Restriction map for
pFN21ASDA0654
(Linear) (Six-base) MAPSORT of: KIAA0654.seq Check: 6492 from: 1 to: 3582
KIAA0654.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 3018 3582
Size: 3018 564
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1399 1801 3184 3582
Size: 337 1062 402 1383 398
Fragments arranged by size:
1383 1062 402 398 337
AfeI AGC'GCT
Cuts at: 0 931 1330 3582
Size: 931 399 2252
Fragments arranged by size:
2252 931 399
AflIII A'CryG_T
Cuts at: 0 2756 3582
Size: 2756 826
AleI CACnn'nnGTG
Cuts at: 0 2527 3582
Size: 2527 1055
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 247 677 2680 3582
Size: 247 430 2003 902
Fragments arranged by size:
2003 902 430 247
AlwNI CAG_nnn'CTG
Cuts at: 0 288 1080 1092 1803 1955 2838 3297 3582
Size: 288 792 12 711 152 883 459 285
Fragments arranged by size:
883 792 711 459 288 285 152 12
ApaI G_GGCC'C
Cuts at: 0 61 1248 2522 3582
Size: 61 1187 1274 1060
Fragments arranged by size:
1274 1187 1060 61
ApoI r'AATT_y
Cuts at: 0 1179 3169 3307 3582
Size: 1179 1990 138 275
Fragments arranged by size:
1990 1179 275 138
AvaI C'yCGr_G
Cuts at: 0 426 550 1114 2806 3545 3582
Size: 426 124 564 1692 739 37
Fragments arranged by size:
1692 739 564 426 124 37
BamHI G'GATC_C
Cuts at: 0 1590 3582
Size: 1590 1992
BanI G'GyrC_C
Cuts at: 0 811 1560 1579 2037 2062 2245 2602 3582
Size: 811 749 19 458 25 183 357 980
Fragments arranged by size:
980 811 749 458 357 183 25 19
BanII G_rGCy'C
Cuts at: 0 49 61 188 1248 1547 1658 1693 2106
Size: 49 12 127 1060 299 111 35 413
Cuts at: 2106 2522 2975 3483 3490 3500 3582
Size: 416 453 508 7 10 82
Fragments arranged by size:
1060 508 453 416 413 299 127 111
82 49 35 12 10 7
BbvCI CC'TCA_GC
Cuts at: 0 2721 3582
Size: 2721 861
BglI GCCn_nnn'nGGC
Cuts at: 0 44 2492 2584 2611 3582
Size: 44 2448 92 27 971
Fragments arranged by size:
2448 971 92 44 27
BlpI GC'TnA_GC
Cuts at: 0 220 1312 3582
Size: 220 1092 2270
Fragments arranged by size:
2270 1092 220
Bme1580I G_kGCm'C
Cuts at: 0 61 814 1248 1539 1584 2065 2248 2522
Size: 61 753 434 291 45 481 183 274
Cuts at: 2522 3210 3582
Size: 688 372
Fragments arranged by size:
753 688 481 434 372 291 274 183
61 45
BmgBI CAC'GTC
Cuts at: 0 959 1794 3111 3582
Size: 959 835 1317 471
Fragments arranged by size:
1317 959 835 471
BmrI ACTGGGnnnn_n'
Cuts at: 0 414 1772 3582
Size: 414 1358 1810
Fragments arranged by size:
1810 1358 414
BmtI G_CTAG'C
Cuts at: 0 1004 2416 3582
Size: 1004 1412 1166
Fragments arranged by size:
1412 1166 1004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 1395 1674 2721 3582
Size: 219 1176 279 1047 861
Fragments arranged by size:
1176 1047 861 279 219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 148 310 379 553 733 745 817
Size: 103 45 162 69 174 180 12 72
Cuts at: 817 943 1006 1225 1387 1500 1561 1849 1921
Size: 126 63 219 162 113 61 288 72
Cuts at: 1921 2120 2494 2560 3091 3319 3582
Size: 199 374 66 531 228 263
Fragments arranged by size:
531 374 288 263 228 219 199 180
174 162 162 126 113 103 72 72
69 66 63 61 45 12
Bpu10I CC'TnA_GC
Cuts at: 0 937 1384 2721 3582
Size: 937 447 1337 861
Fragments arranged by size:
1337 937 861 447
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 1330 2191 2659 2971 3582
Size: 856 474 861 468 312 611
Fragments arranged by size:
861 856 611 474 468 312
BsaI GGTCTCn'nnnn_
Cuts at: 0 441 1099 1526 2709 2803 3220 3582
Size: 441 658 427 1183 94 417 362
Fragments arranged by size:
1183 658 441 427 417 362 94
BsaBI GATnn'nnATC
Cuts at: 0 1477 2650 3582
Size: 1477 1173 932
Fragments arranged by size:
1477 1173 932
BsaHI Gr'CG_yC
Cuts at: 0 971 1067 3582
Size: 971 96 2515
Fragments arranged by size:
2515 971 96
BsaWI w'CCGG_w
Cuts at: 0 3059 3566 3582
Size: 3059 507 16
Fragments arranged by size:
3059 507 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 943 973 979 1000 1009 1030
Size: 790 30 123 30 6 21 9 21
Cuts at: 1030 2767 2797 3307 3337 3582
Size: 1737 30 510 30 245
Fragments arranged by size:
1737 790 510 245 123 30 30 30
30 21 21 9 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 429 568 571 730 814 817 919 1201
Size: 429 139 3 159 84 3 102 282
Cuts at: 1201 1384 1405 1666 1750 1753 1879 1909 2491
Size: 183 21 261 84 3 126 30 582
Cuts at: 2491 2724 2788 3582
Size: 233 64 794
Fragments arranged by size:
794 582 429 282 261 233 183 159
139 126 102 84 84 64 30 21
3 3 3
BseYI C'CCAG_C
Cuts at: 0 54 828 944 1211 2008 2825 2887 3156
Size: 54 774 116 267 797 817 62 269
Cuts at: 3156 3582
Size: 426
Fragments arranged by size:
817 797 774 426 269 267 116 62
54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 949 1816 2245 2650 3582
Size: 949 867 429 405 932
Fragments arranged by size:
949 932 867 429 405
BsiHKAI G_wGCw'C
Cuts at: 0 188 458 485 551 1547 2106 2975 3582
Size: 188 270 27 66 996 559 869 607
Fragments arranged by size:
996 869 607 559 270 188 66 27
BsmI GAATG_Cn'
Cuts at: 0 3282 3582
Size: 3282 300
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 965 1288 2311 3101 3582
Size: 124 841 323 1023 790 481
Fragments arranged by size:
1023 841 790 481 323 124
Bsp1286I G_dGCh'C
Cuts at: 0 49 61 188 458 485 551 814 1248
Size: 49 12 127 270 27 66 263 434
Cuts at: 1248 1539 1547 1584 1658 1693 2065 2106 2248
Size: 291 8 37 74 35 372 41 142
Cuts at: 2248 2522 2975 3210 3483 3490 3500 3582
Size: 274 453 235 273 7 10 82
Fragments arranged by size:
453 434 372 291 274 273 270 263
235 142 127 82 74 66 49 41
37 35 27 12 10 8 7
BspEI T'CCGG_A
Cuts at: 0 3566 3582
Size: 3566 16
BsrBI CCG'CTC
Cuts at: 0 516 729 909 1191 1464 1674 2159 2600
Size: 516 213 180 282 273 210 485 441
Cuts at: 2600 3075 3132 3194 3377 3582
Size: 475 57 62 183 205
Fragments arranged by size:
516 485 475 441 282 273 213 210
205 183 180 62 57
BsrFI r'CCGG_y
Cuts at: 0 38 695 2121 3582
Size: 38 657 1426 1461
Fragments arranged by size:
1461 1426 657 38
BssHII G'CGCG_C
Cuts at: 0 735 3582
Size: 735 2847
BssSI C'ACGA_G
Cuts at: 0 193 379 2108 2770 2860 3582
Size: 193 186 1729 662 90 722
Fragments arranged by size:
1729 722 662 193 186 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1080 1092 1955 3582
Size: 1080 12 863 1627
Fragments arranged by size:
1627 1080 863 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 3535 3582
Size: 672 2863 47
Fragments arranged by size:
2863 672 47
BstYI r'GATC_y
Cuts at: 0 1590 2633 2834 3101 3263 3582
Size: 1590 1043 201 267 162 319
Fragments arranged by size:
1590 1043 319 267 201 162
Bsu36I CC'TnA_GG
Cuts at: 0 2087 3327 3582
Size: 2087 1240 255
Fragments arranged by size:
2087 1240 255
BtgI C'CryG_G
Cuts at: 0 401 2230 2525 3582
Size: 401 1829 295 1057
Fragments arranged by size:
1829 1057 401 295
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 210 3582
Size: 210 3372
BtsI GCAGTG_nn'
Cuts at: 0 388 551 1539 1614 2194 3208 3582
Size: 388 163 988 75 580 1014 374
Fragments arranged by size:
1014 988 580 388 374 163 75
DraIII CAC_nnn'GTG
Cuts at: 0 3012 3582
Size: 3012 570
EaeI y'GGCC_r
Cuts at: 0 36 189 1049 1451 1805 2016 2123 2393
Size: 36 153 860 402 354 211 107 270
Cuts at: 2393 2573 2612 2792 3582
Size: 180 39 180 790
Fragments arranged by size:
860 790 402 354 270 211 180 180
153 107 39 36
EarI CTCTTCn'nnn_
Cuts at: 0 296 481 623 1025 1030 1214 2275 2364
Size: 296 185 142 402 5 184 1061 89
Cuts at: 2364 3582
Size: 1218
Fragments arranged by size:
1218 1061 402 296 185 184 142 89
5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 337 3405 3582
Size: 337 3068 177
Fragments arranged by size:
3068 337 177
EcoICRI GAG'CTC
Cuts at: 0 186 1545 2104 2973 3582
Size: 186 1359 559 869 609
Fragments arranged by size:
1359 869 609 559 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 148 310 337 379 553 733 745
Size: 103 45 162 27 42 174 180 12
Cuts at: 745 817 943 1006 1225 1387 1399 1500 1561
Size: 72 126 63 219 162 12 101 61
Cuts at: 1561 1801 1849 1921 2120 2494 2560 3091 3184
Size: 240 48 72 199 374 66 531 93
Cuts at: 3184 3319 3582
Size: 135 263
Fragments arranged by size:
531 374 263 240 219 199 180 174
162 162 135 126 103 101 93 72
72 66 63 61 48 45 42 27
12 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 874 1387 1515 2476 3582
Size: 874 513 128 961 1106
Fragments arranged by size:
1106 961 874 513 128
EcoO109I rG'GnC_Cy
Cuts at: 0 492 676 720 2207 2224 2338 2493 3066
Size: 492 184 44 1487 17 114 155 573
Cuts at: 3066 3411 3582
Size: 345 171
Fragments arranged by size:
1487 573 492 345 184 171 155 114
44 17
EcoRI G'AATT_C
Cuts at: 0 3307 3582
Size: 3307 275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 492 3582
Size: 492 3090
FseI GG_CCGG'CC
Cuts at: 0 2125 3582
Size: 2125 1457
FspI TGC'GCA
Cuts at: 0 394 3582
Size: 394 3188
HaeII r_GCGC'y
Cuts at: 0 55 90 123 216 414 532 765 883
Size: 55 35 33 93 198 118 233 118
Cuts at: 883 933 944 1153 1332 1366 3362 3582
Size: 50 11 209 179 34 1996 220
Fragments arranged by size:
1996 233 220 209 198 179 118 118
93 55 50 35 34 33 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 943 975 1756 1788 2767 2799 3582
Size: 943 32 781 32 979 32 783
Fragments arranged by size:
979 943 783 781 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1411 3582
Size: 1411 2171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1040 1346 3266 3582
Size: 716 324 306 1920 316
Fragments arranged by size:
1920 716 324 316 306
MscI TGG'CCA
Cuts at: 0 1807 2018 2395 2614 3582
Size: 1807 211 377 219 968
Fragments arranged by size:
1807 968 377 219 211
MslI CAynn'nnrTG
Cuts at: 0 400 2527 3582
Size: 400 2127 1055
Fragments arranged by size:
2127 1055 400
MspA1I CmG'CkG
Cuts at: 0 171 285 603 753 795 891 948 1047
Size: 171 114 318 150 42 96 57 99
Cuts at: 1047 1131 1203 1215 1242 1254 1488 1634 1827
Size: 84 72 12 27 12 234 146 193
Cuts at: 1827 1955 2664 2825 3439 3475 3582
Size: 128 709 161 614 36 107
Fragments arranged by size:
709 614 318 234 193 171 161 150
146 128 114 107 99 96 84 72
57 42 36 27 12 12
NaeI GCC'GGC
Cuts at: 0 40 2123 3582
Size: 40 2083 1459
Fragments arranged by size:
2083 1459 40
NdeI CA'TA_TG
Cuts at: 0 2844 3582
Size: 2844 738
NgoMIV G'CCGG_C
Cuts at: 0 38 2121 3582
Size: 38 2083 1461
Fragments arranged by size:
2083 1461 38
NheI G'CTAG_C
Cuts at: 0 1000 2412 3582
Size: 1000 1412 1170
Fragments arranged by size:
1412 1170 1000
NspI r_CATG'y
Cuts at: 0 2559 3582
Size: 2559 1023
PasI CC'CwG_GG
Cuts at: 0 1711 3483 3582
Size: 1711 1772 99
Fragments arranged by size:
1772 1711 99
PflMI CCAn_nnn'nTGG
Cuts at: 0 1667 1711 2190 2700 3582
Size: 1667 44 479 510 882
Fragments arranged by size:
1667 882 510 479 44
PfoI T'CCnGG_A
Cuts at: 0 347 509 2471 2693 3401 3582
Size: 347 162 1962 222 708 181
Fragments arranged by size:
1962 708 347 222 181 162
PpuMI rG'GwC_Cy
Cuts at: 0 676 2207 2224 2338 3066 3411 3582
Size: 676 1531 17 114 728 345 171
Fragments arranged by size:
1531 728 676 345 171 114 17
PspOMI G'GGCC_C
Cuts at: 0 57 1244 2518 3582
Size: 57 1187 1274 1064
Fragments arranged by size:
1274 1187 1064 57
PspXI vC'TCGA_Gb
Cuts at: 0 550 3582
Size: 550 3032
PstI C_TGCA'G
Cuts at: 0 212 863 1085 1097 1241 2201 3263 3474
Size: 212 651 222 12 144 960 1062 211
Cuts at: 3474 3518 3582
Size: 44 64
Fragments arranged by size:
1062 960 651 222 212 211 144 64
44 12
PvuII CAG'CTG
Cuts at: 0 171 285 603 753 795 1047 1131 1203
Size: 171 114 318 150 42 252 84 72
Cuts at: 1203 1215 1488 1827 1955 2825 3439 3582
Size: 12 273 339 128 870 614 143
Fragments arranged by size:
870 614 339 318 273 252 171 150
143 128 114 84 72 42 12
RsrII CG'GwC_CG
Cuts at: 0 3564 3582
Size: 3564 18
SacI G_AGCT'C
Cuts at: 0 188 1547 2106 2975 3582
Size: 188 1359 559 869 607
Fragments arranged by size:
1359 869 607 559 188
SanDI GG'GwC_CC
Cuts at: 0 2207 2224 2338 3582
Size: 2207 17 114 1244
Fragments arranged by size:
2207 1244 114 17
SapI GCTCTTCn'nnn_
Cuts at: 0 623 1030 2275 2364 3582
Size: 623 407 1245 89 1218
Fragments arranged by size:
1245 1218 623 407 89
SfcI C'TryA_G
Cuts at: 0 161 208 859 1081 1093 1237 2197 3259
Size: 161 47 651 222 12 144 960 1062
Cuts at: 3259 3470 3514 3582
Size: 211 44 68
Fragments arranged by size:
1062 960 651 222 211 161 144 68
47 44 12
SmaI CCC'GGG
Cuts at: 0 428 3547 3582
Size: 428 3119 35
Fragments arranged by size:
3119 428 35
SmlI C'TyrA_G
Cuts at: 0 550 871 1345 2170 2674 2986 3582
Size: 550 321 474 825 504 312 596
Fragments arranged by size:
825 596 550 504 474 321 312
SphI G_CATG'C
Cuts at: 0 2559 3582
Size: 2559 1023
StuI AGG'CCT
Cuts at: 0 2188 2482 3582
Size: 2188 294 1100
Fragments arranged by size:
2188 1100 294
StyI C'CwwG_G
Cuts at: 0 1661 2189 2813 3582
Size: 1661 528 624 769
Fragments arranged by size:
1661 769 624 528
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1578 2163 2459 2538 2856 3135 3552 3582
Size: 1578 585 296 79 318 279 417 30
Fragments arranged by size:
1578 585 417 318 296 279 79 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1691 1723 3582
Size: 1691 32 1859
Fragments arranged by size:
1859 1691 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 427 981 1923 2403 2697 3582
Size: 427 554 942 480 294 885
Fragments arranged by size:
942 885 554 480 427 294
XhoI C'TCGA_G
Cuts at: 0 550 3582
Size: 550 3032
XmaI C'CCGG_G
Cuts at: 0 426 3545 3582
Size: 426 3119 37
Fragments arranged by size:
3119 426 37
XmnI GAAnn'nnTTC
Cuts at: 0 3307 3397 3582
Size: 3307 90 185
Fragments arranged by size:
3307 185 90
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbvCI BglI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV NheI NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII
RsrII SacI SanDI SapI SfcI SmaI SmlI SphI
StuI StyI TaqII TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BcgI BciVI BclI BfrBI BglII BsaAI BsiEI BsiWI
BspHI BspMI BsrDI BsrGI BstBI BstEII BstZ17I ClaI
CspCI DraI DrdI EagI EcoRV FspAI HincII HpaI
KasI KpnI MfeI MluI NarI NcoI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PvuI SacII SalI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TatI Tth111I XbaI ZraI