(Circular) (Six-base) MAPSORT of: pFN21AE2502.seq Check: 6403 from: 1 to: 6877 pFN21AE2502 6877 bp With 182 enzymes: SgfI * February 16, 2012 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2534 3254 2534 Size: 720 6157 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4687 278 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2634 2844 4220 1937 Size: 697 210 1376 4594 Fragments arranged by size: 4594 1376 697 210 Acc65I G'GTAC_C Cuts at: 4198 4198 Size: 6877 AclI AA'CG_TT Cuts at: 1563 5004 5377 1563 Size: 3441 373 3063 Fragments arranged by size: 3441 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2474 2486 2684 3092 3284 4940 6324 6592 Size: 842 12 198 408 192 1656 1384 268 Cuts at: 6592 1632 Size: 1917 Fragments arranged by size: 1917 1656 1384 842 408 268 198 192 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6680 AflIII A'CryG_T Cuts at: 1203 5776 6753 1203 Size: 4573 977 1327 Fragments arranged by size: 4573 1327 977 AgeI A'CCGG_T Cuts at: 5708 5708 Size: 6877 AhdI GACnn_n'nnGTC Cuts at: 5606 5606 Size: 6877 AleI CACnn'nnGTG Cuts at: 1166 3648 3938 1166 Size: 2482 290 4105 Fragments arranged by size: 4105 2482 290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3518 3812 1184 Size: 2334 294 4249 Fragments arranged by size: 4249 2334 294 AlwNI CAG_nnn'CTG Cuts at: 3322 4620 6192 3322 Size: 1298 1572 4007 Fragments arranged by size: 4007 1572 1298 ApaI G_GGCC'C Cuts at: 3043 3043 Size: 6877 ApaLI G'TGCA_C Cuts at: 3847 4934 6090 3847 Size: 1087 1156 4634 Fragments arranged by size: 4634 1156 1087 ApoI r'AATT_y Cuts at: 1493 2756 4186 4412 4564 6673 1493 Size: 1263 1430 226 152 2109 1697 Fragments arranged by size: 2109 1697 1430 1263 226 152 AseI AT'TA_AT Cuts at: 160 1032 3710 5431 160 Size: 872 2678 1721 1606 Fragments arranged by size: 2678 1721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2256 3132 4058 4202 1870 Size: 73 243 70 876 926 144 4545 Fragments arranged by size: 4545 926 876 243 144 73 70 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4126 4159 4126 Size: 33 6844 BamHI G'GATC_C Cuts at: 3996 4207 4574 3996 Size: 211 367 6299 Fragments arranged by size: 6299 367 211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2855 3127 3502 3696 4198 Size: 325 217 627 1068 272 375 194 502 Cuts at: 4198 5653 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1068 627 502 375 325 272 217 194 BanII G_rGCy'C Cuts at: 1391 2193 3043 3111 4196 1391 Size: 802 850 68 1085 4072 Fragments arranged by size: 4072 1085 850 802 68 BbeI G_GCGC'C Cuts at: 3700 3700 Size: 6877 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5906 BbvCI CC'TCA_GC Cuts at: 2987 2987 Size: 6877 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5068 5102 1903 Size: 34 50 34 3047 34 3678 Fragments arranged by size: 3678 3047 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2021 4769 5979 2021 Size: 2748 1210 2919 Fragments arranged by size: 2919 2748 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6877 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3567 5488 136 Size: 107 122 71 631 605 1895 1921 1525 Fragments arranged by size: 1921 1895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2667 3006 3555 6872 2667 Size: 339 549 3317 2672 Fragments arranged by size: 3317 2672 549 339 BlpI GC'TnA_GC Cuts at: 4294 4294 Size: 6877 Bme1580I G_kGCm'C Cuts at: 1790 2534 3043 3132 3507 3741 3750 3851 4938 Size: 744 509 89 375 234 9 101 1087 Cuts at: 4938 6094 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1087 744 509 375 234 101 89 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6877 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 2871 3332 Size: 416 53 83 399 24 798 656 461 Cuts at: 3332 3702 4608 5566 442 Size: 370 906 958 1753 Fragments arranged by size: 1753 958 906 798 656 461 416 399 370 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3305 1056 Size: 2249 4628 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2040 2217 5537 1239 Size: 801 177 3320 2579 Fragments arranged by size: 3320 2579 801 177 Bpu10I CC'TnA_GC Cuts at: 2987 3490 2987 Size: 503 6374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5002 5867 6165 5002 Size: 865 298 5714 Fragments arranged by size: 5714 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 3404 3431 5540 882 Size: 617 567 1338 27 2109 2219 Fragments arranged by size: 2219 2109 1338 617 567 27 BsaAI yAC'GTr Cuts at: 493 1204 2904 4668 493 Size: 711 1700 1764 2702 Fragments arranged by size: 2702 1764 1700 711 BsaBI GATnn'nnATC Cuts at: 6871 6871 Size: 6877 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2799 3066 3669 3697 Size: 53 83 186 936 1266 267 603 28 Cuts at: 3697 4684 5066 5701 275 Size: 987 382 635 1451 Fragments arranged by size: 1451 1266 987 936 635 603 382 267 186 83 53 28 BsaWI w'CCGG_w Cuts at: 3999 5310 5708 5982 6129 6540 3999 Size: 1311 398 274 147 411 4336 Fragments arranged by size: 4336 1311 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2231 2261 1178 Size: 30 165 30 828 30 5794 Fragments arranged by size: 5794 828 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2709 3770 1186 Size: 1066 457 1061 4293 Fragments arranged by size: 4293 1066 1061 457 BseYI C'CCAG_C Cuts at: 2051 2291 2767 2814 6080 6576 2051 Size: 240 476 47 3266 496 2352 Fragments arranged by size: 3266 2352 496 476 240 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3626 1163 Size: 573 1890 4414 Fragments arranged by size: 4414 1890 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2945 4327 5088 6116 1540 Size: 275 186 944 1382 761 1028 2301 Fragments arranged by size: 2301 1382 1028 944 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 3851 4938 5023 6094 2193 Size: 1658 1087 85 1071 2976 Fragments arranged by size: 2976 1658 1087 1071 85 BsmI GAATG_Cn' Cuts at: 4388 4481 4388 Size: 93 6784 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2534 3043 3111 3132 3507 3741 Size: 399 403 341 509 68 21 375 234 Cuts at: 3741 3750 3851 4196 4938 5023 6094 1391 Size: 9 101 345 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 509 403 399 375 345 341 234 101 85 68 21 9 BspEI T'CCGG_A Cuts at: 3999 6540 3999 Size: 2541 4336 BspHI T'CATG_A Cuts at: 4766 6496 4766 Size: 1730 5147 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2430 2534 2988 3254 4232 844 Size: 1586 104 454 266 978 3489 Fragments arranged by size: 3489 1586 978 454 266 104 BsrBI CCG'CTC Cuts at: 1394 1823 3359 4764 1394 Size: 429 1536 1405 3507 Fragments arranged by size: 3507 1536 1405 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5372 5546 65 Size: 1183 4124 174 1396 Fragments arranged by size: 4124 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2088 2930 2944 3477 5521 5708 1700 Size: 110 278 842 14 533 2044 187 2869 Fragments arranged by size: 2869 2044 842 533 278 187 110 14 BsrGI T'GTAC_A Cuts at: 96 2742 3483 96 Size: 2646 741 3490 Fragments arranged by size: 3490 2646 741 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6877 BssSI C'ACGA_G Cuts at: 4937 5949 4937 Size: 1012 5865 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6877 BstBI TT'CG_AA Cuts at: 5681 5681 Size: 6877 BstEII G'GTnAC_C Cuts at: 1184 3223 1184 Size: 2039 4838 BstXI CCAn_nnnn'nTGG Cuts at: 2059 3583 2059 Size: 1524 5353 BstYI r'GATC_y Cuts at: 1972 2658 2667 3006 3555 3996 4207 4574 4959 Size: 686 9 339 549 441 211 367 385 Cuts at: 4959 4976 6428 6514 6526 6872 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 686 549 441 385 367 346 339 211 86 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2784 3442 2784 Size: 658 6219 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2347 2584 487 Size: 683 56 1121 237 4780 Fragments arranged by size: 4780 1121 683 237 56 BtsI GCAGTG_nn' Cuts at: 2532 4393 5187 5207 2532 Size: 1861 794 20 4202 Fragments arranged by size: 4202 1861 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6877 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6842 DraI TTT'AAA Cuts at: 4181 4181 Size: 6877 DraIII CAC_nnn'GTG Cuts at: 2482 2482 Size: 6877 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5884 809 Size: 507 4568 1802 Fragments arranged by size: 4568 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2484 2733 2942 Size: 54 1425 50 275 18 654 249 209 Cuts at: 2942 3658 4324 5213 6842 8 Size: 716 666 889 1629 43 Fragments arranged by size: 1629 1425 889 716 666 654 275 249 209 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2942 4324 1537 Size: 275 1130 1382 4090 Fragments arranged by size: 4090 1382 1130 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3984 4807 1353 Size: 2631 823 3423 Fragments arranged by size: 3423 2631 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5464 5838 5984 387 Size: 1064 138 3875 374 146 1280 Fragments arranged by size: 3875 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2191 2191 Size: 6877 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2040 2217 2474 2486 2684 3092 3284 Size: 393 408 177 257 12 198 408 192 Cuts at: 3284 4940 5537 6324 6592 1239 Size: 1656 597 787 268 1524 Fragments arranged by size: 1656 1524 787 597 408 408 393 268 257 198 192 177 12 EcoNI CCTnn'n_nnAGG Cuts at: 2468 2468 Size: 6877 EcoO109I rG'GnC_Cy Cuts at: 2047 2338 2356 3039 3446 4094 2047 Size: 291 18 683 407 648 4830 Fragments arranged by size: 4830 683 648 407 291 18 EcoRI G'AATT_C Cuts at: 2756 4186 4564 2756 Size: 1430 378 5069 Fragments arranged by size: 5069 1430 378 FspI TGC'GCA Cuts at: 2282 5383 2282 Size: 3101 3776 HaeII r_GCGC'y Cuts at: 1678 2246 2383 3700 6024 1678 Size: 568 137 1317 2324 2531 Fragments arranged by size: 2531 2324 1317 568 137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2872 2904 3098 3130 3868 Size: 32 135 32 1300 32 194 32 738 Cuts at: 3868 3900 5518 5550 5592 5624 1373 Size: 32 1618 32 42 32 2626 Fragments arranged by size: 2626 1618 1300 738 194 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2635 4009 4221 4467 669 Size: 988 281 697 1374 212 246 3079 Fragments arranged by size: 3079 1374 988 697 281 246 212 HindIII A'AGCT_T Cuts at: 748 748 Size: 6877 HpaI GTT'AAC Cuts at: 4467 4467 Size: 6877 KasI G'GCGC_C Cuts at: 3696 3696 Size: 6877 KpnI G_GTAC'C Cuts at: 4202 4202 Size: 6877 MfeI C'AATT_G Cuts at: 4476 4476 Size: 6877 MluI A'CGCG_T Cuts at: 6753 6753 Size: 6877 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5991 6175 1305 Size: 4686 184 2007 Fragments arranged by size: 4686 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2486 2735 10 Size: 54 1425 343 654 249 4152 Fragments arranged by size: 4152 1425 654 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2102 3125 3221 3581 3648 3938 4835 Size: 648 936 1023 96 360 67 290 897 Cuts at: 4835 5194 5353 518 Size: 359 159 2042 Fragments arranged by size: 2042 1023 936 897 648 360 359 290 159 96 67 MspA1I CmG'CkG Cuts at: 1606 2295 2650 3722 4293 4970 6118 6363 1606 Size: 689 355 1072 571 677 1148 245 2120 Fragments arranged by size: 2120 1148 1072 689 677 571 355 245 NaeI GCC'GGC Cuts at: 2090 2946 2090 Size: 856 6021 NarI GG'CG_CC Cuts at: 3697 3697 Size: 6877 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6325 NdeI CA'TA_TG Cuts at: 387 387 Size: 6877 NgoMIV G'CCGG_C Cuts at: 2088 2944 2088 Size: 856 6021 NheI G'CTAG_C Cuts at: 1052 3301 1052 Size: 2249 4628 NotI GC'GGCC_GC Cuts at: 4324 4324 Size: 6877 NspI r_CATG'y Cuts at: 1132 1229 2059 4235 5780 1132 Size: 97 830 2176 1545 2229 Fragments arranged by size: 2229 2176 1545 830 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6877 PciI A'CATG_T Cuts at: 5776 5776 Size: 6877 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 3169 3322 3919 1108 Size: 1374 687 153 597 4066 Fragments arranged by size: 4066 1374 687 597 153 PfoI T'CCnGG_A Cuts at: 3162 3162 Size: 6877 PmeI GTTT'AAAC Cuts at: 4181 4181 Size: 6877 PmlI CAC'GTG Cuts at: 2904 2904 Size: 6877 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4946 4978 6459 6491 4946 Size: 32 1481 32 5332 Fragments arranged by size: 5332 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2356 4094 2047 Size: 309 1738 4830 Fragments arranged by size: 4830 1738 309 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6877 PsiI TTA'TAA Cuts at: 4447 4447 Size: 6877 PspOMI G'GGCC_C Cuts at: 3039 3039 Size: 6877 PstI C_TGCA'G Cuts at: 830 4229 830 Size: 3399 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6877 PvuII CAG'CTG Cuts at: 2295 2650 3722 2295 Size: 355 1072 5450 Fragments arranged by size: 5450 1072 355 SacI G_AGCT'C Cuts at: 2193 2193 Size: 6877 SalI G'TCGA_C Cuts at: 1936 2633 4219 1936 Size: 697 1586 4594 Fragments arranged by size: 4594 1586 697 SanDI GG'GwC_CC Cuts at: 2047 4094 2047 Size: 2047 4830 SbfI CC_TGCA'GG Cuts at: 4229 4229 Size: 6877 ScaI AGT'ACT Cuts at: 5125 6837 5125 Size: 1712 5165 SexAI A'CCwGG_T Cuts at: 3294 3294 Size: 6877 SfcI C'TryA_G Cuts at: 826 1045 4225 5360 6041 6232 826 Size: 219 3180 1135 681 191 1471 Fragments arranged by size: 3180 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3698 3698 Size: 6877 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6877 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6877 SmaI CCC'GGG Cuts at: 1872 3134 4204 1872 Size: 1262 1070 4545 Fragments arranged by size: 4545 1262 1070 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 4058 4981 5882 6144 820 Size: 197 926 243 1872 923 901 262 1553 Fragments arranged by size: 1872 1553 926 923 901 262 243 197 SnaBI TAC'GTA Cuts at: 493 4668 493 Size: 4175 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6877 SphI G_CATG'C Cuts at: 1132 2059 4235 1132 Size: 927 2176 3774 Fragments arranged by size: 3774 2176 927 SspI AAT'ATT Cuts at: 5 52 4801 5 Size: 47 4749 2081 Fragments arranged by size: 4749 2081 47 StuI AGG'CCT Cuts at: 1517 2588 1517 Size: 1071 5806 StyI C'CwwG_G Cuts at: 513 1065 2442 2871 3228 3306 4316 513 Size: 552 1377 429 357 78 1010 3074 Fragments arranged by size: 3074 1377 1010 552 429 357 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2129 2828 4904 4921 5074 5259 5704 Size: 1255 549 699 2076 17 153 185 445 Cuts at: 5704 325 Size: 1498 Fragments arranged by size: 2076 1498 1255 699 549 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2595 2742 3483 Size: 274 80 33 51 1443 618 147 741 Cuts at: 3483 4134 5123 6835 96 Size: 651 989 1712 138 Fragments arranged by size: 1712 1443 989 741 651 618 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2231 2263 3443 3475 6837 6869 2231 Size: 32 1180 32 3362 32 2239 Fragments arranged by size: 3362 2239 1180 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6877 XbaI T'CTAG_A Cuts at: 4213 4213 Size: 6877 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2626 3118 2626 Size: 492 6385 XhoI C'TCGA_G Cuts at: 1943 2186 4058 1943 Size: 243 1872 4762 Fragments arranged by size: 4762 1872 243 XmaI C'CCGG_G Cuts at: 1870 3132 4202 1870 Size: 1262 1070 4545 Fragments arranged by size: 4545 1262 1070 XmnI GAAnn'nnTTC Cuts at: 3269 5006 3269 Size: 1737 5140 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4685 276 Size: 53 83 186 936 3151 2468 Fragments arranged by size: 3151 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfiI SrfI SwaI