(Circular) (Six-base) MAPSORT of: pFN21ASDA0632.seq Check: 7571 from: 1 to: 6829 pFN21ASDA0632 6829 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4639 278 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3736 4172 1937 Size: 1799 436 4594 Fragments arranged by size: 4594 1799 436 Acc65I G'GTAC_C Cuts at: 3942 4150 3942 Size: 208 6621 AclI AA'CG_TT Cuts at: 1563 4956 5329 1563 Size: 3393 373 3063 Fragments arranged by size: 3393 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2143 2534 2609 2738 2804 2860 3068 3077 Size: 511 391 75 129 66 56 208 9 Cuts at: 3077 3335 4892 6276 6544 1632 Size: 258 1557 1384 268 1917 Fragments arranged by size: 1917 1557 1384 511 391 268 258 208 129 75 66 56 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6632 AflIII A'CryG_T Cuts at: 1203 5728 6705 1203 Size: 4525 977 1327 Fragments arranged by size: 4525 1327 977 AgeI A'CCGG_T Cuts at: 3939 5660 3939 Size: 1721 5108 AhdI GACnn_n'nnGTC Cuts at: 5558 5558 Size: 6829 AleI CACnn'nnGTG Cuts at: 1166 3305 3599 1166 Size: 2139 294 4396 Fragments arranged by size: 4396 2139 294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6829 AlwNI CAG_nnn'CTG Cuts at: 2512 2734 4006 4572 6144 2512 Size: 222 1272 566 1572 3197 Fragments arranged by size: 3197 1572 1272 566 222 ApaI G_GGCC'C Cuts at: 2062 2062 Size: 6829 ApaLI G'TGCA_C Cuts at: 4886 6042 4886 Size: 1156 5673 ApoI r'AATT_y Cuts at: 1493 4138 4364 4516 6625 1493 Size: 2645 226 152 2109 1697 Fragments arranged by size: 2645 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5383 160 Size: 872 4351 1606 Fragments arranged by size: 4351 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 AvaI C'yCGr_G Cuts at: 1870 1943 2230 3009 3356 4154 1870 Size: 73 287 779 347 798 4545 Fragments arranged by size: 4545 798 779 347 287 73 AvrII C'CTAG_G Cuts at: 3022 3022 Size: 6829 BamHI G'GATC_C Cuts at: 4159 4526 4159 Size: 367 6462 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2152 3714 3942 4150 5605 Size: 325 217 627 365 1562 228 208 1455 Cuts at: 5605 618 Size: 1842 Fragments arranged by size: 1842 1562 1455 627 365 325 228 217 208 BanII G_rGCy'C Cuts at: 1391 2062 2225 2400 2640 2757 3282 4148 1391 Size: 671 163 175 240 117 525 866 4072 Fragments arranged by size: 4072 866 671 525 240 175 163 117 BbeI G_GCGC'C Cuts at: 2156 2156 Size: 6829 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2271 2918 928 Size: 971 372 647 4839 Fragments arranged by size: 4839 971 647 372 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5020 5054 1903 Size: 34 3083 34 3678 Fragments arranged by size: 3678 3083 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3259 4721 5931 3259 Size: 1462 1210 4157 Fragments arranged by size: 4157 1462 1210 BclI T'GATC_A Cuts at: 1548 2460 1548 Size: 912 5917 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2114 3438 5440 Size: 107 122 71 631 605 442 1324 2002 Cuts at: 5440 136 Size: 1525 Fragments arranged by size: 2002 1525 1324 631 605 442 122 107 71 BglII A'GATC_T Cuts at: 6824 6824 Size: 6829 BlpI GC'TnA_GC Cuts at: 4246 4246 Size: 6829 Bme1580I G_kGCm'C Cuts at: 1790 2062 2856 3243 3799 4111 4890 6046 1790 Size: 272 794 387 556 312 779 1156 2573 Fragments arranged by size: 2573 1156 794 779 556 387 312 272 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2338 3086 3913 Size: 416 53 83 399 24 921 748 827 Cuts at: 3913 4560 5518 442 Size: 647 958 1753 Fragments arranged by size: 1753 958 921 827 748 647 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6829 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2058 2058 Size: 6829 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2654 2918 3194 3201 3455 3650 3926 3986 Size: 1415 264 276 7 254 195 276 60 Cuts at: 3986 5489 1239 Size: 1503 2579 Fragments arranged by size: 2579 1503 1415 276 276 264 254 195 60 7 Bpu10I CC'TnA_GC Cuts at: 3446 3446 Size: 6829 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4954 5819 6117 4954 Size: 865 298 5666 Fragments arranged by size: 5666 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2864 3385 3618 5492 882 Size: 617 1365 521 233 1874 2219 Fragments arranged by size: 2219 1874 1365 617 521 233 BsaAI yAC'GTr Cuts at: 493 1204 2857 4126 4620 493 Size: 711 1653 1269 494 2702 Fragments arranged by size: 2702 1653 1269 711 494 BsaBI GATnn'nnATC Cuts at: 6823 6823 Size: 6829 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2153 2646 4636 5018 Size: 53 83 186 936 620 493 1990 382 Cuts at: 5018 5653 275 Size: 635 1451 Fragments arranged by size: 1990 1451 936 635 620 493 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2392 2922 3226 3939 4053 5262 5660 5934 6081 Size: 530 304 713 114 1209 398 274 147 Cuts at: 6081 6492 2392 Size: 411 2729 Fragments arranged by size: 2729 1209 713 530 411 398 304 274 147 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 2294 2324 1178 Size: 30 165 30 825 30 36 30 5683 Fragments arranged by size: 5683 825 165 36 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2158 2161 2285 2288 2291 2294 2302 2534 Size: 972 3 124 3 3 3 8 232 Cuts at: 2534 2882 3083 1186 Size: 348 201 4932 Fragments arranged by size: 4932 972 348 232 201 124 8 3 3 3 3 BseYI C'CCAG_C Cuts at: 3281 3403 3619 6032 6528 3281 Size: 122 216 2413 496 3582 Fragments arranged by size: 3582 2413 496 216 122 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6256 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4279 5040 6068 1540 Size: 275 186 2278 761 1028 2301 Fragments arranged by size: 2301 2278 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2757 3465 3564 4890 4975 6046 2757 Size: 708 99 1326 85 1071 3540 Fragments arranged by size: 3540 1326 1071 708 99 85 BsmI GAATG_Cn' Cuts at: 4340 4433 4340 Size: 93 6736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2225 2400 2640 2757 2856 3243 Size: 399 272 163 175 240 117 99 387 Cuts at: 3243 3282 3465 3564 3799 4111 4148 4890 4975 Size: 39 183 99 235 312 37 742 85 Cuts at: 4975 6046 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 312 272 240 235 183 175 163 117 99 99 85 39 37 BspEI T'CCGG_A Cuts at: 3226 4053 6492 3226 Size: 827 2439 3563 Fragments arranged by size: 3563 2439 827 BspHI T'CATG_A Cuts at: 4718 6448 4718 Size: 1730 5099 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 3000 3693 4110 4184 844 Size: 1520 636 693 417 74 3489 Fragments arranged by size: 3489 1520 693 636 417 74 BsrBI CCG'CTC Cuts at: 1394 1823 4716 1394 Size: 429 2893 3507 Fragments arranged by size: 3507 2893 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5324 5498 65 Size: 1183 4076 174 1396 Fragments arranged by size: 4076 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3411 3652 3939 4082 5473 5660 1700 Size: 110 1601 241 287 143 1391 187 2869 Fragments arranged by size: 2869 1601 1391 287 241 187 143 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6829 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6829 BssSI C'ACGA_G Cuts at: 4889 5901 4889 Size: 1012 5817 BstAPI GCAn_nnn'nTGC Cuts at: 794 2734 794 Size: 1940 4889 BstBI TT'CG_AA Cuts at: 2007 5633 2007 Size: 3626 3203 BstEII G'GTnAC_C Cuts at: 1184 3394 3769 1184 Size: 2210 375 4244 Fragments arranged by size: 4244 2210 375 BstYI r'GATC_y Cuts at: 1972 4159 4526 4911 4928 6380 6466 6478 6824 Size: 2187 367 385 17 1452 86 12 346 Cuts at: 6824 1972 Size: 1977 Fragments arranged by size: 2187 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2810 2810 Size: 6829 BtgI C'CryG_G Cuts at: 513 1065 2022 2067 2247 2535 3327 513 Size: 552 957 45 180 288 792 4015 Fragments arranged by size: 4015 957 792 552 288 180 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6090 Fragments arranged by size: 6090 683 56 BtsI GCAGTG_nn' Cuts at: 2453 3312 4007 4345 5139 5159 2453 Size: 859 695 338 794 20 4123 Fragments arranged by size: 4123 859 794 695 338 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6829 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6794 DraI TTT'AAA Cuts at: 4133 4133 Size: 6829 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5836 809 Size: 507 4520 1802 Fragments arranged by size: 4520 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2141 2188 2250 Size: 54 1425 50 275 18 311 47 62 Cuts at: 2250 3107 3501 3705 3810 4276 5165 6794 8 Size: 857 394 204 105 466 889 1629 43 Fragments arranged by size: 1629 1425 889 857 466 394 311 275 204 105 62 54 50 47 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4276 1537 Size: 275 2464 4090 Fragments arranged by size: 4090 2464 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3605 4759 1353 Size: 2252 1154 3423 Fragments arranged by size: 3423 2252 1154 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3887 5416 5790 5936 387 Size: 1064 138 2298 1529 374 146 1280 Fragments arranged by size: 2298 1529 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2755 2755 Size: 6829 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2143 2534 2609 2654 2738 2804 2860 Size: 393 511 391 75 45 84 66 56 Cuts at: 2860 2918 3068 3077 3194 3201 3335 3455 3650 Size: 58 150 9 117 7 134 120 195 Cuts at: 3650 3926 3986 4892 5489 6276 6544 1239 Size: 276 60 906 597 787 268 1524 Fragments arranged by size: 1524 906 787 597 511 393 391 276 268 195 150 134 120 117 84 75 66 60 58 56 45 9 7 EcoNI CCTnn'n_nnAGG Cuts at: 2189 2462 2591 2189 Size: 273 129 6427 Fragments arranged by size: 6427 273 129 EcoO109I rG'GnC_Cy Cuts at: 2058 2292 2629 3028 3169 3430 3486 3670 3874 Size: 234 337 399 141 261 56 184 204 Cuts at: 3874 2058 Size: 5013 Fragments arranged by size: 5013 399 337 261 234 204 184 141 56 EcoRI G'AATT_C Cuts at: 4138 4516 4138 Size: 378 6451 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2614 2935 2614 Size: 321 6508 FspI TGC'GCA Cuts at: 2803 5335 2803 Size: 2532 4297 HaeII r_GCGC'y Cuts at: 1678 2156 2715 3647 5976 1678 Size: 478 559 932 2329 2531 Fragments arranged by size: 2531 2329 932 559 478 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 2294 2326 2941 Size: 32 135 32 492 32 198 32 615 Cuts at: 2941 2973 5470 5502 5544 5576 1373 Size: 32 2497 32 42 32 2626 Fragments arranged by size: 2626 2497 615 492 198 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3343 4173 4419 669 Size: 988 281 1405 830 246 3079 Fragments arranged by size: 3079 1405 988 830 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6829 HpaI GTT'AAC Cuts at: 4419 4419 Size: 6829 KasI G'GCGC_C Cuts at: 2152 2152 Size: 6829 KpnI G_GTAC'C Cuts at: 3946 4154 3946 Size: 208 6621 MfeI C'AATT_G Cuts at: 4428 4428 Size: 6829 MluI A'CGCG_T Cuts at: 6705 6705 Size: 6829 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2265 5943 6127 1305 Size: 960 3678 184 2007 Fragments arranged by size: 3678 2007 960 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3707 10 Size: 54 1425 343 1875 3132 Fragments arranged by size: 3132 1875 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3305 3599 3752 4787 5146 5305 518 Size: 648 2139 294 153 1035 359 159 2042 Fragments arranged by size: 2139 2042 1035 648 359 294 159 153 MspA1I CmG'CkG Cuts at: 1606 2179 2734 3106 3184 3407 3904 4245 4922 Size: 573 555 372 78 223 497 341 677 Cuts at: 4922 6070 6315 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 573 555 497 372 341 245 223 78 NarI GG'CG_CC Cuts at: 2153 2153 Size: 6829 NcoI C'CATG_G Cuts at: 513 1065 2022 2067 2535 513 Size: 552 957 45 468 4807 Fragments arranged by size: 4807 957 552 468 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 6829 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6829 NotI GC'GGCC_GC Cuts at: 4276 4276 Size: 6829 NspI r_CATG'y Cuts at: 1132 1229 3164 4187 5732 1132 Size: 97 1935 1023 1545 2229 Fragments arranged by size: 2229 1935 1545 1023 97 PasI CC'CwG_GG Cuts at: 1348 3063 4066 1348 Size: 1715 1003 4111 Fragments arranged by size: 4111 1715 1003 PciI A'CATG_T Cuts at: 5728 5728 Size: 6829 PflMI CCAn_nnn'nTGG Cuts at: 1108 2406 1108 Size: 1298 5531 PfoI T'CCnGG_A Cuts at: 2315 3518 3552 2315 Size: 1203 34 5592 Fragments arranged by size: 5592 1203 34 PmeI GTTT'AAAC Cuts at: 4133 4133 Size: 6829 PmlI CAC'GTG Cuts at: 2857 2857 Size: 6829 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4898 4930 6411 6443 4898 Size: 32 1481 32 5284 Fragments arranged by size: 5284 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2292 2629 3028 3486 3874 2292 Size: 337 399 458 388 5247 Fragments arranged by size: 5247 458 399 388 337 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6829 PsiI TTA'TAA Cuts at: 4399 4399 Size: 6829 PspOMI G'GGCC_C Cuts at: 2058 2058 Size: 6829 PstI C_TGCA'G Cuts at: 830 2739 3532 3690 4181 830 Size: 1909 793 158 491 3478 Fragments arranged by size: 3478 1909 793 491 158 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6829 PvuII CAG'CTG Cuts at: 2179 2734 3184 3407 3904 2179 Size: 555 450 223 497 5104 Fragments arranged by size: 5104 555 497 450 223 SacI G_AGCT'C Cuts at: 2757 2757 Size: 6829 SalI G'TCGA_C Cuts at: 1936 4171 1936 Size: 2235 4594 SanDI GG'GwC_CC Cuts at: 2292 3028 3874 2292 Size: 736 846 5247 Fragments arranged by size: 5247 846 736 SapI GCTCTTCn'nnn_ Cuts at: 3605 3605 Size: 6829 SbfI CC_TGCA'GG Cuts at: 4181 4181 Size: 6829 ScaI AGT'ACT Cuts at: 5077 6789 5077 Size: 1712 5117 SexAI A'CCwGG_T Cuts at: 3564 3564 Size: 6829 SfcI C'TryA_G Cuts at: 826 1045 2101 2705 2735 2966 3372 3528 3686 Size: 219 1056 604 30 231 406 156 158 Cuts at: 3686 3737 3790 4177 5312 5993 6184 826 Size: 51 53 387 1135 681 191 1471 Fragments arranged by size: 1471 1135 1056 681 604 406 387 231 219 191 158 156 53 51 30 SfoI GGC'GCC Cuts at: 2154 2154 Size: 6829 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6829 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6829 SmaI CCC'GGG Cuts at: 1872 3358 4156 1872 Size: 1486 798 4545 Fragments arranged by size: 4545 1486 798 SmlI C'TyrA_G Cuts at: 820 1017 1943 4933 5834 6096 820 Size: 197 926 2990 901 262 1553 Fragments arranged by size: 2990 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4126 4620 493 Size: 3633 494 2702 Fragments arranged by size: 3633 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6829 SphI G_CATG'C Cuts at: 1132 4187 1132 Size: 3055 3774 SspI AAT'ATT Cuts at: 5 52 4753 5 Size: 47 4701 2081 Fragments arranged by size: 4701 2081 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 5749 StyI C'CwwG_G Cuts at: 513 1065 2022 2067 2535 3022 3110 3129 3639 Size: 552 957 45 468 487 88 19 510 Cuts at: 3639 3946 4111 4268 513 Size: 307 165 157 3074 Fragments arranged by size: 3074 957 552 510 487 468 307 165 157 88 45 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4856 4873 5026 5211 5656 325 Size: 1255 3276 17 153 185 445 1498 Fragments arranged by size: 3276 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5075 6787 96 Size: 274 80 33 51 1443 3098 1712 138 Fragments arranged by size: 3098 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2228 2260 6789 6821 2228 Size: 32 4529 32 2236 Fragments arranged by size: 4529 2236 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6829 XbaI T'CTAG_A Cuts at: 4165 4165 Size: 6829 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3160 3160 Size: 6829 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6829 XmaI C'CCGG_G Cuts at: 1870 3356 4154 1870 Size: 1486 798 4545 Fragments arranged by size: 4545 1486 798 XmnI GAAnn'nnTTC Cuts at: 2597 4958 2597 Size: 2361 4468 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4637 276 Size: 53 83 186 936 3103 2468 Fragments arranged by size: 3103 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SrfI SwaI