(Circular) (Six-base) MAPSORT of: pFN21ASDA0640.seq Check: 8843 from: 1 to: 6484 pFN21ASDA0640 6484 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4294 278 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3827 1937 Size: 1890 4594 Acc65I G'GTAC_C Cuts at: 3805 3805 Size: 6484 AclI AA'CG_TT Cuts at: 1563 4611 4984 1563 Size: 3048 373 3063 Fragments arranged by size: 3063 3048 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2171 2173 2783 2871 3137 3155 3353 3515 Size: 539 2 610 88 266 18 198 162 Cuts at: 3515 4547 5931 6199 1632 Size: 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1032 610 539 268 266 198 162 88 18 2 AflII C'TTAA_G Cuts at: 820 1017 2450 820 Size: 197 1433 4854 Fragments arranged by size: 4854 1433 197 AflIII A'CryG_T Cuts at: 1203 3294 5383 6360 1203 Size: 2091 2089 977 1327 Fragments arranged by size: 2091 2089 1327 977 AgeI A'CCGG_T Cuts at: 5315 5315 Size: 6484 AhdI GACnn_n'nnGTC Cuts at: 5213 5213 Size: 6484 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6484 AlwNI CAG_nnn'CTG Cuts at: 3085 3508 4227 5799 3085 Size: 423 719 1572 3770 Fragments arranged by size: 3770 1572 719 423 ApaLI G'TGCA_C Cuts at: 2140 4541 5697 2140 Size: 2401 1156 2927 Fragments arranged by size: 2927 2401 1156 ApoI r'AATT_y Cuts at: 1493 2288 3793 4019 4171 6280 1493 Size: 795 1505 226 152 2109 1697 Fragments arranged by size: 2109 1697 1505 795 226 152 AseI AT'TA_AT Cuts at: 160 1032 2417 2622 3653 5038 160 Size: 872 1385 205 1031 1385 1606 Fragments arranged by size: 1606 1385 1385 1031 872 205 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 AvaI C'yCGr_G Cuts at: 1870 1943 3809 1870 Size: 73 1866 4545 Fragments arranged by size: 4545 1866 73 BamHI G'GATC_C Cuts at: 3814 4181 3814 Size: 367 6117 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3805 5260 618 Size: 325 217 627 2018 1455 1842 Fragments arranged by size: 2018 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3803 1391 Size: 2412 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3297 3387 928 Size: 971 1398 90 4025 Fragments arranged by size: 4025 1398 971 90 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4675 4709 1903 Size: 34 2738 34 3678 Fragments arranged by size: 3678 2738 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2421 4376 5586 2421 Size: 1955 1210 3319 Fragments arranged by size: 3319 1955 1210 BclI T'GATC_A Cuts at: 1548 3693 1548 Size: 2145 4339 BfrBI ATG'CAT Cuts at: 2366 2366 Size: 6484 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5095 136 Size: 107 122 71 631 605 3423 1525 Fragments arranged by size: 3423 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6479 6479 Size: 6484 BlpI GC'TnA_GC Cuts at: 3901 3901 Size: 6484 Bme1580I G_kGCm'C Cuts at: 1790 2144 4545 5701 1790 Size: 354 2401 1156 2573 Fragments arranged by size: 2573 2401 1156 354 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6484 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2162 3712 4215 Size: 416 53 83 399 24 745 1550 503 Cuts at: 4215 5173 442 Size: 958 1753 Fragments arranged by size: 1753 1550 958 745 503 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6484 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2801 2801 Size: 6484 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3038 3053 3107 3128 3551 3614 5144 1239 Size: 1799 15 54 21 423 63 1530 2579 Fragments arranged by size: 2579 1799 1530 423 63 54 21 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2102 3144 3560 3584 4609 5474 5772 2102 Size: 1042 416 24 1025 865 298 2814 Fragments arranged by size: 2814 1042 1025 865 416 298 24 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2107 5147 882 Size: 617 608 3040 2219 Fragments arranged by size: 3040 2219 617 608 BsaAI yAC'GTr Cuts at: 493 1204 3781 4275 493 Size: 711 2577 494 2702 Fragments arranged by size: 2702 2577 711 494 BsaBI GATnn'nnATC Cuts at: 6478 6478 Size: 6484 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4291 4673 5308 275 Size: 53 83 186 936 2758 382 635 1451 Fragments arranged by size: 2758 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3000 4917 5315 5589 5736 6147 3000 Size: 1917 398 274 147 411 3337 Fragments arranged by size: 3337 1917 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3377 3407 3527 3557 1178 Size: 30 165 30 1974 30 120 30 4105 Fragments arranged by size: 4105 1974 165 120 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2490 3548 1186 Size: 868 436 1058 4122 Fragments arranged by size: 4122 1058 868 436 BseYI C'CCAG_C Cuts at: 2112 2950 5687 6183 2112 Size: 838 2737 496 2413 Fragments arranged by size: 2737 2413 838 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3147 1163 Size: 573 1411 4500 Fragments arranged by size: 4500 1411 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3934 4695 5723 1540 Size: 275 186 1933 761 1028 2301 Fragments arranged by size: 2301 1933 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 2187 4545 4630 5701 2144 Size: 43 2358 85 1071 2927 Fragments arranged by size: 2927 2358 1071 85 43 BsmI GAATG_Cn' Cuts at: 3995 4088 3995 Size: 93 6391 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2187 3803 4545 4630 5701 1391 Size: 399 354 43 1616 742 85 1071 2174 Fragments arranged by size: 2174 1616 1071 742 399 354 85 43 BspEI T'CCGG_A Cuts at: 3000 6147 3000 Size: 3147 3337 BspHI T'CATG_A Cuts at: 2161 3643 4373 6103 2161 Size: 1482 730 1730 2542 Fragments arranged by size: 2542 1730 1482 730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2454 3718 3839 844 Size: 1610 1264 121 3489 Fragments arranged by size: 3489 1610 1264 121 BsrBI CCG'CTC Cuts at: 1394 1823 4371 1394 Size: 429 2548 3507 Fragments arranged by size: 3507 2548 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4979 5153 65 Size: 1183 3731 174 1396 Fragments arranged by size: 3731 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2598 5128 5315 1700 Size: 110 788 2530 187 2869 Fragments arranged by size: 2869 2530 788 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6484 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6484 BssSI C'ACGA_G Cuts at: 3123 4544 5556 3123 Size: 1421 1012 4051 Fragments arranged by size: 4051 1421 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3125 794 Size: 2331 4153 BstBI TT'CG_AA Cuts at: 2007 5288 2007 Size: 3281 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6484 BstXI CCAn_nnnn'nTGG Cuts at: 3382 3382 Size: 6484 BstYI r'GATC_y Cuts at: 1972 2758 3814 4181 4566 4583 6035 6121 6133 Size: 786 1056 367 385 17 1452 86 12 Cuts at: 6133 6479 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1056 786 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3519 3771 513 Size: 552 957 1497 252 3226 Fragments arranged by size: 3226 1497 957 552 252 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5745 Fragments arranged by size: 5745 683 56 BtsI GCAGTG_nn' Cuts at: 4000 4794 4814 4000 Size: 794 20 5670 Fragments arranged by size: 5670 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6484 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6449 DraI TTT'AAA Cuts at: 2241 2371 3788 2241 Size: 130 1417 4937 Fragments arranged by size: 4937 1417 130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5491 809 Size: 507 4175 1802 Fragments arranged by size: 4175 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3931 4820 6449 Size: 54 1425 50 275 18 2101 889 1629 Cuts at: 6449 8 Size: 43 Fragments arranged by size: 2101 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3931 1537 Size: 275 2119 4090 Fragments arranged by size: 4090 2119 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2175 2427 3129 3345 3384 3732 4414 1353 Size: 822 252 702 216 39 348 682 3423 Fragments arranged by size: 3423 822 702 682 348 252 216 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3476 5071 5445 5591 387 Size: 1064 138 1887 1595 374 146 1280 Fragments arranged by size: 1887 1595 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2173 2783 2871 3038 3053 3107 Size: 393 539 2 610 88 167 15 54 Cuts at: 3107 3128 3137 3155 3353 3515 3551 3614 4547 Size: 21 9 18 198 162 36 63 933 Cuts at: 4547 5144 5931 6199 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 933 787 610 597 539 393 268 198 167 162 88 63 54 36 21 18 15 9 2 EcoNI CCTnn'n_nnAGG Cuts at: 3512 3512 Size: 6484 EcoO109I rG'GnC_Cy Cuts at: 2122 3505 3707 2122 Size: 1383 202 4899 Fragments arranged by size: 4899 1383 202 EcoRI G'AATT_C Cuts at: 2288 3793 4171 2288 Size: 1505 378 4601 Fragments arranged by size: 4601 1505 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3185 3442 3185 Size: 257 6227 FspI TGC'GCA Cuts at: 4990 4990 Size: 6484 HaeII r_GCGC'y Cuts at: 1678 5631 1678 Size: 3953 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2743 2775 3377 3409 5125 Size: 32 135 32 1171 32 602 32 1716 Cuts at: 5125 5157 5199 5231 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1716 1171 602 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3828 4074 669 Size: 988 281 1890 246 3079 Fragments arranged by size: 3079 1890 988 281 246 HindIII A'AGCT_T Cuts at: 748 2456 3206 3365 748 Size: 1708 750 159 3867 Fragments arranged by size: 3867 1708 750 159 HpaI GTT'AAC Cuts at: 4074 4074 Size: 6484 KpnI G_GTAC'C Cuts at: 3809 3809 Size: 6484 MfeI C'AATT_G Cuts at: 4083 4083 Size: 6484 MluI A'CGCG_T Cuts at: 6360 6360 Size: 6484 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2241 2285 2969 3363 3456 5598 5782 1305 Size: 936 44 684 394 93 2142 184 2007 Fragments arranged by size: 2142 2007 936 684 394 184 93 44 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4662 Fragments arranged by size: 4662 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4442 4801 4960 518 Size: 648 3276 359 159 2042 Fragments arranged by size: 3276 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 3013 3529 3900 4577 5725 5970 1606 Size: 488 919 516 371 677 1148 245 2120 Fragments arranged by size: 2120 1148 919 677 516 488 371 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3519 513 Size: 552 957 1497 3478 Fragments arranged by size: 3478 1497 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6484 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6484 NotI GC'GGCC_GC Cuts at: 3931 3931 Size: 6484 NsiI A_TGCA'T Cuts at: 2368 2368 Size: 6484 NspI r_CATG'y Cuts at: 1132 1229 2233 3298 3842 5387 1132 Size: 97 1004 1065 544 1545 2229 Fragments arranged by size: 2229 1545 1065 1004 544 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6484 PciI A'CATG_T Cuts at: 3294 5383 3294 Size: 2089 4395 PflMI CCAn_nnn'nTGG Cuts at: 1108 3621 1108 Size: 2513 3971 PmeI GTTT'AAAC Cuts at: 3788 3788 Size: 6484 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4553 4585 6066 6098 4553 Size: 32 1481 32 4939 Fragments arranged by size: 4939 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2122 3707 2122 Size: 1585 4899 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6484 PsiI TTA'TAA Cuts at: 2508 4054 2508 Size: 1546 4938 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3252 3284 3252 Size: 32 6452 PstI C_TGCA'G Cuts at: 830 3312 3513 3715 3836 830 Size: 2482 201 202 121 3478 Fragments arranged by size: 3478 2482 202 201 121 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6484 PvuII CAG'CTG Cuts at: 3013 3529 3013 Size: 516 5968 SalI G'TCGA_C Cuts at: 1936 3826 1936 Size: 1890 4594 SapI GCTCTTCn'nnn_ Cuts at: 2175 2175 Size: 6484 SbfI CC_TGCA'GG Cuts at: 3513 3836 3513 Size: 323 6161 ScaI AGT'ACT Cuts at: 4732 6444 4732 Size: 1712 4772 SexAI A'CCwGG_T Cuts at: 3670 3670 Size: 6484 SfcI C'TryA_G Cuts at: 826 1045 3308 3509 3711 3832 4967 5648 5839 Size: 219 2263 201 202 121 1135 681 191 Cuts at: 5839 826 Size: 1471 Fragments arranged by size: 2263 1471 1135 681 219 202 201 191 121 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6484 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6484 SmaI CCC'GGG Cuts at: 1872 3811 1872 Size: 1939 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 2450 3159 3539 3563 4588 Size: 197 926 174 333 709 380 24 1025 Cuts at: 4588 5489 5751 820 Size: 901 262 1553 Fragments arranged by size: 1553 1025 926 901 709 380 333 262 197 174 24 SnaBI TAC'GTA Cuts at: 493 3781 4275 493 Size: 3288 494 2702 Fragments arranged by size: 3288 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6484 SphI G_CATG'C Cuts at: 1132 3842 1132 Size: 2710 3774 SspI AAT'ATT Cuts at: 5 52 4408 5 Size: 47 4356 2081 Fragments arranged by size: 4356 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6484 StyI C'CwwG_G Cuts at: 513 1065 2022 3519 3923 513 Size: 552 957 1497 404 3074 Fragments arranged by size: 3074 1497 957 552 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4511 4528 4681 4866 5311 325 Size: 1255 2931 17 153 185 445 1498 Fragments arranged by size: 2931 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2719 4730 6442 Size: 274 80 33 51 1443 742 2011 1712 Cuts at: 6442 96 Size: 138 Fragments arranged by size: 2011 1712 1443 742 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2299 2323 2331 2355 6444 6476 2299 Size: 24 8 24 4089 32 2307 Fragments arranged by size: 4089 2307 32 24 24 8 Tth111I GACn'n_nGTC Cuts at: 1316 2608 1316 Size: 1292 5192 XbaI T'CTAG_A Cuts at: 3820 3820 Size: 6484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3526 3526 Size: 6484 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6484 XmaI C'CCGG_G Cuts at: 1870 3809 1870 Size: 1939 4545 XmnI GAAnn'nnTTC Cuts at: 4613 4613 Size: 6484 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4292 276 Size: 53 83 186 936 2758 2468 Fragments arranged by size: 2758 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI