(Circular) (Six-base) MAPSORT of: pFN21ASDA0615.seq Check: 4290 from: 1 to: 7417 pFN21ASDA0615 7417 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5227 278 Size: 53 83 186 936 3691 2468 Fragments arranged by size: 3691 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4760 1937 Size: 2823 4594 Acc65I G'GTAC_C Cuts at: 4738 4738 Size: 7417 AclI AA'CG_TT Cuts at: 1563 3655 5544 5917 1563 Size: 2092 1889 373 3063 Fragments arranged by size: 3063 2092 1889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 4319 4396 4628 5480 6864 7132 1632 Size: 1281 1406 77 232 852 1384 268 1917 Fragments arranged by size: 1917 1406 1384 1281 852 268 232 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7220 AflIII A'CryG_T Cuts at: 1203 4260 6316 7293 1203 Size: 3057 2056 977 1327 Fragments arranged by size: 3057 2056 1327 977 AgeI A'CCGG_T Cuts at: 6248 6248 Size: 7417 AhdI GACnn_n'nnGTC Cuts at: 6146 6146 Size: 7417 AleI CACnn'nnGTG Cuts at: 1166 4265 1166 Size: 3099 4318 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2195 1184 Size: 1011 6406 AlwNI CAG_nnn'CTG Cuts at: 3482 3794 4549 5160 6732 3482 Size: 312 755 611 1572 4167 Fragments arranged by size: 4167 1572 755 611 312 ApaLI G'TGCA_C Cuts at: 5474 6630 5474 Size: 1156 6261 ApoI r'AATT_y Cuts at: 1493 2498 2862 3019 3209 3344 4202 4726 4952 Size: 1005 364 157 190 135 858 524 226 Cuts at: 4952 5104 7213 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1005 858 524 364 226 190 157 152 135 AseI AT'TA_AT Cuts at: 160 1032 3402 5971 160 Size: 872 2370 2569 1606 Fragments arranged by size: 2569 2370 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7417 AvaI C'yCGr_G Cuts at: 1870 1943 2031 4742 1870 Size: 73 88 2711 4545 Fragments arranged by size: 4545 2711 88 73 BamHI G'GATC_C Cuts at: 4747 5114 4747 Size: 367 7050 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4424 4738 6193 618 Size: 325 217 627 2637 314 1455 1842 Fragments arranged by size: 2637 1842 1455 627 325 314 217 BanII G_rGCy'C Cuts at: 1391 4074 4477 4736 1391 Size: 2683 403 259 4072 Fragments arranged by size: 4072 2683 403 259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3207 928 Size: 971 1308 5138 Fragments arranged by size: 5138 1308 971 BbvCI CC'TCA_GC Cuts at: 2151 2394 4497 4592 2151 Size: 243 2103 95 4976 Fragments arranged by size: 4976 2103 243 95 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2036 2070 5608 5642 1903 Size: 34 99 34 3538 34 3678 Fragments arranged by size: 3678 3538 99 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2329 3240 5309 6519 2329 Size: 911 2069 1210 3227 Fragments arranged by size: 3227 2069 1210 911 BclI T'GATC_A Cuts at: 1548 3772 1548 Size: 2224 5193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2115 2376 6028 Size: 107 122 71 631 605 443 261 3652 Cuts at: 6028 136 Size: 1525 Fragments arranged by size: 3652 1525 631 605 443 261 122 107 71 BglII A'GATC_T Cuts at: 4568 4611 4650 4678 7412 4568 Size: 43 39 28 2734 4573 Fragments arranged by size: 4573 2734 43 39 28 BlpI GC'TnA_GC Cuts at: 4834 4834 Size: 7417 Bme1580I G_kGCm'C Cuts at: 1790 4381 5478 6634 1790 Size: 2591 1097 1156 2573 Fragments arranged by size: 2591 2573 1156 1097 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2460 3804 4527 Size: 416 53 83 399 24 1043 1344 723 Cuts at: 4527 5148 6106 442 Size: 621 958 1753 Fragments arranged by size: 1753 1344 1043 958 723 621 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7417 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2667 4237 2667 Size: 1570 5847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 4049 4523 6077 1239 Size: 863 1947 474 1554 2579 Fragments arranged by size: 2579 1947 1554 863 474 Bpu10I CC'TnA_GC Cuts at: 2151 2394 4497 4592 2151 Size: 243 2103 95 4976 Fragments arranged by size: 4976 2103 243 95 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5542 6407 6705 5542 Size: 865 298 6254 Fragments arranged by size: 6254 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3230 6080 882 Size: 617 1731 2850 2219 Fragments arranged by size: 2850 2219 1731 617 BsaAI yAC'GTr Cuts at: 493 1204 4714 5208 493 Size: 711 3510 494 2702 Fragments arranged by size: 3510 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7411 7411 Size: 7417 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5224 5606 6241 275 Size: 53 83 186 936 3691 382 635 1451 Fragments arranged by size: 3691 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5850 6248 6522 6669 7080 5850 Size: 398 274 147 411 6187 Fragments arranged by size: 6187 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3691 3721 1178 Size: 30 165 30 2288 30 4874 Fragments arranged by size: 4874 2288 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 2615 3666 4340 1186 Size: 982 447 1051 674 4263 Fragments arranged by size: 4263 1051 982 674 447 BseYI C'CCAG_C Cuts at: 2741 3726 4059 4406 6620 7116 2741 Size: 985 333 347 2214 496 3042 Fragments arranged by size: 3042 2214 985 496 347 333 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6844 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2098 4867 5628 6656 1540 Size: 275 186 97 2769 761 1028 2301 Fragments arranged by size: 2769 2301 1028 761 275 186 97 BsiHKAI G_wGCw'C Cuts at: 2136 2211 4074 5478 5563 6634 2136 Size: 75 1863 1404 85 1071 2919 Fragments arranged by size: 2919 1863 1404 1071 85 75 BsmI GAATG_Cn' Cuts at: 2355 2680 4928 5021 2355 Size: 325 2248 93 4751 Fragments arranged by size: 4751 2248 325 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2136 2211 4074 4381 4477 4736 5478 Size: 399 346 75 1863 307 96 259 742 Cuts at: 5478 5563 6634 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1863 1071 742 399 346 307 259 96 85 75 BspEI T'CCGG_A Cuts at: 7080 7080 Size: 7417 BspHI T'CATG_A Cuts at: 4129 5306 7036 4129 Size: 1177 1730 4510 Fragments arranged by size: 4510 1730 1177 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4557 4772 844 Size: 3713 215 3489 Fragments arranged by size: 3713 3489 215 BsrBI CCG'CTC Cuts at: 1394 1823 5304 1394 Size: 429 3481 3507 Fragments arranged by size: 3507 3481 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3904 5912 6086 65 Size: 1183 2656 2008 174 1396 Fragments arranged by size: 2656 2008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6061 6248 1700 Size: 110 4251 187 2869 Fragments arranged by size: 4251 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7417 BssHII G'CGCG_C Cuts at: 1925 2139 2168 1925 Size: 214 29 7174 Fragments arranged by size: 7174 214 29 BssSI C'ACGA_G Cuts at: 5477 6489 5477 Size: 1012 6405 BstAPI GCAn_nnn'nTGC Cuts at: 794 4549 794 Size: 3755 3662 BstBI TT'CG_AA Cuts at: 2007 6221 2007 Size: 4214 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7417 BstXI CCAn_nnnn'nTGG Cuts at: 4705 4705 Size: 7417 BstYI r'GATC_y Cuts at: 1972 4568 4611 4650 4678 4747 5114 5499 5516 Size: 2596 43 39 28 69 367 385 17 Cuts at: 5516 6968 7054 7066 7412 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2596 1977 1452 385 367 346 86 69 43 39 28 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2092 3090 3913 4698 513 Size: 552 957 70 998 823 785 3232 Fragments arranged by size: 3232 998 957 823 785 552 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6678 Fragments arranged by size: 6678 683 56 BtsI GCAGTG_nn' Cuts at: 2052 2284 3719 4514 4933 5727 5747 2052 Size: 232 1435 795 419 794 20 3722 Fragments arranged by size: 3722 1435 795 794 419 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7417 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4150 4185 529 Size: 35 3586 35 3761 Fragments arranged by size: 3761 3586 35 35 DraI TTT'AAA Cuts at: 3085 4721 3085 Size: 1636 5781 DraIII CAC_nnn'GTG Cuts at: 2597 2597 Size: 7417 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6424 809 Size: 507 5108 1802 Fragments arranged by size: 5108 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 4864 5753 Size: 54 1425 50 275 18 265 2769 889 Cuts at: 5753 7382 8 Size: 1629 43 Fragments arranged by size: 2769 1629 1425 889 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2095 4864 1537 Size: 275 283 2769 4090 Fragments arranged by size: 4090 2769 283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2718 2835 5347 1353 Size: 1365 117 2512 3423 Fragments arranged by size: 3423 2512 1365 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2219 6004 6378 6524 387 Size: 1064 138 498 132 3785 374 146 1280 Fragments arranged by size: 3785 1280 1064 498 374 146 138 132 EcoICRI GAG'CTC Cuts at: 4072 4072 Size: 7417 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2913 4049 4319 4396 4523 4628 Size: 393 470 811 1136 270 77 127 105 Cuts at: 4628 5480 6077 6864 7132 1239 Size: 852 597 787 268 1524 Fragments arranged by size: 1524 1136 852 811 787 597 470 393 270 268 127 105 77 EcoO109I rG'GnC_Cy Cuts at: 2107 3764 2107 Size: 1657 5760 EcoRI G'AATT_C Cuts at: 2498 4726 5104 2498 Size: 2228 378 4811 Fragments arranged by size: 4811 2228 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2864 3570 4140 4367 2864 Size: 706 570 227 5914 Fragments arranged by size: 5914 706 570 227 FspI TGC'GCA Cuts at: 5923 5923 Size: 7417 HaeII r_GCGC'y Cuts at: 1678 2217 6564 1678 Size: 539 4347 2531 Fragments arranged by size: 4347 2531 539 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2851 2883 3740 3772 6058 Size: 32 135 32 1279 32 857 32 2286 Cuts at: 6058 6090 6132 6164 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2286 1279 857 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4761 5007 669 Size: 988 281 2823 246 3079 Fragments arranged by size: 3079 2823 988 281 246 HindIII A'AGCT_T Cuts at: 748 2323 3974 748 Size: 1575 1651 4191 Fragments arranged by size: 4191 1651 1575 HpaI GTT'AAC Cuts at: 5007 5007 Size: 7417 KpnI G_GTAC'C Cuts at: 4742 4742 Size: 7417 MfeI C'AATT_G Cuts at: 3951 5016 3951 Size: 1065 6352 MluI A'CGCG_T Cuts at: 7293 7293 Size: 7417 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4481 4686 6531 6715 1305 Size: 3176 205 1845 184 2007 Fragments arranged by size: 3176 2007 1845 205 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5595 Fragments arranged by size: 5595 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3128 4178 4265 4280 5375 5734 5893 Size: 648 1962 1050 87 15 1095 359 159 Cuts at: 5893 518 Size: 2042 Fragments arranged by size: 2042 1962 1095 1050 648 359 159 87 15 MspA1I CmG'CkG Cuts at: 1606 2063 2094 2161 3218 3730 4833 5510 6658 Size: 457 31 67 1057 512 1103 677 1148 Cuts at: 6658 6903 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1103 1057 677 512 457 245 67 31 NcoI C'CATG_G Cuts at: 513 1065 2022 3090 3913 4698 513 Size: 552 957 1068 823 785 3232 Fragments arranged by size: 3232 1068 957 823 785 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7417 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7417 NotI GC'GGCC_GC Cuts at: 4864 4864 Size: 7417 NspI r_CATG'y Cuts at: 1132 1229 2284 4399 4775 6320 1132 Size: 97 1055 2115 376 1545 2229 Fragments arranged by size: 2229 2115 1545 1055 376 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7417 PciI A'CATG_T Cuts at: 6316 6316 Size: 7417 PflMI CCAn_nnn'nTGG Cuts at: 1108 2822 4654 1108 Size: 1714 1832 3871 Fragments arranged by size: 3871 1832 1714 PfoI T'CCnGG_A Cuts at: 4065 4065 Size: 7417 PmeI GTTT'AAAC Cuts at: 4721 4721 Size: 7417 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5486 5518 6999 7031 5486 Size: 32 1481 32 5872 Fragments arranged by size: 5872 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3764 3764 Size: 7417 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7417 PsiI TTA'TAA Cuts at: 4987 4987 Size: 7417 PstI C_TGCA'G Cuts at: 830 2184 4257 4579 4769 830 Size: 1354 2073 322 190 3478 Fragments arranged by size: 3478 2073 1354 322 190 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7417 PvuII CAG'CTG Cuts at: 2063 3218 3730 2063 Size: 1155 512 5750 Fragments arranged by size: 5750 1155 512 SacI G_AGCT'C Cuts at: 4074 4074 Size: 7417 SacII CC_GC'GG Cuts at: 2095 2095 Size: 7417 SalI G'TCGA_C Cuts at: 1936 4759 1936 Size: 2823 4594 SbfI CC_TGCA'GG Cuts at: 4769 4769 Size: 7417 ScaI AGT'ACT Cuts at: 5665 7377 5665 Size: 1712 5705 SfcI C'TryA_G Cuts at: 826 1045 2180 4253 4575 4765 5900 6581 6772 Size: 219 1135 2073 322 190 1135 681 191 Cuts at: 6772 826 Size: 1471 Fragments arranged by size: 2073 1471 1135 1135 681 322 219 191 190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7417 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7417 SmaI CCC'GGG Cuts at: 1872 2033 4744 1872 Size: 161 2711 4545 Fragments arranged by size: 4545 2711 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 5521 6422 6684 820 Size: 197 926 3578 901 262 1553 Fragments arranged by size: 3578 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4714 5208 493 Size: 4221 494 2702 Fragments arranged by size: 4221 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7417 SphI G_CATG'C Cuts at: 1132 4775 1132 Size: 3643 3774 SrfI GCCC'GGGC Cuts at: 2033 2033 Size: 7417 SspI AAT'ATT Cuts at: 5 52 2695 3965 4080 5341 5 Size: 47 2643 1270 115 1261 2081 Fragments arranged by size: 2643 2081 1270 1261 115 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7417 StyI C'CwwG_G Cuts at: 513 1065 2022 2217 2274 3090 3913 4698 4856 Size: 552 957 195 57 816 823 785 158 Cuts at: 4856 513 Size: 3074 Fragments arranged by size: 3074 957 823 816 785 552 195 158 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4442 4680 5444 5461 5614 5799 6244 Size: 1255 2862 238 764 17 153 185 445 Cuts at: 6244 325 Size: 1498 Fragments arranged by size: 2862 1498 1255 764 445 238 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4257 5663 7375 Size: 274 80 33 51 1443 2280 1406 1712 Cuts at: 7375 96 Size: 138 Fragments arranged by size: 2280 1712 1443 1406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7377 7409 7377 Size: 32 7385 Tth111I GACn'n_nGTC Cuts at: 1316 2780 1316 Size: 1464 5953 XbaI T'CTAG_A Cuts at: 2699 4753 2699 Size: 2054 5363 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7417 XmaI C'CCGG_G Cuts at: 1870 2031 4742 1870 Size: 161 2711 4545 Fragments arranged by size: 4545 2711 161 XmnI GAAnn'nnTTC Cuts at: 2931 3926 4123 4602 4613 5546 2931 Size: 995 197 479 11 933 4802 Fragments arranged by size: 4802 995 933 479 197 11 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5225 276 Size: 53 83 186 936 3691 2468 Fragments arranged by size: 3691 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SwaI XcmI