Restriction map for
pFN21ASDA0615
(Linear) (Six-base) MAPSORT of: KIAA0615.seq Check: 5763 from: 1 to: 2688
KIAA0615.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AclI AA'CG_TT
Cuts at: 0 1632 2688
Size: 1632 1056
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 2296 2373 2605 2688
Size: 890 1406 77 232 83
Fragments arranged by size:
1406 890 232 83 77
AflIII A'CryG_T
Cuts at: 0 2237 2688
Size: 2237 451
AleI CACnn'nnGTG
Cuts at: 0 2242 2688
Size: 2242 446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 172 2688
Size: 172 2516
AlwNI CAG_nnn'CTG
Cuts at: 0 1459 1771 2526 2688
Size: 1459 312 755 162
Fragments arranged by size:
1459 755 312 162
ApoI r'AATT_y
Cuts at: 0 475 839 996 1186 1321 2179 2688
Size: 475 364 157 190 135 858 509
Fragments arranged by size:
858 509 475 364 190 157 135
AseI AT'TA_AT
Cuts at: 0 1379 2688
Size: 1379 1309
AvaI C'yCGr_G
Cuts at: 0 8 2688
Size: 8 2680
BanI G'GyrC_C
Cuts at: 0 2401 2688
Size: 2401 287
BanII G_rGCy'C
Cuts at: 0 2051 2454 2688
Size: 2051 403 234
Fragments arranged by size:
2051 403 234
BbsI GAAGACnn'nnnn_
Cuts at: 0 1184 2688
Size: 1184 1504
BbvCI CC'TCA_GC
Cuts at: 0 128 371 2474 2569 2688
Size: 128 243 2103 95 119
Fragments arranged by size:
2103 243 128 119 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 13 47 2688
Size: 13 34 2641
Fragments arranged by size:
2641 34 13
BciVI GTATCCnnnnn_n'
Cuts at: 0 306 1217 2688
Size: 306 911 1471
Fragments arranged by size:
1471 911 306
BclI T'GATC_A
Cuts at: 0 1749 2688
Size: 1749 939
BglI GCCn_nnn'nGGC
Cuts at: 0 92 353 2688
Size: 92 261 2335
Fragments arranged by size:
2335 261 92
BglII A'GATC_T
Cuts at: 0 2545 2588 2627 2655 2688
Size: 2545 43 39 28 33
Fragments arranged by size:
2545 43 39 33 28
Bme1580I G_kGCm'C
Cuts at: 0 2358 2688
Size: 2358 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 437 1781 2504 2688
Size: 437 1344 723 184
Fragments arranged by size:
1344 723 437 184
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 644 2214 2688
Size: 644 1570 474
Fragments arranged by size:
1570 644 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 2026 2500 2688
Size: 79 1947 474 188
Fragments arranged by size:
1947 474 188 79
Bpu10I CC'TnA_GC
Cuts at: 0 128 371 2474 2569 2688
Size: 128 243 2103 95 119
Fragments arranged by size:
2103 243 128 119 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 1207 2688
Size: 1207 1481
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1668 1698 2688
Size: 1668 30 990
Fragments arranged by size:
1668 990 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 592 1643 2317 2688
Size: 145 447 1051 674 371
Fragments arranged by size:
1051 674 447 371 145
BseYI C'CCAG_C
Cuts at: 0 718 1703 2036 2383 2688
Size: 718 985 333 347 305
Fragments arranged by size:
985 718 347 333 305
BsiEI CG_ry'CG
Cuts at: 0 75 2688
Size: 75 2613
BsiHKAI G_wGCw'C
Cuts at: 0 113 188 2051 2688
Size: 113 75 1863 637
Fragments arranged by size:
1863 637 113 75
BsmI GAATG_Cn'
Cuts at: 0 332 657 2688
Size: 332 325 2031
Fragments arranged by size:
2031 332 325
Bsp1286I G_dGCh'C
Cuts at: 0 113 188 2051 2358 2454 2688
Size: 113 75 1863 307 96 234
Fragments arranged by size:
1863 307 234 113 96 75
BspHI T'CATG_A
Cuts at: 0 2106 2688
Size: 2106 582
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2534 2688
Size: 2534 154
BsrDI GCAATG_nn'
Cuts at: 0 1881 2688
Size: 1881 807
BssHII G'CGCG_C
Cuts at: 0 116 145 2688
Size: 116 29 2543
Fragments arranged by size:
2543 116 29
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2526 2688
Size: 2526 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2682 2688
Size: 2682 6
BstYI r'GATC_y
Cuts at: 0 2545 2588 2627 2655 2688
Size: 2545 43 39 28 33
Fragments arranged by size:
2545 43 39 33 28
BtgI C'CryG_G
Cuts at: 0 69 1067 1890 2675 2688
Size: 69 998 823 785 13
Fragments arranged by size:
998 823 785 69 13
BtsI GCAGTG_nn'
Cuts at: 0 29 261 1696 2491 2688
Size: 29 232 1435 795 197
Fragments arranged by size:
1435 795 232 197 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2127 2162 2688
Size: 2127 35 526
Fragments arranged by size:
2127 526 35
DraI TTT'AAA
Cuts at: 0 1062 2688
Size: 1062 1626
DraIII CAC_nnn'GTG
Cuts at: 0 574 2688
Size: 574 2114
EaeI y'GGCC_r
Cuts at: 0 72 2688
Size: 72 2616
EagI C'GGCC_G
Cuts at: 0 72 2688
Size: 72 2616
EarI CTCTTCn'nnn_
Cuts at: 0 695 812 2688
Size: 695 117 1876
Fragments arranged by size:
1876 695 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 196 2688
Size: 64 132 2492
Fragments arranged by size:
2492 132 64
EcoICRI GAG'CTC
Cuts at: 0 2049 2688
Size: 2049 639
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 890 2026 2296 2373 2500 2605 2688
Size: 79 811 1136 270 77 127 105 83
Fragments arranged by size:
1136 811 270 127 105 83 79 77
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1741 2688
Size: 84 1657 947
Fragments arranged by size:
1657 947 84
EcoRI G'AATT_C
Cuts at: 0 475 2688
Size: 475 2213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 841 1547 2117 2344 2688
Size: 841 706 570 227 344
Fragments arranged by size:
841 706 570 344 227
HaeII r_GCGC'y
Cuts at: 0 194 2688
Size: 194 2494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 828 860 1717 1749 2688
Size: 828 32 857 32 939
Fragments arranged by size:
939 857 828 32 32
HindIII A'AGCT_T
Cuts at: 0 300 1951 2688
Size: 300 1651 737
Fragments arranged by size:
1651 737 300
MfeI C'AATT_G
Cuts at: 0 1928 2688
Size: 1928 760
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2458 2663 2688
Size: 2458 205 25
Fragments arranged by size:
2458 205 25
MslI CAynn'nnrTG
Cuts at: 0 1105 2155 2242 2257 2688
Size: 1105 1050 87 15 431
Fragments arranged by size:
1105 1050 431 87 15
MspA1I CmG'CkG
Cuts at: 0 40 71 138 1195 1707 2688
Size: 40 31 67 1057 512 981
Fragments arranged by size:
1057 981 512 67 40 31
NcoI C'CATG_G
Cuts at: 0 1067 1890 2675 2688
Size: 1067 823 785 13
Fragments arranged by size:
1067 823 785 13
NspI r_CATG'y
Cuts at: 0 261 2376 2688
Size: 261 2115 312
Fragments arranged by size:
2115 312 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 799 2631 2688
Size: 799 1832 57
Fragments arranged by size:
1832 799 57
PfoI T'CCnGG_A
Cuts at: 0 2042 2688
Size: 2042 646
PpuMI rG'GwC_Cy
Cuts at: 0 1741 2688
Size: 1741 947
PstI C_TGCA'G
Cuts at: 0 161 2234 2556 2688
Size: 161 2073 322 132
Fragments arranged by size:
2073 322 161 132
PvuII CAG'CTG
Cuts at: 0 40 1195 1707 2688
Size: 40 1155 512 981
Fragments arranged by size:
1155 981 512 40
SacI G_AGCT'C
Cuts at: 0 2051 2688
Size: 2051 637
SacII CC_GC'GG
Cuts at: 0 72 2688
Size: 72 2616
SfcI C'TryA_G
Cuts at: 0 157 2230 2552 2688
Size: 157 2073 322 136
Fragments arranged by size:
2073 322 157 136
SmaI CCC'GGG
Cuts at: 0 10 2688
Size: 10 2678
SrfI GCCC'GGGC
Cuts at: 0 10 2688
Size: 10 2678
SspI AAT'ATT
Cuts at: 0 672 1942 2057 2688
Size: 672 1270 115 631
Fragments arranged by size:
1270 672 631 115
StyI C'CwwG_G
Cuts at: 0 194 251 1067 1890 2675 2688
Size: 194 57 816 823 785 13
Fragments arranged by size:
823 816 785 194 57 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2419 2657 2688
Size: 2419 238 31
Fragments arranged by size:
2419 238 31
TatI w'GTAC_w
Cuts at: 0 2234 2688
Size: 2234 454
Tth111I GACn'n_nGTC
Cuts at: 0 757 2688
Size: 757 1931
XbaI T'CTAG_A
Cuts at: 0 676 2688
Size: 676 2012
XmaI C'CCGG_G
Cuts at: 0 8 2688
Size: 8 2680
XmnI GAAnn'nnTTC
Cuts at: 0 908 1903 2100 2579 2590 2688
Size: 908 995 197 479 11 98
Fragments arranged by size:
995 908 479 197 98 11
Enzymes that do cut:
AclI AcuI AflIII AleI AlfI AlwNI ApoI AseI
AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BssHII BstAPI BstXI BstYI BtgI BtsI
CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HindIII MfeI
MmeI MslI MspA1I NcoI NspI PflMI PfoI PpuMI
PstI PvuII SacI SacII SfcI SmaI SrfI SspI
StyI TaqII TatI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BfrBI BlpI BmgBI BmtI BpuEI BsaAI BsaBI
BsaHI BsaWI BsgI BsiWI BsmBI BspEI BsrBI BsrFI
BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
DrdI EcoNI EcoRV FseI FspI FspAI HincII HpaI
KasI KpnI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI StuI
SwaI TstI XcmI XhoI ZraI