(Circular) (Six-base) MAPSORT of: pFN21ASDA0645.seq Check: 3174 from: 1 to: 9445 pFN21ASDA0645 9445 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3470 3648 3470 Size: 178 9267 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7255 278 Size: 53 83 186 936 5719 2468 Fragments arranged by size: 5719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2040 2292 6788 1937 Size: 103 252 4496 4594 Fragments arranged by size: 4594 4496 252 103 Acc65I G'GTAC_C Cuts at: 6766 6766 Size: 9445 AclI AA'CG_TT Cuts at: 1563 4571 7572 7945 1563 Size: 3008 3001 373 3063 Fragments arranged by size: 3063 3008 3001 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4060 4610 4777 4806 4832 6668 7508 8892 Size: 2428 550 167 29 26 1836 840 1384 Cuts at: 8892 9160 1632 Size: 268 1917 Fragments arranged by size: 2428 1917 1836 1384 840 550 268 167 29 26 AflII C'TTAA_G Cuts at: 820 1017 2195 820 Size: 197 1178 8070 Fragments arranged by size: 8070 1178 197 AflIII A'CryG_T Cuts at: 1203 2393 6540 8344 9321 1203 Size: 1190 4147 1804 977 1327 Fragments arranged by size: 4147 1804 1327 1190 977 AgeI A'CCGG_T Cuts at: 8276 8276 Size: 9445 AhdI GACnn_n'nnGTC Cuts at: 4060 4228 8174 4060 Size: 168 3946 5331 Fragments arranged by size: 5331 3946 168 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9445 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3488 6747 1184 Size: 2304 3259 3882 Fragments arranged by size: 3882 3259 2304 AlwNI CAG_nnn'CTG Cuts at: 3513 4291 4671 5425 7188 8760 3513 Size: 778 380 754 1763 1572 4198 Fragments arranged by size: 4198 1763 1572 778 754 380 ApaI G_GGCC'C Cuts at: 3448 5055 3448 Size: 1607 7838 ApaLI G'TGCA_C Cuts at: 3732 5840 7502 8658 3732 Size: 2108 1662 1156 4519 Fragments arranged by size: 4519 2108 1662 1156 ApoI r'AATT_y Cuts at: 1493 2720 4715 6754 6980 7132 9241 1493 Size: 1227 1995 2039 226 152 2109 1697 Fragments arranged by size: 2109 2039 1995 1697 1227 226 152 AseI AT'TA_AT Cuts at: 160 1032 7999 160 Size: 872 6967 1606 Fragments arranged by size: 6967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9445 AvaI C'yCGr_G Cuts at: 1870 1943 3035 3350 3441 3684 6770 1870 Size: 73 1092 315 91 243 3086 4545 Fragments arranged by size: 4545 3086 1092 315 243 91 73 AvrII C'CTAG_G Cuts at: 3598 4136 3598 Size: 538 8907 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2490 2523 5552 5585 2490 Size: 33 3029 33 6350 Fragments arranged by size: 6350 3029 33 33 BamHI G'GATC_C Cuts at: 3970 5589 6775 7142 3970 Size: 1619 1186 367 6273 Fragments arranged by size: 6273 1619 1186 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5297 6766 8221 618 Size: 325 217 627 3510 1469 1455 1842 Fragments arranged by size: 3510 1842 1469 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3448 5055 5195 5727 5838 6764 1391 Size: 2057 1607 140 532 111 926 4072 Fragments arranged by size: 4072 2057 1607 926 532 140 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2784 4794 928 Size: 971 885 2010 5579 Fragments arranged by size: 5579 2010 971 885 BbvCI CC'TCA_GC Cuts at: 2923 5110 2923 Size: 2187 7258 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7636 7670 1903 Size: 34 5699 34 3678 Fragments arranged by size: 5699 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2874 3665 3785 4641 7337 8547 2874 Size: 791 120 856 2696 1210 3772 Fragments arranged by size: 3772 2696 1210 856 791 120 BclI T'GATC_A Cuts at: 1548 3027 4602 1548 Size: 1479 1575 6391 Fragments arranged by size: 6391 1575 1479 BfrBI ATG'CAT Cuts at: 5657 6724 5657 Size: 1067 8378 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5825 5884 8056 Size: 107 122 71 631 605 4153 59 2172 Cuts at: 8056 136 Size: 1525 Fragments arranged by size: 4153 2172 1525 631 605 122 107 71 59 BglII A'GATC_T Cuts at: 3467 9440 3467 Size: 5973 3472 BlpI GC'TnA_GC Cuts at: 4109 4484 6862 4109 Size: 375 2378 6692 Fragments arranged by size: 6692 2378 375 Bme1580I G_kGCm'C Cuts at: 1790 3448 3736 5055 5844 6241 6736 7506 8662 Size: 1658 288 1319 789 397 495 770 1156 Cuts at: 8662 1790 Size: 2573 Fragments arranged by size: 2573 1658 1319 1156 789 770 495 397 288 BmgBI CAC'GTC Cuts at: 1318 6007 1318 Size: 4689 4756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3018 3442 5058 Size: 416 53 83 399 24 1601 424 1616 Cuts at: 5058 5084 5194 6176 6204 6334 6580 7176 8134 Size: 26 110 982 28 130 246 596 958 Cuts at: 8134 442 Size: 1753 Fragments arranged by size: 1753 1616 1601 982 958 596 424 416 399 246 130 110 83 53 28 26 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9445 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4717 4717 Size: 9445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2963 3712 3716 4019 4167 4754 4840 5093 Size: 1724 749 4 303 148 587 86 253 Cuts at: 5093 5386 6050 6592 6734 8105 1239 Size: 293 664 542 142 1371 2579 Fragments arranged by size: 2579 1724 1371 749 664 587 542 303 293 253 148 142 86 4 Bpu10I CC'TnA_GC Cuts at: 2923 5110 2923 Size: 2187 7258 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2229 2889 3399 6260 7570 8435 8733 2229 Size: 660 510 2861 1310 865 298 2941 Fragments arranged by size: 2941 2861 1310 865 660 510 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2254 8108 882 Size: 617 755 5854 2219 Fragments arranged by size: 5854 2219 755 617 BsaAI yAC'GTr Cuts at: 493 1204 5581 6742 7236 493 Size: 711 4377 1161 494 2702 Fragments arranged by size: 4377 2702 1161 711 494 BsaBI GATnn'nnATC Cuts at: 9439 9439 Size: 9445 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3402 7252 7634 8269 Size: 53 83 186 936 1869 3850 382 635 Cuts at: 8269 275 Size: 1451 Fragments arranged by size: 3850 1869 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3899 7878 8276 8550 8697 9108 3899 Size: 3979 398 274 147 411 4236 Fragments arranged by size: 4236 3979 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2121 2151 3578 3608 4532 Size: 30 165 30 718 30 1427 30 924 Cuts at: 4532 4562 4730 4760 5266 5296 1178 Size: 30 168 30 506 30 5327 Fragments arranged by size: 5327 1427 924 718 506 168 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3586 3973 4103 4550 4553 4751 5380 6566 Size: 2400 387 130 447 3 198 629 1186 Cuts at: 6566 1186 Size: 4065 Fragments arranged by size: 4065 2400 1186 629 447 387 198 130 3 BseYI C'CCAG_C Cuts at: 4380 4707 6240 8648 9144 4380 Size: 327 1533 2408 496 4681 Fragments arranged by size: 4681 2408 1533 496 327 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 3626 3780 4143 4388 4460 4938 Size: 573 1083 807 154 363 245 72 478 Cuts at: 4938 5067 6058 6077 1163 Size: 129 991 19 4531 Fragments arranged by size: 4531 1083 991 807 573 478 363 245 154 129 72 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5731 6895 7656 8684 1540 Size: 275 186 3730 1164 761 1028 2301 Fragments arranged by size: 3730 2301 1164 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2440 3184 3736 3811 5195 5838 5844 6294 7506 Size: 744 552 75 1384 643 6 450 1212 Cuts at: 7506 7591 8662 2440 Size: 85 1071 3223 Fragments arranged by size: 3223 1384 1212 1071 744 643 552 450 85 75 6 BsiWI C'GTAC_G Cuts at: 6738 6738 Size: 9445 BsmI GAATG_Cn' Cuts at: 4980 5065 6076 6339 6956 7049 4980 Size: 85 1011 263 617 93 7376 Fragments arranged by size: 7376 1011 617 263 93 85 BsmBI CGTCTCn'nnnn_ Cuts at: 4418 4705 4418 Size: 287 9158 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2440 3184 3448 3736 3811 5055 5195 Size: 399 650 744 264 288 75 1244 140 Cuts at: 5195 5727 5838 5844 6241 6294 6736 6764 7506 Size: 532 111 6 397 53 442 28 742 Cuts at: 7506 7591 8662 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1244 1071 744 742 650 532 442 399 397 288 264 140 111 85 75 53 28 6 BspEI T'CCGG_A Cuts at: 3899 9108 3899 Size: 5209 4236 BspHI T'CATG_A Cuts at: 2662 3069 4012 7334 9064 2662 Size: 407 943 3322 1730 3043 Fragments arranged by size: 3322 3043 1730 943 407 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2173 3470 3648 4115 4375 6800 844 Size: 1329 1297 178 467 260 2425 3489 Fragments arranged by size: 3489 2425 1329 1297 467 260 178 BsrBI CCG'CTC Cuts at: 1394 1823 6556 7332 1394 Size: 429 4733 776 3507 Fragments arranged by size: 4733 3507 776 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6354 7940 8114 65 Size: 1183 5106 1586 174 1396 Fragments arranged by size: 5106 1586 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4778 5883 6024 6684 8089 8276 1700 Size: 110 2968 1105 141 660 1405 187 2869 Fragments arranged by size: 2968 2869 1405 1105 660 187 141 110 BsrGI T'GTAC_A Cuts at: 96 6376 96 Size: 6280 3165 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9445 BssSI C'ACGA_G Cuts at: 5837 7505 8517 5837 Size: 1668 1012 6765 Fragments arranged by size: 6765 1668 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5629 794 Size: 4835 4610 BstBI TT'CG_AA Cuts at: 2007 8249 2007 Size: 6242 3203 BstEII G'GTnAC_C Cuts at: 1184 6106 1184 Size: 4922 4523 BstXI CCAn_nnnn'nTGG Cuts at: 5242 5354 5242 Size: 112 9333 BstYI r'GATC_y Cuts at: 1972 3467 3915 3970 4650 5466 5589 6775 7142 Size: 1495 448 55 680 816 123 1186 367 Cuts at: 7142 7527 7544 8996 9082 9094 9440 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1495 1452 1186 816 680 448 385 367 346 123 86 55 17 12 Bsu36I CC'TnA_GG Cuts at: 2298 4533 2298 Size: 2235 7210 BtgI C'CryG_G Cuts at: 513 1065 2353 5328 6052 513 Size: 552 1288 2975 724 3906 Fragments arranged by size: 3906 2975 1288 724 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3219 4979 5618 487 Size: 683 56 1993 1760 639 4314 Fragments arranged by size: 4314 1993 1760 683 639 56 BtsI GCAGTG_nn' Cuts at: 2083 3551 3763 4888 6203 6961 7755 7775 2083 Size: 1468 212 1125 1315 758 794 20 3753 Fragments arranged by size: 3753 1468 1315 1125 794 758 212 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9410 DraI TTT'AAA Cuts at: 6749 6749 Size: 9445 DraIII CAC_nnn'GTG Cuts at: 4582 4582 Size: 9445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8452 809 Size: 507 7136 1802 Fragments arranged by size: 7136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2350 3334 5607 Size: 54 1425 50 275 18 520 984 2273 Cuts at: 5607 5736 5885 6892 7781 9410 8 Size: 129 149 1007 889 1629 43 Fragments arranged by size: 2273 1629 1425 1007 984 889 520 275 149 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6892 1537 Size: 275 5080 4090 Fragments arranged by size: 5080 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2806 2858 4389 5183 5631 5826 6128 6251 Size: 1453 52 1531 794 448 195 302 123 Cuts at: 6251 6347 7375 1353 Size: 96 1028 3423 Fragments arranged by size: 3423 1531 1453 1028 794 448 302 195 123 96 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3959 5185 5689 6526 8032 8406 Size: 1064 138 2370 1226 504 837 1506 374 Cuts at: 8406 8552 387 Size: 146 1280 Fragments arranged by size: 2370 1506 1280 1226 1064 837 504 374 146 138 EcoICRI GAG'CTC Cuts at: 5193 5836 5193 Size: 643 8802 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2963 3712 3716 4019 4060 4167 4610 Size: 393 1331 749 4 303 41 107 443 Cuts at: 4610 4754 4777 4806 4832 4840 5093 5386 6050 Size: 144 23 29 26 8 253 293 664 Cuts at: 6050 6592 6668 6734 7508 8105 8892 9160 1239 Size: 542 76 66 774 597 787 268 1524 Fragments arranged by size: 1524 1331 787 774 749 664 597 542 443 393 303 293 268 253 144 107 76 66 41 29 26 23 8 4 EcoNI CCTnn'n_nnAGG Cuts at: 6344 6344 Size: 9445 EcoO109I rG'GnC_Cy Cuts at: 4140 4288 4824 4911 5524 6194 4140 Size: 148 536 87 613 670 7391 Fragments arranged by size: 7391 670 613 536 148 87 EcoRI G'AATT_C Cuts at: 4715 6754 7132 4715 Size: 2039 378 7028 Fragments arranged by size: 7028 2039 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2083 2083 Size: 9445 FspI TGC'GCA Cuts at: 7951 7951 Size: 9445 HaeII r_GCGC'y Cuts at: 1678 3871 6607 6615 8592 1678 Size: 2193 2736 8 1977 2531 Fragments arranged by size: 2736 2531 2193 1977 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2251 2259 2283 2693 Size: 32 135 32 655 24 8 24 410 Cuts at: 2693 2725 3478 3509 3510 3541 3578 3610 3894 Size: 32 753 31 1 31 37 32 284 Cuts at: 3894 3926 4046 4078 4214 4246 5399 5431 8086 Size: 32 120 32 136 32 1153 32 2655 Cuts at: 8086 8118 8160 8192 1373 Size: 32 42 32 2626 Fragments arranged by size: 2655 2626 1153 753 655 410 284 136 135 120 42 37 32 32 32 32 32 32 32 32 32 32 31 31 24 24 8 1 HincII GTy'rAC Cuts at: 669 1657 1938 5079 6004 6789 7035 669 Size: 988 281 3141 925 785 246 3079 Fragments arranged by size: 3141 3079 988 925 785 281 246 HindIII A'AGCT_T Cuts at: 748 2123 3848 748 Size: 1375 1725 6345 Fragments arranged by size: 6345 1725 1375 HpaI GTT'AAC Cuts at: 7035 7035 Size: 9445 KpnI G_GTAC'C Cuts at: 6770 6770 Size: 9445 MfeI C'AATT_G Cuts at: 7044 7044 Size: 9445 MluI A'CGCG_T Cuts at: 9321 9321 Size: 9445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3882 3998 4195 5109 5271 8559 8743 1305 Size: 2577 116 197 914 162 3288 184 2007 Fragments arranged by size: 3288 2577 2007 914 197 184 162 116 MscI TGG'CCA Cuts at: 10 64 1489 1832 5609 5738 10 Size: 54 1425 343 3777 129 3717 Fragments arranged by size: 3777 3717 1425 343 129 54 MslI CAynn'nnrTG Cuts at: 518 1166 3620 3648 5240 5285 6729 7403 7762 Size: 648 2454 28 1592 45 1444 674 359 Cuts at: 7762 7921 518 Size: 159 2042 Fragments arranged by size: 2454 2042 1592 1444 674 648 359 159 45 28 MspA1I CmG'CkG Cuts at: 1606 3088 3960 4483 5174 5425 5506 6042 6267 Size: 1482 872 523 691 251 81 536 225 Cuts at: 6267 6466 6502 6861 7538 8686 8931 1606 Size: 199 36 359 677 1148 245 2120 Fragments arranged by size: 2120 1482 1148 872 691 677 536 523 359 251 245 225 199 81 36 NaeI GCC'GGC Cuts at: 4780 5885 4780 Size: 1105 8340 NcoI C'CATG_G Cuts at: 513 1065 5328 6052 513 Size: 552 4263 724 3906 Fragments arranged by size: 4263 3906 724 552 NdeI CA'TA_TG Cuts at: 387 5312 387 Size: 4925 4520 NgoMIV G'CCGG_C Cuts at: 4778 5883 4778 Size: 1105 8340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9445 NotI GC'GGCC_GC Cuts at: 6892 6892 Size: 9445 NsiI A_TGCA'T Cuts at: 5659 6726 5659 Size: 1067 8378 NspI r_CATG'y Cuts at: 1132 1229 2397 3149 3779 4063 5657 6544 6728 Size: 97 1168 752 630 284 1594 887 184 Cuts at: 6728 6803 8348 1132 Size: 75 1545 2229 Fragments arranged by size: 2229 1594 1545 1168 887 752 630 284 184 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9445 PciI A'CATG_T Cuts at: 2393 6540 8344 2393 Size: 4147 1804 3494 Fragments arranged by size: 4147 3494 1804 PflMI CCAn_nnn'nTGG Cuts at: 1108 2416 5329 5760 6600 1108 Size: 1308 2913 431 840 3953 Fragments arranged by size: 3953 2913 1308 840 431 PfoI T'CCnGG_A Cuts at: 3693 4941 5469 6345 3693 Size: 1248 528 876 6793 Fragments arranged by size: 6793 1248 876 528 PmeI GTTT'AAAC Cuts at: 6749 6749 Size: 9445 PmlI CAC'GTG Cuts at: 5581 5581 Size: 9445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5098 5130 7514 7546 9027 9059 5098 Size: 32 2384 32 1481 32 5484 Fragments arranged by size: 5484 2384 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4140 4824 4140 Size: 684 8761 PshAI GACnn'nnGTC Cuts at: 1613 2675 5498 1613 Size: 1062 2823 5560 Fragments arranged by size: 5560 2823 1062 PsiI TTA'TAA Cuts at: 7015 7015 Size: 9445 PspOMI G'GGCC_C Cuts at: 3444 5051 3444 Size: 1607 7838 PstI C_TGCA'G Cuts at: 830 3467 3991 4296 6465 6797 830 Size: 2637 524 305 2169 332 3478 Fragments arranged by size: 3478 2637 2169 524 332 305 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9445 PvuII CAG'CTG Cuts at: 5174 5425 5506 6042 6267 6466 5174 Size: 251 81 536 225 199 8153 Fragments arranged by size: 8153 536 251 225 199 81 SacI G_AGCT'C Cuts at: 5195 5838 5195 Size: 643 8802 SalI G'TCGA_C Cuts at: 1936 6787 1936 Size: 4851 4594 SapI GCTCTTCn'nnn_ Cuts at: 5183 5631 5826 6251 5183 Size: 448 195 425 8377 Fragments arranged by size: 8377 448 425 195 SbfI CC_TGCA'GG Cuts at: 6797 6797 Size: 9445 ScaI AGT'ACT Cuts at: 6120 7693 9405 6120 Size: 1573 1712 6160 Fragments arranged by size: 6160 1712 1573 SexAI A'CCwGG_T Cuts at: 3784 3784 Size: 9445 SfcI C'TryA_G Cuts at: 826 1045 2656 3463 3987 4292 4501 4663 6461 Size: 219 1611 807 524 305 209 162 1798 Cuts at: 6461 6793 7928 8609 8800 826 Size: 332 1135 681 191 1471 Fragments arranged by size: 1798 1611 1471 1135 807 681 524 332 305 219 209 191 162 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9445 SmaI CCC'GGG Cuts at: 1872 3037 3443 6772 1872 Size: 1165 406 3329 4545 Fragments arranged by size: 4545 3329 1165 406 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 2244 2904 3414 3684 6275 Size: 197 926 252 49 660 510 270 2591 Cuts at: 6275 7549 8450 8712 820 Size: 1274 901 262 1553 Fragments arranged by size: 2591 1553 1274 926 901 660 510 270 262 252 197 49 SnaBI TAC'GTA Cuts at: 493 6742 7236 493 Size: 6249 494 2702 Fragments arranged by size: 6249 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9445 SphI G_CATG'C Cuts at: 1132 6728 6803 1132 Size: 5596 75 3774 Fragments arranged by size: 5596 3774 75 SspI AAT'ATT Cuts at: 5 52 6389 7369 5 Size: 47 6337 980 2081 Fragments arranged by size: 6337 2081 980 47 StuI AGG'CCT Cuts at: 1517 4512 1517 Size: 2995 6450 StyI C'CwwG_G Cuts at: 513 1065 3598 3666 4136 5328 6052 6136 6884 Size: 552 2533 68 470 1192 724 84 748 Cuts at: 6884 513 Size: 3074 Fragments arranged by size: 3074 2533 1192 748 724 552 470 84 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 3018 3032 4699 5745 7472 7489 Size: 1255 843 595 14 1667 1046 1727 17 Cuts at: 7489 7642 7827 8272 325 Size: 153 185 445 1498 Fragments arranged by size: 1727 1667 1498 1255 1046 843 595 445 185 153 17 14 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4740 6118 6376 Size: 274 80 33 51 1443 2763 1378 258 Cuts at: 6376 7691 9403 96 Size: 1315 1712 138 Fragments arranged by size: 2763 1712 1443 1378 1315 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3096 3107 3128 3139 4022 4054 4730 4762 9405 Size: 11 21 11 883 32 676 32 4643 Cuts at: 9405 9437 3096 Size: 32 3104 Fragments arranged by size: 4643 3104 883 676 32 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9445 XbaI T'CTAG_A Cuts at: 2687 4159 6781 2687 Size: 1472 2622 5351 Fragments arranged by size: 5351 2622 1472 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5068 5287 5068 Size: 219 9226 XhoI C'TCGA_G Cuts at: 1943 3684 1943 Size: 1741 7704 XmaI C'CCGG_G Cuts at: 1870 3035 3441 6770 1870 Size: 1165 406 3329 4545 Fragments arranged by size: 4545 3329 1165 406 XmnI GAAnn'nnTTC Cuts at: 2615 6542 6653 7574 2615 Size: 3927 111 921 4486 Fragments arranged by size: 4486 3927 921 111 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7253 276 Size: 53 83 186 936 5719 2468 Fragments arranged by size: 5719 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BstZ17I EcoRV FseI FspAI KasI NarI NruI PacI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI