(Circular) (Six-base) MAPSORT of: pFN21ASDA0658.seq Check: 6748 from: 1 to: 6508 pFN21ASDA0658 6508 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3574 3851 1937 Size: 1637 277 4594 Fragments arranged by size: 4594 1637 277 Acc65I G'GTAC_C Cuts at: 3829 3829 Size: 6508 AclI AA'CG_TT Cuts at: 1563 4635 5008 1563 Size: 3072 373 3063 Fragments arranged by size: 3072 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2231 2706 4571 5955 6223 1632 Size: 599 475 1865 1384 268 1917 Fragments arranged by size: 1917 1865 1384 599 475 268 AfeI AGC'GCT Cuts at: 2534 2534 Size: 6508 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 2325 5407 6384 1203 Size: 1122 3082 977 1327 Fragments arranged by size: 3082 1327 1122 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 2579 3472 1184 Size: 948 447 893 4220 Fragments arranged by size: 4220 948 893 447 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2660 2692 2789 2821 2660 Size: 32 97 32 6347 Fragments arranged by size: 6347 97 32 32 AlwNI CAG_nnn'CTG Cuts at: 2254 4251 5823 2254 Size: 1997 1572 2939 Fragments arranged by size: 2939 1997 1572 ApaI G_GGCC'C Cuts at: 3757 3757 Size: 6508 ApaLI G'TGCA_C Cuts at: 2096 4565 5721 2096 Size: 2469 1156 2883 Fragments arranged by size: 2883 2469 1156 ApoI r'AATT_y Cuts at: 1493 2461 3817 4043 4195 6304 1493 Size: 968 1356 226 152 2109 1697 Fragments arranged by size: 2109 1697 1356 968 226 152 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 3634 3756 3833 1870 Size: 73 1691 122 77 4545 Fragments arranged by size: 4545 1691 122 77 73 BamHI G'GATC_C Cuts at: 3135 3838 4205 3135 Size: 703 367 5438 Fragments arranged by size: 5438 703 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 3829 5284 618 Size: 325 217 627 275 1767 1455 1842 Fragments arranged by size: 1842 1767 1455 627 325 275 217 BanII G_rGCy'C Cuts at: 1391 3420 3630 3757 3827 1391 Size: 2029 210 127 70 4072 Fragments arranged by size: 4072 2029 210 127 70 BbeI G_GCGC'C Cuts at: 2066 2066 Size: 6508 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3609 928 Size: 971 1710 3827 Fragments arranged by size: 3827 1710 971 BbvCI CC'TCA_GC Cuts at: 2831 3072 2831 Size: 241 6267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4699 4733 1903 Size: 34 2762 34 3678 Fragments arranged by size: 3678 2762 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 1548 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2435 2826 3068 Size: 107 122 71 631 605 763 391 242 Cuts at: 3068 3327 3645 3761 5119 136 Size: 259 318 116 1358 1525 Fragments arranged by size: 1525 1358 763 631 605 391 318 259 242 122 116 107 71 BglII A'GATC_T Cuts at: 6503 6503 Size: 6508 BlpI GC'TnA_GC Cuts at: 3925 3925 Size: 6508 Bme1580I G_kGCm'C Cuts at: 1790 2100 2658 3757 4569 5725 1790 Size: 310 558 1099 812 1156 2573 Fragments arranged by size: 2573 1156 1099 812 558 310 BmgBI CAC'GTC Cuts at: 1318 2326 1318 Size: 1008 5500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 3675 4239 Size: 416 53 83 399 24 1928 330 564 Cuts at: 4239 5197 442 Size: 958 1753 Fragments arranged by size: 1928 1753 958 564 416 399 330 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6508 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3649 3649 Size: 6508 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2684 5168 1239 Size: 1445 2484 2579 Fragments arranged by size: 2579 2484 1445 Bpu10I CC'TnA_GC Cuts at: 2831 3072 2831 Size: 241 6267 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4633 5498 5796 4633 Size: 865 298 5345 Fragments arranged by size: 5345 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2636 2779 3497 5171 882 Size: 617 1137 143 718 1674 2219 Fragments arranged by size: 2219 1674 1137 718 617 143 BsaAI yAC'GTr Cuts at: 493 1204 3805 4299 493 Size: 711 2601 494 2702 Fragments arranged by size: 2702 2601 711 494 BsaBI GATnn'nnATC Cuts at: 6502 6502 Size: 6508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2063 4315 4697 5332 Size: 53 83 186 936 530 2252 382 635 Cuts at: 5332 275 Size: 1451 Fragments arranged by size: 2252 1451 936 635 530 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4941 5339 5613 5760 6171 4941 Size: 398 274 147 411 5278 Fragments arranged by size: 5278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2660 2690 2789 2819 1178 Size: 30 165 30 1257 30 99 30 4867 Fragments arranged by size: 4867 1257 165 99 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2200 2203 2681 2730 1186 Size: 1014 3 478 49 4964 Fragments arranged by size: 4964 1014 478 49 3 BseYI C'CCAG_C Cuts at: 2671 3198 3629 3688 5711 6207 2671 Size: 527 431 59 2023 496 2972 Fragments arranged by size: 2972 2023 527 496 431 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3681 1163 Size: 573 1945 3990 Fragments arranged by size: 3990 1945 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2151 3958 4719 5747 1540 Size: 275 186 150 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 150 BsiHKAI G_wGCw'C Cuts at: 2100 4569 4654 5725 2100 Size: 2469 85 1071 2883 Fragments arranged by size: 2883 2469 1071 85 BsmI GAATG_Cn' Cuts at: 3430 4019 4112 3430 Size: 589 93 5826 Fragments arranged by size: 5826 589 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2658 3420 3630 3757 3827 4569 Size: 399 310 558 762 210 127 70 742 Cuts at: 4569 4654 5725 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 762 742 558 399 310 210 127 85 70 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 2418 3108 4397 6127 2418 Size: 690 1289 1730 2799 Fragments arranged by size: 2799 1730 1289 690 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2850 3384 3863 844 Size: 2006 534 479 3489 Fragments arranged by size: 3489 2006 534 479 BsrBI CCG'CTC Cuts at: 1394 1823 3207 4395 1394 Size: 429 1384 1188 3507 Fragments arranged by size: 3507 1384 1188 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5003 5177 65 Size: 1183 3755 174 1396 Fragments arranged by size: 3755 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3510 5152 5339 1700 Size: 110 1700 1642 187 2869 Fragments arranged by size: 2869 1700 1642 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6508 BssHII G'CGCG_C Cuts at: 1925 2162 1925 Size: 237 6271 BssSI C'ACGA_G Cuts at: 4568 5580 4568 Size: 1012 5496 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6508 BstBI TT'CG_AA Cuts at: 2007 5312 2007 Size: 3305 3203 BstEII G'GTnAC_C Cuts at: 1184 2587 1184 Size: 1403 5105 BstXI CCAn_nnnn'nTGG Cuts at: 2706 2706 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2622 3030 3135 3838 4205 4590 4607 6059 Size: 650 408 105 703 367 385 17 1452 Cuts at: 6059 6145 6157 6503 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 703 650 408 385 367 346 105 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3051 3051 Size: 6508 BtgI C'CryG_G Cuts at: 513 1065 2022 2195 3159 3178 3479 513 Size: 552 957 173 964 19 301 3542 Fragments arranged by size: 3542 964 957 552 301 173 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 3292 3310 3664 4024 4818 4838 3292 Size: 18 354 360 794 20 4962 Fragments arranged by size: 4962 794 360 354 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6473 DraI TTT'AAA Cuts at: 3812 3812 Size: 6508 DraIII CAC_nnn'GTG Cuts at: 3619 3734 3619 Size: 115 6393 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3191 5515 809 Size: 507 1875 2324 1802 Fragments arranged by size: 2324 1875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2427 2814 Size: 54 1425 50 275 18 318 279 387 Cuts at: 2814 3060 3334 3955 4844 6473 8 Size: 246 274 621 889 1629 43 Fragments arranged by size: 1629 1425 889 621 387 318 279 275 274 246 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2148 3955 1537 Size: 275 336 1807 4090 Fragments arranged by size: 4090 1807 336 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2095 2876 4438 1353 Size: 742 781 1562 3423 Fragments arranged by size: 3423 1562 781 742 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5095 5469 5615 387 Size: 1064 138 3506 374 146 1280 Fragments arranged by size: 3506 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2231 2684 2706 4571 5168 5955 6223 Size: 393 599 453 22 1865 597 787 268 Cuts at: 6223 1239 Size: 1524 Fragments arranged by size: 1865 1524 787 599 597 453 393 268 22 EcoO109I rG'GnC_Cy Cuts at: 2890 3055 3184 2890 Size: 165 129 6214 Fragments arranged by size: 6214 165 129 EcoRI G'AATT_C Cuts at: 2461 3817 4195 2461 Size: 1356 378 4774 Fragments arranged by size: 4774 1356 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3103 3103 Size: 6508 FspI TGC'GCA Cuts at: 5014 5014 Size: 6508 HaeII r_GCGC'y Cuts at: 1678 2066 2072 2088 2536 5655 1678 Size: 388 6 16 448 3119 2531 Fragments arranged by size: 3119 2531 448 388 16 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2072 2104 2713 2745 5149 Size: 32 135 32 500 32 609 32 2404 Cuts at: 5149 5181 5223 5255 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2404 609 500 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3493 3852 4098 669 Size: 988 281 1555 359 246 3079 Fragments arranged by size: 3079 1555 988 359 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6508 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KasI G'GCGC_C Cuts at: 2062 2062 Size: 6508 KpnI G_GTAC'C Cuts at: 3833 3833 Size: 6508 MfeI C'AATT_G Cuts at: 4107 4107 Size: 6508 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5622 5806 1305 Size: 4317 184 2007 Fragments arranged by size: 4317 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2429 2816 3062 10 Size: 54 1425 343 597 387 246 3456 Fragments arranged by size: 3456 1425 597 387 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3467 4466 4825 4984 518 Size: 648 1257 1044 999 359 159 2042 Fragments arranged by size: 2042 1257 1044 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2051 2702 3180 3198 3267 3924 4601 5749 Size: 445 651 478 18 69 657 677 1148 Cuts at: 5749 5994 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 657 651 478 445 245 69 18 NaeI GCC'GGC Cuts at: 3512 3512 Size: 6508 NarI GG'CG_CC Cuts at: 2063 2063 Size: 6508 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4999 Fragments arranged by size: 4999 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NgoMIV G'CCGG_C Cuts at: 3510 3510 Size: 6508 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6508 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 3158 3866 5411 1132 Size: 97 1929 708 1545 2229 Fragments arranged by size: 2229 1929 1545 708 97 PasI CC'CwG_GG Cuts at: 1348 3036 1348 Size: 1688 4820 PciI A'CATG_T Cuts at: 5407 5407 Size: 6508 PflMI CCAn_nnn'nTGG Cuts at: 1108 2602 3145 1108 Size: 1494 543 4471 Fragments arranged by size: 4471 1494 543 PfoI T'CCnGG_A Cuts at: 2625 3234 2625 Size: 609 5899 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2660 2692 2789 2821 4577 4609 6090 6122 2660 Size: 32 97 32 1756 32 1481 32 3046 Fragments arranged by size: 3046 1756 1481 97 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2890 3184 2890 Size: 294 6214 PshAI GACnn'nnGTC Cuts at: 1613 3609 1613 Size: 1996 4512 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PspOMI G'GGCC_C Cuts at: 3753 3753 Size: 6508 PspXI vC'TCGA_Gb Cuts at: 3634 3634 Size: 6508 PstI C_TGCA'G Cuts at: 830 2615 3287 3860 830 Size: 1785 672 573 3478 Fragments arranged by size: 3478 1785 672 573 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6508 PvuII CAG'CTG Cuts at: 2051 2702 3198 3267 2051 Size: 651 496 69 5292 Fragments arranged by size: 5292 651 496 69 SacII CC_GC'GG Cuts at: 3181 3181 Size: 6508 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SbfI CC_TGCA'GG Cuts at: 3860 3860 Size: 6508 ScaI AGT'ACT Cuts at: 4756 6468 4756 Size: 1712 4796 SexAI A'CCwGG_T Cuts at: 3730 3730 Size: 6508 SfcI C'TryA_G Cuts at: 826 1045 2611 3283 3856 4991 5672 5863 826 Size: 219 1566 672 573 1135 681 191 1471 Fragments arranged by size: 1566 1471 1135 681 672 573 219 191 SfoI GGC'GCC Cuts at: 2064 2064 Size: 6508 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 3758 3835 1872 Size: 1886 77 4545 Fragments arranged by size: 4545 1886 77 SmlI C'TyrA_G Cuts at: 820 1017 1943 3634 4612 5513 5775 820 Size: 197 926 1691 978 901 262 1553 Fragments arranged by size: 1691 1553 978 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3805 4299 493 Size: 3312 494 2702 Fragments arranged by size: 3312 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3158 3866 1132 Size: 2026 708 3774 Fragments arranged by size: 3774 2026 708 SspI AAT'ATT Cuts at: 5 52 4432 5 Size: 47 4380 2081 Fragments arranged by size: 4380 2081 47 StuI AGG'CCT Cuts at: 1517 2765 2829 1517 Size: 1248 64 5196 Fragments arranged by size: 5196 1248 64 StyI C'CwwG_G Cuts at: 513 1065 2022 2333 2430 3092 3947 513 Size: 552 957 311 97 662 855 3074 Fragments arranged by size: 3074 957 855 662 552 311 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4535 4552 4705 4890 5335 325 Size: 1255 2955 17 153 185 445 1498 Fragments arranged by size: 2955 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4754 6466 96 Size: 274 80 33 51 1443 2777 1712 138 Fragments arranged by size: 2777 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6468 6500 6468 Size: 32 6476 Tth111I GACn'n_nGTC Cuts at: 1316 2694 3570 1316 Size: 1378 876 4254 Fragments arranged by size: 4254 1378 876 XbaI T'CTAG_A Cuts at: 3844 3844 Size: 6508 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2422 2575 2599 2809 3706 2422 Size: 153 24 210 897 5224 Fragments arranged by size: 5224 897 210 153 24 XhoI C'TCGA_G Cuts at: 1943 3634 1943 Size: 1691 4817 XmaI C'CCGG_G Cuts at: 1870 3756 3833 1870 Size: 1886 77 4545 Fragments arranged by size: 4545 1886 77 XmnI GAAnn'nnTTC Cuts at: 3392 4637 3392 Size: 1245 5263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI