Restriction map for
pFN21ASDA0608
(Linear) (Six-base) MAPSORT of: KIAA0608.seq Check: 7096 from: 1 to: 2307
KIAA0608.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 854 2307
Size: 854 1453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 1033 2307
Size: 175 858 1274
Fragments arranged by size:
1274 858 175
AflIII A'CryG_T
Cuts at: 0 806 2307
Size: 806 1501
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 911 1734 2307
Size: 911 823 573
Fragments arranged by size:
911 823 573
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2170 2202 2307
Size: 2170 32 105
Fragments arranged by size:
2170 105 32
AlwNI CAG_nnn'CTG
Cuts at: 0 659 2307
Size: 659 1648
ApoI r'AATT_y
Cuts at: 0 1137 1144 2062 2307
Size: 1137 7 918 245
Fragments arranged by size:
1137 918 245 7
AscI GG'CGCG_CC
Cuts at: 0 719 2307
Size: 719 1588
AseI AT'TA_AT
Cuts at: 0 1618 2307
Size: 1618 689
AvaI C'yCGr_G
Cuts at: 0 30 318 393 616 636 709 911 2307
Size: 30 288 75 223 20 73 202 1396
Fragments arranged by size:
1396 288 223 202 75 73 30 20
BanI G'GyrC_C
Cuts at: 0 57 268 697 867 2307
Size: 57 211 429 170 1440
Fragments arranged by size:
1440 429 211 170 57
BanII G_rGCy'C
Cuts at: 0 236 319 325 617 918 2307
Size: 236 83 6 292 301 1389
Fragments arranged by size:
1389 301 292 236 83 6
BbeI G_GCGC'C
Cuts at: 0 272 701 2307
Size: 272 429 1606
Fragments arranged by size:
1606 429 272
BbsI GAAGACnn'nnnn_
Cuts at: 0 1115 2307
Size: 1115 1192
BbvCI CC'TCA_GC
Cuts at: 0 1259 2307
Size: 1259 1048
BglI GCCn_nnn'nGGC
Cuts at: 0 180 266 314 488 736 2307
Size: 180 86 48 174 248 1571
Fragments arranged by size:
1571 248 180 174 86 48
Bme1580I G_kGCm'C
Cuts at: 0 872 2307
Size: 872 1435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 523 2307
Size: 244 279 1784
Fragments arranged by size:
1784 279 244
Bpu10I CC'TnA_GC
Cuts at: 0 1259 2307
Size: 1259 1048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1198 1506 2307
Size: 586 612 308 801
Fragments arranged by size:
801 612 586 308
BsaHI Gr'CG_yC
Cuts at: 0 125 172 269 302 605 698 2307
Size: 125 47 97 33 303 93 1609
Fragments arranged by size:
1609 303 125 97 93 47 33
BsaWI w'CCGG_w
Cuts at: 0 9 2307
Size: 9 2298
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1089 1119 2307
Size: 1089 30 1188
Fragments arranged by size:
1188 1089 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 175 178 181 727 2307
Size: 163 12 3 3 546 1580
Fragments arranged by size:
1580 546 163 12 3 3
BseYI C'CCAG_C
Cuts at: 0 312 2307
Size: 312 1995
BsiEI CG_ry'CG
Cuts at: 0 212 298 462 492 740 2307
Size: 212 86 164 30 248 1567
Fragments arranged by size:
1567 248 212 164 86 30
BsiHKAI G_wGCw'C
Cuts at: 0 1005 2307
Size: 1005 1302
BsmBI CGTCTCn'nnnn_
Cuts at: 0 163 2307
Size: 163 2144
Bsp1286I G_dGCh'C
Cuts at: 0 236 319 325 617 872 918 1005 2307
Size: 236 83 6 292 255 46 87 1302
Fragments arranged by size:
1302 292 255 236 87 83 46 6
BspEI T'CCGG_A
Cuts at: 0 9 2307
Size: 9 2298
BspHI T'CATG_A
Cuts at: 0 1307 1683 2307
Size: 1307 376 624
Fragments arranged by size:
1307 624 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 854 2307
Size: 854 1453
BsrBI CCG'CTC
Cuts at: 0 249 2307
Size: 249 2058
BsrDI GCAATG_nn'
Cuts at: 0 1355 1760 2307
Size: 1355 405 547
Fragments arranged by size:
1355 547 405
BsrFI r'CCGG_y
Cuts at: 0 265 454 864 2307
Size: 265 189 410 1443
Fragments arranged by size:
1443 410 265 189
BssHII G'CGCG_C
Cuts at: 0 359 448 450 719 2307
Size: 359 89 2 269 1588
Fragments arranged by size:
1588 359 269 89 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1434 2307
Size: 1434 873
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1212 2307
Size: 1212 1095
BstYI r'GATC_y
Cuts at: 0 1982 2307
Size: 1982 325
BtgI C'CryG_G
Cuts at: 0 74 104 333 364 599 822 881 1449
Size: 74 30 229 31 235 223 59 568
Cuts at: 1449 2307
Size: 858
Fragments arranged by size:
858 568 235 229 223 74 59 31
30
BtsI GCAGTG_nn'
Cuts at: 0 1163 2307
Size: 1163 1144
EaeI y'GGCC_r
Cuts at: 0 209 258 354 459 489 596 737 2307
Size: 209 49 96 105 30 107 141 1570
Fragments arranged by size:
1570 209 141 107 105 96 49 30
EagI C'GGCC_G
Cuts at: 0 209 459 489 737 2307
Size: 209 250 30 248 1570
Fragments arranged by size:
1570 250 248 209 30
EarI CTCTTCn'nnn_
Cuts at: 0 425 1401 1784 2307
Size: 425 976 383 523
Fragments arranged by size:
976 523 425 383
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 134 2307
Size: 134 2173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 244 523 1033 2307
Size: 175 69 279 510 1274
Fragments arranged by size:
1274 510 279 175 69
EcoO109I rG'GnC_Cy
Cuts at: 0 508 570 706 892 1100 2307
Size: 508 62 136 186 208 1207
Fragments arranged by size:
1207 508 208 186 136 62
EcoRV GAT'ATC
Cuts at: 0 2169 2307
Size: 2169 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1933 2307
Size: 1933 374
HaeII r_GCGC'y
Cuts at: 0 134 272 701 836 2307
Size: 134 138 429 135 1471
Fragments arranged by size:
1471 429 138 135 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 162 194 541 573 810 842 1174 1206
Size: 162 32 347 32 237 32 332 32
Cuts at: 1206 2072 2104 2158 2190 2307
Size: 866 32 54 32 117
Fragments arranged by size:
866 347 332 237 162 117 54 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1220 2307
Size: 1220 1087
KasI G'GCGC_C
Cuts at: 0 268 697 2307
Size: 268 429 1610
Fragments arranged by size:
1610 429 268
MfeI C'AATT_G
Cuts at: 0 1380 2307
Size: 1380 927
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1607 2307
Size: 991 616 700
Fragments arranged by size:
991 700 616
MscI TGG'CCA
Cuts at: 0 260 2307
Size: 260 2047
MslI CAynn'nnrTG
Cuts at: 0 1716 2209 2307
Size: 1716 493 98
Fragments arranged by size:
1716 493 98
MspA1I CmG'CkG
Cuts at: 0 222 256 335 366 576 601 659 687
Size: 222 34 79 31 210 25 58 28
Cuts at: 687 824 883 2307
Size: 137 59 1424
Fragments arranged by size:
1424 222 210 137 79 59 58 34
31 28 25
NaeI GCC'GGC
Cuts at: 0 267 456 2307
Size: 267 189 1851
Fragments arranged by size:
1851 267 189
NarI GG'CG_CC
Cuts at: 0 269 698 2307
Size: 269 429 1609
Fragments arranged by size:
1609 429 269
NgoMIV G'CCGG_C
Cuts at: 0 265 454 2307
Size: 265 189 1853
Fragments arranged by size:
1853 265 189
NruI TCG'CGA
Cuts at: 0 422 2307
Size: 422 1885
NspI r_CATG'y
Cuts at: 0 1721 2307
Size: 1721 586
PacI TTA_AT'TAA
Cuts at: 0 1622 2307
Size: 1622 685
PflMI CCAn_nnn'nTGG
Cuts at: 0 2231 2307
Size: 2231 76
PpuMI rG'GwC_Cy
Cuts at: 0 570 706 892 1100 2307
Size: 570 136 186 208 1207
Fragments arranged by size:
1207 570 208 186 136
PshAI GACnn'nnGTC
Cuts at: 0 2239 2307
Size: 2239 68
PstI C_TGCA'G
Cuts at: 0 664 2120 2307
Size: 664 1456 187
Fragments arranged by size:
1456 664 187
PvuI CG_AT'CG
Cuts at: 0 298 2307
Size: 298 2009
PvuII CAG'CTG
Cuts at: 0 222 659 2307
Size: 222 437 1648
Fragments arranged by size:
1648 437 222
SacII CC_GC'GG
Cuts at: 0 336 367 602 825 884 2307
Size: 336 31 235 223 59 1423
Fragments arranged by size:
1423 336 235 223 59 31
SanDI GG'GwC_CC
Cuts at: 0 706 2307
Size: 706 1601
ScaI AGT'ACT
Cuts at: 0 1772 2307
Size: 1772 535
SfcI C'TryA_G
Cuts at: 0 660 1472 1722 2116 2307
Size: 660 812 250 394 191
Fragments arranged by size:
812 660 394 250 191
SfoI GGC'GCC
Cuts at: 0 270 699 2307
Size: 270 429 1608
Fragments arranged by size:
1608 429 270
SgrAI Cr'CCGG_yG
Cuts at: 0 454 864 2307
Size: 454 410 1443
Fragments arranged by size:
1443 454 410
SmaI CCC'GGG
Cuts at: 0 913 2307
Size: 913 1394
SmlI C'TyrA_G
Cuts at: 0 565 1177 1521 2307
Size: 565 612 344 786
Fragments arranged by size:
786 612 565 344
SrfI GCCC'GGGC
Cuts at: 0 913 2307
Size: 913 1394
SspI AAT'ATT
Cuts at: 0 1338 1882 2307
Size: 1338 544 425
Fragments arranged by size:
1338 544 425
StuI AGG'CCT
Cuts at: 0 1728 2307
Size: 1728 579
StyI C'CwwG_G
Cuts at: 0 1746 2307
Size: 1746 561
TatI w'GTAC_w
Cuts at: 0 1399 1770 2307
Size: 1399 371 537
Fragments arranged by size:
1399 537 371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1003 2307
Size: 1003 1304
XmaI C'CCGG_G
Cuts at: 0 911 2307
Size: 911 1396
XmnI GAAnn'nnTTC
Cuts at: 0 1546 2108 2307
Size: 1546 562 199
Fragments arranged by size:
1546 562 199
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApoI AscI
AseI AvaI BanI BanII BbeI BbsI BbvCI BglI
Bme1580I BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI BtgI
BtsI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRV
FalI HaeII Hin4I HindIII KasI MfeI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NruI NspI PacI
PflMI PpuMI PshAI PstI PvuI PvuII SacII SanDI
ScaI SfcI SfoI SgrAI SmaI SmlI SrfI SspI
StuI StyI TatI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI ApaI ApaLI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BsaI BsaAI BsaBI BsgI BsiWI BsmI BsrGI
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EcoICRI EcoNI EcoRI FseI FspI
FspAI HincII HpaI KpnI MluI NcoI NdeI NheI
NotI NsiI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI RsrII SacI SalI SapI
SbfI SexAI SfiI SgfI SnaBI SpeI SphI SwaI
TaqII TstI Tth111I XbaI XhoI ZraI