(Circular) (Six-base) MAPSORT of: pFN21ASDA0636.seq Check: 8827 from: 1 to: 6340 pFN21ASDA0636 6340 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3553 3553 Size: 6340 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4150 278 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3683 1937 Size: 1746 4594 Acc65I G'GTAC_C Cuts at: 3661 3661 Size: 6340 AclI AA'CG_TT Cuts at: 1563 4467 4840 1563 Size: 2904 373 3063 Fragments arranged by size: 3063 2904 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2439 3388 4403 5787 6055 1632 Size: 807 949 1015 1384 268 1917 Fragments arranged by size: 1917 1384 1015 949 807 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6143 AflIII A'CryG_T Cuts at: 1203 5239 6216 1203 Size: 4036 977 1327 Fragments arranged by size: 4036 1327 977 AgeI A'CCGG_T Cuts at: 5171 5171 Size: 6340 AhdI GACnn_n'nnGTC Cuts at: 2938 5069 2938 Size: 2131 4209 AleI CACnn'nnGTG Cuts at: 1166 2045 1166 Size: 879 5461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6340 AlwNI CAG_nnn'CTG Cuts at: 4083 5655 4083 Size: 1572 4768 ApaLI G'TGCA_C Cuts at: 4397 5553 4397 Size: 1156 5184 ApoI r'AATT_y Cuts at: 1493 2249 2513 2759 2809 2893 3101 3649 3875 Size: 756 264 246 50 84 208 548 226 Cuts at: 3875 4027 6136 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 756 548 264 246 226 208 152 84 50 AseI AT'TA_AT Cuts at: 160 1032 4894 160 Size: 872 3862 1606 Fragments arranged by size: 3862 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 AvaI C'yCGr_G Cuts at: 1870 1943 3665 1870 Size: 73 1722 4545 Fragments arranged by size: 4545 1722 73 BamHI G'GATC_C Cuts at: 3670 4037 3670 Size: 367 5973 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3661 5116 618 Size: 325 217 627 1874 1455 1842 Fragments arranged by size: 1874 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3659 1391 Size: 2268 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3170 928 Size: 971 1271 4098 Fragments arranged by size: 4098 1271 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4531 4565 1903 Size: 34 2594 34 3678 Fragments arranged by size: 3678 2594 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4232 5442 4232 Size: 1210 5130 BclI T'GATC_A Cuts at: 1548 3312 1548 Size: 1764 4576 BfrBI ATG'CAT Cuts at: 2769 2769 Size: 6340 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2973 4951 136 Size: 107 122 71 631 605 1301 1978 1525 Fragments arranged by size: 1978 1525 1301 631 605 122 107 71 BglII A'GATC_T Cuts at: 2607 6335 2607 Size: 3728 2612 BlpI GC'TnA_GC Cuts at: 3757 3757 Size: 6340 Bme1580I G_kGCm'C Cuts at: 1790 4401 5557 1790 Size: 2611 1156 2573 Fragments arranged by size: 2611 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6340 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 4071 5029 Size: 416 53 83 399 24 609 2045 958 Cuts at: 5029 442 Size: 1753 Fragments arranged by size: 2045 1753 958 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2725 2928 3340 5000 1239 Size: 1486 203 412 1660 2579 Fragments arranged by size: 2579 1660 1486 412 203 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4465 5330 5628 4465 Size: 865 298 5177 Fragments arranged by size: 5177 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2946 5003 882 Size: 617 1447 2057 2219 Fragments arranged by size: 2219 2057 1447 617 BsaAI yAC'GTr Cuts at: 493 1204 3232 3637 4131 493 Size: 711 2028 405 494 2702 Fragments arranged by size: 2702 2028 711 494 405 BsaBI GATnn'nnATC Cuts at: 6334 6334 Size: 6340 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4147 4529 5164 275 Size: 53 83 186 936 2614 382 635 1451 Fragments arranged by size: 2614 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4773 5171 5445 5592 6003 4773 Size: 398 274 147 411 5110 Fragments arranged by size: 5110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6115 Fragments arranged by size: 6115 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3069 1186 Size: 1883 4457 BseYI C'CCAG_C Cuts at: 3558 3610 5543 6039 3558 Size: 52 1933 496 3859 Fragments arranged by size: 3859 1933 496 52 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5767 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3790 4551 5579 1540 Size: 275 186 1789 761 1028 2301 Fragments arranged by size: 2301 1789 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4401 4486 5557 4401 Size: 85 1071 5184 Fragments arranged by size: 5184 1071 85 BsmI GAATG_Cn' Cuts at: 2771 2858 3517 3851 3944 2771 Size: 87 659 334 93 5167 Fragments arranged by size: 5167 659 334 93 87 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3659 4401 4486 5557 1391 Size: 399 1869 742 85 1071 2174 Fragments arranged by size: 2174 1869 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6003 6003 Size: 6340 BspHI T'CATG_A Cuts at: 4229 5959 4229 Size: 1730 4610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2395 3079 3553 3695 844 Size: 1551 684 474 142 3489 Fragments arranged by size: 3489 1551 684 474 142 BsrBI CCG'CTC Cuts at: 1394 1823 3447 4227 1394 Size: 429 1624 780 3507 Fragments arranged by size: 3507 1624 780 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3145 4835 5009 65 Size: 1183 1897 1690 174 1396 Fragments arranged by size: 1897 1690 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4984 5171 1700 Size: 110 3174 187 2869 Fragments arranged by size: 3174 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6340 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6340 BssSI C'ACGA_G Cuts at: 4400 5412 4400 Size: 1012 5328 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6340 BstBI TT'CG_AA Cuts at: 2007 5144 2007 Size: 3137 3203 BstEII G'GTnAC_C Cuts at: 1184 2930 1184 Size: 1746 4594 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2470 2656 2077 Size: 393 186 5761 Fragments arranged by size: 5761 393 186 BstYI r'GATC_y Cuts at: 1972 2482 2607 3670 4037 4422 4439 5891 5977 Size: 510 125 1063 367 385 17 1452 86 Cuts at: 5977 5989 6335 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1063 510 385 367 346 125 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3411 513 Size: 552 957 1389 3442 Fragments arranged by size: 3442 1389 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5601 Fragments arranged by size: 5601 683 56 BtsI GCAGTG_nn' Cuts at: 3856 4650 4670 3856 Size: 794 20 5526 Fragments arranged by size: 5526 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6340 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6305 DraI TTT'AAA Cuts at: 3644 3644 Size: 6340 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5347 809 Size: 507 4031 1802 Fragments arranged by size: 4031 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3234 3465 3787 Size: 54 1425 50 275 18 1404 231 322 Cuts at: 3787 4676 6305 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1404 889 322 275 231 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3787 1537 Size: 275 1975 4090 Fragments arranged by size: 4090 1975 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4270 1353 Size: 2917 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4927 5301 5447 387 Size: 1064 138 3338 374 146 1280 Fragments arranged by size: 3338 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2439 2725 2928 3340 3388 4403 5000 Size: 393 807 286 203 412 48 1015 597 Cuts at: 5000 5787 6055 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1015 807 787 597 412 393 286 268 203 48 EcoRI G'AATT_C Cuts at: 2513 2809 3649 4027 2513 Size: 296 840 378 4826 Fragments arranged by size: 4826 840 378 296 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2200 3545 2200 Size: 1345 4995 FspI TGC'GCA Cuts at: 4846 4846 Size: 6340 HaeII r_GCGC'y Cuts at: 1678 2054 3069 3411 5487 1678 Size: 376 1015 342 2076 2531 Fragments arranged by size: 2531 2076 1015 376 342 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2924 2956 3064 Size: 32 135 32 506 32 814 32 108 Cuts at: 3064 3096 3259 3291 3465 3497 4981 5013 5055 Size: 32 163 32 174 32 1484 32 42 Cuts at: 5055 5087 1373 Size: 32 2626 Fragments arranged by size: 2626 1484 814 506 174 163 135 108 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2141 3684 3930 669 Size: 988 281 203 1543 246 3079 Fragments arranged by size: 3079 1543 988 281 246 203 HindIII A'AGCT_T Cuts at: 748 748 Size: 6340 HpaI GTT'AAC Cuts at: 3930 3930 Size: 6340 KpnI G_GTAC'C Cuts at: 3665 3665 Size: 6340 MfeI C'AATT_G Cuts at: 3939 3939 Size: 6340 MluI A'CGCG_T Cuts at: 6216 6216 Size: 6340 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3364 3370 5454 5638 1305 Size: 2059 6 2084 184 2007 Fragments arranged by size: 2084 2059 2007 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 3236 3467 10 Size: 54 1425 343 1404 231 2883 Fragments arranged by size: 2883 1425 1404 343 231 54 MslI CAynn'nnrTG Cuts at: 518 1166 2045 2654 4298 4657 4816 518 Size: 648 879 609 1644 359 159 2042 Fragments arranged by size: 2042 1644 879 648 609 359 159 MspA1I CmG'CkG Cuts at: 1606 2417 2890 3756 4433 5581 5826 1606 Size: 811 473 866 677 1148 245 2120 Fragments arranged by size: 2120 1148 866 811 677 473 245 NcoI C'CATG_G Cuts at: 513 1065 2022 3411 513 Size: 552 957 1389 3442 Fragments arranged by size: 3442 1389 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6340 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6340 NotI GC'GGCC_GC Cuts at: 3787 3787 Size: 6340 NsiI A_TGCA'T Cuts at: 2771 2771 Size: 6340 NspI r_CATG'y Cuts at: 1132 1229 3698 5243 1132 Size: 97 2469 1545 2229 Fragments arranged by size: 2469 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6340 PciI A'CATG_T Cuts at: 5239 5239 Size: 6340 PflMI CCAn_nnn'nTGG Cuts at: 1108 3056 3565 1108 Size: 1948 509 3883 Fragments arranged by size: 3883 1948 509 PmeI GTTT'AAAC Cuts at: 3644 3644 Size: 6340 PmlI CAC'GTG Cuts at: 3232 3232 Size: 6340 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4409 4441 5922 5954 4409 Size: 32 1481 32 4795 Fragments arranged by size: 4795 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6340 PsiI TTA'TAA Cuts at: 3910 3910 Size: 6340 PstI C_TGCA'G Cuts at: 830 2392 3253 3692 830 Size: 1562 861 439 3478 Fragments arranged by size: 3478 1562 861 439 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6340 PvuII CAG'CTG Cuts at: 2417 2890 2417 Size: 473 5867 SalI G'TCGA_C Cuts at: 1936 3682 1936 Size: 1746 4594 SbfI CC_TGCA'GG Cuts at: 2392 3692 2392 Size: 1300 5040 ScaI AGT'ACT Cuts at: 4588 6300 4588 Size: 1712 4628 SfcI C'TryA_G Cuts at: 826 1045 2388 3249 3342 3688 4823 5504 5695 Size: 219 1343 861 93 346 1135 681 191 Cuts at: 5695 826 Size: 1471 Fragments arranged by size: 1471 1343 1135 861 681 346 219 191 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6340 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6340 SmaI CCC'GGG Cuts at: 1872 3667 1872 Size: 1795 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4444 5345 5607 820 Size: 197 926 2501 901 262 1553 Fragments arranged by size: 2501 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3637 4131 493 Size: 3144 494 2702 Fragments arranged by size: 3144 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6340 SphI G_CATG'C Cuts at: 1132 3698 1132 Size: 2566 3774 SspI AAT'ATT Cuts at: 5 52 3281 4264 5 Size: 47 3229 983 2081 Fragments arranged by size: 3229 2081 983 47 StuI AGG'CCT Cuts at: 1517 2599 1517 Size: 1082 5258 StyI C'CwwG_G Cuts at: 513 1065 2022 2326 2936 3148 3411 3779 513 Size: 552 957 304 610 212 263 368 3074 Fragments arranged by size: 3074 957 610 552 368 304 263 212 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3159 4367 4384 4537 4722 5167 325 Size: 1255 1579 1208 17 153 185 445 1498 Fragments arranged by size: 1579 1498 1255 1208 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4586 6298 96 Size: 274 80 33 51 1443 2609 1712 138 Fragments arranged by size: 2609 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6300 6332 6300 Size: 32 6308 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6340 XbaI T'CTAG_A Cuts at: 3676 3676 Size: 6340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3418 3418 Size: 6340 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6340 XmaI C'CCGG_G Cuts at: 1870 3665 1870 Size: 1795 4545 XmnI GAAnn'nnTTC Cuts at: 2235 4469 2235 Size: 2234 4106 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4148 276 Size: 53 83 186 936 2614 2468 Fragments arranged by size: 2614 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI