Restriction map for
pFN21ASDA0659
(Linear) (Six-base) MAPSORT of: KIAA0659.seq Check: 2909 from: 1 to: 3126
KIAA0659.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1958 3126
Size: 1958 1168
AccI GT'mk_AC
Cuts at: 0 2281 3126
Size: 2281 845
AhdI GACnn_n'nnGTC
Cuts at: 0 630 2358 2535 3126
Size: 630 1728 177 591
Fragments arranged by size:
1728 630 591 177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 775 1863 2533 2827 3126
Size: 775 1088 670 294 299
Fragments arranged by size:
1088 775 670 299 294
AlwNI CAG_nnn'CTG
Cuts at: 0 1623 2565 2730 3126
Size: 1623 942 165 396
Fragments arranged by size:
1623 942 396 165
ApaI G_GGCC'C
Cuts at: 0 2848 3126
Size: 2848 278
ApoI r'AATT_y
Cuts at: 0 843 2734 3126
Size: 843 1891 392
Fragments arranged by size:
1891 843 392
AvaI C'yCGr_G
Cuts at: 0 4 2482 2815 2949 2988 3126
Size: 4 2478 333 134 39 138
Fragments arranged by size:
2478 333 138 134 39 4
BanI G'GyrC_C
Cuts at: 0 548 1064 1262 1270 2322 2699 2811 2904
Size: 548 516 198 8 1052 377 112 93
Cuts at: 2904 3126
Size: 222
Fragments arranged by size:
1052 548 516 377 222 198 112 93
8
BanII G_rGCy'C
Cuts at: 0 269 1874 1976 2047 2075 2416 2506 2824
Size: 269 1605 102 71 28 341 90 318
Cuts at: 2824 2848 2897 2999 3126
Size: 24 49 102 127
Fragments arranged by size:
1605 341 318 269 127 102 102 90
71 49 28 24
BbeI G_GCGC'C
Cuts at: 0 1068 2703 3126
Size: 1068 1635 423
Fragments arranged by size:
1635 1068 423
BbsI GAAGACnn'nnnn_
Cuts at: 0 459 1034 2014 3126
Size: 459 575 980 1112
Fragments arranged by size:
1112 980 575 459
BbvCI CC'TCA_GC
Cuts at: 0 2840 3126
Size: 2840 286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 370 404 691 725 1554 1588 3126
Size: 370 34 287 34 829 34 1538
Fragments arranged by size:
1538 829 370 287 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1198 1475 3126
Size: 1198 277 1651
Fragments arranged by size:
1651 1198 277
BclI T'GATC_A
Cuts at: 0 1181 1919 3126
Size: 1181 738 1207
Fragments arranged by size:
1207 1181 738
BfrBI ATG'CAT
Cuts at: 0 1685 3126
Size: 1685 1441
BglI GCCn_nnn'nGGC
Cuts at: 0 1656 2201 2320 2919 3126
Size: 1656 545 119 599 207
Fragments arranged by size:
1656 599 545 207 119
BlpI GC'TnA_GC
Cuts at: 0 199 235 1241 2934 2980 3126
Size: 199 36 1006 1693 46 146
Fragments arranged by size:
1693 1006 199 146 46 36
Bme1580I G_kGCm'C
Cuts at: 0 1265 1429 2325 2816 2848 2886 2909 3126
Size: 1265 164 896 491 32 38 23 217
Fragments arranged by size:
1265 896 491 217 164 38 32 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 624 2175 2606 2711 2880 3126
Size: 435 189 1551 431 105 169 246
Fragments arranged by size:
1551 435 431 246 189 169 105
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 2223 2364 3126
Size: 168 2055 141 762
Fragments arranged by size:
2055 762 168 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 1348 2008 2152 2218 2458 2587 3126
Size: 1031 317 660 144 66 240 129 539
Fragments arranged by size:
1031 660 539 317 240 144 129 66
Bpu10I CC'TnA_GC
Cuts at: 0 2069 2500 2600 2840 3126
Size: 2069 431 100 240 286
Fragments arranged by size:
2069 431 286 240 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 823 1459 3126
Size: 823 636 1667
Fragments arranged by size:
1667 823 636
BsaI GGTCTCn'nnnn_
Cuts at: 0 1358 3126
Size: 1358 1768
BsaAI yAC'GTr
Cuts at: 0 288 297 1149 1821 3126
Size: 288 9 852 672 1305
Fragments arranged by size:
1305 852 672 288 9
BsaBI GATnn'nnATC
Cuts at: 0 223 1186 3126
Size: 223 963 1940
Fragments arranged by size:
1940 963 223
BsaHI Gr'CG_yC
Cuts at: 0 1065 2591 2700 3010 3126
Size: 1065 1526 109 310 116
Fragments arranged by size:
1526 1065 310 116 109
BsaWI w'CCGG_w
Cuts at: 0 3056 3126
Size: 3056 70
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 355 385 507 537 3126
Size: 355 30 122 30 2589
Fragments arranged by size:
2589 355 122 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 528 574 1723 1882 2379 2647 2937 2976
Size: 528 46 1149 159 497 268 290 39
Cuts at: 2976 3126
Size: 150
Fragments arranged by size:
1149 528 497 290 268 159 150 46
39
BseYI C'CCAG_C
Cuts at: 0 1278 1756 2419 2857 3126
Size: 1278 478 663 438 269
Fragments arranged by size:
1278 663 478 438 269
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 594 2674 3126
Size: 594 2080 452
Fragments arranged by size:
2080 594 452
BsiEI CG_ry'CG
Cuts at: 0 177 1809 2528 3126
Size: 177 1632 719 598
Fragments arranged by size:
1632 719 598 177
BsiHKAI G_wGCw'C
Cuts at: 0 119 293 1949 2075 2506 2999 3126
Size: 119 174 1656 126 431 493 127
Fragments arranged by size:
1656 493 431 174 127 126 119
BsmI GAATG_Cn'
Cuts at: 0 3040 3126
Size: 3040 86
Bsp1286I G_dGCh'C
Cuts at: 0 119 269 293 1265 1429 1874 1949 1976
Size: 119 150 24 972 164 445 75 27
Cuts at: 1976 2047 2075 2325 2416 2506 2816 2824 2848
Size: 71 28 250 91 90 310 8 24
Cuts at: 2848 2886 2897 2909 2999 3126
Size: 38 11 12 90 127
Fragments arranged by size:
972 445 310 250 164 150 127 119
91 90 90 75 71 38 28 27
24 24 12 11 8
BspEI T'CCGG_A
Cuts at: 0 3056 3126
Size: 3056 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 551 1958 3126
Size: 536 15 1407 1168
Fragments arranged by size:
1407 1168 536 15
BsrBI CCG'CTC
Cuts at: 0 2014 2292 2387 2604 2979 3126
Size: 2014 278 95 217 375 147
Fragments arranged by size:
2014 375 278 217 147 95
BsrDI GCAATG_nn'
Cuts at: 0 707 1052 1529 3126
Size: 707 345 477 1597
Fragments arranged by size:
1597 707 477 345
BsrFI r'CCGG_y
Cuts at: 0 587 807 2664 2929 3126
Size: 587 220 1857 265 197
Fragments arranged by size:
1857 587 265 220 197
BsrGI T'GTAC_A
Cuts at: 0 2366 3126
Size: 2366 760
BssSI C'ACGA_G
Cuts at: 0 142 972 3126
Size: 142 830 2154
Fragments arranged by size:
2154 830 142
BstAPI GCAn_nnn'nTGC
Cuts at: 0 288 3126
Size: 288 2838
BstEII G'GTnAC_C
Cuts at: 0 810 1719 3126
Size: 810 909 1407
Fragments arranged by size:
1407 909 810
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1357 2037 3126
Size: 1357 680 1089
Fragments arranged by size:
1357 1089 680
BstYI r'GATC_y
Cuts at: 0 2870 3053 3126
Size: 2870 183 73
Fragments arranged by size:
2870 183 73
Bsu36I CC'TnA_GG
Cuts at: 0 1706 2391 2723 3126
Size: 1706 685 332 403
Fragments arranged by size:
1706 685 403 332
BtgI C'CryG_G
Cuts at: 0 110 171 177 476 997 1253 1266 1374
Size: 110 61 6 299 521 256 13 108
Cuts at: 1374 2099 2336 2673 2917 3014 3078 3126
Size: 725 237 337 244 97 64 48
Fragments arranged by size:
725 521 337 299 256 244 237 110
108 97 64 61 48 13 6
BtsI GCAGTG_nn'
Cuts at: 0 48 386 1427 1852 2589 3126
Size: 48 338 1041 425 737 537
Fragments arranged by size:
1041 737 537 425 338 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 78 113 1020 1055 1863 1898 2106 2141
Size: 78 35 907 35 808 35 208 35
Cuts at: 2141 3126
Size: 985
Fragments arranged by size:
985 907 808 208 78 35 35 35
35
DraIII CAC_nnn'GTG
Cuts at: 0 2433 3126
Size: 2433 693
EaeI y'GGCC_r
Cuts at: 0 174 221 305 1730 1806 2330 2525 2621
Size: 174 47 84 1425 76 524 195 96
Cuts at: 2621 3075 3126
Size: 454 51
Fragments arranged by size:
1425 524 454 195 174 96 84 76
51 47
EagI C'GGCC_G
Cuts at: 0 174 1806 2525 3126
Size: 174 1632 719 601
Fragments arranged by size:
1632 719 601 174
EarI CTCTTCn'nnn_
Cuts at: 0 124 2464 2633 2764 3126
Size: 124 2340 169 131 362
Fragments arranged by size:
2340 362 169 131 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1674 2317 3126
Size: 1674 643 809
Fragments arranged by size:
1674 809 643
EcoICRI GAG'CTC
Cuts at: 0 2073 2504 2997 3126
Size: 2073 431 493 129
Fragments arranged by size:
2073 493 431 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1031 1348 2008 2152 2218 2458 2587 3126
Size: 1031 317 660 144 66 240 129 539
Fragments arranged by size:
1031 660 539 317 240 144 129 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1348 3126
Size: 1348 1778
EcoO109I rG'GnC_Cy
Cuts at: 0 681 1196 1503 2256 2844 2845 3126
Size: 681 515 307 753 588 1 281
Fragments arranged by size:
753 681 588 515 307 281 1
EcoRI G'AATT_C
Cuts at: 0 843 2734 3126
Size: 843 1891 392
Fragments arranged by size:
1891 843 392
FseI GG_CCGG'CC
Cuts at: 0 2668 3126
Size: 2668 458
HaeII r_GCGC'y
Cuts at: 0 30 1068 1248 2689 2703 3126
Size: 30 1038 180 1441 14 423
Fragments arranged by size:
1441 1038 423 180 30 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 628 648 660 1004 1036 2344 2376
Size: 616 12 20 12 344 32 1308 32
Cuts at: 2376 2521 2553 2932 2964 3126
Size: 145 32 379 32 162
Fragments arranged by size:
1308 616 379 344 162 145 32 32
32 32 20 12 12
KasI G'GCGC_C
Cuts at: 0 1064 2699 3126
Size: 1064 1635 427
Fragments arranged by size:
1635 1064 427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1492 1676 3126
Size: 725 767 184 1450
Fragments arranged by size:
1450 767 725 184
MscI TGG'CCA
Cuts at: 0 223 307 1732 2332 3077 3126
Size: 223 84 1425 600 745 49
Fragments arranged by size:
1425 745 600 223 84 49
MslI CAynn'nnrTG
Cuts at: 0 313 1819 2809 3126
Size: 313 1506 990 317
Fragments arranged by size:
1506 990 317 313
MspA1I CmG'CkG
Cuts at: 0 179 578 735 741 999 1306 1376 1599
Size: 179 399 157 6 258 307 70 223
Cuts at: 1599 2675 3126
Size: 1076 451
Fragments arranged by size:
1076 451 399 307 258 223 179 157
70 6
NaeI GCC'GGC
Cuts at: 0 589 2666 2931 3126
Size: 589 2077 265 195
Fragments arranged by size:
2077 589 265 195
NarI GG'CG_CC
Cuts at: 0 1065 2700 3126
Size: 1065 1635 426
Fragments arranged by size:
1635 1065 426
NcoI C'CATG_G
Cuts at: 0 2336 3126
Size: 2336 790
NgoMIV G'CCGG_C
Cuts at: 0 587 2664 2929 3126
Size: 587 2077 265 197
Fragments arranged by size:
2077 587 265 197
NsiI A_TGCA'T
Cuts at: 0 1687 3126
Size: 1687 1439
NspI r_CATG'y
Cuts at: 0 243 888 3126
Size: 243 645 2238
Fragments arranged by size:
2238 645 243
PasI CC'CwG_GG
Cuts at: 0 1416 2577 3126
Size: 1416 1161 549
Fragments arranged by size:
1416 1161 549
PflMI CCAn_nnn'nTGG
Cuts at: 0 230 578 1074 1416 3126
Size: 230 348 496 342 1710
Fragments arranged by size:
1710 496 348 342 230
PfoI T'CCnGG_A
Cuts at: 0 677 1073 1622 3126
Size: 677 396 549 1504
Fragments arranged by size:
1504 677 549 396
PmlI CAC'GTG
Cuts at: 0 1821 3126
Size: 1821 1305
PpuMI rG'GwC_Cy
Cuts at: 0 681 1196 2256 3126
Size: 681 515 1060 870
Fragments arranged by size:
1060 870 681 515
PspOMI G'GGCC_C
Cuts at: 0 2844 3126
Size: 2844 282
PstI C_TGCA'G
Cuts at: 0 1637 1842 2028 2148 2312 3126
Size: 1637 205 186 120 164 814
Fragments arranged by size:
1637 814 205 186 164 120
PvuII CAG'CTG
Cuts at: 0 578 1306 1599 3126
Size: 578 728 293 1527
Fragments arranged by size:
1527 728 578 293
SacI G_AGCT'C
Cuts at: 0 2075 2506 2999 3126
Size: 2075 431 493 127
Fragments arranged by size:
2075 493 431 127
SacII CC_GC'GG
Cuts at: 0 180 1000 1377 2676 3126
Size: 180 820 377 1299 450
Fragments arranged by size:
1299 820 450 377 180
SanDI GG'GwC_CC
Cuts at: 0 1196 3126
Size: 1196 1930
SapI GCTCTTCn'nnn_
Cuts at: 0 124 3126
Size: 124 3002
ScaI AGT'ACT
Cuts at: 0 364 3126
Size: 364 2762
SexAI A'CCwGG_T
Cuts at: 0 91 161 2900 3126
Size: 91 70 2739 226
Fragments arranged by size:
2739 226 91 70
SfcI C'TryA_G
Cuts at: 0 1633 1769 1838 2024 2144 2308 3126
Size: 1633 136 69 186 120 164 818
Fragments arranged by size:
1633 818 186 164 136 120 69
SfoI GGC'GCC
Cuts at: 0 1066 2701 3126
Size: 1066 1635 425
Fragments arranged by size:
1635 1066 425
SmaI CCC'GGG
Cuts at: 0 6 3126
Size: 6 3120
SmlI C'TyrA_G
Cuts at: 0 838 1474 3126
Size: 838 636 1652
Fragments arranged by size:
1652 838 636
SphI G_CATG'C
Cuts at: 0 243 3126
Size: 243 2883
StuI AGG'CCT
Cuts at: 0 148 1354 1906 3126
Size: 148 1206 552 1220
Fragments arranged by size:
1220 1206 552 148
StyI C'CwwG_G
Cuts at: 0 1208 1535 2030 2336 3126
Size: 1208 327 495 306 790
Fragments arranged by size:
1208 790 495 327 306
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 953 3126
Size: 953 2173
TatI w'GTAC_w
Cuts at: 0 362 1310 2366 3126
Size: 362 948 1056 760
Fragments arranged by size:
1056 948 760 362
Tth111I GACn'n_nGTC
Cuts at: 0 52 1102 1200 1573 3126
Size: 52 1050 98 373 1553
Fragments arranged by size:
1553 1050 373 98 52
XmaI C'CCGG_G
Cuts at: 0 4 3126
Size: 4 3122
Enzymes that do cut:
AarI AccI AhdI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI
HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI
SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BglII BmgBI BmtI BsiWI BsmBI BspHI BssHII
BstBI BstZ17I BtgZI ClaI DraI DrdI EcoRV FalI
FspI FspAI HincII HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI PacI PciI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TstI XbaI XcmI XhoI XmnI ZraI