Restriction map for
pFN21ASDA0655
(Linear) (Six-base) MAPSORT of: KIAA0655.seq Check: 6838 from: 1 to: 3204
KIAA0655.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1482 1949 2179 2940 3204
Size: 1482 467 230 761 264
Fragments arranged by size:
1482 761 467 264 230
AccI GT'mk_AC
Cuts at: 0 395 2348 3204
Size: 395 1953 856
Fragments arranged by size:
1953 856 395
Acc65I G'GTAC_C
Cuts at: 0 320 3204
Size: 320 2884
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 580 1171 1369 2266 2947 3204
Size: 580 591 198 897 681 257
Fragments arranged by size:
897 681 591 580 257 198
AgeI A'CCGG_T
Cuts at: 0 752 3204
Size: 752 2452
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1843 1875 3204
Size: 1843 32 1329
Fragments arranged by size:
1843 1329 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1293 1725 1833 1845 2034 2232 2298 3204
Size: 1293 432 108 12 189 198 66 906
Fragments arranged by size:
1293 906 432 198 189 108 66 12
ApaI G_GGCC'C
Cuts at: 0 974 1983 2190 3204
Size: 974 1009 207 1014
Fragments arranged by size:
1014 1009 974 207
ApaLI G'TGCA_C
Cuts at: 0 1378 3204
Size: 1378 1826
ApoI r'AATT_y
Cuts at: 0 2578 3204
Size: 2578 626
AvaI C'yCGr_G
Cuts at: 0 847 916 2189 2436 3204
Size: 847 69 1273 247 768
Fragments arranged by size:
1273 847 768 247 69
BamHI G'GATC_C
Cuts at: 0 2465 3204
Size: 2465 739
BanI G'GyrC_C
Cuts at: 0 290 320 2119 3204
Size: 290 30 1799 1085
Fragments arranged by size:
1799 1085 290 30
BanII G_rGCy'C
Cuts at: 0 974 1370 1639 1983 2190 2196 2204 2708
Size: 974 396 269 344 207 6 8 504
Cuts at: 2708 3204
Size: 496
Fragments arranged by size:
974 504 496 396 344 269 207 8
6
BbsI GAAGACnn'nnnn_
Cuts at: 0 2980 3204
Size: 2980 224
BbvCI CC'TCA_GC
Cuts at: 0 876 1255 3204
Size: 876 379 1949
Fragments arranged by size:
1949 876 379
BciVI GTATCCnnnnn_n'
Cuts at: 0 618 2094 3204
Size: 618 1476 1110
Fragments arranged by size:
1476 1110 618
BglI GCCn_nnn'nGGC
Cuts at: 0 1330 1987 2065 2629 2740 3204
Size: 1330 657 78 564 111 464
Fragments arranged by size:
1330 657 564 464 111 78
BglII A'GATC_T
Cuts at: 0 419 3204
Size: 419 2785
BlpI GC'TnA_GC
Cuts at: 0 34 887 1633 1750 3204
Size: 34 853 746 117 1454
Fragments arranged by size:
1454 853 746 117 34
Bme1580I G_kGCm'C
Cuts at: 0 172 293 974 1382 1983 2190 3204
Size: 172 121 681 408 601 207 1014
Fragments arranged by size:
1014 681 601 408 207 172 121
BmgBI CAC'GTC
Cuts at: 0 264 3204
Size: 264 2940
BmrI ACTGGGnnnn_n'
Cuts at: 0 438 2022 3204
Size: 438 1584 1182
Fragments arranged by size:
1584 1182 438
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1653 1835 3204
Size: 1653 182 1369
Fragments arranged by size:
1653 1369 182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 481 502 1045 1129 1144 1192 1204
Size: 79 402 21 543 84 15 48 12
Cuts at: 1204 1291 1561 1582 2032 2218 2470 2983 2989
Size: 87 270 21 450 186 252 513 6
Cuts at: 2989 3001 3074 3204
Size: 12 73 130
Fragments arranged by size:
543 513 450 402 270 252 186 130
87 84 79 73 48 21 21 15
12 12 6
Bpu10I CC'TnA_GC
Cuts at: 0 876 1255 1603 2794 3204
Size: 876 379 348 1191 410
Fragments arranged by size:
1191 876 410 379 348
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1534 3204
Size: 418 1116 1670
Fragments arranged by size:
1670 1116 418
BsaI GGTCTCn'nnnn_
Cuts at: 0 341 3204
Size: 341 2863
BsaAI yAC'GTr
Cuts at: 0 3027 3204
Size: 3027 177
BsaHI Gr'CG_yC
Cuts at: 0 2051 3204
Size: 2051 1153
BsaWI w'CCGG_w
Cuts at: 0 752 3204
Size: 752 2452
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1495 1525 2023 2053 3047 3077 3204
Size: 1495 30 498 30 994 30 127
Fragments arranged by size:
1495 994 498 127 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 934 952 1201 1315 1462 1537 2029 2344
Size: 934 18 249 114 147 75 492 315
Cuts at: 2344 2716 3073 3204
Size: 372 357 131
Fragments arranged by size:
934 492 372 357 315 249 147 131
114 75 18
BseYI C'CCAG_C
Cuts at: 0 229 493 1130 1250 1265 2286 2334 2639
Size: 229 264 637 120 15 1021 48 305
Cuts at: 2639 2744 3000 3034 3076 3130 3183 3204
Size: 105 256 34 42 54 53 21
Fragments arranged by size:
1021 637 305 264 256 229 120 105
54 53 48 42 34 21 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1921 2463 3204
Size: 1921 542 741
Fragments arranged by size:
1921 741 542
BsiEI CG_ry'CG
Cuts at: 0 2157 3204
Size: 2157 1047
BsiHKAI G_wGCw'C
Cuts at: 0 893 1370 1382 1639 2008 2204 2708 3204
Size: 893 477 12 257 369 196 504 496
Fragments arranged by size:
893 504 496 477 369 257 196 12
BsmI GAATG_Cn'
Cuts at: 0 1170 3204
Size: 1170 2034
BsmBI CGTCTCn'nnnn_
Cuts at: 0 278 2938 3204
Size: 278 2660 266
Fragments arranged by size:
2660 278 266
Bsp1286I G_dGCh'C
Cuts at: 0 172 293 893 974 1370 1382 1639 1983
Size: 172 121 600 81 396 12 257 344
Cuts at: 1983 2008 2190 2196 2204 2708 3204
Size: 25 182 6 8 504 496
Fragments arranged by size:
600 504 496 396 344 257 182 172
121 81 25 12 8 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1482 1715 1949 2179 2940 3204
Size: 1482 233 234 230 761 264
Fragments arranged by size:
1482 761 264 234 233 230
BsrBI CCG'CTC
Cuts at: 0 1100 1281 2552 3204
Size: 1100 181 1271 652
Fragments arranged by size:
1271 1100 652 181
BsrFI r'CCGG_y
Cuts at: 0 21 752 900 1799 3204
Size: 21 731 148 899 1405
Fragments arranged by size:
1405 899 731 148 21
BssHII G'CGCG_C
Cuts at: 0 1333 1589 3204
Size: 1333 256 1615
Fragments arranged by size:
1615 1333 256
BssSI C'ACGA_G
Cuts at: 0 178 760 1240 2722 3193 3204
Size: 178 582 480 1482 471 11
Fragments arranged by size:
1482 582 480 471 178 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1833 1845 2180 2232 3204
Size: 1833 12 335 52 972
Fragments arranged by size:
1833 972 335 52 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2634 2655 2847 3204
Size: 2634 21 192 357
Fragments arranged by size:
2634 357 192 21
BstYI r'GATC_y
Cuts at: 0 419 833 2465 3204
Size: 419 414 1632 739
Fragments arranged by size:
1632 739 419 414
Bsu36I CC'TnA_GG
Cuts at: 0 621 778 3204
Size: 621 157 2426
Fragments arranged by size:
2426 621 157
BtgI C'CryG_G
Cuts at: 0 452 977 1325 1892 2342 2894 3204
Size: 452 525 348 567 450 552 310
Fragments arranged by size:
567 552 525 452 450 348 310
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 585 1125 3204
Size: 585 540 2079
Fragments arranged by size:
2079 585 540
BtsI GCAGTG_nn'
Cuts at: 0 1871 3080 3204
Size: 1871 1209 124
Fragments arranged by size:
1871 1209 124
DraIII CAC_nnn'GTG
Cuts at: 0 3027 3204
Size: 3027 177
EaeI y'GGCC_r
Cuts at: 0 596 2363 3065 3204
Size: 596 1767 702 139
Fragments arranged by size:
1767 702 596 139
EarI CTCTTCn'nnn_
Cuts at: 0 1012 1072 1147 1522 3204
Size: 1012 60 75 375 1682
Fragments arranged by size:
1682 1012 375 75 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1225 2046 3204
Size: 1225 821 1158
Fragments arranged by size:
1225 1158 821
EcoICRI GAG'CTC
Cuts at: 0 1368 1637 2202 2706 3204
Size: 1368 269 565 504 498
Fragments arranged by size:
1368 565 504 498 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 481 502 580 1045 1129 1144 1171
Size: 79 402 21 78 465 84 15 27
Cuts at: 1171 1192 1204 1291 1369 1561 1582 2032 2218
Size: 21 12 87 78 192 21 450 186
Cuts at: 2218 2266 2470 2947 2983 2989 3001 3074 3204
Size: 48 204 477 36 6 12 73 130
Fragments arranged by size:
477 465 450 402 204 192 186 130
87 84 79 78 78 73 48 36
27 21 21 21 15 12 12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 271 649 1297 2014 2491 2533 3204
Size: 271 378 648 717 477 42 671
Fragments arranged by size:
717 671 648 477 378 271 42
EcoO109I rG'GnC_Cy
Cuts at: 0 129 276 853 924 970 971 1056 1212
Size: 129 147 577 71 46 1 85 156
Cuts at: 1212 1290 1599 1979 2186 3204
Size: 78 309 380 207 1018
Fragments arranged by size:
1018 577 380 309 207 156 147 129
85 78 71 46 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 441 3204
Size: 441 2763
HaeII r_GCGC'y
Cuts at: 0 1750 1880 3204
Size: 1750 130 1324
Fragments arranged by size:
1750 1324 130
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1801 1833 3204
Size: 1801 32 1371
Fragments arranged by size:
1801 1371 32
HincII GTy'rAC
Cuts at: 0 507 2349 3204
Size: 507 1842 855
Fragments arranged by size:
1842 855 507
HindIII A'AGCT_T
Cuts at: 0 562 3204
Size: 562 2642
KpnI G_GTAC'C
Cuts at: 0 324 3204
Size: 324 2880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 827 1487 1607 3204
Size: 606 221 660 120 1597
Fragments arranged by size:
1597 660 606 221 120
MscI TGG'CCA
Cuts at: 0 3067 3204
Size: 3067 137
MslI CAynn'nnrTG
Cuts at: 0 296 365 3204
Size: 296 69 2839
Fragments arranged by size:
2839 296 69
MspA1I CmG'CkG
Cuts at: 0 225 248 318 384 454 493 979 1134
Size: 225 23 70 66 70 39 486 155
Cuts at: 1134 1149 1194 1254 1269 1422 1473 1539 1695
Size: 15 45 60 15 153 51 66 156
Cuts at: 1695 1894 2214 2286 2655 2668 2748 3204
Size: 199 320 72 369 13 80 456
Fragments arranged by size:
486 456 369 320 225 199 156 155
153 80 72 70 70 66 66 60
51 45 39 23 15 15 13
NaeI GCC'GGC
Cuts at: 0 23 1801 3204
Size: 23 1778 1403
Fragments arranged by size:
1778 1403 23
NcoI C'CATG_G
Cuts at: 0 2894 3204
Size: 2894 310
NgoMIV G'CCGG_C
Cuts at: 0 21 1799 3204
Size: 21 1778 1405
Fragments arranged by size:
1778 1405 21
NspI r_CATG'y
Cuts at: 0 810 2244 2997 3204
Size: 810 1434 753 207
Fragments arranged by size:
1434 810 753 207
PflMI CCAn_nnn'nTGG
Cuts at: 0 57 1245 2751 3204
Size: 57 1188 1506 453
Fragments arranged by size:
1506 1188 453 57
PfoI T'CCnGG_A
Cuts at: 0 335 659 2960 3204
Size: 335 324 2301 244
Fragments arranged by size:
2301 335 324 244
PpuMI rG'GwC_Cy
Cuts at: 0 276 853 1056 3204
Size: 276 577 203 2148
Fragments arranged by size:
2148 577 276 203
PshAI GACnn'nnGTC
Cuts at: 0 502 3204
Size: 502 2702
PspOMI G'GGCC_C
Cuts at: 0 970 1979 2186 3204
Size: 970 1009 207 1018
Fragments arranged by size:
1018 1009 970 207
PstI C_TGCA'G
Cuts at: 0 670 1907 2176 2219 2273 2534 2807 3204
Size: 670 1237 269 43 54 261 273 397
Fragments arranged by size:
1237 670 397 273 269 261 54 43
PvuII CAG'CTG
Cuts at: 0 248 384 493 1149 1254 1269 1422 1473
Size: 248 136 109 656 105 15 153 51
Cuts at: 1473 1539 2214 2286 2655 2668 2748 3204
Size: 66 675 72 369 13 80 456
Fragments arranged by size:
675 656 456 369 248 153 136 109
105 80 72 66 51 15 13
SacI G_AGCT'C
Cuts at: 0 1370 1639 2204 2708 3204
Size: 1370 269 565 504 496
Fragments arranged by size:
1370 565 504 496 269
SacII CC_GC'GG
Cuts at: 0 455 980 1895 3204
Size: 455 525 915 1309
Fragments arranged by size:
1309 915 525 455
SalI G'TCGA_C
Cuts at: 0 2347 3204
Size: 2347 857
SanDI GG'GwC_CC
Cuts at: 0 853 3204
Size: 853 2351
SapI GCTCTTCn'nnn_
Cuts at: 0 1147 1522 3204
Size: 1147 375 1682
Fragments arranged by size:
1682 1147 375
SbfI CC_TGCA'GG
Cuts at: 0 1907 2176 2273 2807 3204
Size: 1907 269 97 534 397
Fragments arranged by size:
1907 534 397 269 97
SexAI A'CCwGG_T
Cuts at: 0 1967 3204
Size: 1967 1237
SfcI C'TryA_G
Cuts at: 0 666 1903 2172 2215 2269 2530 2803 3204
Size: 666 1237 269 43 54 261 273 401
Fragments arranged by size:
1237 666 401 273 269 261 54 43
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1987 3204
Size: 1987 1217
SmaI CCC'GGG
Cuts at: 0 2191 3204
Size: 2191 1013
SmlI C'TyrA_G
Cuts at: 0 433 1549 3204
Size: 433 1116 1655
Fragments arranged by size:
1655 1116 433
SphI G_CATG'C
Cuts at: 0 2997 3204
Size: 2997 207
SrfI GCCC'GGGC
Cuts at: 0 2191 3204
Size: 2191 1013
StuI AGG'CCT
Cuts at: 0 1990 2612 3204
Size: 1990 622 592
Fragments arranged by size:
1990 622 592
StyI C'CwwG_G
Cuts at: 0 1568 1991 2042 2627 2762 2894 3204
Size: 1568 423 51 585 135 132 310
Fragments arranged by size:
1568 585 423 310 135 132 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 514 2620 3204
Size: 136 378 2106 584
Fragments arranged by size:
2106 584 378 136
TatI w'GTAC_w
Cuts at: 0 812 3204
Size: 812 2392
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 371 403 1305 1337 3047 3079 3204
Size: 371 32 902 32 1710 32 125
Fragments arranged by size:
1710 902 371 125 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2130 2655 3204
Size: 2130 525 549
Fragments arranged by size:
2130 549 525
XmaI C'CCGG_G
Cuts at: 0 2189 3204
Size: 2189 1015
XmnI GAAnn'nnTTC
Cuts at: 0 787 3204
Size: 787 2417
Enzymes that do cut:
AarI AccI Acc65I AcuI AgeI AloI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BciVI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PflMI PfoI PpuMI PshAI PspOMI PstI
PvuII SacI SacII SalI SanDI SapI SbfI SexAI
SfcI SfiI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AhdI AleI AlfI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI
BfrBI BmtI BsaBI BsiWI BspEI BspHI BsrDI BsrGI
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI
EcoRI EcoRV FseI FspI FspAI HpaI KasI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI
PvuI RsrII ScaI SfoI SgfI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI XhoI ZraI