(Circular) (Six-base) MAPSORT of: pFN21ASDA0626.seq Check: 596 from: 1 to: 5956 pFN21ASDA0626 5956 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3233 3233 Size: 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2475 2880 3249 3299 1937 Size: 538 405 369 50 4594 Fragments arranged by size: 4594 538 405 369 50 Acc65I G'GTAC_C Cuts at: 3154 3277 3154 Size: 123 5833 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2921 3153 4019 5403 5671 1632 Size: 1289 232 866 1384 268 1917 Fragments arranged by size: 1917 1384 1289 866 268 232 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 2391 2393 4855 5832 1203 Size: 1188 2 2462 977 1327 Fragments arranged by size: 2462 1327 1188 977 2 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 2141 2396 1166 Size: 975 255 4726 Fragments arranged by size: 4726 975 255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5956 AlwNI CAG_nnn'CTG Cuts at: 3699 5271 3699 Size: 1572 4384 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 2827 3265 3491 3643 5752 1493 Size: 1334 438 226 152 2109 1697 Fragments arranged by size: 2109 1697 1334 438 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2349 2382 2349 Size: 33 5923 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 3154 3277 4732 618 Size: 325 217 627 630 737 123 1455 1842 Fragments arranged by size: 1842 1455 737 630 627 325 217 123 BanII G_rGCy'C Cuts at: 1391 2156 3275 1391 Size: 765 1119 4072 Fragments arranged by size: 4072 1119 765 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2570 928 Size: 971 671 4314 Fragments arranged by size: 4314 971 671 BbvCI CC'TCA_GC Cuts at: 2958 2958 Size: 5956 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2714 2748 2900 2934 4147 4181 1903 Size: 34 777 34 152 34 1213 34 3678 Fragments arranged by size: 3678 1213 777 152 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2098 2377 3848 5058 2098 Size: 279 1471 1210 2996 Fragments arranged by size: 2996 1471 1210 279 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4567 136 Size: 107 122 71 631 605 2895 1525 Fragments arranged by size: 2895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2073 2163 4017 5173 1790 Size: 283 90 1854 1156 2573 Fragments arranged by size: 2573 1854 1156 283 90 BmgBI CAC'GTC Cuts at: 1318 3114 1318 Size: 1796 4160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2686 3687 4645 Size: 416 53 83 399 24 1269 1001 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1269 1001 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2763 2859 2763 Size: 96 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2481 2829 2831 4616 1239 Size: 1242 348 2 1785 2579 Fragments arranged by size: 2579 1785 1242 348 2 Bpu10I CC'TnA_GC Cuts at: 2958 2958 Size: 5956 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4619 882 Size: 617 3120 2219 Fragments arranged by size: 3120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2394 3253 3747 493 Size: 711 1190 859 494 2702 Fragments arranged by size: 2702 1190 859 711 494 BsaBI GATnn'nnATC Cuts at: 5950 5950 Size: 5956 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2934 3763 4145 4780 Size: 53 83 186 936 1401 829 382 635 Cuts at: 4780 275 Size: 1451 Fragments arranged by size: 1451 1401 936 829 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5731 Fragments arranged by size: 5731 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2328 2331 2801 2828 3068 1186 Size: 1142 3 470 27 240 4074 Fragments arranged by size: 4074 1142 470 240 27 3 BseYI C'CCAG_C Cuts at: 5159 5655 5159 Size: 496 5460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2625 2930 1163 Size: 573 889 305 4189 Fragments arranged by size: 4189 889 573 305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4017 4102 5173 4017 Size: 85 1071 4800 Fragments arranged by size: 4800 1071 85 BsmI GAATG_Cn' Cuts at: 3467 3560 3467 Size: 93 5863 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2156 2163 3275 4017 4102 5173 Size: 399 283 83 7 1112 742 85 1071 Cuts at: 5173 1391 Size: 2174 Fragments arranged by size: 2174 1112 1071 742 399 283 85 83 7 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 3845 5575 3845 Size: 1730 4226 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3233 3311 844 Size: 2389 78 3489 Fragments arranged by size: 3489 2389 78 BsrBI CCG'CTC Cuts at: 1394 1823 3843 1394 Size: 429 2020 3507 Fragments arranged by size: 3507 2020 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2169 4451 4625 65 Size: 1183 921 2282 174 1396 Fragments arranged by size: 2282 1396 1183 921 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4600 4787 1700 Size: 110 2790 187 2869 Fragments arranged by size: 2869 2790 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2948 4016 5028 2948 Size: 1068 1012 3876 Fragments arranged by size: 3876 1068 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 2007 4760 2007 Size: 2753 3203 BstEII G'GTnAC_C Cuts at: 1184 2434 1184 Size: 1250 4706 BstXI CCAn_nnnn'nTGG Cuts at: 2515 3026 2515 Size: 511 5445 BstYI r'GATC_y Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951 Size: 1314 367 385 17 1452 86 12 346 Cuts at: 5951 1972 Size: 1977 Fragments arranged by size: 1977 1452 1314 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2703 513 Size: 552 957 681 3766 Fragments arranged by size: 3766 957 681 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2925 487 Size: 683 56 1699 3518 Fragments arranged by size: 3518 1699 683 56 BtsI GCAGTG_nn' Cuts at: 3210 3472 4266 4286 3210 Size: 262 794 20 4880 Fragments arranged by size: 4880 794 262 20 ClaI AT'CG_AT Cuts at: 1554 2029 1554 Size: 475 5481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 2130 3260 2130 Size: 1130 4826 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 3064 4963 809 Size: 507 1155 593 1899 1802 Fragments arranged by size: 1899 1802 1155 593 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3403 4292 5921 Size: 54 1425 50 275 18 1573 889 1629 Cuts at: 5921 8 Size: 43 Fragments arranged by size: 1629 1573 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2030 2289 2721 3129 3886 1353 Size: 677 259 432 408 757 3423 Fragments arranged by size: 3423 757 677 432 408 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2650 3086 4543 4917 5063 387 Size: 1064 138 1061 436 1457 374 146 1280 Fragments arranged by size: 1457 1280 1064 1061 436 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2481 2829 2831 2921 3153 4019 4616 Size: 393 849 348 2 90 232 866 597 Cuts at: 4616 5403 5671 1239 Size: 787 268 1524 Fragments arranged by size: 1524 866 849 787 597 393 348 268 232 90 2 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2386 2386 Size: 5956 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 5103 1678 Size: 3425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2539 2571 2924 2956 3098 Size: 32 135 32 967 32 353 32 142 Cuts at: 3098 3113 3130 3145 4597 4629 4671 4703 1373 Size: 15 17 15 1452 32 42 32 2626 Fragments arranged by size: 2626 1452 967 353 142 135 42 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KpnI G_GTAC'C Cuts at: 3158 3281 3158 Size: 123 5833 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5070 5254 1305 Size: 3765 184 2007 Fragments arranged by size: 3765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4134 Fragments arranged by size: 4134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2141 2396 2486 2513 2975 3012 3914 Size: 648 975 255 90 27 462 37 902 Cuts at: 3914 4273 4432 518 Size: 359 159 2042 Fragments arranged by size: 2042 975 902 648 462 359 255 159 90 37 27 MspA1I CmG'CkG Cuts at: 1606 2343 2924 3372 4049 5197 5442 1606 Size: 737 581 448 677 1148 245 2120 Fragments arranged by size: 2120 1148 737 677 581 448 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2703 513 Size: 552 957 681 3766 Fragments arranged by size: 3766 957 681 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3011 3314 4859 1132 Size: 97 1782 303 1545 2229 Fragments arranged by size: 2229 1782 1545 303 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PmlI CAC'GTG Cuts at: 2394 2394 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5956 PsiI TTA'TAA Cuts at: 3526 3526 Size: 5956 PstI C_TGCA'G Cuts at: 830 2619 2774 3094 3308 830 Size: 1789 155 320 214 3478 Fragments arranged by size: 3478 1789 320 214 155 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SapI GCTCTTCn'nnn_ Cuts at: 2030 2721 3129 2030 Size: 691 408 4857 Fragments arranged by size: 4857 691 408 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SfcI C'TryA_G Cuts at: 826 1045 2224 2615 2770 3090 3304 4439 5120 Size: 219 1179 391 155 320 214 1135 681 Cuts at: 5120 5311 826 Size: 191 1471 Fragments arranged by size: 1471 1179 1135 681 391 320 219 214 191 155 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3253 3747 493 Size: 2760 494 2702 Fragments arranged by size: 2760 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3011 3314 1132 Size: 1879 303 3774 Fragments arranged by size: 3774 1879 303 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5956 StyI C'CwwG_G Cuts at: 513 1065 2022 2361 2703 3395 513 Size: 552 957 339 342 692 3074 Fragments arranged by size: 3074 957 692 552 342 339 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2394 2459 2612 2705 3983 4000 4153 Size: 1255 814 65 153 93 1278 17 153 Cuts at: 4153 4338 4783 325 Size: 185 445 1498 Fragments arranged by size: 1498 1278 1255 814 445 185 153 153 93 65 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4202 5914 96 Size: 274 80 33 51 1443 2225 1712 138 Fragments arranged by size: 2225 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2587 2619 2978 3010 5916 5948 2587 Size: 32 359 32 2906 32 2595 Fragments arranged by size: 2906 2595 359 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 2671 3292 2671 Size: 621 5335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2256 2256 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI