Restriction map for
pFN21ASDA0636
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI AvaI AviII AvrII
BamHI BanI BanII BbeI BbvCI
BcgI BciVI BcnI BfaI BfrI
BlnI BlpI BmyI Bpu1102I BsaBI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsoBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRV FauI FseI
FspI HapII HgaI Hin1I HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MspI MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 1422 CCGC CCGC(-3/-1)
AfaI 299 GTAC GT^AC
AhdI 910 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 459 GGATC GGATC(4/5)
Asp700I 208 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 910 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 1177 GGACC G^G[AT]CC
BbrPI 1207 CACGTG CAC^GTG
BbsI 1154 GTCTTC GAAGAC(2/6)
BclI 1289 TGATCA T^GATCA
BglI 944 GCCCCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 584 AGATCT A^GATCT
BpuAI 1154 GTCTTC GAAGAC(2/6)
BsaAI 1207 CACGTG [CT]AC^GT[GA]
BsaI 917 GGTCTC GGTCTC(1/5)
BseRI 1055 CTCCTC GAGGAG(10/8)
BsmFI 987 GGGAC GGGAC(10/14)
BsrBI 1422 CCGCTC CCGCTC(-3/-3)
BsrDI 1115 GCAATG GCAATG(2/0)
BstEII 907 GGTGACC G^GT[ACGT]ACC
BstPI 907 GGTGACC G^GT[ACGT]ACC
Cfr13I 1177 GGACC G^G[ACGT]CC
DsaI 1388 CCATGG C^C[GA][CT]GG
Eam1105I 910 GACCCAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 907 GGTGACC G^GT[ACGT]ACC
EcoT22I 744 ATGCAT ATGCA^T
FbaI 1289 TGATCA T^GATCA
HincII 116 GTCAAC GT[CT]^[GA]AC
HindII 116 GTCAAC GT[CT]^[GA]AC
MaeII 1208 ACGT A^CGT
NcoI 1388 CCATGG C^CATGG
NlaIV 898 GGCTCC GG[ACGT]^[ACGT]CC
NsiI 744 ATGCAT ATGCA^T
PleI 925 GACTC GAGTC(4/5)
PmaCI 1207 CACGTG CAC^GTG
PmlI 1207 CACGTG CAC^GTG
RsaI 299 GTAC GT^AC
Sau96I 1177 GGACC G^G[ACGT]CC
SbfI 364 CCTGCAGG CCTGCA^GG
SfaNI 1009 GATGC GCATC(5/9)
Sse8387I 364 CCTGCAGG CCTGCA^GG
SspI 1256 AATATT AAT^ATT
StuI 574 AGGCCT AGG^CCT
XcmI 1388 CCATGGAGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 208 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
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BalI 1211 TGGCCA TGG^CCA
1442 TGGCCA
BsmAI 918 GTCTC GTCTC(1/5)
1418 GTCTC
BstYI 459 GGATCT [GA]^GATC[CT]
584 AGATCT
EaeI 1211 TGGCCA [CT]^GGCC[GA]
1442 TGGCCA
EcoRI 490 GAATTC G^AATTC
786 GAATTC
MboII 397 GAAGA GAAGA(8/7)
1155 TCTTC
MflI 459 GGATCT [GA]^GATC[CT]
584 AGATCT
MluNI 1211 TGGCCA TGG^CCA
1442 TGGCCA
MscI 1211 TGGCCA TGG^CCA
1442 TGGCCA
MslI 18 CACAAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
627 CATTAAGGTG
MspA1I 392 CAGCTG C[AC]G^C[GT]G
865 CAGCTG
PflMI 1027 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAGCAGGTGG
PstI 365 CTGCAG CTGCA^G
1226 CTGCAG
PvuII 392 CAGCTG CAG^CTG
865 CAGCTG
TaqI 245 TCGA T^CGA
336 TCGA
TthHB8I 245 TCGA T^CGA
336 TCGA
Van91I 1027 CCAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1536 CCAGCAGGTGG
XhoII 459 GGATCT [GA]^GATC[CT]
584 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AluI ApoI BbvI BpmI
BsaJI BsiYI BslI BsmI BspMI
BsrI BssKI BstF5I BstNI BstXI
CfoI DdeI DpnII EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI MaeIII
MboI MnlI MseI MvaI MwoI
NdeII NlaIII Sau3AI ScrFI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI