Restriction map for
pFN21ASDA0644
(Linear) (Six-base) MAPSORT of: KIAA0644.seq Check: 8924 from: 1 to: 2433
KIAA0644.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 761 1335 2433
Size: 761 574 1098
Fragments arranged by size:
1098 761 574
AccI GT'mk_AC
Cuts at: 0 1910 2078 2433
Size: 1910 168 355
Fragments arranged by size:
1910 355 168
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 2433
Size: 1129 1304
AfeI AGC'GCT
Cuts at: 0 88 1114 1440 2433
Size: 88 1026 326 993
Fragments arranged by size:
1026 993 326 88
AflIII A'CryG_T
Cuts at: 0 2219 2433
Size: 2219 214
AleI CACnn'nnGTG
Cuts at: 0 2260 2308 2433
Size: 2260 48 125
Fragments arranged by size:
2260 125 48
AlwNI CAG_nnn'CTG
Cuts at: 0 753 1224 2091 2433
Size: 753 471 867 342
Fragments arranged by size:
867 753 471 342
ApaI G_GGCC'C
Cuts at: 0 1465 1510 2202 2433
Size: 1465 45 692 231
Fragments arranged by size:
1465 692 231 45
ApaLI G'TGCA_C
Cuts at: 0 120 2433
Size: 120 2313
ApoI r'AATT_y
Cuts at: 0 2341 2433
Size: 2341 92
AvaI C'yCGr_G
Cuts at: 0 369 730 1464 1562 2021 2277 2433
Size: 369 361 734 98 459 256 156
Fragments arranged by size:
734 459 369 361 256 156 98
BamHI G'GATC_C
Cuts at: 0 569 1234 1245 2433
Size: 569 665 11 1188
Fragments arranged by size:
1188 665 569 11
BanI G'GyrC_C
Cuts at: 0 2254 2433
Size: 2254 179
BanII G_rGCy'C
Cuts at: 0 74 137 172 989 1465 1510 1589 2202
Size: 74 63 35 817 476 45 79 613
Cuts at: 2202 2433
Size: 231
Fragments arranged by size:
817 613 476 231 79 74 63 45
35
BbsI GAAGACnn'nnnn_
Cuts at: 0 513 1149 2433
Size: 513 636 1284
Fragments arranged by size:
1284 636 513
BbvCI CC'TCA_GC
Cuts at: 0 1257 2433
Size: 1257 1176
BglI GCCn_nnn'nGGC
Cuts at: 0 200 502 971 2072 2143 2433
Size: 200 302 469 1101 71 290
Fragments arranged by size:
1101 469 302 290 200 71
BlpI GC'TnA_GC
Cuts at: 0 865 988 1009 2433
Size: 865 123 21 1424
Fragments arranged by size:
1424 865 123 21
Bme1580I G_kGCm'C
Cuts at: 0 82 124 149 376 1465 1510 1669 2202
Size: 82 42 25 227 1089 45 159 533
Cuts at: 2202 2257 2433
Size: 55 176
Fragments arranged by size:
1089 533 227 176 159 82 55 45
42 25
BmgBI CAC'GTC
Cuts at: 0 180 760 2433
Size: 180 580 1673
Fragments arranged by size:
1673 580 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 1686 2433
Size: 1686 747
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 333 2433
Size: 333 2100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 577 871 910 928 1351 1456 2433
Size: 481 96 294 39 18 423 105 977
Fragments arranged by size:
977 481 423 294 105 96 39 18
Bpu10I CC'TnA_GC
Cuts at: 0 436 778 922 1257 2433
Size: 436 342 144 335 1176
Fragments arranged by size:
1176 436 342 335 144
BsaI GGTCTCn'nnnn_
Cuts at: 0 1339 2433
Size: 1339 1094
BsaHI Gr'CG_yC
Cuts at: 0 1321 2075 2152 2433
Size: 1321 754 77 281
Fragments arranged by size:
1321 754 281 77
BsaWI w'CCGG_w
Cuts at: 0 566 2052 2433
Size: 566 1486 381
Fragments arranged by size:
1486 566 381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1587 1617 2209 2239 2433
Size: 1587 30 592 30 194
Fragments arranged by size:
1587 592 194 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 684 1138 1327 1357 2433
Size: 684 454 189 30 1076
Fragments arranged by size:
1076 684 454 189 30
BseYI C'CCAG_C
Cuts at: 0 107 599 1044 1390 1480 1544 1808 1842
Size: 107 492 445 346 90 64 264 34
Cuts at: 1842 2433
Size: 591
Fragments arranged by size:
591 492 445 346 264 107 90 64
34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 883 931 1183 1516 2433
Size: 277 606 48 252 333 917
Fragments arranged by size:
917 606 333 277 252 48
BsiEI CG_ry'CG
Cuts at: 0 1304 1840 2355 2433
Size: 1304 536 515 78
Fragments arranged by size:
1304 536 515 78
BsiHKAI G_wGCw'C
Cuts at: 0 124 185 989 1676 1826 2132 2433
Size: 124 61 804 687 150 306 301
Fragments arranged by size:
804 687 306 301 150 124 61
BsmBI CGTCTCn'nnnn_
Cuts at: 0 766 2159 2391 2416 2433
Size: 766 1393 232 25 17
Fragments arranged by size:
1393 766 232 25 17
Bsp1286I G_dGCh'C
Cuts at: 0 74 82 124 137 149 172 185 376
Size: 74 8 42 13 12 23 13 191
Cuts at: 376 989 1465 1510 1589 1669 1676 1826 2132
Size: 613 476 45 79 80 7 150 306
Cuts at: 2132 2202 2257 2433
Size: 70 55 176
Fragments arranged by size:
613 476 306 191 176 150 80 79
74 70 55 45 42 23 13 13
12 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 548 761 1093 1321 1335 1538 1922
Size: 275 273 213 332 228 14 203 384
Cuts at: 1922 2433
Size: 511
Fragments arranged by size:
511 384 332 275 273 228 213 203
14
BsrBI CCG'CTC
Cuts at: 0 287 926 2433
Size: 287 639 1507
Fragments arranged by size:
1507 639 287
BsrDI GCAATG_nn'
Cuts at: 0 936 2433
Size: 936 1497
BsrFI r'CCGG_y
Cuts at: 0 974 1094 1142 1627 2257 2294 2433
Size: 974 120 48 485 630 37 139
Fragments arranged by size:
974 630 485 139 120 48 37
BssHII G'CGCG_C
Cuts at: 0 1644 1850 1852 2315 2433
Size: 1644 206 2 463 118
Fragments arranged by size:
1644 463 206 118 2
BssSI C'ACGA_G
Cuts at: 0 31 2433
Size: 31 2402
BstEII G'GTnAC_C
Cuts at: 0 2043 2433
Size: 2043 390
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1422 2433
Size: 1422 1011
BstYI r'GATC_y
Cuts at: 0 281 569 1062 1234 1245 1569 2433
Size: 281 288 493 172 11 324 864
Fragments arranged by size:
864 493 324 288 281 172 11
Bsu36I CC'TnA_GG
Cuts at: 0 809 2433
Size: 809 1624
BtgI C'CryG_G
Cuts at: 0 441 1298 1502 2249 2433
Size: 441 857 204 747 184
Fragments arranged by size:
857 747 441 204 184
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1604 2433
Size: 1604 829
BtsI GCAGTG_nn'
Cuts at: 0 1171 2028 2433
Size: 1171 857 405
Fragments arranged by size:
1171 857 405
DraIII CAC_nnn'GTG
Cuts at: 0 180 2312 2433
Size: 180 2132 121
Fragments arranged by size:
2132 180 121
EaeI y'GGCC_r
Cuts at: 0 65 665 710 953 963 1301 1837 1881
Size: 65 600 45 243 10 338 536 44
Cuts at: 1881 1934 2296 2433
Size: 53 362 137
Fragments arranged by size:
600 536 362 338 243 137 65 53
45 44 10
EagI C'GGCC_G
Cuts at: 0 1301 1837 2433
Size: 1301 536 596
Fragments arranged by size:
1301 596 536
EarI CTCTTCn'nnn_
Cuts at: 0 323 2433
Size: 323 2110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 930 1849 2202 2256 2344 2433
Size: 930 919 353 54 88 89
Fragments arranged by size:
930 919 353 89 88 54
EcoICRI GAG'CTC
Cuts at: 0 987 2433
Size: 987 1446
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 577 871 910 928 1129 1351 1456
Size: 481 96 294 39 18 201 222 105
Cuts at: 1456 2433
Size: 977
Fragments arranged by size:
977 481 294 222 201 105 96 39
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 2053 2433
Size: 355 1698 380
Fragments arranged by size:
1698 380 355
EcoO109I rG'GnC_Cy
Cuts at: 0 456 831 1461 2198 2199 2433
Size: 456 375 630 737 1 234
Fragments arranged by size:
737 630 456 375 234 1
EcoRI G'AATT_C
Cuts at: 0 2341 2433
Size: 2341 92
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 633 2433
Size: 609 24 1800
Fragments arranged by size:
1800 609 24
FspI TGC'GCA
Cuts at: 0 391 400 997 2433
Size: 391 9 597 1436
Fragments arranged by size:
1436 597 391 9
HaeII r_GCGC'y
Cuts at: 0 90 364 508 916 1016 1116 1442 1799
Size: 90 274 144 408 100 100 326 357
Cuts at: 1799 2433
Size: 634
Fragments arranged by size:
634 408 357 326 274 144 100 100
90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 1117 1149 1641 1673 1783 1815
Size: 273 32 812 32 492 32 110 32
Cuts at: 1815 2433
Size: 618
Fragments arranged by size:
812 618 492 273 110 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2079 2115 2433
Size: 2079 36 318
Fragments arranged by size:
2079 318 36
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 1582 1721 1754 2433
Size: 587 995 139 33 679
Fragments arranged by size:
995 679 587 139 33
MscI TGG'CCA
Cuts at: 0 1883 2433
Size: 1883 550
MslI CAynn'nnrTG
Cuts at: 0 2260 2308 2433
Size: 2260 48 125
Fragments arranged by size:
2260 125 48
MspA1I CmG'CkG
Cuts at: 0 63 387 443 681 873 1224 1273 1504
Size: 63 324 56 238 192 351 49 231
Cuts at: 1504 1548 1722 1842 2088 2433
Size: 44 174 120 246 345
Fragments arranged by size:
351 345 324 246 238 231 192 174
120 63 56 49 44
NaeI GCC'GGC
Cuts at: 0 976 1096 1144 1629 2433
Size: 976 120 48 485 804
Fragments arranged by size:
976 804 485 120 48
NcoI C'CATG_G
Cuts at: 0 2249 2433
Size: 2249 184
NgoMIV G'CCGG_C
Cuts at: 0 974 1094 1142 1627 2433
Size: 974 120 48 485 806
Fragments arranged by size:
974 806 485 120 48
NspI r_CATG'y
Cuts at: 0 2223 2433
Size: 2223 210
PasI CC'CwG_GG
Cuts at: 0 498 2433
Size: 498 1935
PciI A'CATG_T
Cuts at: 0 2219 2433
Size: 2219 214
PflMI CCAn_nnn'nTGG
Cuts at: 0 243 2433
Size: 243 2190
PspOMI G'GGCC_C
Cuts at: 0 1461 1506 2198 2433
Size: 1461 45 692 235
Fragments arranged by size:
1461 692 235 45
PstI C_TGCA'G
Cuts at: 0 272 725 1332 2420 2433
Size: 272 453 607 1088 13
Fragments arranged by size:
1088 607 453 272 13
PvuII CAG'CTG
Cuts at: 0 873 1224 2088 2433
Size: 873 351 864 345
Fragments arranged by size:
873 864 351 345
SacI G_AGCT'C
Cuts at: 0 989 2433
Size: 989 1444
SacII CC_GC'GG
Cuts at: 0 444 1505 2433
Size: 444 1061 928
Fragments arranged by size:
1061 928 444
SalI G'TCGA_C
Cuts at: 0 2077 2433
Size: 2077 356
SapI GCTCTTCn'nnn_
Cuts at: 0 323 2433
Size: 323 2110
SbfI CC_TGCA'GG
Cuts at: 0 1332 2433
Size: 1332 1101
SexAI A'CCwGG_T
Cuts at: 0 1970 2433
Size: 1970 463
SfcI C'TryA_G
Cuts at: 0 189 268 643 721 1328 2416 2433
Size: 189 79 375 78 607 1088 17
Fragments arranged by size:
1088 607 375 189 79 78 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 971 2143 2433
Size: 971 1172 290
Fragments arranged by size:
1172 971 290
SgrAI Cr'CCGG_yG
Cuts at: 0 1627 2257 2433
Size: 1627 630 176
Fragments arranged by size:
1627 630 176
SmaI CCC'GGG
Cuts at: 0 371 1466 2023 2433
Size: 371 1095 557 410
Fragments arranged by size:
1095 557 410 371
SrfI GCCC'GGGC
Cuts at: 0 1466 2433
Size: 1466 967
StuI AGG'CCT
Cuts at: 0 814 1106 2433
Size: 814 292 1327
Fragments arranged by size:
1327 814 292
StyI C'CwwG_G
Cuts at: 0 530 2096 2138 2249 2433
Size: 530 1566 42 111 184
Fragments arranged by size:
1566 530 184 111 42
TatI w'GTAC_w
Cuts at: 0 272 2222 2433
Size: 272 1950 211
Fragments arranged by size:
1950 272 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2209 2241 2433
Size: 2209 32 192
Fragments arranged by size:
2209 192 32
Tth111I GACn'n_nGTC
Cuts at: 0 517 2433
Size: 517 1916
XbaI T'CTAG_A
Cuts at: 0 1065 2433
Size: 1065 1368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1699 2433
Size: 1699 734
XmaI C'CCGG_G
Cuts at: 0 369 1464 2021 2433
Size: 369 1095 557 412
Fragments arranged by size:
1095 557 412 369
XmnI GAAnn'nnTTC
Cuts at: 0 592 2433
Size: 592 1841
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI
PciI PflMI PspOMI PstI PvuII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI SgrAI SmaI SrfI
StuI StyI TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AlfI AloI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BclI BfrBI BglII BmtI BpuEI BsaAI BsaBI BsiWI
BsmI BspEI BspHI BsrGI BstAPI BstBI BstZ17I ClaI
CspCI DraI DrdI EcoRV FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NdeI NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SanDI ScaI
SfoI SgfI SmlI SnaBI SpeI SphI SspI SwaI
TaqII XhoI ZraI