Restriction map for
pFN21ASDA0636
(Linear) (Six-base) MAPSORT of: KIAA0636.seq Check: 6025 from: 1 to: 1611
KIAA0636.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1530 1611
Size: 1530 81
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 1365 1611
Size: 416 949 246
Fragments arranged by size:
949 416 246
AhdI GACnn_n'nnGTC
Cuts at: 0 915 1611
Size: 915 696
AleI CACnn'nnGTG
Cuts at: 0 22 1611
Size: 22 1589
ApoI r'AATT_y
Cuts at: 0 226 490 736 786 870 1078 1611
Size: 226 264 246 50 84 208 533
Fragments arranged by size:
533 264 246 226 208 84 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 1147 1611
Size: 1147 464
BclI T'GATC_A
Cuts at: 0 1289 1611
Size: 1289 322
BfrBI ATG'CAT
Cuts at: 0 746 1611
Size: 746 865
BglI GCCn_nnn'nGGC
Cuts at: 0 950 1611
Size: 950 661
BglII A'GATC_T
Cuts at: 0 584 1611
Size: 584 1027
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 1611
Size: 3 1608
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 702 905 1317 1611
Size: 702 203 412 294
Fragments arranged by size:
702 412 294 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 923 1611
Size: 923 688
BsaAI yAC'GTr
Cuts at: 0 1209 1611
Size: 1209 402
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1046 1611
Size: 1046 565
BseYI C'CCAG_C
Cuts at: 0 1535 1587 1611
Size: 1535 52 24
Fragments arranged by size:
1535 52 24
BsmI GAATG_Cn'
Cuts at: 0 748 835 1494 1611
Size: 748 87 659 117
Fragments arranged by size:
748 659 117 87
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 372 1056 1530 1611
Size: 372 684 474 81
Fragments arranged by size:
684 474 372 81
BsrBI CCG'CTC
Cuts at: 0 1424 1611
Size: 1424 187
BsrDI GCAATG_nn'
Cuts at: 0 1122 1611
Size: 1122 489
BstEII G'GTnAC_C
Cuts at: 0 907 1611
Size: 907 704
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 447 633 1611
Size: 54 393 186 978
Fragments arranged by size:
978 393 186 54
BstYI r'GATC_y
Cuts at: 0 459 584 1611
Size: 459 125 1027
Fragments arranged by size:
1027 459 125
BtgI C'CryG_G
Cuts at: 0 1388 1611
Size: 1388 223
EaeI y'GGCC_r
Cuts at: 0 1211 1442 1611
Size: 1211 231 169
Fragments arranged by size:
1211 231 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 416 702 905 1317 1365 1611
Size: 416 286 203 412 48 246
Fragments arranged by size:
416 412 286 246 203 48
EcoRI G'AATT_C
Cuts at: 0 490 786 1611
Size: 490 296 825
Fragments arranged by size:
825 490 296
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 177 1522 1611
Size: 177 1345 89
Fragments arranged by size:
1345 177 89
HaeII r_GCGC'y
Cuts at: 0 31 1046 1388 1611
Size: 31 1015 342 223
Fragments arranged by size:
1015 342 223 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 901 933 1041 1073 1236 1268
Size: 55 32 814 32 108 32 163 32
Cuts at: 1268 1442 1474 1611
Size: 174 32 137
Fragments arranged by size:
814 174 163 137 108 55 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 118 1611
Size: 118 1493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1341 1347 1611
Size: 1341 6 264
Fragments arranged by size:
1341 264 6
MscI TGG'CCA
Cuts at: 0 1213 1444 1611
Size: 1213 231 167
Fragments arranged by size:
1213 231 167
MslI CAynn'nnrTG
Cuts at: 0 22 631 1611
Size: 22 609 980
Fragments arranged by size:
980 609 22
MspA1I CmG'CkG
Cuts at: 0 394 867 1611
Size: 394 473 744
Fragments arranged by size:
744 473 394
NcoI C'CATG_G
Cuts at: 0 1388 1611
Size: 1388 223
NsiI A_TGCA'T
Cuts at: 0 748 1611
Size: 748 863
PflMI CCAn_nnn'nTGG
Cuts at: 0 1033 1542 1611
Size: 1033 509 69
Fragments arranged by size:
1033 509 69
PmlI CAC'GTG
Cuts at: 0 1209 1611
Size: 1209 402
PstI C_TGCA'G
Cuts at: 0 369 1230 1611
Size: 369 861 381
Fragments arranged by size:
861 381 369
PvuII CAG'CTG
Cuts at: 0 394 867 1611
Size: 394 473 744
Fragments arranged by size:
744 473 394
SbfI CC_TGCA'GG
Cuts at: 0 369 1611
Size: 369 1242
SfcI C'TryA_G
Cuts at: 0 365 1226 1319 1611
Size: 365 861 93 292
Fragments arranged by size:
861 365 292 93
SspI AAT'ATT
Cuts at: 0 1258 1611
Size: 1258 353
StuI AGG'CCT
Cuts at: 0 576 1611
Size: 576 1035
StyI C'CwwG_G
Cuts at: 0 303 913 1125 1388 1611
Size: 303 610 212 263 223
Fragments arranged by size:
610 303 263 223 212
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1136 1611
Size: 1136 475
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1395 1611
Size: 1395 216
XmnI GAAnn'nnTTC
Cuts at: 0 212 1611
Size: 212 1399
Enzymes that do cut:
AarI AcuI AhdI AleI ApoI BbsI BclI BfrBI
BglI BglII BmrI BpmI BsaI BsaAI BseRI BseYI
BsmI BspMI BsrBI BsrDI BstEII BstXI BstYI BtgI
EaeI Eco57MI EcoRI FalI HaeII Hin4I HincII MmeI
MscI MslI MspA1I NcoI NsiI PflMI PmlI PstI
PvuII SbfI SfcI SspI StuI StyI TaqII XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BlpI Bme1580I BmgBI BmtI BplI Bpu10I
BpuEI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TatI TstI Tth111I XbaI
XhoI XmaI ZraI