(Circular) (Six-base) MAPSORT of: pFN21ASDA0585.seq Check: 2709 from: 1 to: 5971 pFN21ASDA0585 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3002 4034 5418 5686 1632 Size: 1370 1032 1384 268 1917 Fragments arranged by size: 1917 1384 1370 1032 268 AfeI AGC'GCT Cuts at: 2636 2636 Size: 5971 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2607 3714 5286 2607 Size: 1107 1572 3292 Fragments arranged by size: 3292 1572 1107 ApaLI G'TGCA_C Cuts at: 3134 4028 5184 3134 Size: 894 1156 3921 Fragments arranged by size: 3921 1156 894 ApoI r'AATT_y Cuts at: 1493 2195 2774 2881 3280 3506 3658 5767 1493 Size: 702 579 107 399 226 152 2109 1697 Fragments arranged by size: 2109 1697 702 579 399 226 152 107 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2394 2685 2984 3296 1870 Size: 73 451 291 299 312 4545 Fragments arranged by size: 4545 451 312 299 291 73 BamHI G'GATC_C Cuts at: 3113 3301 3668 3113 Size: 188 367 5416 Fragments arranged by size: 5416 367 188 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2476 2640 3292 4747 618 Size: 325 217 627 689 164 652 1455 1842 Fragments arranged by size: 1842 1455 689 652 627 325 217 164 BanII G_rGCy'C Cuts at: 1391 2082 2138 3259 3290 1391 Size: 691 56 1121 31 4072 Fragments arranged by size: 4072 1121 691 56 31 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2472 928 Size: 971 573 4427 Fragments arranged by size: 4427 971 573 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 2132 3388 2132 Size: 1256 4715 Bme1580I G_kGCm'C Cuts at: 1790 3138 4032 5188 1790 Size: 1348 894 1156 2573 Fragments arranged by size: 2573 1348 1156 894 BmgBI CAC'GTC Cuts at: 1318 3159 1318 Size: 1841 4130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2583 3702 4660 Size: 416 53 83 399 24 1166 1119 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1166 1119 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2143 3238 2143 Size: 1095 4876 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 3034 3055 3061 3211 4631 1239 Size: 1064 731 21 6 150 1420 2579 Fragments arranged by size: 2579 1420 1064 731 150 21 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2066 4096 4961 5259 2066 Size: 2030 865 298 2778 Fragments arranged by size: 2778 2030 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2929 4634 882 Size: 617 1430 1705 2219 Fragments arranged by size: 2219 1705 1430 617 BsaAI yAC'GTr Cuts at: 493 1204 3268 3762 493 Size: 711 2064 494 2702 Fragments arranged by size: 2702 2064 711 494 BsaBI GATnn'nnATC Cuts at: 2582 5965 2582 Size: 3383 2588 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2567 4404 4802 5076 5223 5634 2567 Size: 1837 398 274 147 411 2904 Fragments arranged by size: 2904 1837 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2644 2674 2799 2829 3173 Size: 30 165 30 1241 30 125 30 344 Cuts at: 3173 3203 1178 Size: 30 3946 Fragments arranged by size: 3946 1241 344 165 125 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3058 3166 3214 1186 Size: 1872 108 48 3943 Fragments arranged by size: 3943 1872 108 48 BseYI C'CCAG_C Cuts at: 2513 5174 5670 2513 Size: 2661 496 2814 Fragments arranged by size: 2814 2661 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3181 1163 Size: 573 1445 3953 Fragments arranged by size: 3953 1445 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2075 2145 3421 4182 5210 1540 Size: 275 186 74 70 1276 761 1028 2301 Fragments arranged by size: 2301 1276 1028 761 275 186 74 70 BsiHKAI G_wGCw'C Cuts at: 2082 2393 2982 3138 4032 4117 5188 2082 Size: 311 589 156 894 85 1071 2865 Fragments arranged by size: 2865 1071 894 589 311 156 85 BsmI GAATG_Cn' Cuts at: 2248 2617 3482 3575 2248 Size: 369 865 93 4644 Fragments arranged by size: 4644 865 369 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2682 2682 Size: 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2138 2393 2982 3138 3259 3290 Size: 399 292 56 255 589 156 121 31 Cuts at: 3290 4032 4117 5188 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 589 399 292 255 156 121 85 56 31 BspEI T'CCGG_A Cuts at: 2567 5634 2567 Size: 3067 2904 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2206 3858 1394 Size: 429 383 1652 3507 Fragments arranged by size: 3507 1652 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4615 4802 1700 Size: 110 2805 187 2869 Fragments arranged by size: 2869 2805 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 2007 4775 2007 Size: 2768 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstXI CCAn_nnnn'nTGG Cuts at: 2513 2832 2513 Size: 319 5652 BstYI r'GATC_y Cuts at: 1972 3113 3301 3668 4053 4070 5522 5608 5620 Size: 1141 188 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1141 385 367 346 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 2229 513 Size: 552 1080 84 4255 Fragments arranged by size: 4255 1080 552 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3138 487 Size: 683 56 1912 3320 Fragments arranged by size: 3320 1912 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3203 4978 809 Size: 507 1887 1775 1802 Fragments arranged by size: 1887 1802 1775 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2072 2142 2765 Size: 54 1425 50 275 18 242 70 623 Cuts at: 2765 3418 4307 5936 8 Size: 653 889 1629 43 Fragments arranged by size: 1629 1425 889 653 623 275 242 70 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2072 2142 3418 1537 Size: 275 260 70 1276 4090 Fragments arranged by size: 4090 1276 275 260 70 EarI CTCTTCn'nnn_ Cuts at: 1353 3064 3143 3901 1353 Size: 1711 79 758 3423 Fragments arranged by size: 3423 1711 758 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2165 4558 4932 5078 387 Size: 1064 138 576 2393 374 146 1280 Fragments arranged by size: 2393 1280 1064 576 374 146 138 EcoICRI GAG'CTC Cuts at: 2080 2080 Size: 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 3002 3034 3055 3061 3211 4034 Size: 393 671 699 32 21 6 150 823 Cuts at: 4034 4631 5418 5686 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 823 787 699 671 597 393 268 150 32 21 6 EcoO109I rG'GnC_Cy Cuts at: 2529 2529 Size: 5971 EcoRI G'AATT_C Cuts at: 2774 3280 3658 2774 Size: 506 378 5087 Fragments arranged by size: 5087 506 378 FspI TGC'GCA Cuts at: 2247 2265 4477 2247 Size: 18 2212 3741 Fragments arranged by size: 3741 2212 18 HaeII r_GCGC'y Cuts at: 1678 2290 2638 5118 1678 Size: 612 348 2480 2531 Fragments arranged by size: 2531 2480 612 348 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2243 2275 2330 2362 2799 Size: 32 135 32 671 32 55 32 437 Cuts at: 2799 2831 3080 3092 3098 3112 3124 3130 3173 Size: 32 249 12 6 14 12 6 43 Cuts at: 3173 3205 4612 4644 4686 4718 1373 Size: 32 1407 32 42 32 2626 Fragments arranged by size: 2626 1407 671 437 249 135 55 43 42 32 32 32 32 32 32 32 32 14 12 12 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3013 3315 3561 669 Size: 988 281 1075 302 246 3079 Fragments arranged by size: 3079 1075 988 302 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2783 3066 3117 5085 5269 1305 Size: 1478 283 51 1968 184 2007 Fragments arranged by size: 2007 1968 1478 283 184 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 2767 10 Size: 54 1425 343 935 3214 Fragments arranged by size: 3214 1425 935 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2678 3929 4288 4447 518 Size: 648 1512 1251 359 159 2042 Fragments arranged by size: 2042 1512 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 3387 4064 5212 5457 1606 Size: 576 1205 677 1148 245 2120 Fragments arranged by size: 2120 1205 1148 677 576 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5419 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 2843 3329 4874 1132 Size: 97 1614 486 1545 2229 Fragments arranged by size: 2229 1614 1545 486 97 PasI CC'CwG_GG Cuts at: 1348 2664 1348 Size: 1316 4655 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 2607 1108 Size: 1499 4472 PfoI T'CCnGG_A Cuts at: 3227 3227 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 3323 830 Size: 2493 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2182 2182 Size: 5971 SacI G_AGCT'C Cuts at: 2082 2082 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SexAI A'CCwGG_T Cuts at: 2723 2723 Size: 5971 SfcI C'TryA_G Cuts at: 826 1045 3319 4454 5135 5326 826 Size: 219 2274 1135 681 191 1471 Fragments arranged by size: 2274 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2081 2394 4075 4976 5238 820 Size: 197 926 138 313 1681 901 262 1553 Fragments arranged by size: 1681 1553 926 901 313 262 197 138 SnaBI TAC'GTA Cuts at: 493 3268 3762 493 Size: 2775 494 2702 Fragments arranged by size: 2775 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 3410 513 Size: 552 2345 3074 Fragments arranged by size: 3074 2345 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2891 3998 4015 4168 4353 4798 325 Size: 1255 1311 1107 17 153 185 445 1498 Fragments arranged by size: 1498 1311 1255 1107 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 4217 5929 Size: 274 80 33 51 1443 402 1838 1712 Cuts at: 5929 96 Size: 138 Fragments arranged by size: 1838 1712 1443 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 3203 1316 Size: 1887 4084 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2832 2905 2832 Size: 73 5898 XhoI C'TCGA_G Cuts at: 1943 2394 1943 Size: 451 5520 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI