(Circular) (Six-base) MAPSORT of: pFN21ASDA0644.seq Check: 5226 from: 1 to: 7162 pFN21ASDA0644 7162 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2784 3358 2784 Size: 574 6588 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4972 278 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3933 4101 4505 1937 Size: 1996 168 404 4594 Fragments arranged by size: 4594 1996 404 168 Acc65I G'GTAC_C Cuts at: 4483 4483 Size: 7162 AclI AA'CG_TT Cuts at: 1563 5289 5662 1563 Size: 3726 373 3063 Fragments arranged by size: 3726 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3152 5225 6609 6877 1632 Size: 1520 2073 1384 268 1917 Fragments arranged by size: 2073 1917 1520 1384 268 AfeI AGC'GCT Cuts at: 2111 3137 3463 2111 Size: 1026 326 5810 Fragments arranged by size: 5810 1026 326 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6965 AflIII A'CryG_T Cuts at: 1203 4242 6061 7038 1203 Size: 3039 1819 977 1327 Fragments arranged by size: 3039 1819 1327 977 AgeI A'CCGG_T Cuts at: 5993 5993 Size: 7162 AhdI GACnn_n'nnGTC Cuts at: 5891 5891 Size: 7162 AleI CACnn'nnGTG Cuts at: 1166 4283 4331 1166 Size: 3117 48 3997 Fragments arranged by size: 3997 3117 48 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4464 1184 Size: 3280 3882 AlwNI CAG_nnn'CTG Cuts at: 2776 3247 4114 4905 6477 2776 Size: 471 867 791 1572 3461 Fragments arranged by size: 3461 1572 867 791 471 ApaI G_GGCC'C Cuts at: 3488 3533 4225 3488 Size: 45 692 6425 Fragments arranged by size: 6425 692 45 ApaLI G'TGCA_C Cuts at: 2143 5219 6375 2143 Size: 3076 1156 2930 Fragments arranged by size: 3076 2930 1156 ApoI r'AATT_y Cuts at: 1493 4364 4471 4697 4849 6958 1493 Size: 2871 107 226 152 2109 1697 Fragments arranged by size: 2871 2109 1697 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 5716 160 Size: 872 4684 1606 Fragments arranged by size: 4684 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 AvaI C'yCGr_G Cuts at: 1870 1943 2392 2753 3487 3585 4044 4300 4487 Size: 73 449 361 734 98 459 256 187 Cuts at: 4487 1870 Size: 4545 Fragments arranged by size: 4545 734 459 449 361 256 187 98 73 BamHI G'GATC_C Cuts at: 2592 3257 3268 4492 4859 2592 Size: 665 11 1224 367 4895 Fragments arranged by size: 4895 1224 665 367 11 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4277 4483 5938 618 Size: 325 217 627 2490 206 1455 1842 Fragments arranged by size: 2490 1842 1455 627 325 217 206 BanII G_rGCy'C Cuts at: 1391 2097 2160 2195 3012 3488 3533 3612 4225 Size: 706 63 35 817 476 45 79 613 Cuts at: 4225 4481 1391 Size: 256 4072 Fragments arranged by size: 4072 817 706 613 476 256 79 63 45 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2536 3172 928 Size: 971 637 636 4918 Fragments arranged by size: 4918 971 637 636 BbvCI CC'TCA_GC Cuts at: 3280 3280 Size: 7162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5353 5387 1903 Size: 34 3416 34 3678 Fragments arranged by size: 3678 3416 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5054 6264 5054 Size: 1210 5952 BclI T'GATC_A Cuts at: 1548 1548 Size: 7162 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2223 2525 2994 Size: 107 122 71 631 605 551 302 469 Cuts at: 2994 4095 4166 5773 136 Size: 1101 71 1607 1525 Fragments arranged by size: 1607 1525 1101 631 605 551 469 302 122 107 71 71 BglII A'GATC_T Cuts at: 7157 7157 Size: 7162 BlpI GC'TnA_GC Cuts at: 2888 3011 3032 4579 2888 Size: 123 21 1547 5471 Fragments arranged by size: 5471 1547 123 21 Bme1580I G_kGCm'C Cuts at: 1790 2105 2147 2172 2399 3488 3533 3692 4225 Size: 315 42 25 227 1089 45 159 533 Cuts at: 4225 4280 5223 6379 1790 Size: 55 943 1156 2573 Fragments arranged by size: 2573 1156 1089 943 533 315 227 159 55 45 42 25 BmgBI CAC'GTC Cuts at: 1318 2203 2783 1318 Size: 885 580 5697 Fragments arranged by size: 5697 885 580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3709 4893 5851 Size: 416 53 83 399 24 2292 1184 958 Cuts at: 5851 442 Size: 1753 Fragments arranged by size: 2292 1753 1184 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7162 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2356 2356 Size: 7162 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2600 2894 2933 2951 3374 3479 5822 Size: 1265 96 294 39 18 423 105 2343 Cuts at: 5822 1239 Size: 2579 Fragments arranged by size: 2579 2343 1265 423 294 105 96 39 18 Bpu10I CC'TnA_GC Cuts at: 2459 2801 2945 3280 2459 Size: 342 144 335 6341 Fragments arranged by size: 6341 342 335 144 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5287 6152 6450 5287 Size: 865 298 5999 Fragments arranged by size: 5999 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3362 5825 882 Size: 617 1863 2463 2219 Fragments arranged by size: 2463 2219 1863 617 BsaAI yAC'GTr Cuts at: 493 1204 4459 4953 493 Size: 711 3255 494 2702 Fragments arranged by size: 3255 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7156 7156 Size: 7162 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3344 4098 4175 4969 Size: 53 83 186 936 1811 754 77 794 Cuts at: 4969 5351 5986 275 Size: 382 635 1451 Fragments arranged by size: 1811 1451 936 794 754 635 382 186 83 77 53 BsaWI w'CCGG_w Cuts at: 2589 4075 5595 5993 6267 6414 6825 2589 Size: 1486 1520 398 274 147 411 2926 Fragments arranged by size: 2926 1520 1486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3610 3640 4232 4262 1178 Size: 30 165 30 2207 30 592 30 4078 Fragments arranged by size: 4078 2207 592 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2707 3161 3350 3380 1186 Size: 1521 454 189 30 4968 Fragments arranged by size: 4968 1521 454 189 30 BseYI C'CCAG_C Cuts at: 2130 2622 3067 3413 3503 3567 3831 3865 6365 Size: 492 445 346 90 64 264 34 2500 Cuts at: 6365 6861 2130 Size: 496 2431 Fragments arranged by size: 2500 2431 496 492 445 346 264 90 64 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 2906 2954 3206 3539 1163 Size: 573 564 606 48 252 333 4786 Fragments arranged by size: 4786 606 573 564 333 252 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3327 3863 4378 4612 5373 6401 Size: 275 186 1326 536 515 234 761 1028 Cuts at: 6401 1540 Size: 2301 Fragments arranged by size: 2301 1326 1028 761 536 515 275 234 186 BsiHKAI G_wGCw'C Cuts at: 2147 2208 3012 3699 3849 4155 5223 5308 6379 Size: 61 804 687 150 306 1068 85 1071 Cuts at: 6379 2147 Size: 2930 Fragments arranged by size: 2930 1071 1068 804 687 306 150 85 61 BsmI GAATG_Cn' Cuts at: 4673 4766 4673 Size: 93 7069 BsmBI CGTCTCn'nnnn_ Cuts at: 2789 4182 4414 4439 2789 Size: 1393 232 25 5512 Fragments arranged by size: 5512 1393 232 25 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2105 2147 2160 2172 2195 2208 Size: 399 307 8 42 13 12 23 13 Cuts at: 2208 2399 3012 3488 3533 3612 3692 3699 3849 Size: 191 613 476 45 79 80 7 150 Cuts at: 3849 4155 4225 4280 4481 5223 5308 6379 1391 Size: 306 70 55 201 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 476 399 307 306 201 191 150 85 80 79 70 55 45 42 23 13 13 12 8 7 BspEI T'CCGG_A Cuts at: 6825 6825 Size: 7162 BspHI T'CATG_A Cuts at: 5051 6781 5051 Size: 1730 5432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2298 2571 2784 3116 3344 3358 3561 3945 Size: 1454 273 213 332 228 14 203 384 Cuts at: 3945 4517 844 Size: 572 3489 Fragments arranged by size: 3489 1454 572 384 332 273 228 213 203 14 BsrBI CCG'CTC Cuts at: 1394 1823 2310 2949 5049 1394 Size: 429 487 639 2100 3507 Fragments arranged by size: 3507 2100 639 487 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2959 5657 5831 65 Size: 1183 1711 2698 174 1396 Fragments arranged by size: 2698 1711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2997 3117 3165 3650 4280 4317 5806 Size: 110 1187 120 48 485 630 37 1489 Cuts at: 5806 5993 1700 Size: 187 2869 Fragments arranged by size: 2869 1489 1187 630 485 187 120 110 48 37 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7162 BssHII G'CGCG_C Cuts at: 1925 3667 3873 3875 4338 1925 Size: 1742 206 2 463 4749 Fragments arranged by size: 4749 1742 463 206 2 BssSI C'ACGA_G Cuts at: 2054 5222 6234 2054 Size: 3168 1012 2982 Fragments arranged by size: 3168 2982 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7162 BstBI TT'CG_AA Cuts at: 2007 5966 2007 Size: 3959 3203 BstEII G'GTnAC_C Cuts at: 1184 4066 1184 Size: 2882 4280 BstXI CCAn_nnnn'nTGG Cuts at: 3445 3445 Size: 7162 BstYI r'GATC_y Cuts at: 1972 2304 2592 3085 3257 3268 3592 4492 4859 Size: 332 288 493 172 11 324 900 367 Cuts at: 4859 5244 5261 6713 6799 6811 7157 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 900 493 385 367 346 332 324 288 172 86 17 12 11 Bsu36I CC'TnA_GG Cuts at: 2832 2832 Size: 7162 BtgI C'CryG_G Cuts at: 513 1065 2022 2464 3321 3525 4272 513 Size: 552 957 442 857 204 747 3403 Fragments arranged by size: 3403 957 857 747 552 442 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3627 487 Size: 683 56 2401 4022 Fragments arranged by size: 4022 2401 683 56 BtsI GCAGTG_nn' Cuts at: 3194 4051 4678 5472 5492 3194 Size: 857 627 794 20 4864 Fragments arranged by size: 4864 857 794 627 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7162 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7127 DraI TTT'AAA Cuts at: 4466 4466 Size: 7162 DraIII CAC_nnn'GTG Cuts at: 2203 4335 2203 Size: 2132 5030 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6169 809 Size: 507 4853 1802 Fragments arranged by size: 4853 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2688 2733 Size: 54 1425 50 275 18 258 600 45 Cuts at: 2733 2976 2986 3324 3860 3904 3957 4319 4609 Size: 243 10 338 536 44 53 362 290 Cuts at: 4609 5498 7127 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 600 536 362 338 290 275 258 243 54 53 50 45 44 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 3324 3860 4609 1537 Size: 275 1512 536 749 4090 Fragments arranged by size: 4090 1512 749 536 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 5092 1353 Size: 993 2746 3423 Fragments arranged by size: 3423 2746 993 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2953 3872 4225 4279 4367 5749 Size: 1064 138 1364 919 353 54 88 1382 Cuts at: 5749 6123 6269 387 Size: 374 146 1280 Fragments arranged by size: 1382 1364 1280 1064 919 374 353 146 138 88 54 EcoICRI GAG'CTC Cuts at: 3010 3010 Size: 7162 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 2600 2894 2933 2951 3152 3374 Size: 393 872 96 294 39 18 201 222 Cuts at: 3374 3479 5225 5822 6609 6877 1239 Size: 105 1746 597 787 268 1524 Fragments arranged by size: 1746 1524 872 787 597 393 294 268 222 201 105 96 39 18 EcoNI CCTnn'n_nnAGG Cuts at: 2378 4076 2378 Size: 1698 5464 EcoO109I rG'GnC_Cy Cuts at: 2479 2854 3484 4221 4222 2479 Size: 375 630 737 1 5419 Fragments arranged by size: 5419 737 630 375 1 EcoRI G'AATT_C Cuts at: 4364 4471 4849 4364 Size: 107 378 6677 Fragments arranged by size: 6677 378 107 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2632 2656 2632 Size: 24 7138 FspI TGC'GCA Cuts at: 2414 2423 3020 5668 2414 Size: 9 597 2648 3908 Fragments arranged by size: 3908 2648 597 9 HaeII r_GCGC'y Cuts at: 1678 2113 2387 2531 2939 3039 3139 3465 3822 Size: 435 274 144 408 100 100 326 357 Cuts at: 3822 6309 1678 Size: 2487 2531 Fragments arranged by size: 2531 2487 435 408 357 326 274 144 100 100 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2296 2328 3140 3172 3664 Size: 32 135 32 724 32 812 32 492 Cuts at: 3664 3696 3806 3838 5803 5835 5877 5909 1373 Size: 32 110 32 1965 32 42 32 2626 Fragments arranged by size: 2626 1965 812 724 492 135 110 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4102 4138 4506 4752 669 Size: 988 281 2164 36 368 246 3079 Fragments arranged by size: 3079 2164 988 368 281 246 36 HindIII A'AGCT_T Cuts at: 748 748 Size: 7162 HpaI GTT'AAC Cuts at: 4752 4752 Size: 7162 KpnI G_GTAC'C Cuts at: 4487 4487 Size: 7162 MfeI C'AATT_G Cuts at: 4761 4761 Size: 7162 MluI A'CGCG_T Cuts at: 7038 7038 Size: 7162 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2610 3605 3744 3777 6276 6460 1305 Size: 1305 995 139 33 2499 184 2007 Fragments arranged by size: 2499 2007 1305 995 184 139 33 MscI TGG'CCA Cuts at: 10 64 1489 1832 3906 10 Size: 54 1425 343 2074 3266 Fragments arranged by size: 3266 2074 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4283 4331 5120 5479 5638 518 Size: 648 3117 48 789 359 159 2042 Fragments arranged by size: 3117 2042 789 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2086 2410 2466 2704 2896 3247 3296 3527 Size: 480 324 56 238 192 351 49 231 Cuts at: 3527 3571 3745 3865 4111 4578 5255 6403 6648 Size: 44 174 120 246 467 677 1148 245 Cuts at: 6648 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 480 467 351 324 246 245 238 231 192 174 120 56 49 44 NaeI GCC'GGC Cuts at: 2999 3119 3167 3652 2999 Size: 120 48 485 6509 Fragments arranged by size: 6509 485 120 48 NcoI C'CATG_G Cuts at: 513 1065 2022 4272 513 Size: 552 957 2250 3403 Fragments arranged by size: 3403 2250 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7162 NgoMIV G'CCGG_C Cuts at: 2997 3117 3165 3650 2997 Size: 120 48 485 6509 Fragments arranged by size: 6509 485 120 48 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7162 NotI GC'GGCC_GC Cuts at: 4609 4609 Size: 7162 NspI r_CATG'y Cuts at: 1132 1229 4246 4520 6065 1132 Size: 97 3017 274 1545 2229 Fragments arranged by size: 3017 2229 1545 274 97 PasI CC'CwG_GG Cuts at: 1348 2521 1348 Size: 1173 5989 PciI A'CATG_T Cuts at: 4242 6061 4242 Size: 1819 5343 PflMI CCAn_nnn'nTGG Cuts at: 1108 2266 1108 Size: 1158 6004 PmeI GTTT'AAAC Cuts at: 4466 4466 Size: 7162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5231 5263 6744 6776 5231 Size: 32 1481 32 5617 Fragments arranged by size: 5617 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7162 PsiI TTA'TAA Cuts at: 4732 4732 Size: 7162 PspOMI G'GGCC_C Cuts at: 3484 3529 4221 3484 Size: 45 692 6425 Fragments arranged by size: 6425 692 45 PstI C_TGCA'G Cuts at: 830 2295 2748 3355 4443 4514 830 Size: 1465 453 607 1088 71 3478 Fragments arranged by size: 3478 1465 1088 607 453 71 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7162 PvuII CAG'CTG Cuts at: 2896 3247 4111 2896 Size: 351 864 5947 Fragments arranged by size: 5947 864 351 SacI G_AGCT'C Cuts at: 3012 3012 Size: 7162 SacII CC_GC'GG Cuts at: 2467 3528 2467 Size: 1061 6101 SalI G'TCGA_C Cuts at: 1936 4100 4504 1936 Size: 2164 404 4594 Fragments arranged by size: 4594 2164 404 SapI GCTCTTCn'nnn_ Cuts at: 2346 2346 Size: 7162 SbfI CC_TGCA'GG Cuts at: 3355 4514 3355 Size: 1159 6003 ScaI AGT'ACT Cuts at: 5410 7122 5410 Size: 1712 5450 SexAI A'CCwGG_T Cuts at: 3993 3993 Size: 7162 SfcI C'TryA_G Cuts at: 826 1045 2212 2291 2666 2744 3351 4439 4510 Size: 219 1167 79 375 78 607 1088 71 Cuts at: 4510 5645 6326 6517 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 1088 681 607 375 219 191 79 78 71 SfiI GGCCn_nnn'nGGCC Cuts at: 2994 4166 2994 Size: 1172 5990 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7162 SgrAI Cr'CCGG_yG Cuts at: 1700 3650 4280 1700 Size: 1950 630 4582 Fragments arranged by size: 4582 1950 630 SmaI CCC'GGG Cuts at: 1872 2394 3489 4046 4489 1872 Size: 522 1095 557 443 4545 Fragments arranged by size: 4545 1095 557 522 443 SmlI C'TyrA_G Cuts at: 820 1017 1943 5266 6167 6429 820 Size: 197 926 3323 901 262 1553 Fragments arranged by size: 3323 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4459 4953 493 Size: 3966 494 2702 Fragments arranged by size: 3966 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7162 SphI G_CATG'C Cuts at: 1132 4520 1132 Size: 3388 3774 SrfI GCCC'GGGC Cuts at: 3489 3489 Size: 7162 SspI AAT'ATT Cuts at: 5 52 5086 5 Size: 47 5034 2081 Fragments arranged by size: 5034 2081 47 StuI AGG'CCT Cuts at: 1517 2837 3129 1517 Size: 1320 292 5550 Fragments arranged by size: 5550 1320 292 StyI C'CwwG_G Cuts at: 513 1065 2022 2553 4119 4161 4272 4601 513 Size: 552 957 531 1566 42 111 329 3074 Fragments arranged by size: 3074 1566 957 552 531 329 111 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5189 5206 5359 5544 5989 325 Size: 1255 3609 17 153 185 445 1498 Fragments arranged by size: 3609 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 4245 5408 Size: 274 80 33 51 1443 318 1950 1163 Cuts at: 5408 7120 96 Size: 1712 138 Fragments arranged by size: 1950 1712 1443 1163 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4232 4264 7122 7154 4232 Size: 32 2858 32 4240 Fragments arranged by size: 4240 2858 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2540 1316 Size: 1224 5938 XbaI T'CTAG_A Cuts at: 3088 4498 3088 Size: 1410 5752 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3722 3722 Size: 7162 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7162 XmaI C'CCGG_G Cuts at: 1870 2392 3487 4044 4487 1870 Size: 522 1095 557 443 4545 Fragments arranged by size: 4545 1095 557 522 443 XmnI GAAnn'nnTTC Cuts at: 2615 5291 2615 Size: 2676 4486 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4970 276 Size: 53 83 186 936 3436 2468 Fragments arranged by size: 3436 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SfoI SwaI