(Circular) (Six-base) MAPSORT of: pFN21ASDA0649.seq Check: 9826 from: 1 to: 8356 pFN21ASDA0649 8356 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6166 278 Size: 53 83 186 936 4630 2468 Fragments arranged by size: 4630 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5649 5699 1937 Size: 3712 50 4594 Fragments arranged by size: 4594 3712 50 Acc65I G'GTAC_C Cuts at: 5677 5677 Size: 8356 AclI AA'CG_TT Cuts at: 1563 6483 6856 1563 Size: 4920 373 3063 Fragments arranged by size: 4920 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 2988 3392 4083 4292 5314 5362 5584 Size: 833 523 404 691 209 1022 48 222 Cuts at: 5584 6419 7803 8071 1632 Size: 835 1384 268 1917 Fragments arranged by size: 1917 1384 1022 835 833 691 523 404 268 222 209 48 AfeI AGC'GCT Cuts at: 3049 3049 Size: 8356 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8159 AflIII A'CryG_T Cuts at: 1203 7255 8232 1203 Size: 6052 977 1327 Fragments arranged by size: 6052 1327 977 AgeI A'CCGG_T Cuts at: 7187 7187 Size: 8356 AhdI GACnn_n'nnGTC Cuts at: 5206 5546 7085 5206 Size: 340 1539 6477 Fragments arranged by size: 6477 1539 340 AleI CACnn'nnGTG Cuts at: 1166 2559 1166 Size: 1393 6963 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2269 3010 4389 1184 Size: 1085 741 1379 5151 Fragments arranged by size: 5151 1379 1085 741 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3560 3592 3560 Size: 32 8324 AlwNI CAG_nnn'CTG Cuts at: 2722 2860 6099 7671 2722 Size: 138 3239 1572 3407 Fragments arranged by size: 3407 3239 1572 138 ApaI G_GGCC'C Cuts at: 2488 3989 4262 4472 4610 4634 5219 5363 2488 Size: 1501 273 210 138 24 585 144 5481 Fragments arranged by size: 5481 1501 585 273 210 144 138 24 ApaLI G'TGCA_C Cuts at: 2288 4687 6413 7569 2288 Size: 2399 1726 1156 3075 Fragments arranged by size: 3075 2399 1726 1156 ApoI r'AATT_y Cuts at: 1493 2794 5665 5891 6043 8152 1493 Size: 1301 2871 226 152 2109 1697 Fragments arranged by size: 2871 2109 1697 1301 226 152 AseI AT'TA_AT Cuts at: 160 1032 6910 160 Size: 872 5878 1606 Fragments arranged by size: 5878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8356 AvaI C'yCGr_G Cuts at: 1870 1943 2157 2202 2471 3269 4818 5032 5053 Size: 73 214 45 269 798 1549 214 21 Cuts at: 5053 5223 5503 5681 1870 Size: 170 280 178 4545 Fragments arranged by size: 4545 1549 798 280 269 214 214 178 170 73 45 21 BamHI G'GATC_C Cuts at: 2630 4825 5686 6053 2630 Size: 2195 861 367 4933 Fragments arranged by size: 4933 2195 861 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 3974 4038 4373 4831 Size: 325 217 627 420 1767 64 335 458 Cuts at: 4831 4982 4997 5677 7132 618 Size: 151 15 680 1455 1842 Fragments arranged by size: 1842 1767 1455 680 627 458 420 335 325 217 151 64 15 BanII G_rGCy'C Cuts at: 1391 2488 2651 3404 3912 3989 4061 4164 4236 Size: 1097 163 753 508 77 72 103 72 Cuts at: 4236 4262 4472 4610 4634 4803 4875 4919 5033 Size: 26 210 138 24 169 72 44 114 Cuts at: 5033 5080 5219 5363 5504 5510 5675 1391 Size: 47 139 144 141 6 165 4072 Fragments arranged by size: 4072 1097 753 508 210 169 165 163 144 141 139 138 114 103 77 72 72 72 47 44 26 24 6 BbeI G_GCGC'C Cuts at: 4042 5001 4042 Size: 959 7397 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4071 928 Size: 971 2172 5213 Fragments arranged by size: 5213 2172 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2180 2214 6547 6581 1903 Size: 34 243 34 4333 34 3678 Fragments arranged by size: 4333 3678 243 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6248 7458 6248 Size: 1210 7146 BclI T'GATC_A Cuts at: 1548 1548 Size: 8356 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2270 2483 2865 Size: 107 122 71 631 605 598 213 382 Cuts at: 2865 3656 3747 3956 6967 136 Size: 791 91 209 3011 1525 Fragments arranged by size: 3011 1525 791 631 605 598 382 213 209 122 107 91 71 BglII A'GATC_T Cuts at: 8351 8351 Size: 8356 BlpI GC'TnA_GC Cuts at: 3459 3906 4929 5773 3459 Size: 447 1023 844 6042 Fragments arranged by size: 6042 1023 844 447 Bme1580I G_kGCm'C Cuts at: 1790 2210 2292 2488 2566 3977 3989 4262 4472 Size: 420 82 196 78 1411 12 273 210 Cuts at: 4472 4610 4634 4691 4769 4985 5219 5363 6417 Size: 138 24 57 78 216 234 144 1054 Cuts at: 6417 7573 1790 Size: 1156 2573 Fragments arranged by size: 2573 1411 1156 1054 420 273 234 216 210 196 144 138 82 78 78 57 24 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4326 6087 7045 Size: 416 53 83 399 24 2909 1761 958 Cuts at: 7045 442 Size: 1753 Fragments arranged by size: 2909 1761 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2028 2042 3091 3378 4559 5094 5122 7016 Size: 789 14 1049 287 1181 535 28 1894 Cuts at: 7016 1239 Size: 2579 Fragments arranged by size: 2579 1894 1181 1049 789 535 287 28 14 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4127 6481 7346 7644 4127 Size: 2354 865 298 4839 Fragments arranged by size: 4839 2354 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2327 2738 3266 3921 4408 7019 882 Size: 617 828 411 528 655 487 2611 2219 Fragments arranged by size: 2611 2219 828 655 617 528 487 411 BsaAI yAC'GTr Cuts at: 493 1204 5653 6147 493 Size: 711 4449 494 2702 Fragments arranged by size: 4449 2702 711 494 BsaBI GATnn'nnATC Cuts at: 2171 8350 2171 Size: 6179 2177 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2262 2868 3089 3098 Size: 53 83 186 936 729 606 221 9 Cuts at: 3098 4039 4998 5184 5574 5589 6163 6545 7180 Size: 941 959 186 390 15 574 382 635 Cuts at: 7180 275 Size: 1451 Fragments arranged by size: 1451 959 941 936 729 635 606 574 390 382 221 186 186 83 53 15 9 BsaWI w'CCGG_w Cuts at: 3636 4236 4828 6789 7187 7461 7608 8019 3636 Size: 600 592 1961 398 274 147 411 3973 Fragments arranged by size: 3973 1961 600 592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2129 2159 2633 2663 3370 Size: 30 165 30 726 30 474 30 707 Cuts at: 3370 3400 3584 3614 1178 Size: 30 184 30 5920 Fragments arranged by size: 5920 726 707 474 184 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2366 2438 3143 3224 4426 5512 1186 Size: 1180 72 705 81 1202 1086 4030 Fragments arranged by size: 4030 1202 1180 1086 705 81 72 BseYI C'CCAG_C Cuts at: 2487 2502 2863 3290 3790 4225 4471 4478 4633 Size: 15 361 427 500 435 246 7 155 Cuts at: 4633 4964 5048 5132 5234 5274 5399 7559 8055 Size: 331 84 84 102 40 125 2160 496 Cuts at: 8055 2487 Size: 2788 Fragments arranged by size: 2788 2160 500 496 435 427 361 331 246 155 125 102 84 84 40 15 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2517 3492 4174 4195 4675 1163 Size: 573 447 334 975 682 21 480 4844 Fragments arranged by size: 4844 975 682 573 480 447 334 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5089 5806 6567 7595 1540 Size: 275 186 3088 717 761 1028 2301 Fragments arranged by size: 3088 2301 1028 761 717 275 186 BsiHKAI G_wGCw'C Cuts at: 2292 4061 4691 4762 4847 5504 6417 6502 7573 Size: 1769 630 71 85 657 913 85 1071 Cuts at: 7573 2292 Size: 3075 Fragments arranged by size: 3075 1769 1071 913 657 630 85 85 71 BsmI GAATG_Cn' Cuts at: 5867 5960 5867 Size: 93 8263 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2210 2292 2488 2566 2651 3404 3912 Size: 399 420 82 196 78 85 753 508 Cuts at: 3912 3977 3989 4061 4164 4236 4262 4472 4610 Size: 65 12 72 103 72 26 210 138 Cuts at: 4610 4634 4691 4762 4769 4803 4847 4875 4919 Size: 24 57 71 7 34 44 28 44 Cuts at: 4919 4985 5033 5080 5219 5363 5504 5510 5675 Size: 66 48 47 139 144 141 6 165 Cuts at: 5675 6417 6502 7573 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 753 742 508 420 399 210 196 165 144 141 139 138 103 85 85 82 78 72 72 71 66 65 57 48 47 44 44 34 28 26 24 12 7 6 BspEI T'CCGG_A Cuts at: 3636 4236 8019 3636 Size: 600 3783 3973 Fragments arranged by size: 3973 3783 600 BspHI T'CATG_A Cuts at: 2031 3072 6245 7975 2031 Size: 1041 3173 1730 2412 Fragments arranged by size: 3173 2412 1730 1041 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3192 3939 5711 844 Size: 2348 747 1772 3489 Fragments arranged by size: 3489 2348 1772 747 BsrBI CCG'CTC Cuts at: 1394 1823 3582 4616 5060 6243 1394 Size: 429 1759 1034 444 1183 3507 Fragments arranged by size: 3507 1759 1183 1034 444 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6851 7025 65 Size: 1183 5603 174 1396 Fragments arranged by size: 5603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2269 2477 2548 2983 3508 3746 4666 Size: 110 459 208 71 435 525 238 920 Cuts at: 4666 7000 7187 1700 Size: 2334 187 2869 Fragments arranged by size: 2869 2334 920 525 459 435 238 208 187 110 71 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8356 BssHII G'CGCG_C Cuts at: 1925 2221 1925 Size: 296 8060 BssSI C'ACGA_G Cuts at: 3436 4920 6416 7428 3436 Size: 1484 1496 1012 4364 Fragments arranged by size: 4364 1496 1484 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2860 794 Size: 2066 6290 BstBI TT'CG_AA Cuts at: 2007 7160 2007 Size: 5153 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8356 BstXI CCAn_nnnn'nTGG Cuts at: 3322 3322 Size: 8356 BstYI r'GATC_y Cuts at: 1972 2630 4825 5686 6053 6438 6455 7907 7993 Size: 658 2195 861 367 385 17 1452 86 Cuts at: 7993 8005 8351 1972 Size: 12 346 1977 Fragments arranged by size: 2195 1977 1452 861 658 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2936 5531 2936 Size: 2595 5761 BtgI C'CryG_G Cuts at: 513 1065 2343 2406 3321 3603 5138 5613 513 Size: 552 1278 63 915 282 1535 475 3256 Fragments arranged by size: 3256 1535 1278 915 552 475 282 63 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2229 2691 3627 487 Size: 683 56 1003 462 936 5216 Fragments arranged by size: 5216 1003 936 683 462 56 BtsI GCAGTG_nn' Cuts at: 2566 3253 3532 3785 5175 5590 5872 6666 6686 Size: 687 279 253 1390 415 282 794 20 Cuts at: 6686 2566 Size: 4236 Fragments arranged by size: 4236 1390 794 687 415 282 279 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2289 2324 4600 4635 529 Size: 35 1725 35 2276 35 4250 Fragments arranged by size: 4250 2276 1725 35 35 35 DraI TTT'AAA Cuts at: 5660 5660 Size: 8356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7363 809 Size: 507 6047 1802 Fragments arranged by size: 6047 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3309 3678 3751 Size: 54 1425 50 275 18 1479 369 73 Cuts at: 3751 3820 3913 4725 5453 5803 6692 8321 8 Size: 69 93 812 728 350 889 1629 43 Fragments arranged by size: 1629 1479 1425 889 812 728 369 350 275 93 73 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5803 1537 Size: 275 3991 4090 Fragments arranged by size: 4090 3991 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 3560 4142 4268 5285 5333 6286 1353 Size: 1373 834 582 126 1017 48 953 3423 Fragments arranged by size: 3423 1373 1017 953 834 582 126 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2538 2641 2720 2788 3461 4414 Size: 1064 138 949 103 79 68 673 953 Cuts at: 4414 4574 4852 6943 7317 7463 387 Size: 160 278 2091 374 146 1280 Fragments arranged by size: 2091 1280 1064 953 949 673 374 278 160 146 138 103 79 68 EcoICRI GAG'CTC Cuts at: 4059 5502 4059 Size: 1443 6913 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2028 2042 2465 2988 3091 3378 3392 Size: 393 396 14 423 523 103 287 14 Cuts at: 3392 4083 4292 4559 5094 5122 5314 5362 5584 Size: 691 209 267 535 28 192 48 222 Cuts at: 5584 6419 7016 7803 8071 1239 Size: 835 597 787 268 1524 Fragments arranged by size: 1524 835 787 691 597 535 523 423 396 393 287 268 267 222 209 192 103 48 28 14 14 EcoNI CCTnn'n_nnAGG Cuts at: 2601 3333 4818 2601 Size: 732 1485 6139 Fragments arranged by size: 6139 1485 732 EcoO109I rG'GnC_Cy Cuts at: 2484 2857 3181 3340 3534 3958 3985 3986 4096 Size: 373 324 159 194 424 27 1 110 Cuts at: 4096 4258 4313 4468 4606 4607 4630 4714 5215 Size: 162 55 155 138 1 23 84 501 Cuts at: 5215 5216 5227 5359 5360 2484 Size: 1 11 132 1 5480 Fragments arranged by size: 5480 501 424 373 324 194 162 159 155 138 132 110 84 55 27 23 11 1 1 1 1 EcoRI G'AATT_C Cuts at: 5665 6043 5665 Size: 378 7978 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 8356 FspI TGC'GCA Cuts at: 6862 6862 Size: 8356 HaeII r_GCGC'y Cuts at: 1678 2157 3045 3051 3221 4042 5001 7503 1678 Size: 479 888 6 170 821 959 2502 2531 Fragments arranged by size: 2531 2502 959 888 821 479 170 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2129 2161 2633 2665 3584 Size: 32 135 32 557 32 472 32 919 Cuts at: 3584 3616 5192 5224 5532 5564 6997 7029 7071 Size: 32 1576 32 308 32 1433 32 42 Cuts at: 7071 7103 1373 Size: 32 2626 Fragments arranged by size: 2626 1576 1433 919 557 472 308 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5551 5700 5946 669 Size: 988 281 3613 149 246 3079 Fragments arranged by size: 3613 3079 988 281 246 149 HindIII A'AGCT_T Cuts at: 748 748 Size: 8356 HpaI GTT'AAC Cuts at: 5946 5946 Size: 8356 KasI G'GCGC_C Cuts at: 4038 4997 4038 Size: 959 7397 KpnI G_GTAC'C Cuts at: 5681 5681 Size: 8356 MfeI C'AATT_G Cuts at: 5955 5955 Size: 8356 MluI A'CGCG_T Cuts at: 8232 8232 Size: 8356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3126 3223 7470 7654 1305 Size: 1821 97 4247 184 2007 Fragments arranged by size: 4247 2007 1821 184 97 MscI TGG'CCA Cuts at: 10 64 1489 1832 3311 3680 3822 5455 10 Size: 54 1425 343 1479 369 142 1633 2911 Fragments arranged by size: 2911 1633 1479 1425 369 343 142 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 4001 6314 6673 6832 518 Size: 648 1393 1442 2313 359 159 2042 Fragments arranged by size: 2313 2042 1442 1393 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3142 3151 4225 4772 4851 4960 4968 4979 Size: 1536 9 1074 547 79 109 8 11 Cuts at: 4979 5263 5772 6449 7597 7842 1606 Size: 284 509 677 1148 245 2120 Fragments arranged by size: 2120 1536 1148 1074 677 547 509 284 245 109 79 11 9 8 NaeI GCC'GGC Cuts at: 2271 2479 2985 3510 4668 2271 Size: 208 506 525 1158 5959 Fragments arranged by size: 5959 1158 525 506 208 NarI GG'CG_CC Cuts at: 4039 4998 4039 Size: 959 7397 NcoI C'CATG_G Cuts at: 513 1065 2343 3321 513 Size: 552 1278 978 5548 Fragments arranged by size: 5548 1278 978 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8356 NgoMIV G'CCGG_C Cuts at: 2269 2477 2983 3508 4666 2269 Size: 208 506 525 1158 5959 Fragments arranged by size: 5959 1158 525 506 208 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8356 NotI GC'GGCC_GC Cuts at: 5803 5803 Size: 8356 NspI r_CATG'y Cuts at: 1132 1229 4006 5714 7259 1132 Size: 97 2777 1708 1545 2229 Fragments arranged by size: 2777 2229 1708 1545 97 PasI CC'CwG_GG Cuts at: 1348 3709 3961 4164 4455 4465 1348 Size: 2361 252 203 291 10 5239 Fragments arranged by size: 5239 2361 291 252 203 10 PciI A'CATG_T Cuts at: 7255 7255 Size: 8356 PflMI CCAn_nnn'nTGG Cuts at: 1108 4679 5139 1108 Size: 3571 460 4325 Fragments arranged by size: 4325 3571 460 PfoI T'CCnGG_A Cuts at: 2433 3410 3483 2433 Size: 977 73 7306 Fragments arranged by size: 7306 977 73 PmeI GTTT'AAAC Cuts at: 5660 5660 Size: 8356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6425 6457 7938 7970 6425 Size: 32 1481 32 6811 Fragments arranged by size: 6811 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3181 3534 4096 4313 4714 3181 Size: 353 562 217 401 6823 Fragments arranged by size: 6823 562 401 353 217 PshAI GACnn'nnGTC Cuts at: 1613 3330 1613 Size: 1717 6639 PsiI TTA'TAA Cuts at: 5926 5926 Size: 8356 PspOMI G'GGCC_C Cuts at: 2484 3985 4258 4468 4606 4630 5215 5359 2484 Size: 1501 273 210 138 24 585 144 5481 Fragments arranged by size: 5481 1501 585 273 210 144 138 24 PstI C_TGCA'G Cuts at: 830 2187 3156 5358 5708 830 Size: 1357 969 2202 350 3478 Fragments arranged by size: 3478 2202 1357 969 350 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8356 PvuII CAG'CTG Cuts at: 3142 3151 4225 4772 4960 4968 3142 Size: 9 1074 547 188 8 6530 Fragments arranged by size: 6530 1074 547 188 9 8 SacI G_AGCT'C Cuts at: 4061 5504 4061 Size: 1443 6913 SalI G'TCGA_C Cuts at: 1936 5698 1936 Size: 3762 4594 SanDI GG'GwC_CC Cuts at: 3534 4714 3534 Size: 1180 7176 SapI GCTCTTCn'nnn_ Cuts at: 4268 5285 4268 Size: 1017 7339 SbfI CC_TGCA'GG Cuts at: 5708 5708 Size: 8356 ScaI AGT'ACT Cuts at: 6604 8316 6604 Size: 1712 6644 SfcI C'TryA_G Cuts at: 826 1045 2095 2183 2361 3152 3516 5354 5704 Size: 219 1050 88 178 791 364 1838 350 Cuts at: 5704 6839 7520 7711 826 Size: 1135 681 191 1471 Fragments arranged by size: 1838 1471 1135 1050 791 681 364 350 219 191 178 88 SfiI GGCCn_nnn'nGGCC Cuts at: 2483 2483 Size: 8356 SfoI GGC'GCC Cuts at: 4040 4999 4040 Size: 959 7397 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8356 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8356 SmaI CCC'GGG Cuts at: 1872 2159 2473 5055 5225 5683 1872 Size: 287 314 2582 170 458 4545 Fragments arranged by size: 4545 2582 458 314 287 170 SmlI C'TyrA_G Cuts at: 820 1017 1943 4142 6460 7361 7623 820 Size: 197 926 2199 2318 901 262 1553 Fragments arranged by size: 2318 2199 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5653 6147 493 Size: 5160 494 2702 Fragments arranged by size: 5160 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8356 SphI G_CATG'C Cuts at: 1132 5714 1132 Size: 4582 3774 SspI AAT'ATT Cuts at: 5 52 6280 5 Size: 47 6228 2081 Fragments arranged by size: 6228 2081 47 StuI AGG'CCT Cuts at: 1517 2075 1517 Size: 558 7798 StyI C'CwwG_G Cuts at: 513 1065 2343 2943 3321 4569 4866 5577 5795 Size: 552 1278 600 378 1248 297 711 218 Cuts at: 5795 513 Size: 3074 Fragments arranged by size: 3074 1278 1248 711 600 552 378 297 218 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3304 5131 5288 6383 6400 6553 6738 Size: 1255 1724 1827 157 1095 17 153 185 Cuts at: 6738 7183 325 Size: 445 1498 Fragments arranged by size: 1827 1724 1498 1255 1095 445 185 157 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6602 8314 96 Size: 274 80 33 51 1443 4625 1712 138 Fragments arranged by size: 4625 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8316 8348 8316 Size: 32 8324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8356 XbaI T'CTAG_A Cuts at: 5692 5692 Size: 8356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2756 3319 3958 2756 Size: 563 639 7154 Fragments arranged by size: 7154 639 563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8356 XmaI C'CCGG_G Cuts at: 1870 2157 2471 5053 5223 5681 1870 Size: 287 314 2582 170 458 4545 Fragments arranged by size: 4545 2582 458 314 287 170 XmnI GAAnn'nnTTC Cuts at: 2718 4448 4734 6485 2718 Size: 1730 286 1751 4589 Fragments arranged by size: 4589 1751 1730 286 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6164 276 Size: 53 83 186 936 4630 2468 Fragments arranged by size: 4630 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SrfI SwaI