 1905 1815 660 317 240 144 129 66 Bpu10I CC'TnA_GC Cuts at: 4092 4523 4623 4863 4092 Size: 431 100 240 7084 Fragments arranged by size: 7084 431 240 100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2846 3482 5980 6845 7143 2846 Size: 636 2498 865 298 3558 Fragments arranged by size: 3558 2498 865 636 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3381 6518 882 Size: 617 1882 3137 2219 Fragments arranged by size: 3137 2219 1882 617 BsaAI yAC'GTr Cuts at: 493 1204 2311 2320 3172 3844 5152 5646 493 Size: 711 1107 9 852 672 1308 494 2702 Fragments arranged by size: 2702 1308 1107 852 711 672 494 9 BsaBI GATnn'nnATC Cuts at: 2246 3209 7849 2246 Size: 963 4640 2252 Fragments arranged by size: 4640 2252 963 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3088 4614 4723 5033 Size: 53 83 186 936 1555 1526 109 310 Cuts at: 5033 5662 6044 6679 275 Size: 629 382 635 1451 Fragments arranged by size: 1555 1526 1451 936 635 629 382 310 186 109 83 53 BsaWI w'CCGG_w Cuts at: 5079 6288 6686 6960 7107 7518 5079 Size: 1209 398 274 147 411 5416 Fragments arranged by size: 5416 1209 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2378 2408 2530 2560 1178 Size: 30 165 30 975 30 122 30 6473 Fragments arranged by size: 6473 975 165 122 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2551 2597 3746 3905 4402 4670 4960 4999 Size: 1365 46 1149 159 497 268 290 39 Cuts at: 4999 1186 Size: 4042 Fragments arranged by size: 4042 1365 1149 497 290 268 159 46 39 BseYI C'CCAG_C Cuts at: 3301 3779 4442 4880 7058 7554 3301 Size: 478 663 438 2178 496 3602 Fragments arranged by size: 3602 2178 663 496 478 438 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2617 4697 1163 Size: 573 881 2080 4321 Fragments arranged by size: 4321 2080 881 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2200 3832 4551 5305 6066 7094 Size: 275 186 199 1632 719 754 761 1028 Cuts at: 7094 1540 Size: 2301 Fragments arranged by size: 2301 1632 1028 761 754 719 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2142 2316 3972 4098 4529 5022 5916 6001 7072 Size: 174 1656 126 431 493 894 85 1071 Cuts at: 7072 2142 Size: 2925 Fragments arranged by size: 2925 1656 1071 894 493 431 174 126 85 BsmI GAATG_Cn' Cuts at: 5063 5366 5459 5063 Size: 303 93 7459 Fragments arranged by size: 7459 303 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2292 2316 3288 3452 3897 3972 Size: 399 352 150 24 972 164 445 75 Cuts at: 3972 3999 4070 4098 4348 4439 4529 4839 4847 Size: 27 71 28 250 91 90 310 8 Cuts at: 4847 4871 4909 4920 4932 5022 5174 5916 6001 Size: 24 38 11 12 90 152 742 85 Cuts at: 6001 7072 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 972 742 445 399 352 310 250 164 152 150 91 90 90 85 75 71 38 28 27 24 24 12 11 8 BspEI T'CCGG_A Cuts at: 5079 7518 5079 Size: 2439 5416 BspHI T'CATG_A Cuts at: 5744 7474 5744 Size: 1730 6125 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2559 2574 3981 5210 844 Size: 1715 15 1407 1229 3489 Fragments arranged by size: 3489 1715 1407 1229 15 BsrBI CCG'CTC Cuts at: 1394 1823 4037 4315 4410 4627 5002 5742 1394 Size: 429 2214 278 95 217 375 740 3507 Fragments arranged by size: 3507 2214 740 429 375 278 217 95 BsrDI GCAATG_nn' Cuts at: 65 1248 2730 3075 3552 6350 6524 65 Size: 1183 1482 345 477 2798 174 1396 Fragments arranged by size: 2798 1482 1396 1183 477 345 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2610 2830 4687 4952 6499 6686 1700 Size: 110 800 220 1857 265 1547 187 2869 Fragments arranged by size: 2869 1857 1547 800 265 220 187 110 BsrGI T'GTAC_A Cuts at: 96 4389 96 Size: 4293 3562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7855 BssSI C'ACGA_G Cuts at: 2165 2995 5915 6927 2165 Size: 830 2920 1012 3093 Fragments arranged by size: 3093 2920 1012 830 BstAPI GCAn_nnn'nTGC Cuts at: 794 2311 794 Size: 1517 6338 BstBI TT'CG_AA Cuts at: 2007 6659 2007 Size: 4652 3203 BstEII G'GTnAC_C Cuts at: 1184 2833 3742 1184 Size: 1649 909 5297 Fragments arranged by size: 5297 1649 909 BstXI CCAn_nnnn'nTGG Cuts at: 3380 4060 3380 Size: 680 7175 BstYI r'GATC_y Cuts at: 1972 4893 5076 5185 5552 5937 5954 7406 7492 Size: 2921 183 109 367 385 17 1452 86 Cuts at: 7492 7504 7850 1972 Size: 12 346 1977 Fragments arranged by size: 2921 1977 1452 385 367 346 183 109 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3729 4414 4746 3729 Size: 685 332 6838 Fragments arranged by size: 6838 685 332 BtgI C'CryG_G Cuts at: 513 1065 2133 2194 2200 2499 3020 3276 3289 Size: 552 1068 61 6 299 521 256 13 Cuts at: 3289 3397 4122 4359 4696 4940 5037 5101 513 Size: 108 725 237 337 244 97 64 3267 Fragments arranged by size: 3267 1068 725 552 521 337 299 256 244 237 108 97 64 61 13 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7116 Fragments arranged by size: 7116 683 56 BtsI GCAGTG_nn' Cuts at: 2071 2409 3450 3875 4612 5371 6165 6185 2071 Size: 338 1041 425 737 759 794 20 3741 Fragments arranged by size: 3741 1041 794 759 737 425 338 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7855 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2101 2136 3043 3078 3886 3921 4129 Size: 35 1537 35 907 35 808 35 208 Cuts at: 4129 4164 529 Size: 35 4220 Fragments arranged by size: 4220 1537 907 808 208 35 35 35 35 35 DraI TTT'AAA Cuts at: 5159 5159 Size: 7855 DraIII CAC_nnn'GTG Cuts at: 4456 4456 Size: 7855 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6862 809 Size: 507 5546 1802 Fragments arranged by size: 5546 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2197 2244 2328 Size: 54 1425 50 275 18 367 47 84 Cuts at: 2328 3753 3829 4353 4548 4644 5098 5302 6191 Size: 1425 76 524 195 96 454 204 889 Cuts at: 6191 7820 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 524 454 367 275 204 195 96 84 76 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2197 3829 4548 5302 1537 Size: 275 385 1632 719 754 4090 Fragments arranged by size: 4090 1632 754 719 385 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 4487 4656 4787 5785 1353 Size: 794 2340 169 131 998 3423 Fragments arranged by size: 3423 2340 998 794 169 131 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3697 4340 6442 6816 6962 387 Size: 1064 138 2108 643 2102 374 146 1280 Fragments arranged by size: 2108 2102 1280 1064 643 374 146 138 EcoICRI GAG'CTC Cuts at: 4096 4527 5020 4096 Size: 431 493 6931 Fragments arranged by size: 6931 493 431 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3054 3371 4031 4175 4241 4481 4610 Size: 393 1422 317 660 144 66 240 129 Cuts at: 4610 5918 6515 7302 7570 1239 Size: 1308 597 787 268 1524 Fragments arranged by size: 1524 1422 1308 787 660 597 393 317 268 240 144 129 66 EcoNI CCTnn'n_nnAGG Cuts at: 3371 3371 Size: 7855 EcoO109I rG'GnC_Cy Cuts at: 2704 3219 3526 4279 4867 4868 2704 Size: 515 307 753 588 1 5691 Fragments arranged by size: 5691 753 588 515 307 1 EcoRI G'AATT_C Cuts at: 2866 4757 5164 5542 2866 Size: 1891 407 378 5179 Fragments arranged by size: 5179 1891 407 378 FseI GG_CCGG'CC Cuts at: 4691 4691 Size: 7855 FspI TGC'GCA Cuts at: 6361 6361 Size: 7855 HaeII r_GCGC'y Cuts at: 1678 2053 3091 3271 4712 4726 7002 1678 Size: 375 1038 180 1441 14 2276 2531 Fragments arranged by size: 2531 2276 1441 1038 375 180 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2639 2651 2671 2683 3027 Size: 32 135 32 1067 12 20 12 344 Cuts at: 3027 3059 4367 4399 4544 4576 4955 4987 6496 Size: 32 1308 32 145 32 379 32 1509 Cuts at: 6496 6528 6570 6602 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1509 1308 1067 379 344 145 135 42 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 5199 5445 669 Size: 988 281 3261 246 3079 Fragments arranged by size: 3261 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7855 HpaI GTT'AAC Cuts at: 5445 5445 Size: 7855 KasI G'GCGC_C Cuts at: 3087 4722 3087 Size: 1635 6220 KpnI G_GTAC'C Cuts at: 5180 5180 Size: 7855 MfeI C'AATT_G Cuts at: 5454 5454 Size: 7855 MluI A'CGCG_T Cuts at: 7731 7731 Size: 7855 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2748 3515 3699 6969 7153 1305 Size: 1443 767 184 3270 184 2007 Fragments arranged by size: 3270 2007 1443 767 184 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2246 2330 3755 4355 5100 Size: 54 1425 343 414 84 1425 600 745 Cuts at: 5100 10 Size: 2765 Fragments arranged by size: 2765 1425 1425 745 600 414 343 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2336 3842 4832 5813 6172 6331 518 Size: 648 1170 1506 990 981 359 159 2042 Fragments arranged by size: 2042 1506 1170 990 981 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 2601 2758 2764 3022 3329 3399 3622 Size: 596 399 157 6 258 307 70 223 Cuts at: 3622 4698 5271 5948 7096 7341 1606 Size: 1076 573 677 1148 245 2120 Fragments arranged by size: 2120 1148 1076 677 596 573 399 307 258 245 223 157 70 6 NaeI GCC'GGC Cuts at: 2612 4689 4954 2612 Size: 2077 265 5513 Fragments arranged by size: 5513 2077 265 NarI GG'CG_CC Cuts at: 3088 4723 3088 Size: 1635 6220 NcoI C'CATG_G Cuts at: 513 1065 4359 513 Size: 552 3294 4009 Fragments arranged by size: 4009 3294 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7855 NgoMIV G'CCGG_C Cuts at: 2610 4687 4952 2610 Size: 2077 265 5513 Fragments arranged by size: 5513 2077 265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7855 NotI GC'GGCC_GC Cuts at: 5302 5302 Size: 7855 NsiI A_TGCA'T Cuts at: 3710 3710 Size: 7855 NspI r_CATG'y Cuts at: 1132 1229 2266 2911 5213 6758 1132 Size: 97 1037 645 2302 1545 2229 Fragments arranged by size: 2302 2229 1545 1037 645 97 PasI CC'CwG_GG Cuts at: 1348 3439 4600 1348 Size: 2091 1161 4603 Fragments arranged by size: 4603 2091 1161 PciI A'CATG_T Cuts at: 6754 6754 Size: 7855 PflMI CCAn_nnn'nTGG Cuts at: 1108 2253 2601 3097 3439 1108 Size: 1145 348 496 342 5524 Fragments arranged by size: 5524 1145 496 348 342 PfoI T'CCnGG_A Cuts at: 2700 3096 3645 2700 Size: 396 549 6910 Fragments arranged by size: 6910 549 396 PmeI GTTT'AAAC Cuts at: 5159 5159 Size: 7855 PmlI CAC'GTG Cuts at: 3844 3844 Size: 7855 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5924 5956 7437 7469 5924 Size: 32 1481 32 6310 Fragments arranged by size: 6310 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2704 3219 4279 2704 Size: 515 1060 6280 Fragments arranged by size: 6280 1060 515 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7855 PsiI TTA'TAA Cuts at: 5425 5425 Size: 7855 PspOMI G'GGCC_C Cuts at: 4867 4867 Size: 7855 PstI C_TGCA'G Cuts at: 830 3660 3865 4051 4171 4335 5207 830 Size: 2830 205 186 120 164 872 3478 Fragments arranged by size: 3478 2830 872 205 186 164 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7855 PvuII CAG'CTG Cuts at: 2601 3329 3622 2601 Size: 728 293 6834 Fragments arranged by size: 6834 728 293 SacI G_AGCT'C Cuts at: 4098 4529 5022 4098 Size: 431 493 6931 Fragments arranged by size: 6931 493 431 SacII CC_GC'GG Cuts at: 2203 3023 3400 4699 2203 Size: 820 377 1299 5359 Fragments arranged by size: 5359 1299 820 377 SalI G'TCGA_C Cuts at: 1936 5197 1936 Size: 3261 4594 SanDI GG'GwC_CC Cuts at: 3219 3219 Size: 7855 SapI GCTCTTCn'nnn_ Cuts at: 2147 2147 Size: 7855 SbfI CC_TGCA'GG Cuts at: 5207 5207 Size: 7855 ScaI AGT'ACT Cuts at: 2387 6103 7815 2387 Size: 3716 1712 2427 Fragments arranged by size: 3716 2427 1712 SexAI A'CCwGG_T Cuts at: 2114 2184 4923 2114 Size: 70 2739 5046 Fragments arranged by size: 5046 2739 70 SfcI C'TryA_G Cuts at: 826 1045 3656 3792 3861 4047 4167 4331 5203 Size: 219 2611 136 69 186 120 164 872 Cuts at: 5203 6338 7019 7210 826 Size: 1135 681 191 1471 Fragments arranged by size: 2611 1471 1135 872 681 219 191 186 164 136 120 69 SfoI GGC'GCC Cuts at: 3089 4724 3089 Size: 1635 6220 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7855 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7855 SmaI CCC'GGG Cuts at: 1872 2029 5182 1872 Size: 157 3153 4545 Fragments arranged by size: 4545 3153 157 SmlI C'TyrA_G Cuts at: 820 1017 1943 2861 3497 5959 6860 7122 820 Size: 197 926 918 636 2462 901 262 1553 Fragments arranged by size: 2462 1553 926 918 901 636 262 197 SnaBI TAC'GTA Cuts at: 493 5152 5646 493 Size: 4659 494 2702 Fragments arranged by size: 4659 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7855 SphI G_CATG'C Cuts at: 1132 2266 5213 1132 Size: 1134 2947 3774 Fragments arranged by size: 3774 2947 1134 SspI AAT'ATT Cuts at: 5 52 5779 5 Size: 47 5727 2081 Fragments arranged by size: 5727 2081 47 StuI AGG'CCT Cuts at: 1517 2171 3377 3929 1517 Size: 654 1206 552 5443 Fragments arranged by size: 5443 1206 654 552 StyI C'CwwG_G Cuts at: 513 1065 3231 3558 4053 4359 5294 513 Size: 552 2166 327 495 306 935 3074 Fragments arranged by size: 3074 2166 935 552 495 327 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2976 5882 5899 6052 6237 6682 325 Size: 1255 1396 2906 17 153 185 445 1498 Fragments arranged by size: 2906 1498 1396 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 3333 4389 Size: 274 80 33 51 1443 408 948 1056 Cuts at: 4389 6101 7813 96 Size: 1712 1712 138 Fragments arranged by size: 1712 1712 1443 1056 948 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7815 7847 7815 Size: 32 7823 Tth111I GACn'n_nGTC Cuts at: 1316 2075 3125 3223 3596 1316 Size: 759 1050 98 373 5575 Fragments arranged by size: 5575 1050 759 373 98 XbaI T'CTAG_A Cuts at: 5191 5191 Size: 7855 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7855 XmaI C'CCGG_G Cuts at: 1870 2027 5180 1870 Size: 157 3153 4545 Fragments arranged by size: 4545 3153 157 XmnI GAAnn'nnTTC Cuts at: 5984 5984 Size: 7855 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5663 276 Size: 53 83 186 936 4129 2468 Fragments arranged by size: 4129 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I EcoRV FalI FspAI NruI PacI PspXI PsrI RsrII SfiI SrfI SwaI XcmI