(Circular) (Six-base) MAPSORT of: pFN21ASDA0654.seq Check: 3924 from: 1 to: 8311 pFN21ASDA0654 8311 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6121 278 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5041 5654 1937 Size: 3104 613 4594 Fragments arranged by size: 4594 3104 613 Acc65I G'GTAC_C Cuts at: 5632 5632 Size: 8311 AclI AA'CG_TT Cuts at: 1563 6438 6811 1563 Size: 4875 373 3063 Fragments arranged by size: 4875 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2360 3422 3824 5207 6374 7758 8026 1632 Size: 728 1062 402 1383 1167 1384 268 1917 Fragments arranged by size: 1917 1384 1383 1167 1062 728 402 268 AfeI AGC'GCT Cuts at: 2954 3353 2954 Size: 399 7912 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8114 AflIII A'CryG_T Cuts at: 1203 4779 7210 8187 1203 Size: 3576 2431 977 1327 Fragments arranged by size: 3576 2431 1327 977 AgeI A'CCGG_T Cuts at: 7142 7142 Size: 8311 AhdI GACnn_n'nnGTC Cuts at: 7040 7040 Size: 8311 AleI CACnn'nnGTG Cuts at: 1166 4550 1166 Size: 3384 4927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2270 2700 4703 1184 Size: 1086 430 2003 4792 Fragments arranged by size: 4792 2003 1086 430 AlwNI CAG_nnn'CTG Cuts at: 2311 3103 3115 3826 3978 4861 5320 6054 7626 Size: 792 12 711 152 883 459 734 1572 Cuts at: 7626 2311 Size: 2996 Fragments arranged by size: 2996 1572 883 792 734 711 459 152 12 ApaI G_GGCC'C Cuts at: 2084 3271 4545 2084 Size: 1187 1274 5850 Fragments arranged by size: 5850 1274 1187 ApaLI G'TGCA_C Cuts at: 6368 7524 6368 Size: 1156 7155 ApoI r'AATT_y Cuts at: 1493 3202 5192 5330 5620 5846 5998 8107 1493 Size: 1709 1990 138 290 226 152 2109 1697 Fragments arranged by size: 2109 1990 1709 1697 290 226 152 138 AseI AT'TA_AT Cuts at: 160 1032 6865 160 Size: 872 5833 1606 Fragments arranged by size: 5833 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 AvaI C'yCGr_G Cuts at: 1870 1943 2449 2573 3137 4829 5568 5636 1870 Size: 73 506 124 564 1692 739 68 4545 Fragments arranged by size: 4545 1692 739 564 506 124 73 68 BamHI G'GATC_C Cuts at: 3613 5641 6008 3613 Size: 2028 367 5916 Fragments arranged by size: 5916 2028 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2834 3583 3602 4060 4085 Size: 325 217 627 1047 749 19 458 25 Cuts at: 4085 4268 4625 5632 7087 618 Size: 183 357 1007 1455 1842 Fragments arranged by size: 1842 1455 1047 1007 749 627 458 357 325 217 183 25 19 BanII G_rGCy'C Cuts at: 1391 2072 2084 2211 3271 3570 3681 3716 4129 Size: 681 12 127 1060 299 111 35 413 Cuts at: 4129 4545 4998 5506 5513 5523 5630 1391 Size: 416 453 508 7 10 107 4072 Fragments arranged by size: 4072 1060 681 508 453 416 413 299 127 111 107 35 12 10 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 7340 BbvCI CC'TCA_GC Cuts at: 4744 4744 Size: 8311 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6502 6536 1903 Size: 34 4565 34 3678 Fragments arranged by size: 4565 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6203 7413 6203 Size: 1210 7101 BclI T'GATC_A Cuts at: 1548 1548 Size: 8311 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 4515 4607 Size: 107 122 71 631 605 395 2448 92 Cuts at: 4607 4634 6922 136 Size: 27 2288 1525 Fragments arranged by size: 2448 2288 1525 631 605 395 122 107 92 71 27 BglII A'GATC_T Cuts at: 8306 8306 Size: 8311 BlpI GC'TnA_GC Cuts at: 2243 3335 5728 2243 Size: 1092 2393 4826 Fragments arranged by size: 4826 2393 1092 Bme1580I G_kGCm'C Cuts at: 1790 2084 2837 3271 3562 3607 4088 4271 4545 Size: 294 753 434 291 45 481 183 274 Cuts at: 4545 5233 6372 7528 1790 Size: 688 1139 1156 2573 Fragments arranged by size: 2573 1156 1139 753 688 481 434 294 291 274 183 45 BmgBI CAC'GTC Cuts at: 1318 2982 3817 5134 1318 Size: 1664 835 1317 4495 Fragments arranged by size: 4495 1664 1317 835 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2437 3795 6042 Size: 416 53 83 399 24 1020 1358 2247 Cuts at: 6042 7000 442 Size: 958 1753 Fragments arranged by size: 2247 1753 1358 1020 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3027 4439 1056 Size: 1971 1412 4928 Fragments arranged by size: 4928 1971 1412 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2242 3418 3697 4744 2242 Size: 1176 279 1047 5809 Fragments arranged by size: 5809 1176 1047 279 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2171 2333 2402 2576 2756 2768 2840 Size: 887 45 162 69 174 180 12 72 Cuts at: 2840 2966 3029 3248 3410 3523 3584 3872 3944 Size: 126 63 219 162 113 61 288 72 Cuts at: 3944 4143 4517 4583 5114 5342 6971 1239 Size: 199 374 66 531 228 1629 2579 Fragments arranged by size: 2579 1629 887 531 374 288 228 219 199 180 174 162 162 126 113 72 72 69 66 63 61 45 12 Bpu10I CC'TnA_GC Cuts at: 2960 3407 4744 2960 Size: 447 1337 6527 Fragments arranged by size: 6527 1337 447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2879 3353 4214 4682 4994 6436 7301 7599 2879 Size: 474 861 468 312 1442 865 298 3591 Fragments arranged by size: 3591 1442 865 861 474 468 312 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2464 3122 3549 4732 4826 5243 6974 Size: 617 965 658 427 1183 94 417 1731 Cuts at: 6974 882 Size: 2219 Fragments arranged by size: 2219 1731 1183 965 658 617 427 417 94 BsaAI yAC'GTr Cuts at: 493 1204 5608 6102 493 Size: 711 4404 494 2702 Fragments arranged by size: 4404 2702 711 494 BsaBI GATnn'nnATC Cuts at: 3500 4673 8305 3500 Size: 1173 3632 3506 Fragments arranged by size: 3632 3506 1173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2994 3090 6118 6500 Size: 53 83 186 936 1461 96 3028 382 Cuts at: 6500 7135 275 Size: 635 1451 Fragments arranged by size: 3028 1461 1451 936 635 382 186 96 83 53 BsaWI w'CCGG_w Cuts at: 5082 5589 6744 7142 7416 7563 7974 5082 Size: 507 1155 398 274 147 411 5419 Fragments arranged by size: 5419 1155 507 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2813 2843 2966 2996 3002 Size: 30 165 30 1410 30 123 30 6 Cuts at: 3002 3023 3032 3053 4790 4820 5330 5360 1178 Size: 21 9 21 1737 30 510 30 4129 Fragments arranged by size: 4129 1737 1410 510 165 123 30 30 30 30 30 30 21 21 9 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2452 2591 2594 2753 2837 2840 2942 3224 Size: 1266 139 3 159 84 3 102 282 Cuts at: 3224 3407 3428 3689 3773 3776 3902 3932 4514 Size: 183 21 261 84 3 126 30 582 Cuts at: 4514 4747 4811 1186 Size: 233 64 4686 Fragments arranged by size: 4686 1266 582 282 261 233 183 159 139 126 102 84 84 64 30 21 3 3 3 BseYI C'CCAG_C Cuts at: 2077 2851 2967 3234 4031 4848 4910 5179 7514 Size: 774 116 267 797 817 62 269 2335 Cuts at: 7514 8010 2077 Size: 496 2378 Fragments arranged by size: 2378 2335 817 797 774 496 269 267 116 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2972 3839 4268 4673 1163 Size: 573 1236 867 429 405 4801 Fragments arranged by size: 4801 1236 867 573 429 405 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5761 6522 7550 1540 Size: 275 186 3760 761 1028 2301 Fragments arranged by size: 3760 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2481 2508 2574 3570 4129 4998 6372 6457 Size: 270 27 66 996 559 869 1374 85 Cuts at: 6457 7528 2211 Size: 1071 2994 Fragments arranged by size: 2994 1374 1071 996 869 559 270 85 66 27 BsmI GAATG_Cn' Cuts at: 5305 5822 5915 5305 Size: 517 93 7701 Fragments arranged by size: 7701 517 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2147 2988 3311 4334 5124 2147 Size: 841 323 1023 790 5334 Fragments arranged by size: 5334 1023 841 790 323 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2084 2211 2481 2508 2574 2837 Size: 399 282 12 127 270 27 66 263 Cuts at: 2837 3271 3562 3570 3607 3681 3716 4088 4129 Size: 434 291 8 37 74 35 372 41 Cuts at: 4129 4271 4545 4998 5233 5506 5513 5523 5630 Size: 142 274 453 235 273 7 10 107 Cuts at: 5630 6372 6457 7528 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 453 434 399 372 291 282 274 273 270 263 235 142 127 107 85 74 66 41 37 35 27 12 10 8 7 BspEI T'CCGG_A Cuts at: 5589 7974 5589 Size: 2385 5926 BspHI T'CATG_A Cuts at: 6200 7930 6200 Size: 1730 6581 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5666 844 Size: 4822 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2539 2752 2932 3214 3487 3697 4182 Size: 429 716 213 180 282 273 210 485 Cuts at: 4182 4623 5098 5155 5217 5400 6198 1394 Size: 441 475 57 62 183 798 3507 Fragments arranged by size: 3507 798 716 485 475 441 429 282 273 213 210 183 180 62 57 BsrDI GCAATG_nn' Cuts at: 65 1248 6806 6980 65 Size: 1183 5558 174 1396 Fragments arranged by size: 5558 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 2718 4144 6955 7142 1700 Size: 110 251 657 1426 2811 187 2869 Fragments arranged by size: 2869 2811 1426 657 251 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8311 BssHII G'CGCG_C Cuts at: 1925 2758 1925 Size: 833 7478 BssSI C'ACGA_G Cuts at: 2216 2402 4131 4793 4883 6371 7383 2216 Size: 186 1729 662 90 1488 1012 3144 Fragments arranged by size: 3144 1729 1488 1012 662 186 90 BstAPI GCAn_nnn'nTGC Cuts at: 794 3103 3115 3978 794 Size: 2309 12 863 5127 Fragments arranged by size: 5127 2309 863 12 BstBI TT'CG_AA Cuts at: 2007 7115 2007 Size: 5108 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8311 BstXI CCAn_nnnn'nTGG Cuts at: 2695 5558 2695 Size: 2863 5448 BstYI r'GATC_y Cuts at: 1972 3613 4656 4857 5124 5286 5641 6008 6393 Size: 1641 1043 201 267 162 355 367 385 Cuts at: 6393 6410 7862 7948 7960 8306 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1641 1452 1043 385 367 355 346 267 201 162 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4110 5350 4110 Size: 1240 7071 BtgI C'CryG_G Cuts at: 513 1065 2424 4253 4548 513 Size: 552 1359 1829 295 4276 Fragments arranged by size: 4276 1829 1359 552 295 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2233 487 Size: 683 56 1007 6565 Fragments arranged by size: 6565 1007 683 56 BtsI GCAGTG_nn' Cuts at: 2411 2574 3562 3637 4217 5231 5827 6621 6641 Size: 163 988 75 580 1014 596 794 20 Cuts at: 6641 2411 Size: 4081 Fragments arranged by size: 4081 1014 988 794 596 580 163 75 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8311 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8276 DraI TTT'AAA Cuts at: 5615 5615 Size: 8311 DraIII CAC_nnn'GTG Cuts at: 5035 5035 Size: 8311 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7318 809 Size: 507 6002 1802 Fragments arranged by size: 6002 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 2212 3072 Size: 54 1425 50 275 18 229 153 860 Cuts at: 3072 3474 3828 4039 4146 4416 4596 4635 4815 Size: 402 354 211 107 270 180 39 180 Cuts at: 4815 5758 6647 8276 8 Size: 943 889 1629 43 Fragments arranged by size: 1629 1425 943 889 860 402 354 275 270 229 211 180 180 153 107 54 50 43 39 18 EagI C'GGCC_G Cuts at: 1537 1812 5758 1537 Size: 275 3946 4090 Fragments arranged by size: 4090 3946 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 2504 2646 3048 3053 3237 4298 4387 Size: 966 185 142 402 5 184 1061 89 Cuts at: 4387 6241 1353 Size: 1854 3423 Fragments arranged by size: 3423 1854 1061 966 402 185 184 142 89 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2360 5428 6898 7272 7418 387 Size: 1064 138 771 3068 1470 374 146 1280 Fragments arranged by size: 3068 1470 1280 1064 771 374 146 138 EcoICRI GAG'CTC Cuts at: 2209 3568 4127 4996 2209 Size: 1359 559 869 5524 Fragments arranged by size: 5524 1359 869 559 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2171 2333 2360 2402 2576 2756 Size: 393 494 45 162 27 42 174 180 Cuts at: 2756 2768 2840 2966 3029 3248 3410 3422 3523 Size: 12 72 126 63 219 162 12 101 Cuts at: 3523 3584 3824 3872 3944 4143 4517 4583 5114 Size: 61 240 48 72 199 374 66 531 Cuts at: 5114 5207 5342 6374 6971 7758 8026 1239 Size: 93 135 1032 597 787 268 1524 Fragments arranged by size: 1524 1032 787 597 531 494 393 374 268 240 219 199 180 174 162 162 135 126 101 93 72 72 66 63 61 48 45 42 27 12 12 EcoNI CCTnn'n_nnAGG Cuts at: 2897 3410 3538 4499 2897 Size: 513 128 961 6709 Fragments arranged by size: 6709 961 513 128 EcoO109I rG'GnC_Cy Cuts at: 2515 2699 2743 4230 4247 4361 4516 5089 5434 Size: 184 44 1487 17 114 155 573 345 Cuts at: 5434 2515 Size: 5392 Fragments arranged by size: 5392 1487 573 345 184 155 114 44 17 EcoRI G'AATT_C Cuts at: 5330 5620 5998 5330 Size: 290 378 7643 Fragments arranged by size: 7643 378 290 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2515 2515 Size: 8311 FseI GG_CCGG'CC Cuts at: 4148 4148 Size: 8311 FspI TGC'GCA Cuts at: 2417 6817 2417 Size: 4400 3911 HaeII r_GCGC'y Cuts at: 1678 2078 2113 2146 2239 2437 2555 2788 2906 Size: 400 35 33 93 198 118 233 118 Cuts at: 2906 2956 2967 3176 3355 3389 5385 7458 1678 Size: 50 11 209 179 34 1996 2073 2531 Fragments arranged by size: 2531 2073 1996 400 233 209 198 179 118 118 93 50 35 34 33 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2966 2998 3779 3811 4790 Size: 32 135 32 1394 32 781 32 979 Cuts at: 4790 4822 6952 6984 7026 7058 1373 Size: 32 2130 32 42 32 2626 Fragments arranged by size: 2626 2130 1394 979 781 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5655 5901 669 Size: 988 281 3717 246 3079 Fragments arranged by size: 3717 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3434 748 Size: 2686 5625 HpaI GTT'AAC Cuts at: 5901 5901 Size: 8311 KpnI G_GTAC'C Cuts at: 5636 5636 Size: 8311 MfeI C'AATT_G Cuts at: 5910 5910 Size: 8311 MluI A'CGCG_T Cuts at: 8187 8187 Size: 8311 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2739 3063 3369 5289 7425 7609 1305 Size: 1434 324 306 1920 2136 184 2007 Fragments arranged by size: 2136 2007 1920 1434 324 306 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3830 4041 4418 4637 10 Size: 54 1425 343 1998 211 377 219 3684 Fragments arranged by size: 3684 1998 1425 377 343 219 211 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 4550 6269 6628 6787 518 Size: 648 1257 2127 1719 359 159 2042 Fragments arranged by size: 2127 2042 1719 1257 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 2308 2626 2776 2818 2914 2971 3070 Size: 588 114 318 150 42 96 57 99 Cuts at: 3070 3154 3226 3238 3265 3277 3511 3657 3850 Size: 84 72 12 27 12 234 146 193 Cuts at: 3850 3978 4687 4848 5462 5498 5727 6404 7552 Size: 128 709 161 614 36 229 677 1148 Cuts at: 7552 7797 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 709 677 614 588 318 245 234 229 193 161 150 146 128 114 99 96 84 72 57 42 36 27 12 12 NaeI GCC'GGC Cuts at: 2063 4146 2063 Size: 2083 6228 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7759 NdeI CA'TA_TG Cuts at: 387 4867 387 Size: 4480 3831 NgoMIV G'CCGG_C Cuts at: 2061 4144 2061 Size: 2083 6228 NheI G'CTAG_C Cuts at: 1052 3023 4435 1052 Size: 1971 1412 4928 Fragments arranged by size: 4928 1971 1412 NotI GC'GGCC_GC Cuts at: 5758 5758 Size: 8311 NspI r_CATG'y Cuts at: 1132 1229 4582 5669 7214 1132 Size: 97 3353 1087 1545 2229 Fragments arranged by size: 3353 2229 1545 1087 97 PasI CC'CwG_GG Cuts at: 1348 3734 5506 1348 Size: 2386 1772 4153 Fragments arranged by size: 4153 2386 1772 PciI A'CATG_T Cuts at: 7210 7210 Size: 8311 PflMI CCAn_nnn'nTGG Cuts at: 1108 3690 3734 4213 4723 1108 Size: 2582 44 479 510 4696 Fragments arranged by size: 4696 2582 510 479 44 PfoI T'CCnGG_A Cuts at: 2370 2532 4494 4716 5424 2370 Size: 162 1962 222 708 5257 Fragments arranged by size: 5257 1962 708 222 162 PmeI GTTT'AAAC Cuts at: 5615 5615 Size: 8311 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6380 6412 7893 7925 6380 Size: 32 1481 32 6766 Fragments arranged by size: 6766 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2699 4230 4247 4361 5089 5434 2699 Size: 1531 17 114 728 345 5576 Fragments arranged by size: 5576 1531 728 345 114 17 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8311 PsiI TTA'TAA Cuts at: 5881 5881 Size: 8311 PspOMI G'GGCC_C Cuts at: 2080 3267 4541 2080 Size: 1187 1274 5850 Fragments arranged by size: 5850 1274 1187 PspXI vC'TCGA_Gb Cuts at: 2573 2573 Size: 8311 PstI C_TGCA'G Cuts at: 830 2235 2886 3108 3120 3264 4224 5286 5497 Size: 1405 651 222 12 144 960 1062 211 Cuts at: 5497 5541 5663 830 Size: 44 122 3478 Fragments arranged by size: 3478 1405 1062 960 651 222 211 144 122 44 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8311 PvuII CAG'CTG Cuts at: 2194 2308 2626 2776 2818 3070 3154 3226 3238 Size: 114 318 150 42 252 84 72 12 Cuts at: 3238 3511 3850 3978 4848 5462 2194 Size: 273 339 128 870 614 5043 Fragments arranged by size: 5043 870 614 339 318 273 252 150 128 114 84 72 42 12 RsrII CG'GwC_CG Cuts at: 5587 5587 Size: 8311 SacI G_AGCT'C Cuts at: 2211 3570 4129 4998 2211 Size: 1359 559 869 5524 Fragments arranged by size: 5524 1359 869 559 SalI G'TCGA_C Cuts at: 1936 5653 1936 Size: 3717 4594 SanDI GG'GwC_CC Cuts at: 4230 4247 4361 4230 Size: 17 114 8180 Fragments arranged by size: 8180 114 17 SapI GCTCTTCn'nnn_ Cuts at: 2646 3053 4298 4387 2646 Size: 407 1245 89 6570 Fragments arranged by size: 6570 1245 407 89 SbfI CC_TGCA'GG Cuts at: 5663 5663 Size: 8311 ScaI AGT'ACT Cuts at: 6559 8271 6559 Size: 1712 6599 SfcI C'TryA_G Cuts at: 826 1045 2184 2231 2882 3104 3116 3260 4220 Size: 219 1139 47 651 222 12 144 960 Cuts at: 4220 5282 5493 5537 5659 6794 7475 7666 826 Size: 1062 211 44 122 1135 681 191 1471 Fragments arranged by size: 1471 1139 1135 1062 960 681 651 222 219 211 191 144 122 47 44 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8311 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8311 SmaI CCC'GGG Cuts at: 1872 2451 5570 5638 1872 Size: 579 3119 68 4545 Fragments arranged by size: 4545 3119 579 68 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 2894 3368 4193 4697 5009 Size: 197 926 630 321 474 825 504 312 Cuts at: 5009 6415 7316 7578 820 Size: 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 825 630 504 474 321 312 262 197 SnaBI TAC'GTA Cuts at: 493 5608 6102 493 Size: 5115 494 2702 Fragments arranged by size: 5115 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8311 SphI G_CATG'C Cuts at: 1132 4582 5669 1132 Size: 3450 1087 3774 Fragments arranged by size: 3774 3450 1087 SspI AAT'ATT Cuts at: 5 52 6235 5 Size: 47 6183 2081 Fragments arranged by size: 6183 2081 47 StuI AGG'CCT Cuts at: 1517 4211 4505 1517 Size: 2694 294 5323 Fragments arranged by size: 5323 2694 294 StyI C'CwwG_G Cuts at: 513 1065 3684 4212 4836 5750 513 Size: 552 2619 528 624 914 3074 Fragments arranged by size: 3074 2619 914 624 552 528 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3601 4186 4482 4561 4879 5158 5575 Size: 1255 2021 585 296 79 318 279 417 Cuts at: 5575 6338 6355 6508 6693 7138 325 Size: 763 17 153 185 445 1498 Fragments arranged by size: 2021 1498 1255 763 585 445 417 318 296 279 185 153 79 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6557 8269 96 Size: 274 80 33 51 1443 4580 1712 138 Fragments arranged by size: 4580 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3714 3746 8271 8303 3714 Size: 32 4525 32 3722 Fragments arranged by size: 4525 3722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8311 XbaI T'CTAG_A Cuts at: 5647 5647 Size: 8311 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2450 3004 3946 4426 4720 2450 Size: 554 942 480 294 6041 Fragments arranged by size: 6041 942 554 480 294 XhoI C'TCGA_G Cuts at: 1943 2573 1943 Size: 630 7681 XmaI C'CCGG_G Cuts at: 1870 2449 5568 5636 1870 Size: 579 3119 68 4545 Fragments arranged by size: 4545 3119 579 68 XmnI GAAnn'nnTTC Cuts at: 5330 5420 6440 5330 Size: 90 1020 7201 Fragments arranged by size: 7201 1020 90 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6119 276 Size: 53 83 186 936 4585 2468 Fragments arranged by size: 4585 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FspAI KasI NarI NruI NsiI PacI PmlI PsrI SacII SexAI SfiI SfoI SrfI SwaI